Vernonia yellow vein Fujian betasatellite
Average proteome isoelectric point is 6.3
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|G1C5E5|G1C5E5_9VIRU C1 protein OS=Vernonia yellow vein Fujian betasatellite OX=2050589 PE=4 SV=1
MM1 pKa = 7.24 NNKK4 pKa = 9.62 SSNHH8 pKa = 5.14 QSKK11 pKa = 10.59 QMTITHH17 pKa = 5.89 TTNKK21 pKa = 9.78 GIRR24 pKa = 11.84 FVFSLQKK31 pKa = 10.1 KK32 pKa = 7.82 QEE34 pKa = 4.13 HH35 pKa = 6.17 FLVTVKK41 pKa = 10.66 VINTSSPVLSKK52 pKa = 9.25 HH53 pKa = 5.28 TYY55 pKa = 9.0 KK56 pKa = 10.15 IRR58 pKa = 11.84 YY59 pKa = 8.07 DD60 pKa = 3.96 YY61 pKa = 10.88 QWNAVPFDD69 pKa = 4.22 FNGLEE74 pKa = 3.91 TSMKK78 pKa = 9.07 NTIEE82 pKa = 4.05 VLYY85 pKa = 10.54 HH86 pKa = 6.57 DD87 pKa = 4.91 SSYY90 pKa = 11.82 NDD92 pKa = 3.25 FKK94 pKa = 11.19 EE95 pKa = 4.06 EE96 pKa = 3.87 NMIDD100 pKa = 3.99 AIDD103 pKa = 4.03 TIMFDD108 pKa = 3.39 TNMILGVEE116 pKa = 4.36 IIEE119 pKa = 4.25 PHH121 pKa = 5.66 TCTTSRR127 pKa = 11.84 III129 pKa = 4.3
Molecular weight: 15.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.303
IPC2_protein 6.148
IPC_protein 6.351
Toseland 6.825
ProMoST 6.707
Dawson 6.693
Bjellqvist 6.576
Wikipedia 6.722
Rodwell 6.678
Grimsley 6.985
Solomon 6.707
Lehninger 6.707
Nozaki 6.912
DTASelect 7.117
Thurlkill 7.161
EMBOSS 7.161
Sillero 7.059
Patrickios 4.253
IPC_peptide 6.722
IPC2_peptide 6.883
IPC2.peptide.svr19 6.886
Protein with the highest isoelectric point:
>tr|G1C5E5|G1C5E5_9VIRU C1 protein OS=Vernonia yellow vein Fujian betasatellite OX=2050589 PE=4 SV=1
MM1 pKa = 7.24 NNKK4 pKa = 9.62 SSNHH8 pKa = 5.14 QSKK11 pKa = 10.59 QMTITHH17 pKa = 5.89 TTNKK21 pKa = 9.78 GIRR24 pKa = 11.84 FVFSLQKK31 pKa = 10.1 KK32 pKa = 7.82 QEE34 pKa = 4.13 HH35 pKa = 6.17 FLVTVKK41 pKa = 10.66 VINTSSPVLSKK52 pKa = 9.25 HH53 pKa = 5.28 TYY55 pKa = 9.0 KK56 pKa = 10.15 IRR58 pKa = 11.84 YY59 pKa = 8.07 DD60 pKa = 3.96 YY61 pKa = 10.88 QWNAVPFDD69 pKa = 4.22 FNGLEE74 pKa = 3.91 TSMKK78 pKa = 9.07 NTIEE82 pKa = 4.05 VLYY85 pKa = 10.54 HH86 pKa = 6.57 DD87 pKa = 4.91 SSYY90 pKa = 11.82 NDD92 pKa = 3.25 FKK94 pKa = 11.19 EE95 pKa = 4.06 EE96 pKa = 3.87 NMIDD100 pKa = 3.99 AIDD103 pKa = 4.03 TIMFDD108 pKa = 3.39 TNMILGVEE116 pKa = 4.36 IIEE119 pKa = 4.25 PHH121 pKa = 5.66 TCTTSRR127 pKa = 11.84 III129 pKa = 4.3
Molecular weight: 15.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.303
IPC2_protein 6.148
IPC_protein 6.351
Toseland 6.825
ProMoST 6.707
Dawson 6.693
Bjellqvist 6.576
Wikipedia 6.722
Rodwell 6.678
Grimsley 6.985
Solomon 6.707
Lehninger 6.707
Nozaki 6.912
DTASelect 7.117
Thurlkill 7.161
EMBOSS 7.161
Sillero 7.059
Patrickios 4.253
IPC_peptide 6.722
IPC2_peptide 6.883
IPC2.peptide.svr19 6.886
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
129
129
129
129.0
15.04
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
1.55 ± 0.0
0.775 ± 0.0
5.426 ± 0.0
5.426 ± 0.0
5.426 ± 0.0
2.326 ± 0.0
4.651 ± 0.0
10.078 ± 0.0
7.752 ± 0.0
4.651 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
4.651 ± 0.0
8.527 ± 0.0
2.326 ± 0.0
3.876 ± 0.0
2.326 ± 0.0
8.527 ± 0.0
10.853 ± 0.0
6.202 ± 0.0
0.775 ± 0.0
3.876 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here