Streptococcus phage IPP23
Average proteome isoelectric point is 6.39
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1S5S9Z1|A0A1S5S9Z1_9CAUD Replication protein OS=Streptococcus phage IPP23 OX=1916163 GN=IPP23_00012 PE=4 SV=1
MM1 pKa = 7.73 LAFNLLKK8 pKa = 9.5 PTSNGEE14 pKa = 3.97 NEE16 pKa = 3.98 LGQEE20 pKa = 3.55 IFEE23 pKa = 4.38 YY24 pKa = 10.45 KK25 pKa = 9.76 QVAVFEE31 pKa = 5.43 GYY33 pKa = 9.64 MDD35 pKa = 3.76 MLDD38 pKa = 3.78 GSEE41 pKa = 4.3 STDD44 pKa = 3.05 KK45 pKa = 10.9 LAYY48 pKa = 9.56 IADD51 pKa = 3.94 STHH54 pKa = 7.01 VILTKK59 pKa = 10.75 DD60 pKa = 2.97 RR61 pKa = 11.84 TVNAEE66 pKa = 3.83 IEE68 pKa = 4.37 DD69 pKa = 4.2 KK70 pKa = 11.24 IEE72 pKa = 4.24 VNGKK76 pKa = 6.8 TYY78 pKa = 10.62 EE79 pKa = 3.96 VTYY82 pKa = 11.13 VDD84 pKa = 3.99 DD85 pKa = 4.17 PVNIGHH91 pKa = 6.9 HH92 pKa = 5.69 LEE94 pKa = 4.26 IYY96 pKa = 10.61 VKK98 pKa = 10.44 GAHH101 pKa = 6.02
Molecular weight: 11.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.449
IPC2_protein 4.533
IPC_protein 4.418
Toseland 4.279
ProMoST 4.507
Dawson 4.38
Bjellqvist 4.52
Wikipedia 4.253
Rodwell 4.266
Grimsley 4.19
Solomon 4.368
Lehninger 4.329
Nozaki 4.495
DTASelect 4.635
Thurlkill 4.291
EMBOSS 4.266
Sillero 4.546
Patrickios 3.452
IPC_peptide 4.38
IPC2_peptide 4.533
IPC2.peptide.svr19 4.476
Protein with the highest isoelectric point:
>tr|A0A1S5S9Z2|A0A1S5S9Z2_9CAUD Uncharacterized protein OS=Streptococcus phage IPP23 OX=1916163 GN=IPP23_00027 PE=4 SV=1
MM1 pKa = 7.1 VVLANHH7 pKa = 6.84 PNWQVYY13 pKa = 9.28 PEE15 pKa = 4.76 EE16 pKa = 3.7 IAKK19 pKa = 10.29 RR20 pKa = 11.84 KK21 pKa = 9.12 GVSRR25 pKa = 11.84 PTVSKK30 pKa = 10.09 YY31 pKa = 10.65 FKK33 pKa = 9.88 ILEE36 pKa = 3.99 EE37 pKa = 4.49 AGYY40 pKa = 10.18 LRR42 pKa = 11.84 QVRR45 pKa = 11.84 RR46 pKa = 11.84 KK47 pKa = 8.3 PPGRR51 pKa = 11.84 GGSHH55 pKa = 5.6 VFRR58 pKa = 11.84 FFSDD62 pKa = 3.14 RR63 pKa = 11.84 KK64 pKa = 10.1 ISDD67 pKa = 3.5 FQFDD71 pKa = 3.15 IMLQRR76 pKa = 11.84 LDD78 pKa = 3.83 KK79 pKa = 10.89 AISDD83 pKa = 3.93 STFEE87 pKa = 3.85 II88 pKa = 4.66
Molecular weight: 10.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.137
IPC2_protein 9.355
IPC_protein 9.487
Toseland 10.204
ProMoST 9.809
Dawson 10.35
Bjellqvist 9.984
Wikipedia 10.496
Rodwell 10.774
Grimsley 10.409
Solomon 10.394
Lehninger 10.365
Nozaki 10.16
DTASelect 9.984
Thurlkill 10.218
EMBOSS 10.584
Sillero 10.262
Patrickios 10.526
IPC_peptide 10.394
IPC2_peptide 8.478
IPC2.peptide.svr19 8.494
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
54
0
54
10310
44
664
190.9
21.75
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.411 ± 0.333
0.601 ± 0.085
6.266 ± 0.259
8.264 ± 0.367
4.287 ± 0.25
5.829 ± 0.374
1.474 ± 0.127
7.546 ± 0.25
8.739 ± 0.456
8.555 ± 0.393
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.599 ± 0.195
5.761 ± 0.246
2.473 ± 0.14
3.686 ± 0.198
4.268 ± 0.33
6.217 ± 0.285
6.198 ± 0.416
5.82 ± 0.268
1.28 ± 0.173
3.725 ± 0.285
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here