Chilli leaf curl India virus
Average proteome isoelectric point is 7.75
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|D3UBT0|D3UBT0_9GEMI Capsid protein OS=Chilli leaf curl India virus OX=577124 GN=AV1 PE=3 SV=1
MM1 pKa = 7.99 WDD3 pKa = 3.38 PLVNEE8 pKa = 4.72 FPEE11 pKa = 4.61 TVHH14 pKa = 7.0 GFRR17 pKa = 11.84 CMLAVKK23 pKa = 9.35 YY24 pKa = 10.52 LRR26 pKa = 11.84 LVEE29 pKa = 4.16 NTYY32 pKa = 11.2 SPDD35 pKa = 3.18 TLGYY39 pKa = 10.84 DD40 pKa = 4.83 LIRR43 pKa = 11.84 DD44 pKa = 4.71 LISVIRR50 pKa = 11.84 ARR52 pKa = 11.84 NYY54 pKa = 9.49 VEE56 pKa = 3.52 ATSRR60 pKa = 11.84 YY61 pKa = 6.98 NHH63 pKa = 5.62 FHH65 pKa = 6.55 ARR67 pKa = 11.84 LEE69 pKa = 4.15 GTPPSEE75 pKa = 3.81 LRR77 pKa = 11.84 QPICEE82 pKa = 4.2 PCCCPHH88 pKa = 7.09 CPRR91 pKa = 11.84 HH92 pKa = 6.04 KK93 pKa = 9.6 GKK95 pKa = 11.23 SMGEE99 pKa = 3.81 QAHH102 pKa = 5.83 EE103 pKa = 4.34 PKK105 pKa = 10.7 AQDD108 pKa = 3.19 VQGVQKK114 pKa = 9.76 PRR116 pKa = 11.84 CSEE119 pKa = 3.87 GMM121 pKa = 3.46
Molecular weight: 13.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.434
IPC2_protein 6.427
IPC_protein 6.576
Toseland 6.561
ProMoST 6.927
Dawson 7.088
Bjellqvist 6.985
Wikipedia 7.044
Rodwell 7.073
Grimsley 6.634
Solomon 7.117
Lehninger 7.132
Nozaki 7.351
DTASelect 7.38
Thurlkill 7.41
EMBOSS 7.439
Sillero 7.527
Patrickios 3.719
IPC_peptide 7.132
IPC2_peptide 7.249
IPC2.peptide.svr19 7.065
Protein with the highest isoelectric point:
>tr|D3UBT1|D3UBT1_9GEMI Replication enhancer OS=Chilli leaf curl India virus OX=577124 GN=AC3 PE=3 SV=1
MM1 pKa = 7.71 SKK3 pKa = 10.35 RR4 pKa = 11.84 PADD7 pKa = 3.87 IIISTPASKK16 pKa = 10.3 VRR18 pKa = 11.84 RR19 pKa = 11.84 RR20 pKa = 11.84 LNFDD24 pKa = 2.99 SPYY27 pKa = 10.47 ASRR30 pKa = 11.84 AAAPIVRR37 pKa = 11.84 VTKK40 pKa = 10.52 ARR42 pKa = 11.84 AWANRR47 pKa = 11.84 PMNRR51 pKa = 11.84 KK52 pKa = 7.69 PKK54 pKa = 8.38 MYY56 pKa = 9.58 RR57 pKa = 11.84 VYY59 pKa = 10.61 RR60 pKa = 11.84 SPDD63 pKa = 3.13 VPRR66 pKa = 11.84 GCEE69 pKa = 4.34 GPCKK73 pKa = 10.01 VQSFEE78 pKa = 4.1 SRR80 pKa = 11.84 HH81 pKa = 6.13 DD82 pKa = 3.26 IQHH85 pKa = 6.35 IGKK88 pKa = 8.97 VMCVSDD94 pKa = 3.5 VTRR97 pKa = 11.84 GIGLTHH103 pKa = 6.67 RR104 pKa = 11.84 VGKK107 pKa = 9.66 RR108 pKa = 11.84 FCVKK112 pKa = 9.94 SVYY115 pKa = 10.52 VLGKK119 pKa = 9.41 IWMDD123 pKa = 3.35 EE124 pKa = 4.04 NIKK127 pKa = 9.32 TKK129 pKa = 10.6 NHH131 pKa = 5.78 TNSVMFFLVRR141 pKa = 11.84 DD142 pKa = 3.84 RR143 pKa = 11.84 RR144 pKa = 11.84 PVDD147 pKa = 3.13 KK148 pKa = 10.28 PQDD151 pKa = 3.56 FGEE154 pKa = 4.33 VFNMFDD160 pKa = 4.29 NEE162 pKa = 4.11 PSTATVKK169 pKa = 10.38 NVHH172 pKa = 6.5 RR173 pKa = 11.84 DD174 pKa = 3.15 RR175 pKa = 11.84 YY176 pKa = 8.56 QVLRR180 pKa = 11.84 KK181 pKa = 8.43 WHH183 pKa = 5.58 ATVTGGQYY191 pKa = 10.85 ASKK194 pKa = 10.24 EE195 pKa = 3.73 QALVKK200 pKa = 10.65 KK201 pKa = 9.58 FIRR204 pKa = 11.84 VNNYY208 pKa = 7.74 VVYY211 pKa = 9.96 NQQEE215 pKa = 3.8 AGKK218 pKa = 10.1 YY219 pKa = 8.23 EE220 pKa = 4.02 NHH222 pKa = 6.47 TEE224 pKa = 3.99 NALMLYY230 pKa = 7.52 MACTHH235 pKa = 7.06 ASNPVYY241 pKa = 9.86 ATLKK245 pKa = 9.47 IRR247 pKa = 11.84 IYY249 pKa = 10.67 FYY251 pKa = 11.26 DD252 pKa = 3.51 SVSNN256 pKa = 3.9
Molecular weight: 29.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.251
IPC2_protein 9.516
IPC_protein 9.692
Toseland 10.145
ProMoST 9.853
Dawson 10.35
Bjellqvist 10.028
Wikipedia 10.526
Rodwell 10.73
Grimsley 10.423
Solomon 10.379
Lehninger 10.335
Nozaki 10.16
DTASelect 10.028
Thurlkill 10.204
EMBOSS 10.555
Sillero 10.262
Patrickios 10.292
IPC_peptide 10.365
IPC2_peptide 8.858
IPC2.peptide.svr19 8.469
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1083
85
352
180.5
20.66
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.725 ± 0.787
2.124 ± 0.466
4.801 ± 0.482
4.801 ± 0.548
4.155 ± 0.594
4.801 ± 0.574
4.155 ± 0.617
5.263 ± 0.588
6.002 ± 0.823
6.094 ± 0.743
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.77 ± 0.77
5.448 ± 0.625
6.925 ± 0.743
5.078 ± 0.696
6.741 ± 1.142
8.033 ± 1.507
4.801 ± 0.536
7.11 ± 1.516
1.385 ± 0.21
3.786 ± 0.549
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here