Arabidopsis halleri partitivirus 1
Average proteome isoelectric point is 6.94
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1B4ZBZ5|A0A1B4ZBZ5_9VIRU RNA-dependent RNA polymerase OS=Arabidopsis halleri partitivirus 1 OX=1849335 GN=RdRP PE=4 SV=1
MM1 pKa = 7.43 SMKK4 pKa = 10.24 RR5 pKa = 11.84 KK6 pKa = 9.39 SRR8 pKa = 11.84 PSSSTKK14 pKa = 10.38 DD15 pKa = 3.41 LEE17 pKa = 5.09 VEE19 pKa = 3.96 DD20 pKa = 5.1 TYY22 pKa = 11.65 LKK24 pKa = 10.72 QSGLDD29 pKa = 3.42 SMNKK33 pKa = 9.91 LEE35 pKa = 4.82 PVEE38 pKa = 3.93 QSKK41 pKa = 11.44 DD42 pKa = 3.37 EE43 pKa = 4.21 EE44 pKa = 4.54 TTKK47 pKa = 10.88 VSMLPTASTAIIAPRR62 pKa = 11.84 KK63 pKa = 8.23 LTAEE67 pKa = 4.39 DD68 pKa = 4.62 FSSKK72 pKa = 10.24 RR73 pKa = 11.84 KK74 pKa = 9.19 PDD76 pKa = 3.21 QTSAVSPFFGFLRR89 pKa = 11.84 THH91 pKa = 7.03 ILHH94 pKa = 6.42 PTQGRR99 pKa = 11.84 LSHH102 pKa = 6.45 YY103 pKa = 10.22 YY104 pKa = 9.41 PSCHH108 pKa = 5.63 MMDD111 pKa = 4.8 YY112 pKa = 10.11 ILHH115 pKa = 6.77 SINSTLCDD123 pKa = 2.82 NYY125 pKa = 10.2 YY126 pKa = 10.63 FKK128 pKa = 11.15 RR129 pKa = 11.84 EE130 pKa = 3.91 TPNYY134 pKa = 8.87 HH135 pKa = 6.8 PYY137 pKa = 10.43 ILRR140 pKa = 11.84 LYY142 pKa = 10.19 FGVLFWVQCLRR153 pKa = 11.84 AGNDD157 pKa = 3.18 VQVINDD163 pKa = 3.34 LHH165 pKa = 7.93 YY166 pKa = 11.44 DD167 pKa = 4.01 FLQRR171 pKa = 11.84 FLDD174 pKa = 4.13 CNPLEE179 pKa = 4.41 SLAIPGPLLGLFKK192 pKa = 9.67 TLCSSQPEE200 pKa = 4.17 FPHH203 pKa = 5.86 YY204 pKa = 10.63 GKK206 pKa = 10.64 VYY208 pKa = 9.91 PRR210 pKa = 11.84 IPASPGPRR218 pKa = 11.84 RR219 pKa = 11.84 RR220 pKa = 11.84 DD221 pKa = 3.25 MFSKK225 pKa = 10.47 NVPSAQFLPNVPGIFALIHH244 pKa = 5.98 HH245 pKa = 6.77 LHH247 pKa = 6.2 GLSEE251 pKa = 4.65 GEE253 pKa = 3.83 HH254 pKa = 6.64 PIYY257 pKa = 10.23 PKK259 pKa = 10.2 RR260 pKa = 11.84 KK261 pKa = 7.47 RR262 pKa = 11.84 HH263 pKa = 5.46 IPVTEE268 pKa = 3.76 EE269 pKa = 3.43 ASNFGFKK276 pKa = 10.56 AFAAFPNRR284 pKa = 11.84 IQRR287 pKa = 11.84 DD288 pKa = 3.17 RR289 pKa = 11.84 WMVSSPGLQYY299 pKa = 10.71 PCEE302 pKa = 4.66 ADD304 pKa = 2.91 MKK306 pKa = 9.69 MNEE309 pKa = 3.76 AFAEE313 pKa = 4.02 RR314 pKa = 11.84 FYY316 pKa = 11.57 DD317 pKa = 3.77 FDD319 pKa = 4.21 FPAFNADD326 pKa = 4.32 DD327 pKa = 4.07 NLSTITNFLHH337 pKa = 5.97 MRR339 pKa = 11.84 KK340 pKa = 9.92 SMAWFIRR347 pKa = 11.84 AKK349 pKa = 10.3 EE350 pKa = 4.26 VACSAARR357 pKa = 11.84 FFSDD361 pKa = 3.16 SGTLADD367 pKa = 4.81 CSPHH371 pKa = 7.26 GLVSNQIIVAITPPPEE387 pKa = 3.72 EE388 pKa = 4.13 TFADD392 pKa = 3.89 PRR394 pKa = 11.84 FSADD398 pKa = 3.13 PRR400 pKa = 11.84 ALYY403 pKa = 9.71 PFSFKK408 pKa = 10.86 LKK410 pKa = 9.08 STAHH414 pKa = 6.25 NLPPLAEE421 pKa = 3.95 AAAAFSQTHH430 pKa = 5.05 IRR432 pKa = 11.84 IFPEE436 pKa = 3.82 YY437 pKa = 9.33 PLAGNFGQKK446 pKa = 8.36 TDD448 pKa = 3.4 EE449 pKa = 5.36 SGPFWDD455 pKa = 4.14 IRR457 pKa = 11.84 PIGSSPTDD465 pKa = 3.26 DD466 pKa = 3.27 TSYY469 pKa = 10.27 LTIPPMVKK477 pKa = 9.91 AALIEE482 pKa = 4.54 KK483 pKa = 10.19 GSSRR487 pKa = 4.02
Molecular weight: 55.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.906
IPC2_protein 7.176
IPC_protein 7.146
Toseland 7.044
ProMoST 7.79
Dawson 7.79
Bjellqvist 8.039
Wikipedia 7.717
Rodwell 7.79
Grimsley 7.146
Solomon 7.849
Lehninger 7.863
Nozaki 8.244
DTASelect 7.951
Thurlkill 7.995
EMBOSS 8.039
Sillero 8.258
Patrickios 4.266
IPC_peptide 7.849
IPC2_peptide 7.351
IPC2.peptide.svr19 7.245
Protein with the highest isoelectric point:
>tr|A0A1B4ZBZ5|A0A1B4ZBZ5_9VIRU RNA-dependent RNA polymerase OS=Arabidopsis halleri partitivirus 1 OX=1849335 GN=RdRP PE=4 SV=1
MM1 pKa = 7.56 KK2 pKa = 9.43 NTVVLEE8 pKa = 4.09 PLPSLARR15 pKa = 11.84 PIYY18 pKa = 10.49 GDD20 pKa = 3.55 TDD22 pKa = 3.77 PGRR25 pKa = 11.84 NPAYY29 pKa = 10.31 QSTVDD34 pKa = 3.16 HH35 pKa = 6.79 ALRR38 pKa = 11.84 RR39 pKa = 11.84 LLTAEE44 pKa = 3.96 EE45 pKa = 4.14 FNIVVNGYY53 pKa = 9.44 RR54 pKa = 11.84 RR55 pKa = 11.84 SPWNEE60 pKa = 3.72 DD61 pKa = 2.95 ALTADD66 pKa = 3.57 IEE68 pKa = 4.44 KK69 pKa = 10.85 LNSDD73 pKa = 3.74 YY74 pKa = 11.62 HH75 pKa = 6.85 HH76 pKa = 6.71 VNKK79 pKa = 10.51 DD80 pKa = 2.94 EE81 pKa = 4.72 HH82 pKa = 6.65 YY83 pKa = 9.72 YY84 pKa = 10.74 KK85 pKa = 10.63 AIEE88 pKa = 4.11 HH89 pKa = 5.6 TKK91 pKa = 10.89 KK92 pKa = 10.78 LFTPKK97 pKa = 10.17 EE98 pKa = 3.93 KK99 pKa = 10.52 LRR101 pKa = 11.84 PVHH104 pKa = 6.84 FNDD107 pKa = 3.23 LRR109 pKa = 11.84 HH110 pKa = 5.85 YY111 pKa = 8.77 PWQLSTSIGAPFATSEE127 pKa = 3.82 KK128 pKa = 8.85 WKK130 pKa = 10.72 DD131 pKa = 3.83 YY132 pKa = 11.16 INQKK136 pKa = 10.46 YY137 pKa = 10.05 DD138 pKa = 3.17 GKK140 pKa = 11.0 LKK142 pKa = 10.99 SRR144 pKa = 11.84 DD145 pKa = 3.55 FKK147 pKa = 11.7 DD148 pKa = 3.52 LFKK151 pKa = 9.88 EE152 pKa = 4.04 THH154 pKa = 5.7 GVSLEE159 pKa = 3.84 PYY161 pKa = 8.96 MIDD164 pKa = 3.32 RR165 pKa = 11.84 RR166 pKa = 11.84 LSKK169 pKa = 10.8 RR170 pKa = 11.84 NFYY173 pKa = 11.49 NEE175 pKa = 3.38 MFYY178 pKa = 10.66 INRR181 pKa = 11.84 INIHH185 pKa = 6.42 HH186 pKa = 6.94 IKK188 pKa = 10.66 DD189 pKa = 3.26 GWTTNPAGHH198 pKa = 7.47 DD199 pKa = 3.22 LRR201 pKa = 11.84 YY202 pKa = 8.96 WHH204 pKa = 6.54 TAHH207 pKa = 7.24 ARR209 pKa = 11.84 QHH211 pKa = 5.72 LVEE214 pKa = 6.38 AGDD217 pKa = 3.69 EE218 pKa = 4.35 DD219 pKa = 4.26 KK220 pKa = 11.53 VRR222 pKa = 11.84 LVFGAPSTLLMAEE235 pKa = 5.16 LMFIWPIQTSLLARR249 pKa = 11.84 GSSSPMLWGYY259 pKa = 7.61 EE260 pKa = 4.06 TTTGGWSRR268 pKa = 11.84 LYY270 pKa = 10.23 NWAYY274 pKa = 10.43 SALPRR279 pKa = 11.84 FGAVATLDD287 pKa = 3.16 WSRR290 pKa = 11.84 FDD292 pKa = 3.12 KK293 pKa = 10.81 DD294 pKa = 3.13 ARR296 pKa = 11.84 HH297 pKa = 5.2 TVITDD302 pKa = 2.81 IHH304 pKa = 6.94 DD305 pKa = 4.97 LIMRR309 pKa = 11.84 PMFDD313 pKa = 3.84 FNSGYY318 pKa = 10.28 HH319 pKa = 4.96 PTIINPRR326 pKa = 11.84 SNPDD330 pKa = 3.29 PQRR333 pKa = 11.84 LEE335 pKa = 4.85 NLWNWMKK342 pKa = 10.84 NAILTTPLLLPDD354 pKa = 3.76 GTRR357 pKa = 11.84 LQFQHH362 pKa = 6.68 SGIYY366 pKa = 9.69 SGYY369 pKa = 9.65 FQTQILDD376 pKa = 3.45 SMYY379 pKa = 8.75 NCVMIFTVLSRR390 pKa = 11.84 MGFDD394 pKa = 3.97 LNSVAIKK401 pKa = 10.63 VQGDD405 pKa = 3.5 DD406 pKa = 3.71 SLILLSHH413 pKa = 7.04 SYY415 pKa = 9.96 TFLQHH420 pKa = 6.93 SFLTTFAHH428 pKa = 6.42 HH429 pKa = 6.22 AAVYY433 pKa = 9.76 FGSTLNVKK441 pKa = 10.18 KK442 pKa = 10.91 SEE444 pKa = 4.25 LLPSLEE450 pKa = 4.12 DD451 pKa = 3.72 AEE453 pKa = 4.33 VLRR456 pKa = 11.84 YY457 pKa = 9.56 RR458 pKa = 11.84 NHH460 pKa = 6.97 GMMPYY465 pKa = 9.77 RR466 pKa = 11.84 EE467 pKa = 4.22 EE468 pKa = 4.12 LQLLAMLRR476 pKa = 11.84 HH477 pKa = 6.2 PEE479 pKa = 3.89 RR480 pKa = 11.84 TASLSALMARR490 pKa = 11.84 SIGIAYY496 pKa = 8.7 ANCGNYY502 pKa = 8.65 TRR504 pKa = 11.84 VHH506 pKa = 6.83 HH507 pKa = 6.31 ICEE510 pKa = 5.19 DD511 pKa = 2.66 IHH513 pKa = 7.94 NYY515 pKa = 10.23 LKK517 pKa = 11.13 GIGVKK522 pKa = 9.77 PDD524 pKa = 3.22 AFGLPGGLRR533 pKa = 11.84 FRR535 pKa = 11.84 KK536 pKa = 9.87 NYY538 pKa = 9.55 LPSYY542 pKa = 9.24 EE543 pKa = 4.96 EE544 pKa = 3.73 IDD546 pKa = 3.32 ISHH549 pKa = 7.06 FPTWLEE555 pKa = 3.95 TVEE558 pKa = 4.88 RR559 pKa = 11.84 LLDD562 pKa = 3.69 PSRR565 pKa = 11.84 PLLTNKK571 pKa = 9.32 HH572 pKa = 5.29 WPTTHH577 pKa = 6.79 FFGIPGEE584 pKa = 4.37 SS585 pKa = 3.11
Molecular weight: 67.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.98
IPC2_protein 7.19
IPC_protein 7.322
Toseland 7.527
ProMoST 7.658
Dawson 7.878
Bjellqvist 7.834
Wikipedia 7.878
Rodwell 7.878
Grimsley 7.688
Solomon 7.995
Lehninger 7.98
Nozaki 7.98
DTASelect 8.024
Thurlkill 8.068
EMBOSS 8.17
Sillero 8.185
Patrickios 4.228
IPC_peptide 7.98
IPC2_peptide 7.351
IPC2.peptide.svr19 7.237
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1072
487
585
536.0
61.43
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.716 ± 0.645
1.026 ± 0.366
5.69 ± 0.087
4.944 ± 0.009
5.784 ± 0.833
4.944 ± 0.375
4.384 ± 0.531
5.317 ± 0.109
4.851 ± 0.168
9.701 ± 0.883
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.705 ± 0.021
4.478 ± 0.464
7.369 ± 1.111
2.519 ± 0.211
6.063 ± 0.186
7.836 ± 0.834
5.877 ± 0.441
3.731 ± 0.143
1.679 ± 0.509
4.384 ± 0.409
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here