Streptococcus phage Javan191

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6A3J2|A0A4D6A3J2_9CAUD Uncharacterized protein OS=Streptococcus phage Javan191 OX=2548039 GN=Javan191_0008 PE=4 SV=1
MM1 pKa = 8.26DD2 pKa = 6.9DD3 pKa = 3.13IQAPPTGTKK12 pKa = 8.86GTVRR16 pKa = 11.84GVDD19 pKa = 3.87DD20 pKa = 4.31IGSIMVSWDD29 pKa = 3.31NGSSLSLAYY38 pKa = 10.16GVDD41 pKa = 3.28TFRR44 pKa = 11.84RR45 pKa = 11.84CDD47 pKa = 4.03DD48 pKa = 3.66EE49 pKa = 6.64SEE51 pKa = 4.26DD52 pKa = 3.68

Molecular weight: 5.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6A3K0|A0A4D6A3K0_9CAUD Putative DNA packaging protein OS=Streptococcus phage Javan191 OX=2548039 GN=Javan191_0033 PE=4 SV=1
MM1 pKa = 7.62ILLLMRR7 pKa = 11.84QQDD10 pKa = 3.07IFLQTDD16 pKa = 3.72YY17 pKa = 11.27VKK19 pKa = 10.71QRR21 pKa = 11.84ISLIEE26 pKa = 3.71RR27 pKa = 11.84SGAYY31 pKa = 9.75DD32 pKa = 3.64DD33 pKa = 3.91RR34 pKa = 11.84TKK36 pKa = 11.04GGQRR40 pKa = 3.24

Molecular weight: 4.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

13139

40

941

238.9

27.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.865 ± 0.336

0.967 ± 0.111

6.363 ± 0.353

8.174 ± 0.39

3.958 ± 0.184

6.416 ± 0.38

1.575 ± 0.113

7.649 ± 0.209

8.578 ± 0.269

8.197 ± 0.286

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.732 ± 0.199

4.841 ± 0.223

3.143 ± 0.202

2.831 ± 0.162

4.574 ± 0.295

6.751 ± 0.389

5.16 ± 0.268

6.142 ± 0.249

1.119 ± 0.108

3.965 ± 0.307
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski