Amino acid dipepetide frequency for Nitrospirales bacterium LBB_01

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.019AlaAla: 7.019 ± 0.149	0.719AlaCys: 0.719 ± 0.034	3.764AlaAsp: 3.764 ± 0.081	4.455AlaGlu: 4.455 ± 0.082	3.205AlaPhe: 3.205 ± 0.081	5.027AlaGly: 5.027 ± 0.096	1.36AlaHis: 1.36 ± 0.046	5.789AlaIle: 5.789 ± 0.107	4.498AlaLys: 4.498 ± 0.101	8.288AlaLeu: 8.288 ± 0.112	2.214AlaMet: 2.214 ± 0.048	2.458AlaAsn: 2.458 ± 0.07	1.859AlaPro: 1.859 ± 0.048	2.005AlaGln: 2.005 ± 0.051	2.579AlaArg: 2.579 ± 0.067	4.319AlaSer: 4.319 ± 0.088	3.075AlaThr: 3.075 ± 0.076	6.043AlaVal: 6.043 ± 0.113	0.516AlaTrp: 0.516 ± 0.034	2.499AlaTyr: 2.499 ± 0.075	0.0AlaXaa: 0.0 ± 0.0
Cys	0.821CysAla: 0.821 ± 0.041	0.179CysCys: 0.179 ± 0.017	0.533CysAsp: 0.533 ± 0.032	0.601CysGlu: 0.601 ± 0.029	0.58CysPhe: 0.58 ± 0.033	0.959CysGly: 0.959 ± 0.048	0.326CysHis: 0.326 ± 0.027	0.753CysIle: 0.753 ± 0.036	0.726CysLys: 0.726 ± 0.038	1.028CysLeu: 1.028 ± 0.037	0.295CysMet: 0.295 ± 0.021	0.48CysAsn: 0.48 ± 0.029	0.602CysPro: 0.602 ± 0.032	0.281CysGln: 0.281 ± 0.023	0.519CysArg: 0.519 ± 0.03	0.789CysSer: 0.789 ± 0.037	0.562CysThr: 0.562 ± 0.03	0.695CysVal: 0.695 ± 0.033	0.118CysTrp: 0.118 ± 0.015	0.422CysTyr: 0.422 ± 0.028	0.0CysXaa: 0.0 ± 0.0
Asp	3.771AspAla: 3.771 ± 0.079	0.588AspCys: 0.588 ± 0.031	3.003AspAsp: 3.003 ± 0.078	3.826AspGlu: 3.826 ± 0.071	3.006AspPhe: 3.006 ± 0.072	3.823AspGly: 3.823 ± 0.087	0.7AspHis: 0.7 ± 0.038	5.5AspIle: 5.5 ± 0.093	4.046AspLys: 4.046 ± 0.09	4.314AspLeu: 4.314 ± 0.081	1.636AspMet: 1.636 ± 0.052	2.667AspAsn: 2.667 ± 0.075	1.578AspPro: 1.578 ± 0.047	0.675AspGln: 0.675 ± 0.033	2.112AspArg: 2.112 ± 0.059	3.5AspSer: 3.5 ± 0.074	3.382AspThr: 3.382 ± 0.078	4.231AspVal: 4.231 ± 0.086	0.468AspTrp: 0.468 ± 0.027	2.229AspTyr: 2.229 ± 0.063	0.0AspXaa: 0.0 ± 0.0
Glu	4.819GluAla: 4.819 ± 0.086	0.563GluCys: 0.563 ± 0.031	3.155GluAsp: 3.155 ± 0.077	4.129GluGlu: 4.129 ± 0.093	2.664GluPhe: 2.664 ± 0.059	3.71GluGly: 3.71 ± 0.084	1.268GluHis: 1.268 ± 0.046	5.827GluIle: 5.827 ± 0.101	5.474GluLys: 5.474 ± 0.096	6.558GluLeu: 6.558 ± 0.103	1.781GluMet: 1.781 ± 0.056	3.103GluAsn: 3.103 ± 0.07	1.909GluPro: 1.909 ± 0.054	1.922GluGln: 1.922 ± 0.063	3.29GluArg: 3.29 ± 0.074	3.801GluSer: 3.801 ± 0.088	3.91GluThr: 3.91 ± 0.075	3.823GluVal: 3.823 ± 0.091	0.4GluTrp: 0.4 ± 0.027	1.754GluTyr: 1.754 ± 0.055	0.0GluXaa: 0.0 ± 0.0
Phe	2.902PheAla: 2.902 ± 0.074	0.551PheCys: 0.551 ± 0.028	2.643PheAsp: 2.643 ± 0.071	2.574PheGlu: 2.574 ± 0.07	2.554PhePhe: 2.554 ± 0.074	2.789PheGly: 2.789 ± 0.067	0.797PheHis: 0.797 ± 0.037	3.902PheIle: 3.902 ± 0.088	3.182PheLys: 3.182 ± 0.063	4.846PheLeu: 4.846 ± 0.112	1.253PheMet: 1.253 ± 0.04	2.275PheAsn: 2.275 ± 0.061	1.561PhePro: 1.561 ± 0.058	1.042PheGln: 1.042 ± 0.038	1.646PheArg: 1.646 ± 0.058	3.618PheSer: 3.618 ± 0.084	2.877PheThr: 2.877 ± 0.069	3.135PheVal: 3.135 ± 0.07	0.494PheTrp: 0.494 ± 0.032	1.917PheTyr: 1.917 ± 0.064	0.0PheXaa: 0.0 ± 0.0
Gly	4.559GlyAla: 4.559 ± 0.09	0.916GlyCys: 0.916 ± 0.048	3.279GlyAsp: 3.279 ± 0.074	3.488GlyGlu: 3.488 ± 0.073	3.371GlyPhe: 3.371 ± 0.07	4.634GlyGly: 4.634 ± 0.115	1.316GlyHis: 1.316 ± 0.046	5.646GlyIle: 5.646 ± 0.108	5.169GlyLys: 5.169 ± 0.092	6.103GlyLeu: 6.103 ± 0.105	1.948GlyMet: 1.948 ± 0.054	2.603GlyAsn: 2.603 ± 0.078	1.294GlyPro: 1.294 ± 0.051	1.708GlyGln: 1.708 ± 0.051	2.868GlyArg: 2.868 ± 0.069	3.917GlySer: 3.917 ± 0.08	3.881GlyThr: 3.881 ± 0.086	4.948GlyVal: 4.948 ± 0.082	0.587GlyTrp: 0.587 ± 0.032	2.623GlyTyr: 2.623 ± 0.078	0.0GlyXaa: 0.0 ± 0.0
His	1.072HisAla: 1.072 ± 0.042	0.265HisCys: 0.265 ± 0.023	0.963HisAsp: 0.963 ± 0.043	1.125HisGlu: 1.125 ± 0.041	0.901HisPhe: 0.901 ± 0.038	1.437HisGly: 1.437 ± 0.044	0.491HisHis: 0.491 ± 0.028	1.68HisIle: 1.68 ± 0.052	1.199HisLys: 1.199 ± 0.043	1.741HisLeu: 1.741 ± 0.05	0.431HisMet: 0.431 ± 0.026	0.825HisAsn: 0.825 ± 0.034	1.007HisPro: 1.007 ± 0.039	0.518HisGln: 0.518 ± 0.031	0.915HisArg: 0.915 ± 0.04	1.261HisSer: 1.261 ± 0.049	1.18HisThr: 1.18 ± 0.047	1.144HisVal: 1.144 ± 0.042	0.198HisTrp: 0.198 ± 0.02	0.725HisTyr: 0.725 ± 0.033	0.0HisXaa: 0.0 ± 0.0
Ile	5.951IleAla: 5.951 ± 0.101	0.853IleCys: 0.853 ± 0.039	4.39IleAsp: 4.39 ± 0.084	5.668IleGlu: 5.668 ± 0.1	3.472IlePhe: 3.472 ± 0.083	5.03IleGly: 5.03 ± 0.096	1.462IleHis: 1.462 ± 0.051	6.123IleIle: 6.123 ± 0.093	6.2IleLys: 6.2 ± 0.091	7.118IleLeu: 7.118 ± 0.118	1.963IleMet: 1.963 ± 0.061	3.944IleAsn: 3.944 ± 0.081	3.191IlePro: 3.191 ± 0.078	1.741IleGln: 1.741 ± 0.05	3.45IleArg: 3.45 ± 0.074	6.117IleSer: 6.117 ± 0.105	5.171IleThr: 5.171 ± 0.079	5.828IleVal: 5.828 ± 0.105	0.491IleTrp: 0.491 ± 0.025	2.731IleTyr: 2.731 ± 0.07	0.0IleXaa: 0.0 ± 0.0
Lys	5.052LysAla: 5.052 ± 0.094	0.675LysCys: 0.675 ± 0.034	4.722LysAsp: 4.722 ± 0.098	5.714LysGlu: 5.714 ± 0.114	2.229LysPhe: 2.229 ± 0.064	4.349LysGly: 4.349 ± 0.087	1.531LysHis: 1.531 ± 0.05	5.878LysIle: 5.878 ± 0.105	5.888LysLys: 5.888 ± 0.113	6.142LysLeu: 6.142 ± 0.101	1.906LysMet: 1.906 ± 0.059	3.558LysAsn: 3.558 ± 0.08	2.75LysPro: 2.75 ± 0.072	2.245LysGln: 2.245 ± 0.061	3.472LysArg: 3.472 ± 0.079	4.7LysSer: 4.7 ± 0.088	4.976LysThr: 4.976 ± 0.104	4.399LysVal: 4.399 ± 0.09	0.607LysTrp: 0.607 ± 0.032	2.578LysTyr: 2.578 ± 0.072	0.0LysXaa: 0.0 ± 0.0
Leu	6.183LeuAla: 6.183 ± 0.107	1.17LeuCys: 1.17 ± 0.044	4.724LeuAsp: 4.724 ± 0.086	5.314LeuGlu: 5.314 ± 0.092	4.454LeuPhe: 4.454 ± 0.094	5.304LeuGly: 5.304 ± 0.084	1.697LeuHis: 1.697 ± 0.052	7.727LeuIle: 7.727 ± 0.125	8.586LeuLys: 8.586 ± 0.116	8.908LeuLeu: 8.908 ± 0.143	2.563LeuMet: 2.563 ± 0.065	4.78LeuAsn: 4.78 ± 0.088	3.825LeuPro: 3.825 ± 0.088	2.579LeuGln: 2.579 ± 0.06	4.605LeuArg: 4.605 ± 0.078	8.142LeuSer: 8.142 ± 0.114	6.208LeuThr: 6.208 ± 0.106	5.425LeuVal: 5.425 ± 0.093	0.847LeuTrp: 0.847 ± 0.038	3.469LeuTyr: 3.469 ± 0.069	0.0LeuXaa: 0.0 ± 0.0
Met	2.221MetAla: 2.221 ± 0.061	0.29MetCys: 0.29 ± 0.023	1.603MetAsp: 1.603 ± 0.049	1.803MetGlu: 1.803 ± 0.05	1.214MetPhe: 1.214 ± 0.049	1.82MetGly: 1.82 ± 0.054	0.364MetHis: 0.364 ± 0.023	1.749MetIle: 1.749 ± 0.046	2.088MetLys: 2.088 ± 0.057	2.589MetLeu: 2.589 ± 0.071	0.714MetMet: 0.714 ± 0.03	1.009MetAsn: 1.009 ± 0.043	1.363MetPro: 1.363 ± 0.042	0.676MetGln: 0.676 ± 0.026	1.312MetArg: 1.312 ± 0.056	2.044MetSer: 2.044 ± 0.058	1.547MetThr: 1.547 ± 0.047	1.803MetVal: 1.803 ± 0.057	0.235MetTrp: 0.235 ± 0.02	0.756MetTyr: 0.756 ± 0.036	0.0MetXaa: 0.0 ± 0.0
Asn	2.927AsnAla: 2.927 ± 0.066	0.496AsnCys: 0.496 ± 0.027	2.407AsnAsp: 2.407 ± 0.065	2.587AsnGlu: 2.587 ± 0.071	1.895AsnPhe: 1.895 ± 0.055	2.76AsnGly: 2.76 ± 0.078	0.726AsnHis: 0.726 ± 0.033	4.098AsnIle: 4.098 ± 0.082	2.855AsnLys: 2.855 ± 0.067	4.328AsnLeu: 4.328 ± 0.095	1.291AsnMet: 1.291 ± 0.042	1.985AsnAsn: 1.985 ± 0.061	2.126AsnPro: 2.126 ± 0.062	0.987AsnGln: 0.987 ± 0.039	1.892AsnArg: 1.892 ± 0.047	2.918AsnSer: 2.918 ± 0.072	2.531AsnThr: 2.531 ± 0.066	3.098AsnVal: 3.098 ± 0.067	0.392AsnTrp: 0.392 ± 0.027	1.619AsnTyr: 1.619 ± 0.055	0.0AsnXaa: 0.0 ± 0.0
Pro	2.584ProAla: 2.584 ± 0.067	0.389ProCys: 0.389 ± 0.022	2.469ProAsp: 2.469 ± 0.066	2.882ProGlu: 2.882 ± 0.071	1.985ProPhe: 1.985 ± 0.058	2.055ProGly: 2.055 ± 0.067	0.849ProHis: 0.849 ± 0.039	2.494ProIle: 2.494 ± 0.065	1.997ProLys: 1.997 ± 0.063	3.568ProLeu: 3.568 ± 0.082	0.904ProMet: 0.904 ± 0.041	1.266ProAsn: 1.266 ± 0.045	1.439ProPro: 1.439 ± 0.066	1.197ProGln: 1.197 ± 0.043	1.059ProArg: 1.059 ± 0.04	2.072ProSer: 2.072 ± 0.062	1.726ProThr: 1.726 ± 0.065	3.161ProVal: 3.161 ± 0.072	0.367ProTrp: 0.367 ± 0.023	1.454ProTyr: 1.454 ± 0.048	0.0ProXaa: 0.0 ± 0.0
Gln	1.834GlnAla: 1.834 ± 0.055	0.318GlnCys: 0.318 ± 0.022	1.206GlnAsp: 1.206 ± 0.043	1.395GlnGlu: 1.395 ± 0.045	0.957GlnPhe: 0.957 ± 0.041	1.472GlnGly: 1.472 ± 0.051	0.587GlnHis: 0.587 ± 0.028	1.969GlnIle: 1.969 ± 0.056	2.098GlnLys: 2.098 ± 0.058	2.425GlnLeu: 2.425 ± 0.07	0.825GlnMet: 0.825 ± 0.031	1.21GlnAsn: 1.21 ± 0.048	0.919GlnPro: 0.919 ± 0.037	0.893GlnGln: 0.893 ± 0.039	1.515GlnArg: 1.515 ± 0.046	1.845GlnSer: 1.845 ± 0.059	1.635GlnThr: 1.635 ± 0.05	1.603GlnVal: 1.603 ± 0.051	0.3GlnTrp: 0.3 ± 0.02	0.995GlnTyr: 0.995 ± 0.038	0.0GlnXaa: 0.0 ± 0.0
Arg	2.992ArgAla: 2.992 ± 0.068	0.544ArgCys: 0.544 ± 0.033	2.416ArgAsp: 2.416 ± 0.064	2.967ArgGlu: 2.967 ± 0.068	2.143ArgPhe: 2.143 ± 0.064	2.736ArgGly: 2.736 ± 0.072	0.933ArgHis: 0.933 ± 0.041	3.213ArgIle: 3.213 ± 0.074	3.018ArgLys: 3.018 ± 0.062	4.401ArgLeu: 4.401 ± 0.09	1.15ArgMet: 1.15 ± 0.043	1.895ArgAsn: 1.895 ± 0.056	1.351ArgPro: 1.351 ± 0.046	1.439ArgGln: 1.439 ± 0.047	2.138ArgArg: 2.138 ± 0.075	2.185ArgSer: 2.185 ± 0.064	2.085ArgThr: 2.085 ± 0.054	3.076ArgVal: 3.076 ± 0.078	0.375ArgTrp: 0.375 ± 0.025	1.727ArgTyr: 1.727 ± 0.047	0.0ArgXaa: 0.0 ± 0.0
Ser	4.986SerAla: 4.986 ± 0.095	0.75SerCys: 0.75 ± 0.038	4.021SerAsp: 4.021 ± 0.076	4.366SerGlu: 4.366 ± 0.08	3.398SerPhe: 3.398 ± 0.069	5.289SerGly: 5.289 ± 0.101	1.305SerHis: 1.305 ± 0.042	5.001SerIle: 5.001 ± 0.097	4.438SerLys: 4.438 ± 0.094	6.829SerLeu: 6.829 ± 0.108	1.803SerMet: 1.803 ± 0.052	2.554SerAsn: 2.554 ± 0.069	2.349SerPro: 2.349 ± 0.074	1.771SerGln: 1.771 ± 0.052	2.579SerArg: 2.579 ± 0.067	4.601SerSer: 4.601 ± 0.102	3.513SerThr: 3.513 ± 0.076	5.325SerVal: 5.325 ± 0.102	0.596SerTrp: 0.596 ± 0.027	2.361SerTyr: 2.361 ± 0.064	0.0SerXaa: 0.0 ± 0.0
Thr	4.772ThrAla: 4.772 ± 0.104	0.585ThrCys: 0.585 ± 0.032	3.461ThrAsp: 3.461 ± 0.079	3.903ThrGlu: 3.903 ± 0.078	2.598ThrPhe: 2.598 ± 0.066	4.794ThrGly: 4.794 ± 0.079	1.224ThrHis: 1.224 ± 0.045	4.476ThrIle: 4.476 ± 0.091	3.503ThrLys: 3.503 ± 0.078	5.925ThrLeu: 5.925 ± 0.096	1.354ThrMet: 1.354 ± 0.048	2.083ThrAsn: 2.083 ± 0.071	2.524ThrPro: 2.524 ± 0.077	1.489ThrGln: 1.489 ± 0.052	1.828ThrArg: 1.828 ± 0.052	3.568ThrSer: 3.568 ± 0.079	3.2ThrThr: 3.2 ± 0.088	4.685ThrVal: 4.685 ± 0.087	0.408ThrTrp: 0.408 ± 0.025	1.821ThrTyr: 1.821 ± 0.053	0.0ThrXaa: 0.0 ± 0.0
Val	4.358ValAla: 4.358 ± 0.09	0.835ValCys: 0.835 ± 0.036	3.673ValAsp: 3.673 ± 0.075	4.253ValGlu: 4.253 ± 0.076	3.729ValPhe: 3.729 ± 0.087	3.925ValGly: 3.925 ± 0.079	1.194ValHis: 1.194 ± 0.042	5.841ValIle: 5.841 ± 0.091	5.339ValLys: 5.339 ± 0.091	7.138ValLeu: 7.138 ± 0.109	1.908ValMet: 1.908 ± 0.053	3.092ValAsn: 3.092 ± 0.068	2.52ValPro: 2.52 ± 0.066	1.625ValGln: 1.625 ± 0.052	3.025ValArg: 3.025 ± 0.072	5.569ValSer: 5.569 ± 0.085	4.435ValThr: 4.435 ± 0.081	5.362ValVal: 5.362 ± 0.119	0.551ValTrp: 0.551 ± 0.031	2.429ValTyr: 2.429 ± 0.072	0.0ValXaa: 0.0 ± 0.0
Trp	0.615TrpAla: 0.615 ± 0.03	0.118TrpCys: 0.118 ± 0.014	0.477TrpAsp: 0.477 ± 0.03	0.486TrpGlu: 0.486 ± 0.029	0.431TrpPhe: 0.431 ± 0.031	0.537TrpGly: 0.537 ± 0.036	0.198TrpHis: 0.198 ± 0.017	0.653TrpIle: 0.653 ± 0.035	0.568TrpLys: 0.568 ± 0.028	0.874TrpLeu: 0.874 ± 0.04	0.216TrpMet: 0.216 ± 0.017	0.34TrpAsn: 0.34 ± 0.019	0.265TrpPro: 0.265 ± 0.017	0.351TrpGln: 0.351 ± 0.026	0.4TrpArg: 0.4 ± 0.026	0.507TrpSer: 0.507 ± 0.028	0.405TrpThr: 0.405 ± 0.03	0.574TrpVal: 0.574 ± 0.037	0.08TrpTrp: 0.08 ± 0.011	0.287TrpTyr: 0.287 ± 0.02	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.253TyrAla: 2.253 ± 0.056	0.464TyrCys: 0.464 ± 0.029	2.148TyrAsp: 2.148 ± 0.06	2.323TyrGlu: 2.323 ± 0.075	1.771TyrPhe: 1.771 ± 0.053	2.411TyrGly: 2.411 ± 0.067	0.748TyrHis: 0.748 ± 0.035	2.574TyrIle: 2.574 ± 0.067	2.499TyrLys: 2.499 ± 0.072	3.387TyrLeu: 3.387 ± 0.086	1.043TyrMet: 1.043 ± 0.046	1.79TyrAsn: 1.79 ± 0.06	1.484TyrPro: 1.484 ± 0.051	0.857TyrGln: 0.857 ± 0.035	1.583TyrArg: 1.583 ± 0.049	2.361TyrSer: 2.361 ± 0.071	1.905TyrThr: 1.905 ± 0.056	2.441TyrVal: 2.441 ± 0.065	0.353TyrTrp: 0.353 ± 0.025	1.417TyrTyr: 1.417 ± 0.052	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2708 proteins (637417 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---