Amino acid dipepetide frequency for Bacillus phage Gxv1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.116AlaAla: 3.116 ± 0.774
1.039AlaCys: 1.039 ± 0.504
2.968AlaAsp: 2.968 ± 0.532
3.561AlaGlu: 3.561 ± 0.783
2.819AlaPhe: 2.819 ± 0.527
3.561AlaGly: 3.561 ± 0.908
1.187AlaHis: 1.187 ± 0.418
3.265AlaIle: 3.265 ± 0.781
3.561AlaLys: 3.561 ± 0.746
3.858AlaLeu: 3.858 ± 0.827
1.187AlaMet: 1.187 ± 0.389
2.226AlaAsn: 2.226 ± 0.544
1.336AlaPro: 1.336 ± 0.388
2.374AlaGln: 2.374 ± 0.675
2.671AlaArg: 2.671 ± 0.58
4.452AlaSer: 4.452 ± 0.718
2.523AlaThr: 2.523 ± 0.556
4.897AlaVal: 4.897 ± 1.126
0.89AlaTrp: 0.89 ± 0.465
3.265AlaTyr: 3.265 ± 0.61
0.0AlaXaa: 0.0 ± 0.0
Cys
0.445CysAla: 0.445 ± 0.218
0.0CysCys: 0.0 ± 0.0
0.594CysAsp: 0.594 ± 0.467
1.039CysGlu: 1.039 ± 0.388
0.297CysPhe: 0.297 ± 0.181
0.594CysGly: 0.594 ± 0.3
0.297CysHis: 0.297 ± 0.255
0.445CysIle: 0.445 ± 0.238
0.148CysLys: 0.148 ± 0.153
0.594CysLeu: 0.594 ± 0.277
0.148CysMet: 0.148 ± 0.169
0.297CysAsn: 0.297 ± 0.178
0.297CysPro: 0.297 ± 0.255
0.0CysGln: 0.0 ± 0.0
0.148CysArg: 0.148 ± 0.158
0.742CysSer: 0.742 ± 0.326
0.297CysThr: 0.297 ± 0.171
0.742CysVal: 0.742 ± 0.375
0.0CysTrp: 0.0 ± 0.0
0.89CysTyr: 0.89 ± 0.355
0.0CysXaa: 0.0 ± 0.0
Asp
2.374AspAla: 2.374 ± 0.537
0.594AspCys: 0.594 ± 0.208
4.748AspAsp: 4.748 ± 0.762
3.561AspGlu: 3.561 ± 0.525
2.968AspPhe: 2.968 ± 0.709
5.49AspGly: 5.49 ± 1.175
0.742AspHis: 0.742 ± 0.323
5.045AspIle: 5.045 ± 0.972
4.897AspLys: 4.897 ± 0.944
5.639AspLeu: 5.639 ± 0.984
1.336AspMet: 1.336 ± 0.608
5.045AspAsn: 5.045 ± 0.895
2.077AspPro: 2.077 ± 0.491
0.594AspGln: 0.594 ± 0.277
1.781AspArg: 1.781 ± 0.688
3.265AspSer: 3.265 ± 0.666
4.007AspThr: 4.007 ± 0.905
4.897AspVal: 4.897 ± 0.812
1.039AspTrp: 1.039 ± 0.47
3.413AspTyr: 3.413 ± 1.084
0.148AspXaa: 0.148 ± 0.165
Glu
3.71GluAla: 3.71 ± 1.207
0.0GluCys: 0.0 ± 0.0
4.303GluAsp: 4.303 ± 1.061
4.452GluGlu: 4.452 ± 0.858
1.632GluPhe: 1.632 ± 0.449
3.561GluGly: 3.561 ± 0.764
0.89GluHis: 0.89 ± 0.288
5.342GluIle: 5.342 ± 0.829
5.045GluLys: 5.045 ± 0.88
6.084GluLeu: 6.084 ± 0.975
1.781GluMet: 1.781 ± 0.605
4.748GluAsn: 4.748 ± 0.687
0.742GluPro: 0.742 ± 0.371
3.116GluGln: 3.116 ± 0.927
2.968GluArg: 2.968 ± 0.629
3.561GluSer: 3.561 ± 0.763
4.155GluThr: 4.155 ± 0.881
4.6GluVal: 4.6 ± 0.965
0.89GluTrp: 0.89 ± 0.536
3.561GluTyr: 3.561 ± 0.648
0.0GluXaa: 0.0 ± 0.0
Phe
2.374PheAla: 2.374 ± 0.599
0.297PheCys: 0.297 ± 0.255
3.561PheAsp: 3.561 ± 0.606
4.007PheGlu: 4.007 ± 0.726
1.336PhePhe: 1.336 ± 0.527
1.781PheGly: 1.781 ± 0.556
0.89PheHis: 0.89 ± 0.317
3.116PheIle: 3.116 ± 0.851
4.303PheLys: 4.303 ± 1.844
2.819PheLeu: 2.819 ± 0.787
1.484PheMet: 1.484 ± 0.367
3.116PheAsn: 3.116 ± 0.903
1.187PhePro: 1.187 ± 0.386
1.039PheGln: 1.039 ± 0.517
1.929PheArg: 1.929 ± 0.552
3.265PheSer: 3.265 ± 0.648
2.819PheThr: 2.819 ± 0.667
2.226PheVal: 2.226 ± 0.655
0.148PheTrp: 0.148 ± 0.163
1.781PheTyr: 1.781 ± 0.579
0.0PheXaa: 0.0 ± 0.0
Gly
3.561GlyAla: 3.561 ± 0.802
0.297GlyCys: 0.297 ± 0.268
5.49GlyAsp: 5.49 ± 1.029
3.413GlyGlu: 3.413 ± 0.655
4.007GlyPhe: 4.007 ± 1.0
6.232GlyGly: 6.232 ± 1.42
0.89GlyHis: 0.89 ± 0.41
4.155GlyIle: 4.155 ± 1.884
4.007GlyLys: 4.007 ± 0.921
5.49GlyLeu: 5.49 ± 0.71
1.632GlyMet: 1.632 ± 0.573
5.194GlyAsn: 5.194 ± 1.154
0.0GlyPro: 0.0 ± 0.0
1.929GlyGln: 1.929 ± 0.53
2.077GlyArg: 2.077 ± 0.634
4.155GlySer: 4.155 ± 0.962
4.155GlyThr: 4.155 ± 0.637
4.748GlyVal: 4.748 ± 0.941
0.742GlyTrp: 0.742 ± 0.494
3.413GlyTyr: 3.413 ± 0.814
0.0GlyXaa: 0.0 ± 0.0
His
0.594HisAla: 0.594 ± 0.266
0.0HisCys: 0.0 ± 0.0
0.89HisAsp: 0.89 ± 0.342
1.336HisGlu: 1.336 ± 0.493
0.742HisPhe: 0.742 ± 0.311
1.039HisGly: 1.039 ± 0.532
0.445HisHis: 0.445 ± 0.302
1.039HisIle: 1.039 ± 0.392
1.632HisLys: 1.632 ± 0.468
1.187HisLeu: 1.187 ± 0.475
0.445HisMet: 0.445 ± 0.205
0.742HisAsn: 0.742 ± 0.304
0.297HisPro: 0.297 ± 0.228
0.297HisGln: 0.297 ± 0.204
0.148HisArg: 0.148 ± 0.152
0.742HisSer: 0.742 ± 0.346
0.594HisThr: 0.594 ± 0.236
1.484HisVal: 1.484 ± 0.444
0.148HisTrp: 0.148 ± 0.124
1.039HisTyr: 1.039 ± 0.398
0.0HisXaa: 0.0 ± 0.0
Ile
3.71IleAla: 3.71 ± 0.619
0.594IleCys: 0.594 ± 0.304
4.452IleAsp: 4.452 ± 0.677
5.342IleGlu: 5.342 ± 1.043
2.671IlePhe: 2.671 ± 0.868
4.748IleGly: 4.748 ± 0.835
1.336IleHis: 1.336 ± 0.498
4.007IleIle: 4.007 ± 0.813
5.49IleLys: 5.49 ± 1.234
4.155IleLeu: 4.155 ± 0.945
1.336IleMet: 1.336 ± 0.599
5.936IleAsn: 5.936 ± 1.213
2.374IlePro: 2.374 ± 0.598
2.226IleGln: 2.226 ± 0.761
3.413IleArg: 3.413 ± 0.556
4.6IleSer: 4.6 ± 0.88
5.49IleThr: 5.49 ± 0.693
3.265IleVal: 3.265 ± 0.577
0.148IleTrp: 0.148 ± 0.124
2.374IleTyr: 2.374 ± 0.734
0.0IleXaa: 0.0 ± 0.0
Lys
4.007LysAla: 4.007 ± 0.854
0.297LysCys: 0.297 ± 0.204
4.6LysAsp: 4.6 ± 1.147
5.639LysGlu: 5.639 ± 1.461
3.265LysPhe: 3.265 ± 0.768
4.155LysGly: 4.155 ± 0.728
0.594LysHis: 0.594 ± 0.32
4.6LysIle: 4.6 ± 1.008
5.787LysLys: 5.787 ± 1.193
6.529LysLeu: 6.529 ± 1.015
3.858LysMet: 3.858 ± 0.726
4.155LysAsn: 4.155 ± 0.765
2.374LysPro: 2.374 ± 0.621
3.265LysGln: 3.265 ± 0.521
4.452LysArg: 4.452 ± 0.735
4.6LysSer: 4.6 ± 0.959
4.155LysThr: 4.155 ± 0.678
5.194LysVal: 5.194 ± 0.835
1.039LysTrp: 1.039 ± 0.44
1.632LysTyr: 1.632 ± 0.497
0.0LysXaa: 0.0 ± 0.0
Leu
3.858LeuAla: 3.858 ± 0.901
0.89LeuCys: 0.89 ± 0.376
4.155LeuAsp: 4.155 ± 0.604
6.084LeuGlu: 6.084 ± 1.135
3.858LeuPhe: 3.858 ± 1.006
4.155LeuGly: 4.155 ± 0.844
1.039LeuHis: 1.039 ± 0.414
4.155LeuIle: 4.155 ± 0.709
5.936LeuLys: 5.936 ± 1.116
4.897LeuLeu: 4.897 ± 0.918
2.819LeuMet: 2.819 ± 0.509
6.381LeuAsn: 6.381 ± 0.831
2.523LeuPro: 2.523 ± 0.593
2.968LeuGln: 2.968 ± 0.843
3.561LeuArg: 3.561 ± 0.88
8.013LeuSer: 8.013 ± 1.447
5.639LeuThr: 5.639 ± 0.766
4.897LeuVal: 4.897 ± 0.848
0.742LeuTrp: 0.742 ± 0.32
3.561LeuTyr: 3.561 ± 0.874
0.148LeuXaa: 0.148 ± 0.152
Met
1.187MetAla: 1.187 ± 0.43
0.297MetCys: 0.297 ± 0.202
1.187MetAsp: 1.187 ± 0.458
1.336MetGlu: 1.336 ± 0.48
0.594MetPhe: 0.594 ± 0.581
1.336MetGly: 1.336 ± 0.393
0.297MetHis: 0.297 ± 0.194
2.819MetIle: 2.819 ± 0.819
2.374MetLys: 2.374 ± 0.828
2.819MetLeu: 2.819 ± 0.733
1.187MetMet: 1.187 ± 0.45
1.632MetAsn: 1.632 ± 0.433
0.89MetPro: 0.89 ± 0.301
1.187MetGln: 1.187 ± 0.499
1.484MetArg: 1.484 ± 0.502
1.336MetSer: 1.336 ± 0.413
1.484MetThr: 1.484 ± 0.53
2.077MetVal: 2.077 ± 0.513
0.148MetTrp: 0.148 ± 0.163
1.336MetTyr: 1.336 ± 0.476
0.0MetXaa: 0.0 ± 0.0
Asn
4.6AsnAla: 4.6 ± 1.092
0.594AsnCys: 0.594 ± 0.443
4.748AsnAsp: 4.748 ± 1.152
4.155AsnGlu: 4.155 ± 0.897
1.632AsnPhe: 1.632 ± 0.408
4.452AsnGly: 4.452 ± 1.089
0.594AsnHis: 0.594 ± 0.337
4.007AsnIle: 4.007 ± 0.732
5.639AsnLys: 5.639 ± 0.925
4.303AsnLeu: 4.303 ± 0.566
2.226AsnMet: 2.226 ± 0.536
4.155AsnAsn: 4.155 ± 1.095
2.523AsnPro: 2.523 ± 0.524
3.561AsnGln: 3.561 ± 1.199
2.374AsnArg: 2.374 ± 0.59
3.858AsnSer: 3.858 ± 0.564
4.748AsnThr: 4.748 ± 0.981
5.639AsnVal: 5.639 ± 0.745
0.594AsnTrp: 0.594 ± 0.208
3.413AsnTyr: 3.413 ± 1.068
0.0AsnXaa: 0.0 ± 0.0
Pro
1.781ProAla: 1.781 ± 0.5
0.148ProCys: 0.148 ± 0.124
2.523ProAsp: 2.523 ± 0.721
1.336ProGlu: 1.336 ± 0.454
1.187ProPhe: 1.187 ± 0.443
0.445ProGly: 0.445 ± 0.218
0.445ProHis: 0.445 ± 0.282
0.89ProIle: 0.89 ± 0.311
1.336ProLys: 1.336 ± 0.509
1.929ProLeu: 1.929 ± 0.401
0.297ProMet: 0.297 ± 0.181
2.968ProAsn: 2.968 ± 0.674
0.594ProPro: 0.594 ± 0.272
1.187ProGln: 1.187 ± 0.402
1.484ProArg: 1.484 ± 0.442
1.781ProSer: 1.781 ± 0.464
2.077ProThr: 2.077 ± 0.445
2.819ProVal: 2.819 ± 0.935
0.148ProTrp: 0.148 ± 0.179
1.929ProTyr: 1.929 ± 0.371
0.148ProXaa: 0.148 ± 0.207
Gln
2.968GlnAla: 2.968 ± 0.793
0.0GlnCys: 0.0 ± 0.0
1.336GlnAsp: 1.336 ± 0.278
2.671GlnGlu: 2.671 ± 0.799
2.374GlnPhe: 2.374 ± 0.54
3.116GlnGly: 3.116 ± 0.585
0.445GlnHis: 0.445 ± 0.283
2.226GlnIle: 2.226 ± 0.608
2.374GlnLys: 2.374 ± 0.883
3.413GlnLeu: 3.413 ± 0.702
1.187GlnMet: 1.187 ± 0.429
1.336GlnAsn: 1.336 ± 0.435
0.297GlnPro: 0.297 ± 0.184
1.039GlnGln: 1.039 ± 0.411
2.226GlnArg: 2.226 ± 0.954
2.226GlnSer: 2.226 ± 0.71
1.484GlnThr: 1.484 ± 0.542
2.968GlnVal: 2.968 ± 0.622
0.594GlnTrp: 0.594 ± 0.287
1.484GlnTyr: 1.484 ± 0.464
0.148GlnXaa: 0.148 ± 0.153
Arg
2.077ArgAla: 2.077 ± 0.572
0.445ArgCys: 0.445 ± 0.256
1.632ArgAsp: 1.632 ± 0.691
3.116ArgGlu: 3.116 ± 0.801
2.077ArgPhe: 2.077 ± 1.005
3.561ArgGly: 3.561 ± 0.613
0.445ArgHis: 0.445 ± 0.308
3.413ArgIle: 3.413 ± 0.692
3.265ArgLys: 3.265 ± 0.852
4.007ArgLeu: 4.007 ± 0.761
1.336ArgMet: 1.336 ± 0.472
3.265ArgAsn: 3.265 ± 0.712
1.187ArgPro: 1.187 ± 0.302
1.039ArgGln: 1.039 ± 0.405
1.929ArgArg: 1.929 ± 0.738
3.265ArgSer: 3.265 ± 0.603
2.968ArgThr: 2.968 ± 0.868
2.226ArgVal: 2.226 ± 0.491
0.594ArgTrp: 0.594 ± 0.261
1.632ArgTyr: 1.632 ± 0.451
0.0ArgXaa: 0.0 ± 0.0
Ser
2.819SerAla: 2.819 ± 0.613
0.594SerCys: 0.594 ± 0.312
3.265SerAsp: 3.265 ± 0.797
5.045SerGlu: 5.045 ± 0.919
3.858SerPhe: 3.858 ± 0.933
5.49SerGly: 5.49 ± 1.121
1.039SerHis: 1.039 ± 0.406
4.6SerIle: 4.6 ± 0.797
3.561SerLys: 3.561 ± 0.91
6.678SerLeu: 6.678 ± 1.044
0.89SerMet: 0.89 ± 0.345
5.342SerAsn: 5.342 ± 1.083
1.781SerPro: 1.781 ± 0.436
2.523SerGln: 2.523 ± 0.547
3.71SerArg: 3.71 ± 0.722
5.342SerSer: 5.342 ± 1.209
4.007SerThr: 4.007 ± 0.801
4.897SerVal: 4.897 ± 0.823
0.297SerTrp: 0.297 ± 0.216
2.968SerTyr: 2.968 ± 0.728
0.148SerXaa: 0.148 ± 0.184
Thr
4.748ThrAla: 4.748 ± 0.658
0.445ThrCys: 0.445 ± 0.226
5.342ThrAsp: 5.342 ± 0.798
2.819ThrGlu: 2.819 ± 0.527
3.265ThrPhe: 3.265 ± 1.021
4.303ThrGly: 4.303 ± 0.815
1.039ThrHis: 1.039 ± 0.379
4.6ThrIle: 4.6 ± 0.805
4.897ThrLys: 4.897 ± 0.769
5.194ThrLeu: 5.194 ± 0.901
0.594ThrMet: 0.594 ± 0.281
2.077ThrAsn: 2.077 ± 0.469
2.523ThrPro: 2.523 ± 0.507
2.077ThrGln: 2.077 ± 0.777
1.929ThrArg: 1.929 ± 0.484
4.452ThrSer: 4.452 ± 1.251
5.936ThrThr: 5.936 ± 1.232
4.303ThrVal: 4.303 ± 1.011
1.484ThrTrp: 1.484 ± 0.337
2.077ThrTyr: 2.077 ± 0.441
0.297ThrXaa: 0.297 ± 0.316
Val
3.71ValAla: 3.71 ± 0.844
0.594ValCys: 0.594 ± 0.334
4.897ValAsp: 4.897 ± 1.144
2.968ValGlu: 2.968 ± 0.826
3.413ValPhe: 3.413 ± 1.172
3.265ValGly: 3.265 ± 0.56
0.445ValHis: 0.445 ± 0.254
5.045ValIle: 5.045 ± 0.873
4.897ValLys: 4.897 ± 0.859
5.787ValLeu: 5.787 ± 1.287
1.039ValMet: 1.039 ± 0.318
4.452ValAsn: 4.452 ± 0.762
2.523ValPro: 2.523 ± 0.587
3.116ValGln: 3.116 ± 0.547
2.523ValArg: 2.523 ± 0.572
6.529ValSer: 6.529 ± 1.417
4.6ValThr: 4.6 ± 0.874
4.452ValVal: 4.452 ± 0.745
1.336ValTrp: 1.336 ± 0.437
3.561ValTyr: 3.561 ± 0.749
0.0ValXaa: 0.0 ± 0.0
Trp
1.039TrpAla: 1.039 ± 0.785
0.0TrpCys: 0.0 ± 0.0
0.297TrpAsp: 0.297 ± 0.223
0.445TrpGlu: 0.445 ± 0.202
0.742TrpPhe: 0.742 ± 0.44
0.297TrpGly: 0.297 ± 0.17
0.445TrpHis: 0.445 ± 0.218
0.445TrpIle: 0.445 ± 0.281
1.039TrpLys: 1.039 ± 0.463
0.89TrpLeu: 0.89 ± 0.59
0.742TrpMet: 0.742 ± 0.331
1.336TrpAsn: 1.336 ± 0.425
0.0TrpPro: 0.0 ± 0.0
1.039TrpGln: 1.039 ± 0.357
0.594TrpArg: 0.594 ± 0.306
0.742TrpSer: 0.742 ± 0.271
0.742TrpThr: 0.742 ± 0.491
0.742TrpVal: 0.742 ± 0.295
0.0TrpTrp: 0.0 ± 0.0
0.445TrpTyr: 0.445 ± 0.196
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.077TyrAla: 2.077 ± 0.628
0.742TyrCys: 0.742 ± 0.374
2.523TyrAsp: 2.523 ± 0.59
2.819TyrGlu: 2.819 ± 0.584
0.742TyrPhe: 0.742 ± 0.361
4.007TyrGly: 4.007 ± 0.952
1.336TyrHis: 1.336 ± 0.469
4.303TyrIle: 4.303 ± 0.915
4.007TyrLys: 4.007 ± 0.771
4.007TyrLeu: 4.007 ± 0.597
1.336TyrMet: 1.336 ± 0.533
3.265TyrAsn: 3.265 ± 0.725
1.929TyrPro: 1.929 ± 0.565
1.039TyrGln: 1.039 ± 0.419
2.077TyrArg: 2.077 ± 0.745
1.929TyrSer: 1.929 ± 0.42
2.523TyrThr: 2.523 ± 0.748
2.077TyrVal: 2.077 ± 0.505
1.039TyrTrp: 1.039 ± 0.44
1.484TyrTyr: 1.484 ± 0.608
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.148XaaCys: 0.148 ± 0.158
0.148XaaAsp: 0.148 ± 0.207
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.148XaaIle: 0.148 ± 0.158
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.148XaaAsn: 0.148 ± 0.165
0.0XaaPro: 0.0 ± 0.0
0.297XaaGln: 0.297 ± 0.223
0.148XaaArg: 0.148 ± 0.153
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 33 proteins (6740 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski