Turnip crinkle virus (TCV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Procedovirinae; Betacarmovirus

Average proteome isoelectric point is 8.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P17461|MP1_TCV Double gene block protein 1 OS=Turnip crinkle virus OX=11988 GN=ORF2 PE=1 SV=1
MM1 pKa = 7.75PLLHH5 pKa = 6.57TLNTALAVGLLGARR19 pKa = 11.84YY20 pKa = 8.67YY21 pKa = 10.55PEE23 pKa = 3.87VQTFLGLPDD32 pKa = 3.87YY33 pKa = 10.32VGHH36 pKa = 6.28MKK38 pKa = 10.61NVVRR42 pKa = 11.84SVFQGSGLVVVSSDD56 pKa = 3.32TVGVRR61 pKa = 11.84GTYY64 pKa = 9.02SNRR67 pKa = 11.84GQIGSSLGCILAVPDD82 pKa = 3.83SGADD86 pKa = 3.32IEE88 pKa = 4.6IDD90 pKa = 3.42LDD92 pKa = 3.78RR93 pKa = 11.84LVGTEE98 pKa = 4.57EE99 pKa = 4.08EE100 pKa = 4.31ATSCLVEE107 pKa = 4.35AVGSTADD114 pKa = 3.29VPRR117 pKa = 11.84RR118 pKa = 11.84RR119 pKa = 11.84VRR121 pKa = 11.84QKK123 pKa = 10.95GRR125 pKa = 11.84FAMHH129 pKa = 6.97AVNAAKK135 pKa = 10.38LHH137 pKa = 5.72FCGVPKK143 pKa = 9.16PTEE146 pKa = 3.93ANRR149 pKa = 11.84LAVSKK154 pKa = 10.24WLVQYY159 pKa = 10.57CKK161 pKa = 9.79EE162 pKa = 3.85RR163 pKa = 11.84HH164 pKa = 5.4VVDD167 pKa = 2.94SHH169 pKa = 7.07IRR171 pKa = 11.84TIVNTALPRR180 pKa = 11.84VFTPDD185 pKa = 3.26AEE187 pKa = 5.01DD188 pKa = 3.39IQVVLDD194 pKa = 3.55LHH196 pKa = 6.24SVRR199 pKa = 11.84AHH201 pKa = 5.5DD202 pKa = 4.37HH203 pKa = 6.13RR204 pKa = 11.84NALAEE209 pKa = 4.24AGKK212 pKa = 9.78VRR214 pKa = 11.84KK215 pKa = 8.39WWVNLAMHH223 pKa = 7.07PMTGRR228 pKa = 11.84SWSRR232 pKa = 11.84AWRR235 pKa = 11.84RR236 pKa = 11.84LCRR239 pKa = 11.84LPDD242 pKa = 3.68DD243 pKa = 3.79QAISFVRR250 pKa = 11.84XGCLRR255 pKa = 11.84EE256 pKa = 3.88LVGRR260 pKa = 11.84EE261 pKa = 3.84TQISRR266 pKa = 11.84GEE268 pKa = 4.04NPAMRR273 pKa = 11.84VFPLANPPKK282 pKa = 9.64VRR284 pKa = 11.84RR285 pKa = 11.84IFHH288 pKa = 5.85ICGMGNGLDD297 pKa = 3.67FGVHH301 pKa = 5.81NNSLNNLRR309 pKa = 11.84RR310 pKa = 11.84GLMEE314 pKa = 3.52RR315 pKa = 11.84VFYY318 pKa = 11.46VEE320 pKa = 5.45DD321 pKa = 3.56AQKK324 pKa = 10.44QLKK327 pKa = 8.73PAPQPIPGIFGKK339 pKa = 10.79LSGIRR344 pKa = 11.84RR345 pKa = 11.84RR346 pKa = 11.84LVRR349 pKa = 11.84LAGNHH354 pKa = 4.98TPVPRR359 pKa = 11.84EE360 pKa = 4.13KK361 pKa = 10.99YY362 pKa = 8.25PSFYY366 pKa = 10.14KK367 pKa = 10.54GRR369 pKa = 11.84RR370 pKa = 11.84ATIYY374 pKa = 10.56QKK376 pKa = 11.35ALDD379 pKa = 4.16SLHH382 pKa = 6.9DD383 pKa = 3.83RR384 pKa = 11.84PVSRR388 pKa = 11.84KK389 pKa = 9.37DD390 pKa = 3.43AEE392 pKa = 4.12LKK394 pKa = 10.12TFVKK398 pKa = 10.54AEE400 pKa = 4.43KK401 pKa = 10.48INFTAKK407 pKa = 10.05KK408 pKa = 9.71DD409 pKa = 3.4PAPRR413 pKa = 11.84VIQPRR418 pKa = 11.84DD419 pKa = 3.13PRR421 pKa = 11.84YY422 pKa = 9.34NIEE425 pKa = 3.65VGKK428 pKa = 9.55YY429 pKa = 7.77LKK431 pKa = 10.27PYY433 pKa = 8.5EE434 pKa = 4.11HH435 pKa = 6.95HH436 pKa = 7.36LYY438 pKa = 10.4RR439 pKa = 11.84AIDD442 pKa = 3.81AMWGGPTVLKK452 pKa = 10.64GYY454 pKa = 10.56DD455 pKa = 3.27VGEE458 pKa = 4.7LGNIMSNTWDD468 pKa = 3.73KK469 pKa = 10.79FRR471 pKa = 11.84KK472 pKa = 6.87TCAIGFDD479 pKa = 3.63MKK481 pKa = 10.85RR482 pKa = 11.84FDD484 pKa = 3.31QHH486 pKa = 7.89VSVDD490 pKa = 3.41ALRR493 pKa = 11.84WEE495 pKa = 4.15HH496 pKa = 6.05SVYY499 pKa = 10.69NAGFNCPEE507 pKa = 4.32LAQLLTWQLTNKK519 pKa = 10.03GVGRR523 pKa = 11.84ASDD526 pKa = 4.1GFIKK530 pKa = 10.79YY531 pKa = 9.73QVDD534 pKa = 3.98GCRR537 pKa = 11.84MSGDD541 pKa = 3.42VNTALGNCLLACSITKK557 pKa = 10.61YY558 pKa = 10.55LMKK561 pKa = 10.64GIKK564 pKa = 9.73CKK566 pKa = 10.67LINNGDD572 pKa = 3.67DD573 pKa = 3.17CVLFFEE579 pKa = 5.06ADD581 pKa = 3.06EE582 pKa = 4.16VDD584 pKa = 3.48RR585 pKa = 11.84VRR587 pKa = 11.84EE588 pKa = 4.03RR589 pKa = 11.84LHH591 pKa = 6.74HH592 pKa = 6.35WIDD595 pKa = 4.2FGFQCIAEE603 pKa = 4.24EE604 pKa = 3.91PQYY607 pKa = 10.96EE608 pKa = 4.2LEE610 pKa = 4.43KK611 pKa = 11.27VEE613 pKa = 4.57FCQMSPIFDD622 pKa = 3.7GEE624 pKa = 4.31GWVMVRR630 pKa = 11.84NPRR633 pKa = 11.84VSLSKK638 pKa = 10.67DD639 pKa = 3.38SYY641 pKa = 11.38STTQWANEE649 pKa = 3.56KK650 pKa = 10.51DD651 pKa = 3.47AARR654 pKa = 11.84WLAAIGEE661 pKa = 4.56CGLAIAGGVPVLQSYY676 pKa = 7.5YY677 pKa = 11.16SCLKK681 pKa = 10.78RR682 pKa = 11.84NFGPLAGDD690 pKa = 3.76YY691 pKa = 9.59KK692 pKa = 11.19KK693 pKa = 11.07KK694 pKa = 9.14MQDD697 pKa = 2.83VSFDD701 pKa = 3.28SGFYY705 pKa = 10.15RR706 pKa = 11.84LSKK709 pKa = 10.85NGMRR713 pKa = 11.84GSKK716 pKa = 10.13DD717 pKa = 3.23VSQDD721 pKa = 2.78ARR723 pKa = 11.84FSFYY727 pKa = 10.6RR728 pKa = 11.84GFGYY732 pKa = 8.92TPDD735 pKa = 3.5EE736 pKa = 4.16QEE738 pKa = 4.36ALEE741 pKa = 4.36EE742 pKa = 4.46YY743 pKa = 10.85YY744 pKa = 11.21DD745 pKa = 4.28NLQLLCEE752 pKa = 4.43WDD754 pKa = 3.19PTGYY758 pKa = 10.36KK759 pKa = 10.26EE760 pKa = 4.1EE761 pKa = 4.93LSDD764 pKa = 3.76RR765 pKa = 11.84WILNEE770 pKa = 4.91FPTTLL775 pKa = 3.63

Molecular weight: 87.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q7TD19|MP2_TCV Double gene block protein 2 OS=Turnip crinkle virus OX=11988 GN=ORF3 PE=1 SV=2
MM1 pKa = 7.84DD2 pKa = 5.49PEE4 pKa = 5.01RR5 pKa = 11.84IPYY8 pKa = 10.21NSLSDD13 pKa = 3.4SDD15 pKa = 3.7ATGKK19 pKa = 10.03RR20 pKa = 11.84KK21 pKa = 10.17KK22 pKa = 10.59GGEE25 pKa = 3.67KK26 pKa = 9.78SAKK29 pKa = 8.46KK30 pKa = 10.27RR31 pKa = 11.84LVASHH36 pKa = 6.8AASSVLNKK44 pKa = 9.74KK45 pKa = 10.15RR46 pKa = 11.84NEE48 pKa = 3.88GSASHH53 pKa = 6.64GGTWVIVADD62 pKa = 3.93KK63 pKa = 11.25VEE65 pKa = 3.93VSINFNFF72 pKa = 3.74

Molecular weight: 7.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1283

72

775

320.8

35.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.716 ± 1.156

1.871 ± 0.654

5.534 ± 0.594

4.988 ± 0.501

3.741 ± 0.439

7.95 ± 0.399

2.182 ± 0.644

4.053 ± 0.548

6.469 ± 1.235

8.496 ± 1.114

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.871 ± 0.283

4.131 ± 0.682

4.91 ± 0.4

3.741 ± 0.757

6.625 ± 1.257

7.093 ± 1.843

5.612 ± 1.551

7.716 ± 0.294

2.027 ± 0.144

3.196 ± 0.458
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski