Firmicutes bacterium CAG:552

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; environmental samples

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1175 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6GWI6|R6GWI6_9FIRM Membrane protein insertase YidC OS=Firmicutes bacterium CAG:552 OX=1263029 GN=BN704_00677 PE=3 SV=1
MM1 pKa = 7.27MNGLSVLPQNEE12 pKa = 4.39NLTVPAFTEE21 pKa = 3.95LLEE24 pKa = 6.21DD25 pKa = 2.92IFGYY29 pKa = 10.6LKK31 pKa = 10.85GYY33 pKa = 6.37MHH35 pKa = 7.08CKK37 pKa = 9.44IAFICDD43 pKa = 3.16TDD45 pKa = 3.41EE46 pKa = 4.9SKK48 pKa = 10.89FAGYY52 pKa = 8.29ATFLNYY58 pKa = 10.42LDD60 pKa = 3.52IHH62 pKa = 6.94VNTDD66 pKa = 2.02IRR68 pKa = 11.84TPFAHH73 pKa = 5.69SVMYY77 pKa = 10.38RR78 pKa = 11.84ISPLIEE84 pKa = 4.47ANTPNDD90 pKa = 3.9MEE92 pKa = 4.46IMILFDD98 pKa = 3.07SHH100 pKa = 7.21YY101 pKa = 11.39ADD103 pKa = 5.34AYY105 pKa = 11.47NDD107 pKa = 3.7AQNADD112 pKa = 3.08VTYY115 pKa = 10.57NLNDD119 pKa = 3.47RR120 pKa = 11.84TSYY123 pKa = 11.12QIMSEE128 pKa = 3.53IMYY131 pKa = 10.35VLEE134 pKa = 4.87DD135 pKa = 3.39MCNAQSSSLNDD146 pKa = 2.94IAQACRR152 pKa = 11.84VNYY155 pKa = 9.71NVRR158 pKa = 11.84LYY160 pKa = 10.52SVSPGGIFKK169 pKa = 10.69NLEE172 pKa = 3.89NYY174 pKa = 7.28TTISMTDD181 pKa = 2.7AVYY184 pKa = 10.19NAKK187 pKa = 10.07EE188 pKa = 3.79LVYY191 pKa = 10.78IMRR194 pKa = 11.84PDD196 pKa = 3.34ANLNLNCRR204 pKa = 11.84LLEE207 pKa = 4.16DD208 pKa = 5.46LICSFTPDD216 pKa = 3.29DD217 pKa = 4.22FYY219 pKa = 11.55AYY221 pKa = 10.46SFGSVSVPAPEE232 pKa = 4.85ASLLTIYY239 pKa = 10.5EE240 pKa = 3.92LVYY243 pKa = 10.2TYY245 pKa = 9.47EE246 pKa = 3.9EE247 pKa = 4.9CFTRR251 pKa = 11.84RR252 pKa = 11.84VEE254 pKa = 4.16AMIDD258 pKa = 3.64FLEE261 pKa = 6.04DD262 pKa = 3.55EE263 pKa = 5.22DD264 pKa = 6.02LSDD267 pKa = 3.53YY268 pKa = 10.92NNWYY272 pKa = 9.28GVCDD276 pKa = 3.67ISYY279 pKa = 9.92KK280 pKa = 10.45ILGTGPDD287 pKa = 2.92GWMISVLGYY296 pKa = 9.99GSKK299 pKa = 10.5VKK301 pKa = 10.09PLYY304 pKa = 10.45DD305 pKa = 3.18

Molecular weight: 34.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6GXY9|R6GXY9_9FIRM Phosphomannomutase OS=Firmicutes bacterium CAG:552 OX=1263029 GN=BN704_00880 PE=3 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.7QPKK8 pKa = 9.37KK9 pKa = 8.53RR10 pKa = 11.84ARR12 pKa = 11.84QKK14 pKa = 9.77VHH16 pKa = 6.35GFRR19 pKa = 11.84QRR21 pKa = 11.84MKK23 pKa = 7.98TQGGRR28 pKa = 11.84RR29 pKa = 11.84VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.15GRR39 pKa = 11.84KK40 pKa = 8.25NISVKK45 pKa = 10.49PLGSHH50 pKa = 6.88

Molecular weight: 6.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1175

0

1175

378033

30

2861

321.7

35.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.987 ± 0.061

1.808 ± 0.036

5.97 ± 0.066

6.548 ± 0.071

4.372 ± 0.052

7.113 ± 0.06

1.277 ± 0.026

7.609 ± 0.055

7.66 ± 0.061

8.629 ± 0.076

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.547 ± 0.037

4.504 ± 0.053

3.015 ± 0.03

2.285 ± 0.031

4.136 ± 0.06

6.693 ± 0.07

5.428 ± 0.076

7.802 ± 0.068

0.565 ± 0.019

4.053 ± 0.057
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski