Amino acid dipepetide frequency for Synechococcus phage S-EIVl

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.027AlaAla: 9.027 ± 0.995
0.864AlaCys: 0.864 ± 0.253
4.708AlaAsp: 4.708 ± 0.489
4.017AlaGlu: 4.017 ± 0.472
2.548AlaPhe: 2.548 ± 0.354
7.558AlaGly: 7.558 ± 0.956
1.08AlaHis: 1.08 ± 0.246
3.887AlaIle: 3.887 ± 0.383
4.535AlaLys: 4.535 ± 0.488
6.911AlaLeu: 6.911 ± 0.668
2.332AlaMet: 2.332 ± 0.335
3.585AlaAsn: 3.585 ± 0.439
3.714AlaPro: 3.714 ± 0.437
4.578AlaGln: 4.578 ± 0.522
4.103AlaArg: 4.103 ± 0.477
5.226AlaSer: 5.226 ± 0.613
5.658AlaThr: 5.658 ± 0.606
3.974AlaVal: 3.974 ± 0.449
1.598AlaTrp: 1.598 ± 0.277
3.023AlaTyr: 3.023 ± 0.387
0.0AlaXaa: 0.0 ± 0.0
Cys
0.561CysAla: 0.561 ± 0.127
0.13CysCys: 0.13 ± 0.068
0.734CysAsp: 0.734 ± 0.199
0.691CysGlu: 0.691 ± 0.168
0.389CysPhe: 0.389 ± 0.13
0.777CysGly: 0.777 ± 0.168
0.302CysHis: 0.302 ± 0.115
0.518CysIle: 0.518 ± 0.136
0.821CysLys: 0.821 ± 0.196
0.907CysLeu: 0.907 ± 0.288
0.216CysMet: 0.216 ± 0.083
0.605CysAsn: 0.605 ± 0.14
0.475CysPro: 0.475 ± 0.178
0.346CysGln: 0.346 ± 0.135
0.518CysArg: 0.518 ± 0.176
0.734CysSer: 0.734 ± 0.202
0.389CysThr: 0.389 ± 0.133
0.777CysVal: 0.777 ± 0.167
0.173CysTrp: 0.173 ± 0.082
0.302CysTyr: 0.302 ± 0.122
0.0CysXaa: 0.0 ± 0.0
Asp
4.06AspAla: 4.06 ± 0.382
0.432AspCys: 0.432 ± 0.117
2.98AspAsp: 2.98 ± 0.453
4.535AspGlu: 4.535 ± 0.695
2.678AspPhe: 2.678 ± 0.402
3.369AspGly: 3.369 ± 0.546
0.907AspHis: 0.907 ± 0.212
3.801AspIle: 3.801 ± 0.377
3.11AspLys: 3.11 ± 0.433
5.183AspLeu: 5.183 ± 0.523
1.728AspMet: 1.728 ± 0.289
2.16AspAsn: 2.16 ± 0.298
3.671AspPro: 3.671 ± 0.382
2.894AspGln: 2.894 ± 0.472
2.548AspArg: 2.548 ± 0.338
3.283AspSer: 3.283 ± 0.5
3.628AspThr: 3.628 ± 0.566
3.023AspVal: 3.023 ± 0.462
1.296AspTrp: 1.296 ± 0.304
2.894AspTyr: 2.894 ± 0.309
0.0AspXaa: 0.0 ± 0.0
Glu
4.492GluAla: 4.492 ± 0.439
0.691GluCys: 0.691 ± 0.192
4.319GluAsp: 4.319 ± 0.501
4.578GluGlu: 4.578 ± 0.547
2.764GluPhe: 2.764 ± 0.4
3.844GluGly: 3.844 ± 0.4
1.339GluHis: 1.339 ± 0.236
3.412GluIle: 3.412 ± 0.394
3.628GluLys: 3.628 ± 0.44
5.788GluLeu: 5.788 ± 0.714
2.376GluMet: 2.376 ± 0.411
2.807GluAsn: 2.807 ± 0.45
2.548GluPro: 2.548 ± 0.472
3.887GluGln: 3.887 ± 0.502
3.714GluArg: 3.714 ± 0.489
3.498GluSer: 3.498 ± 0.417
4.017GluThr: 4.017 ± 0.387
2.807GluVal: 2.807 ± 0.32
0.95GluTrp: 0.95 ± 0.219
2.246GluTyr: 2.246 ± 0.327
0.0GluXaa: 0.0 ± 0.0
Phe
2.851PheAla: 2.851 ± 0.4
0.518PheCys: 0.518 ± 0.126
2.376PheAsp: 2.376 ± 0.389
1.512PheGlu: 1.512 ± 0.301
1.512PhePhe: 1.512 ± 0.233
3.196PheGly: 3.196 ± 0.419
0.259PheHis: 0.259 ± 0.132
1.987PheIle: 1.987 ± 0.322
2.246PheLys: 2.246 ± 0.302
2.462PheLeu: 2.462 ± 0.387
1.123PheMet: 1.123 ± 0.229
2.073PheAsn: 2.073 ± 0.281
1.944PhePro: 1.944 ± 0.335
2.203PheGln: 2.203 ± 0.294
1.814PheArg: 1.814 ± 0.282
2.462PheSer: 2.462 ± 0.349
3.283PheThr: 3.283 ± 0.769
1.814PheVal: 1.814 ± 0.25
0.605PheTrp: 0.605 ± 0.17
1.555PheTyr: 1.555 ± 0.292
0.0PheXaa: 0.0 ± 0.0
Gly
6.306GlyAla: 6.306 ± 0.952
0.821GlyCys: 0.821 ± 0.222
3.498GlyAsp: 3.498 ± 0.35
4.017GlyGlu: 4.017 ± 0.457
3.326GlyPhe: 3.326 ± 0.403
7.083GlyGly: 7.083 ± 1.034
0.777GlyHis: 0.777 ± 0.202
4.924GlyIle: 4.924 ± 0.426
4.405GlyLys: 4.405 ± 0.591
5.874GlyLeu: 5.874 ± 0.541
1.814GlyMet: 1.814 ± 0.265
4.449GlyAsn: 4.449 ± 0.64
2.764GlyPro: 2.764 ± 0.409
3.196GlyGln: 3.196 ± 0.385
4.19GlyArg: 4.19 ± 0.483
6.435GlySer: 6.435 ± 0.663
6.565GlyThr: 6.565 ± 1.497
6.004GlyVal: 6.004 ± 0.824
1.253GlyTrp: 1.253 ± 0.249
3.455GlyTyr: 3.455 ± 0.477
0.0GlyXaa: 0.0 ± 0.0
His
1.253HisAla: 1.253 ± 0.242
0.216HisCys: 0.216 ± 0.097
0.864HisAsp: 0.864 ± 0.206
0.993HisGlu: 0.993 ± 0.184
0.259HisPhe: 0.259 ± 0.102
1.037HisGly: 1.037 ± 0.259
0.475HisHis: 0.475 ± 0.165
0.993HisIle: 0.993 ± 0.208
0.777HisLys: 0.777 ± 0.183
1.684HisLeu: 1.684 ± 0.317
0.346HisMet: 0.346 ± 0.122
0.777HisAsn: 0.777 ± 0.177
0.734HisPro: 0.734 ± 0.223
0.691HisGln: 0.691 ± 0.179
0.605HisArg: 0.605 ± 0.176
0.777HisSer: 0.777 ± 0.179
0.777HisThr: 0.777 ± 0.193
0.691HisVal: 0.691 ± 0.213
0.302HisTrp: 0.302 ± 0.105
0.432HisTyr: 0.432 ± 0.131
0.0HisXaa: 0.0 ± 0.0
Ile
5.053IleAla: 5.053 ± 0.705
0.734IleCys: 0.734 ± 0.173
4.19IleAsp: 4.19 ± 0.447
3.628IleGlu: 3.628 ± 0.469
1.468IlePhe: 1.468 ± 0.243
2.721IleGly: 2.721 ± 0.34
0.821IleHis: 0.821 ± 0.215
2.289IleIle: 2.289 ± 0.329
2.591IleLys: 2.591 ± 0.31
4.233IleLeu: 4.233 ± 0.41
0.907IleMet: 0.907 ± 0.218
2.937IleAsn: 2.937 ± 0.371
3.196IlePro: 3.196 ± 0.4
2.246IleGln: 2.246 ± 0.256
2.635IleArg: 2.635 ± 0.351
3.844IleSer: 3.844 ± 0.356
3.455IleThr: 3.455 ± 0.557
3.153IleVal: 3.153 ± 0.394
0.605IleTrp: 0.605 ± 0.155
1.598IleTyr: 1.598 ± 0.242
0.0IleXaa: 0.0 ± 0.0
Lys
4.621LysAla: 4.621 ± 0.528
0.346LysCys: 0.346 ± 0.12
2.894LysAsp: 2.894 ± 0.377
5.097LysGlu: 5.097 ± 0.448
1.857LysPhe: 1.857 ± 0.306
4.708LysGly: 4.708 ± 0.468
0.864LysHis: 0.864 ± 0.199
2.376LysIle: 2.376 ± 0.29
5.226LysLys: 5.226 ± 0.744
4.017LysLeu: 4.017 ± 0.425
1.555LysMet: 1.555 ± 0.295
2.246LysAsn: 2.246 ± 0.392
2.289LysPro: 2.289 ± 0.295
2.894LysGln: 2.894 ± 0.329
3.283LysArg: 3.283 ± 0.423
2.98LysSer: 2.98 ± 0.425
3.671LysThr: 3.671 ± 0.504
2.635LysVal: 2.635 ± 0.391
1.08LysTrp: 1.08 ± 0.209
2.635LysTyr: 2.635 ± 0.367
0.0LysXaa: 0.0 ± 0.0
Leu
6.824LeuAla: 6.824 ± 0.519
0.821LeuCys: 0.821 ± 0.216
5.356LeuAsp: 5.356 ± 0.492
5.788LeuGlu: 5.788 ± 0.531
2.894LeuPhe: 2.894 ± 0.351
6.738LeuGly: 6.738 ± 0.621
1.296LeuHis: 1.296 ± 0.251
4.19LeuIle: 4.19 ± 0.485
4.837LeuLys: 4.837 ± 0.477
6.565LeuLeu: 6.565 ± 0.666
1.771LeuMet: 1.771 ± 0.346
4.146LeuAsn: 4.146 ± 0.405
3.628LeuPro: 3.628 ± 0.423
3.326LeuGln: 3.326 ± 0.387
3.758LeuArg: 3.758 ± 0.36
5.701LeuSer: 5.701 ± 0.562
6.306LeuThr: 6.306 ± 0.652
4.19LeuVal: 4.19 ± 0.355
0.734LeuTrp: 0.734 ± 0.182
2.635LeuTyr: 2.635 ± 0.314
0.0LeuXaa: 0.0 ± 0.0
Met
2.289MetAla: 2.289 ± 0.382
0.173MetCys: 0.173 ± 0.095
1.512MetAsp: 1.512 ± 0.245
1.512MetGlu: 1.512 ± 0.306
0.95MetPhe: 0.95 ± 0.191
2.116MetGly: 2.116 ± 0.368
0.648MetHis: 0.648 ± 0.172
1.166MetIle: 1.166 ± 0.209
1.468MetLys: 1.468 ± 0.264
1.425MetLeu: 1.425 ± 0.196
0.734MetMet: 0.734 ± 0.202
0.821MetAsn: 0.821 ± 0.201
1.382MetPro: 1.382 ± 0.241
1.08MetGln: 1.08 ± 0.272
1.468MetArg: 1.468 ± 0.263
1.857MetSer: 1.857 ± 0.281
2.203MetThr: 2.203 ± 0.341
1.382MetVal: 1.382 ± 0.208
0.173MetTrp: 0.173 ± 0.088
0.648MetTyr: 0.648 ± 0.158
0.0MetXaa: 0.0 ± 0.0
Asn
3.542AsnAla: 3.542 ± 0.388
0.475AsnCys: 0.475 ± 0.118
2.678AsnAsp: 2.678 ± 0.342
2.635AsnGlu: 2.635 ± 0.346
2.203AsnPhe: 2.203 ± 0.357
2.635AsnGly: 2.635 ± 0.336
0.821AsnHis: 0.821 ± 0.233
2.678AsnIle: 2.678 ± 0.42
2.635AsnLys: 2.635 ± 0.339
4.319AsnLeu: 4.319 ± 0.463
0.993AsnMet: 0.993 ± 0.19
2.332AsnAsn: 2.332 ± 0.292
3.023AsnPro: 3.023 ± 0.386
2.462AsnGln: 2.462 ± 0.349
2.203AsnArg: 2.203 ± 0.278
2.246AsnSer: 2.246 ± 0.353
2.894AsnThr: 2.894 ± 0.435
2.462AsnVal: 2.462 ± 0.322
0.821AsnTrp: 0.821 ± 0.17
1.987AsnTyr: 1.987 ± 0.345
0.0AsnXaa: 0.0 ± 0.0
Pro
4.146ProAla: 4.146 ± 0.438
0.346ProCys: 0.346 ± 0.114
3.758ProAsp: 3.758 ± 0.475
3.369ProGlu: 3.369 ± 0.411
1.728ProPhe: 1.728 ± 0.25
4.535ProGly: 4.535 ± 0.594
0.475ProHis: 0.475 ± 0.138
2.635ProIle: 2.635 ± 0.345
2.332ProLys: 2.332 ± 0.32
3.412ProLeu: 3.412 ± 0.447
0.734ProMet: 0.734 ± 0.156
1.987ProAsn: 1.987 ± 0.309
2.591ProPro: 2.591 ± 0.427
2.332ProGln: 2.332 ± 0.404
1.814ProArg: 1.814 ± 0.296
3.801ProSer: 3.801 ± 0.493
3.542ProThr: 3.542 ± 0.357
2.98ProVal: 2.98 ± 0.306
0.389ProTrp: 0.389 ± 0.119
1.555ProTyr: 1.555 ± 0.253
0.0ProXaa: 0.0 ± 0.0
Gln
3.758GlnAla: 3.758 ± 0.529
0.432GlnCys: 0.432 ± 0.138
2.635GlnAsp: 2.635 ± 0.36
3.239GlnGlu: 3.239 ± 0.425
1.987GlnPhe: 1.987 ± 0.284
4.405GlnGly: 4.405 ± 0.454
0.648GlnHis: 0.648 ± 0.162
2.591GlnIle: 2.591 ± 0.329
2.764GlnLys: 2.764 ± 0.347
4.405GlnLeu: 4.405 ± 0.59
1.037GlnMet: 1.037 ± 0.233
1.944GlnAsn: 1.944 ± 0.245
2.376GlnPro: 2.376 ± 0.384
3.498GlnGln: 3.498 ± 0.51
2.505GlnArg: 2.505 ± 0.343
2.376GlnSer: 2.376 ± 0.293
2.894GlnThr: 2.894 ± 0.378
3.585GlnVal: 3.585 ± 0.359
0.648GlnTrp: 0.648 ± 0.153
0.993GlnTyr: 0.993 ± 0.187
0.0GlnXaa: 0.0 ± 0.0
Arg
3.758ArgAla: 3.758 ± 0.456
0.864ArgCys: 0.864 ± 0.193
2.246ArgAsp: 2.246 ± 0.304
3.196ArgGlu: 3.196 ± 0.371
2.246ArgPhe: 2.246 ± 0.326
3.369ArgGly: 3.369 ± 0.48
0.605ArgHis: 0.605 ± 0.17
2.635ArgIle: 2.635 ± 0.389
2.764ArgLys: 2.764 ± 0.402
4.621ArgLeu: 4.621 ± 0.49
1.166ArgMet: 1.166 ± 0.206
2.505ArgAsn: 2.505 ± 0.276
2.16ArgPro: 2.16 ± 0.377
2.462ArgGln: 2.462 ± 0.417
2.764ArgArg: 2.764 ± 0.359
2.764ArgSer: 2.764 ± 0.322
2.851ArgThr: 2.851 ± 0.408
2.894ArgVal: 2.894 ± 0.403
0.605ArgTrp: 0.605 ± 0.161
1.987ArgTyr: 1.987 ± 0.311
0.0ArgXaa: 0.0 ± 0.0
Ser
5.14SerAla: 5.14 ± 0.5
0.389SerCys: 0.389 ± 0.128
2.894SerAsp: 2.894 ± 0.295
3.628SerGlu: 3.628 ± 0.329
3.11SerPhe: 3.11 ± 0.322
7.213SerGly: 7.213 ± 1.309
0.734SerHis: 0.734 ± 0.248
3.067SerIle: 3.067 ± 0.352
3.758SerLys: 3.758 ± 0.446
5.312SerLeu: 5.312 ± 0.457
1.684SerMet: 1.684 ± 0.246
3.196SerAsn: 3.196 ± 0.352
3.196SerPro: 3.196 ± 0.418
2.16SerGln: 2.16 ± 0.341
2.635SerArg: 2.635 ± 0.365
4.233SerSer: 4.233 ± 0.54
4.19SerThr: 4.19 ± 0.428
5.14SerVal: 5.14 ± 0.478
0.648SerTrp: 0.648 ± 0.141
2.246SerTyr: 2.246 ± 0.356
0.0SerXaa: 0.0 ± 0.0
Thr
6.047ThrAla: 6.047 ± 0.783
0.648ThrCys: 0.648 ± 0.152
3.542ThrAsp: 3.542 ± 0.421
3.714ThrGlu: 3.714 ± 0.371
2.419ThrPhe: 2.419 ± 0.523
7.256ThrGly: 7.256 ± 1.923
0.864ThrHis: 0.864 ± 0.232
4.708ThrIle: 4.708 ± 0.569
3.585ThrLys: 3.585 ± 0.449
5.183ThrLeu: 5.183 ± 0.469
1.641ThrMet: 1.641 ± 0.263
2.678ThrAsn: 2.678 ± 0.396
3.585ThrPro: 3.585 ± 0.435
3.671ThrGln: 3.671 ± 0.412
2.376ThrArg: 2.376 ± 0.351
5.485ThrSer: 5.485 ± 0.836
6.695ThrThr: 6.695 ± 2.17
4.146ThrVal: 4.146 ± 0.725
0.821ThrTrp: 0.821 ± 0.23
2.203ThrTyr: 2.203 ± 0.351
0.0ThrXaa: 0.0 ± 0.0
Val
5.14ValAla: 5.14 ± 0.473
0.561ValCys: 0.561 ± 0.161
3.887ValAsp: 3.887 ± 0.354
4.319ValGlu: 4.319 ± 0.563
1.857ValPhe: 1.857 ± 0.355
4.535ValGly: 4.535 ± 0.432
0.777ValHis: 0.777 ± 0.157
2.203ValIle: 2.203 ± 0.326
3.239ValLys: 3.239 ± 0.369
4.621ValLeu: 4.621 ± 0.512
1.166ValMet: 1.166 ± 0.228
2.246ValAsn: 2.246 ± 0.294
3.455ValPro: 3.455 ± 0.38
2.246ValGln: 2.246 ± 0.326
2.721ValArg: 2.721 ± 0.372
3.93ValSer: 3.93 ± 0.515
4.924ValThr: 4.924 ± 1.15
3.585ValVal: 3.585 ± 0.4
0.561ValTrp: 0.561 ± 0.156
1.857ValTyr: 1.857 ± 0.334
0.0ValXaa: 0.0 ± 0.0
Trp
1.08TrpAla: 1.08 ± 0.208
0.216TrpCys: 0.216 ± 0.113
1.166TrpAsp: 1.166 ± 0.295
0.907TrpGlu: 0.907 ± 0.204
0.432TrpPhe: 0.432 ± 0.144
0.95TrpGly: 0.95 ± 0.23
0.561TrpHis: 0.561 ± 0.154
0.734TrpIle: 0.734 ± 0.161
0.691TrpLys: 0.691 ± 0.17
0.993TrpLeu: 0.993 ± 0.24
0.475TrpMet: 0.475 ± 0.135
0.821TrpAsn: 0.821 ± 0.172
0.302TrpPro: 0.302 ± 0.112
0.734TrpGln: 0.734 ± 0.173
0.777TrpArg: 0.777 ± 0.177
0.777TrpSer: 0.777 ± 0.219
0.821TrpThr: 0.821 ± 0.184
0.691TrpVal: 0.691 ± 0.181
0.086TrpTrp: 0.086 ± 0.064
0.648TrpTyr: 0.648 ± 0.144
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.98TyrAla: 2.98 ± 0.405
0.691TyrCys: 0.691 ± 0.201
1.944TyrAsp: 1.944 ± 0.294
2.332TyrGlu: 2.332 ± 0.342
1.166TyrPhe: 1.166 ± 0.275
3.153TyrGly: 3.153 ± 0.453
0.432TyrHis: 0.432 ± 0.133
1.512TyrIle: 1.512 ± 0.258
1.857TyrLys: 1.857 ± 0.296
3.585TyrLeu: 3.585 ± 0.399
1.166TyrMet: 1.166 ± 0.205
1.728TyrAsn: 1.728 ± 0.267
1.425TyrPro: 1.425 ± 0.316
1.814TyrGln: 1.814 ± 0.264
2.03TyrArg: 2.03 ± 0.332
2.16TyrSer: 2.16 ± 0.286
2.505TyrThr: 2.505 ± 0.282
1.9TyrVal: 1.9 ± 0.397
0.518TyrTrp: 0.518 ± 0.15
0.993TyrTyr: 0.993 ± 0.202
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 129 proteins (23154 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski