Amino acid dipepetide frequency for Acidovorax sp. Leaf160

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	21.57AlaAla: 21.57 ± 0.219	1.364AlaCys: 1.364 ± 0.032	7.0AlaAsp: 7.0 ± 0.08	7.142AlaGlu: 7.142 ± 0.079	4.164AlaPhe: 4.164 ± 0.06	12.382AlaGly: 12.382 ± 0.107	2.961AlaHis: 2.961 ± 0.05	4.95AlaIle: 4.95 ± 0.074	3.323AlaLys: 3.323 ± 0.077	16.248AlaLeu: 16.248 ± 0.159	3.447AlaMet: 3.447 ± 0.053	2.544AlaAsn: 2.544 ± 0.052	7.651AlaPro: 7.651 ± 0.106	6.701AlaGln: 6.701 ± 0.091	10.122AlaArg: 10.122 ± 0.108	7.055AlaSer: 7.055 ± 0.084	6.661AlaThr: 6.661 ± 0.076	9.835AlaVal: 9.835 ± 0.102	2.134AlaTrp: 2.134 ± 0.043	2.421AlaTyr: 2.421 ± 0.041	0.002AlaXaa: 0.002 ± 0.001
Cys	1.14CysAla: 1.14 ± 0.032	0.108CysCys: 0.108 ± 0.01	0.511CysAsp: 0.511 ± 0.02	0.476CysGlu: 0.476 ± 0.019	0.269CysPhe: 0.269 ± 0.015	0.918CysGly: 0.918 ± 0.027	0.25CysHis: 0.25 ± 0.014	0.385CysIle: 0.385 ± 0.017	0.181CysLys: 0.181 ± 0.012	0.822CysLeu: 0.822 ± 0.023	0.22CysMet: 0.22 ± 0.012	0.221CysAsn: 0.221 ± 0.011	0.447CysPro: 0.447 ± 0.02	0.271CysGln: 0.271 ± 0.016	0.544CysArg: 0.544 ± 0.021	0.475CysSer: 0.475 ± 0.017	0.484CysThr: 0.484 ± 0.019	0.707CysVal: 0.707 ± 0.027	0.13CysTrp: 0.13 ± 0.01	0.177CysTyr: 0.177 ± 0.013	0.0CysXaa: 0.0 ± 0.0
Asp	7.659AspAla: 7.659 ± 0.09	0.444AspCys: 0.444 ± 0.017	2.571AspAsp: 2.571 ± 0.048	2.851AspGlu: 2.851 ± 0.056	1.944AspPhe: 1.944 ± 0.039	4.586AspGly: 4.586 ± 0.055	1.082AspHis: 1.082 ± 0.032	2.234AspIle: 2.234 ± 0.044	1.493AspLys: 1.493 ± 0.037	5.344AspLeu: 5.344 ± 0.062	1.2AspMet: 1.2 ± 0.035	1.111AspAsn: 1.111 ± 0.029	2.793AspPro: 2.793 ± 0.048	1.613AspGln: 1.613 ± 0.037	3.515AspArg: 3.515 ± 0.052	2.164AspSer: 2.164 ± 0.041	2.527AspThr: 2.527 ± 0.045	4.091AspVal: 4.091 ± 0.066	0.978AspTrp: 0.978 ± 0.028	1.317AspTyr: 1.317 ± 0.034	0.0AspXaa: 0.0 ± 0.0
Glu	6.867GluAla: 6.867 ± 0.086	0.364GluCys: 0.364 ± 0.017	2.165GluAsp: 2.165 ± 0.041	2.355GluGlu: 2.355 ± 0.047	1.596GluPhe: 1.596 ± 0.041	3.968GluGly: 3.968 ± 0.069	1.232GluHis: 1.232 ± 0.03	2.302GluIle: 2.302 ± 0.048	1.564GluLys: 1.564 ± 0.044	5.257GluLeu: 5.257 ± 0.072	1.179GluMet: 1.179 ± 0.029	1.099GluAsn: 1.099 ± 0.031	2.609GluPro: 2.609 ± 0.051	2.481GluGln: 2.481 ± 0.051	4.738GluArg: 4.738 ± 0.063	2.455GluSer: 2.455 ± 0.043	2.301GluThr: 2.301 ± 0.037	3.885GluVal: 3.885 ± 0.055	0.711GluTrp: 0.711 ± 0.026	0.952GluTyr: 0.952 ± 0.03	0.001GluXaa: 0.001 ± 0.001
Phe	4.267PheAla: 4.267 ± 0.061	0.384PheCys: 0.384 ± 0.018	2.328PheAsp: 2.328 ± 0.045	1.806PheGlu: 1.806 ± 0.041	1.259PhePhe: 1.259 ± 0.038	3.194PheGly: 3.194 ± 0.047	0.754PheHis: 0.754 ± 0.025	1.382PheIle: 1.382 ± 0.039	1.048PheLys: 1.048 ± 0.033	2.803PheLeu: 2.803 ± 0.051	0.745PheMet: 0.745 ± 0.028	1.004PheAsn: 1.004 ± 0.026	1.447PhePro: 1.447 ± 0.034	1.12PheGln: 1.12 ± 0.029	1.692PheArg: 1.692 ± 0.034	1.952PheSer: 1.952 ± 0.04	1.947PheThr: 1.947 ± 0.039	2.626PheVal: 2.626 ± 0.042	0.518PheTrp: 0.518 ± 0.022	0.804PheTyr: 0.804 ± 0.027	0.0PheXaa: 0.0 ± 0.0
Gly	10.413GlyAla: 10.413 ± 0.106	0.858GlyCys: 0.858 ± 0.024	4.216GlyAsp: 4.216 ± 0.057	4.294GlyGlu: 4.294 ± 0.067	3.221GlyPhe: 3.221 ± 0.048	7.856GlyGly: 7.856 ± 0.13	2.03GlyHis: 2.03 ± 0.05	3.695GlyIle: 3.695 ± 0.064	2.748GlyLys: 2.748 ± 0.061	9.255GlyLeu: 9.255 ± 0.088	2.417GlyMet: 2.417 ± 0.044	2.07GlyAsn: 2.07 ± 0.048	3.368GlyPro: 3.368 ± 0.053	3.643GlyGln: 3.643 ± 0.053	6.033GlyArg: 6.033 ± 0.08	4.832GlySer: 4.832 ± 0.071	4.891GlyThr: 4.891 ± 0.064	6.831GlyVal: 6.831 ± 0.077	1.601GlyTrp: 1.601 ± 0.043	2.236GlyTyr: 2.236 ± 0.039	0.002GlyXaa: 0.002 ± 0.001
His	3.325HisAla: 3.325 ± 0.063	0.281HisCys: 0.281 ± 0.015	1.221HisAsp: 1.221 ± 0.034	1.019HisGlu: 1.019 ± 0.029	0.848HisPhe: 0.848 ± 0.022	2.225HisGly: 2.225 ± 0.048	0.614HisHis: 0.614 ± 0.025	0.968HisIle: 0.968 ± 0.026	0.496HisLys: 0.496 ± 0.018	2.252HisLeu: 2.252 ± 0.048	0.46HisMet: 0.46 ± 0.018	0.431HisAsn: 0.431 ± 0.019	1.564HisPro: 1.564 ± 0.037	0.8HisGln: 0.8 ± 0.024	1.588HisArg: 1.588 ± 0.041	0.981HisSer: 0.981 ± 0.026	1.118HisThr: 1.118 ± 0.03	1.59HisVal: 1.59 ± 0.035	0.411HisTrp: 0.411 ± 0.018	0.607HisTyr: 0.607 ± 0.023	0.001HisXaa: 0.001 ± 0.001
Ile	6.125IleAla: 6.125 ± 0.08	0.371IleCys: 0.371 ± 0.015	2.985IleAsp: 2.985 ± 0.044	2.628IleGlu: 2.628 ± 0.056	1.157IlePhe: 1.157 ± 0.031	3.825IleGly: 3.825 ± 0.06	0.779IleHis: 0.779 ± 0.026	1.325IleIle: 1.325 ± 0.036	1.287IleLys: 1.287 ± 0.034	2.841IleLeu: 2.841 ± 0.054	0.632IleMet: 0.632 ± 0.025	1.221IleAsn: 1.221 ± 0.034	1.791IlePro: 1.791 ± 0.04	1.232IleGln: 1.232 ± 0.027	2.326IleArg: 2.326 ± 0.041	2.011IleSer: 2.011 ± 0.046	2.39IleThr: 2.39 ± 0.046	3.244IleVal: 3.244 ± 0.05	0.399IleTrp: 0.399 ± 0.019	0.863IleTyr: 0.863 ± 0.028	0.0IleXaa: 0.0 ± 0.0
Lys	3.646LysAla: 3.646 ± 0.079	0.123LysCys: 0.123 ± 0.01	1.465LysAsp: 1.465 ± 0.039	1.35LysGlu: 1.35 ± 0.036	0.759LysPhe: 0.759 ± 0.027	2.091LysGly: 2.091 ± 0.052	0.538LysHis: 0.538 ± 0.02	1.255LysIle: 1.255 ± 0.036	1.191LysLys: 1.191 ± 0.049	2.888LysLeu: 2.888 ± 0.061	0.696LysMet: 0.696 ± 0.024	0.773LysAsn: 0.773 ± 0.025	1.77LysPro: 1.77 ± 0.045	1.038LysGln: 1.038 ± 0.03	1.764LysArg: 1.764 ± 0.043	1.531LysSer: 1.531 ± 0.045	1.679LysThr: 1.679 ± 0.044	2.137LysVal: 2.137 ± 0.054	0.284LysTrp: 0.284 ± 0.016	0.554LysTyr: 0.554 ± 0.022	0.0LysXaa: 0.0 ± 0.0
Leu	15.68LeuAla: 15.68 ± 0.146	1.024LeuCys: 1.024 ± 0.028	5.474LeuAsp: 5.474 ± 0.076	5.059LeuGlu: 5.059 ± 0.07	3.145LeuPhe: 3.145 ± 0.053	8.873LeuGly: 8.873 ± 0.096	2.453LeuHis: 2.453 ± 0.053	3.873LeuIle: 3.873 ± 0.066	3.044LeuLys: 3.044 ± 0.055	11.352LeuLeu: 11.352 ± 0.131	2.449LeuMet: 2.449 ± 0.048	2.495LeuAsn: 2.495 ± 0.049	6.613LeuPro: 6.613 ± 0.082	5.138LeuGln: 5.138 ± 0.071	8.192LeuArg: 8.192 ± 0.094	5.812LeuSer: 5.812 ± 0.08	5.203LeuThr: 5.203 ± 0.07	8.008LeuVal: 8.008 ± 0.099	1.392LeuTrp: 1.392 ± 0.033	1.971LeuTyr: 1.971 ± 0.037	0.002LeuXaa: 0.002 ± 0.001
Met	3.211MetAla: 3.211 ± 0.05	0.148MetCys: 0.148 ± 0.01	1.112MetAsp: 1.112 ± 0.028	1.076MetGlu: 1.076 ± 0.026	0.657MetPhe: 0.657 ± 0.023	1.975MetGly: 1.975 ± 0.041	0.544MetHis: 0.544 ± 0.021	0.782MetIle: 0.782 ± 0.026	0.843MetLys: 0.843 ± 0.03	2.52MetLeu: 2.52 ± 0.048	0.498MetMet: 0.498 ± 0.022	0.717MetAsn: 0.717 ± 0.023	1.512MetPro: 1.512 ± 0.03	1.163MetGln: 1.163 ± 0.03	1.634MetArg: 1.634 ± 0.039	1.356MetSer: 1.356 ± 0.034	1.491MetThr: 1.491 ± 0.037	1.733MetVal: 1.733 ± 0.038	0.192MetTrp: 0.192 ± 0.012	0.336MetTyr: 0.336 ± 0.018	0.0MetXaa: 0.0 ± 0.0
Asn	3.226AsnAla: 3.226 ± 0.058	0.207AsnCys: 0.207 ± 0.012	1.197AsnAsp: 1.197 ± 0.031	0.994AsnGlu: 0.994 ± 0.028	0.827AsnPhe: 0.827 ± 0.025	1.961AsnGly: 1.961 ± 0.044	0.504AsnHis: 0.504 ± 0.021	1.029AsnIle: 1.029 ± 0.03	0.66AsnLys: 0.66 ± 0.025	2.362AsnLeu: 2.362 ± 0.042	0.483AsnMet: 0.483 ± 0.019	0.605AsnAsn: 0.605 ± 0.024	1.669AsnPro: 1.669 ± 0.036	0.844AsnGln: 0.844 ± 0.027	1.516AsnArg: 1.516 ± 0.039	1.017AsnSer: 1.017 ± 0.04	1.298AsnThr: 1.298 ± 0.041	1.847AsnVal: 1.847 ± 0.043	0.354AsnTrp: 0.354 ± 0.016	0.611AsnTyr: 0.611 ± 0.024	0.001AsnXaa: 0.001 ± 0.001
Pro	8.936ProAla: 8.936 ± 0.13	0.366ProCys: 0.366 ± 0.016	3.138ProAsp: 3.138 ± 0.056	3.273ProGlu: 3.273 ± 0.061	1.786ProPhe: 1.786 ± 0.037	5.308ProGly: 5.308 ± 0.071	1.282ProHis: 1.282 ± 0.032	1.769ProIle: 1.769 ± 0.035	1.197ProLys: 1.197 ± 0.032	5.709ProLeu: 5.709 ± 0.068	1.256ProMet: 1.256 ± 0.031	1.063ProAsn: 1.063 ± 0.031	3.13ProPro: 3.13 ± 0.076	2.414ProGln: 2.414 ± 0.048	3.25ProArg: 3.25 ± 0.055	2.873ProSer: 2.873 ± 0.047	2.614ProThr: 2.614 ± 0.047	4.565ProVal: 4.565 ± 0.062	0.837ProTrp: 0.837 ± 0.026	1.149ProTyr: 1.149 ± 0.033	0.001ProXaa: 0.001 ± 0.001
Gln	6.534GlnAla: 6.534 ± 0.093	0.29GlnCys: 0.29 ± 0.013	1.77GlnAsp: 1.77 ± 0.036	1.808GlnGlu: 1.808 ± 0.044	1.268GlnPhe: 1.268 ± 0.029	3.594GlnGly: 3.594 ± 0.061	1.02GlnHis: 1.02 ± 0.03	1.683GlnIle: 1.683 ± 0.037	1.04GlnLys: 1.04 ± 0.03	4.564GlnLeu: 4.564 ± 0.067	0.974GlnMet: 0.974 ± 0.028	0.891GlnAsn: 0.891 ± 0.032	2.753GlnPro: 2.753 ± 0.05	2.208GlnGln: 2.208 ± 0.054	3.81GlnArg: 3.81 ± 0.062	2.145GlnSer: 2.145 ± 0.042	1.936GlnThr: 1.936 ± 0.047	3.233GlnVal: 3.233 ± 0.046	0.748GlnTrp: 0.748 ± 0.028	0.775GlnTyr: 0.775 ± 0.027	0.0GlnXaa: 0.0 ± 0.0
Arg	8.769ArgAla: 8.769 ± 0.093	0.599ArgCys: 0.599 ± 0.022	3.539ArgAsp: 3.539 ± 0.06	3.866ArgGlu: 3.866 ± 0.058	2.709ArgPhe: 2.709 ± 0.045	4.933ArgGly: 4.933 ± 0.065	1.927ArgHis: 1.927 ± 0.039	3.312ArgIle: 3.312 ± 0.052	1.841ArgLys: 1.841 ± 0.041	8.173ArgLeu: 8.173 ± 0.092	1.99ArgMet: 1.99 ± 0.041	1.728ArgAsn: 1.728 ± 0.035	3.589ArgPro: 3.589 ± 0.053	3.269ArgGln: 3.269 ± 0.049	5.4ArgArg: 5.4 ± 0.068	3.705ArgSer: 3.705 ± 0.056	3.545ArgThr: 3.545 ± 0.052	5.261ArgVal: 5.261 ± 0.064	1.373ArgTrp: 1.373 ± 0.036	1.717ArgTyr: 1.717 ± 0.036	0.0ArgXaa: 0.0 ± 0.0
Ser	6.955SerAla: 6.955 ± 0.079	0.375SerCys: 0.375 ± 0.016	2.458SerAsp: 2.458 ± 0.051	2.163SerGlu: 2.163 ± 0.048	1.846SerPhe: 1.846 ± 0.036	5.254SerGly: 5.254 ± 0.077	1.224SerHis: 1.224 ± 0.029	2.193SerIle: 2.193 ± 0.039	1.27SerLys: 1.27 ± 0.036	5.523SerLeu: 5.523 ± 0.075	1.188SerMet: 1.188 ± 0.027	1.194SerAsn: 1.194 ± 0.034	3.035SerPro: 3.035 ± 0.055	1.934SerGln: 1.934 ± 0.042	3.406SerArg: 3.406 ± 0.053	2.908SerSer: 2.908 ± 0.055	2.889SerThr: 2.889 ± 0.051	3.921SerVal: 3.921 ± 0.06	0.724SerTrp: 0.724 ± 0.024	1.167SerTyr: 1.167 ± 0.03	0.0SerXaa: 0.0 ± 0.0
Thr	6.939ThrAla: 6.939 ± 0.09	0.369ThrCys: 0.369 ± 0.018	2.554ThrAsp: 2.554 ± 0.044	2.257ThrGlu: 2.257 ± 0.047	1.628ThrPhe: 1.628 ± 0.039	4.768ThrGly: 4.768 ± 0.067	1.176ThrHis: 1.176 ± 0.029	1.886ThrIle: 1.886 ± 0.042	1.097ThrLys: 1.097 ± 0.034	6.326ThrLeu: 6.326 ± 0.077	1.009ThrMet: 1.009 ± 0.03	1.072ThrAsn: 1.072 ± 0.036	3.832ThrPro: 3.832 ± 0.052	2.147ThrGln: 2.147 ± 0.037	3.308ThrArg: 3.308 ± 0.058	2.599ThrSer: 2.599 ± 0.051	2.747ThrThr: 2.747 ± 0.063	4.395ThrVal: 4.395 ± 0.065	0.651ThrTrp: 0.651 ± 0.02	1.001ThrTyr: 1.001 ± 0.024	0.001ThrXaa: 0.001 ± 0.001
Val	10.239ValAla: 10.239 ± 0.098	0.705ValCys: 0.705 ± 0.021	4.003ValAsp: 4.003 ± 0.048	4.046ValGlu: 4.046 ± 0.056	2.641ValPhe: 2.641 ± 0.05	5.767ValGly: 5.767 ± 0.079	1.637ValHis: 1.637 ± 0.036	3.11ValIle: 3.11 ± 0.048	2.183ValLys: 2.183 ± 0.051	8.513ValLeu: 8.513 ± 0.095	1.87ValMet: 1.87 ± 0.039	2.004ValAsn: 2.004 ± 0.038	4.51ValPro: 4.51 ± 0.065	3.324ValGln: 3.324 ± 0.057	5.524ValArg: 5.524 ± 0.077	3.846ValSer: 3.846 ± 0.048	4.125ValThr: 4.125 ± 0.058	6.57ValVal: 6.57 ± 0.085	0.997ValTrp: 0.997 ± 0.028	1.467ValTyr: 1.467 ± 0.033	0.0ValXaa: 0.0 ± 0.0
Trp	1.57TrpAla: 1.57 ± 0.041	0.16TrpCys: 0.16 ± 0.011	0.626TrpAsp: 0.626 ± 0.023	0.54TrpGlu: 0.54 ± 0.018	0.52TrpPhe: 0.52 ± 0.022	1.059TrpGly: 1.059 ± 0.031	0.38TrpHis: 0.38 ± 0.017	0.613TrpIle: 0.613 ± 0.024	0.437TrpLys: 0.437 ± 0.018	2.204TrpLeu: 2.204 ± 0.047	0.413TrpMet: 0.413 ± 0.017	0.456TrpAsn: 0.456 ± 0.018	0.785TrpPro: 0.785 ± 0.023	0.812TrpGln: 0.812 ± 0.028	1.301TrpArg: 1.301 ± 0.039	0.812TrpSer: 0.812 ± 0.026	0.766TrpThr: 0.766 ± 0.024	1.057TrpVal: 1.057 ± 0.031	0.285TrpTrp: 0.285 ± 0.015	0.261TrpTyr: 0.261 ± 0.014	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.604TyrAla: 2.604 ± 0.042	0.198TyrCys: 0.198 ± 0.011	1.182TyrAsp: 1.182 ± 0.03	1.076TyrGlu: 1.076 ± 0.032	0.838TyrPhe: 0.838 ± 0.024	1.974TyrGly: 1.974 ± 0.043	0.414TyrHis: 0.414 ± 0.019	0.687TyrIle: 0.687 ± 0.022	0.588TyrLys: 0.588 ± 0.023	2.294TyrLeu: 2.294 ± 0.045	0.372TyrMet: 0.372 ± 0.019	0.512TyrAsn: 0.512 ± 0.026	1.048TyrPro: 1.048 ± 0.031	0.892TyrGln: 0.892 ± 0.026	1.581TyrArg: 1.581 ± 0.034	1.097TyrSer: 1.097 ± 0.03	1.149TyrThr: 1.149 ± 0.032	1.548TyrVal: 1.548 ± 0.03	0.336TyrTrp: 0.336 ± 0.016	0.541TyrTyr: 0.541 ± 0.023	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.001XaaAsp: 0.001 ± 0.001	0.0XaaGlu: 0.0 ± 0.0	0.001XaaPhe: 0.001 ± 0.001	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.002XaaLeu: 0.002 ± 0.002	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.001XaaGln: 0.001 ± 0.001	0.002XaaArg: 0.002 ± 0.001	0.001XaaSer: 0.001 ± 0.001	0.0XaaThr: 0.0 ± 0.0	0.001XaaVal: 0.001 ± 0.001	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3935 proteins (1329603 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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