Panthera leo persica papillomavirus 1
Average proteome isoelectric point is 6.31
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|I6LEJ8|I6LEJ8_9PAPI Replication protein E1 OS=Panthera leo persica papillomavirus 1 OX=2772508 GN=E1 PE=3 SV=1
MM1 pKa = 7.57 IGVAPTIGDD10 pKa = 3.34 IVLTEE15 pKa = 3.91 QSPEE19 pKa = 4.21 TVDD22 pKa = 3.46 LRR24 pKa = 11.84 CYY26 pKa = 9.66 EE27 pKa = 3.96 QMPGEE32 pKa = 4.14 EE33 pKa = 4.71 EE34 pKa = 4.05 EE35 pKa = 4.75 VEE37 pKa = 4.35 SQSRR41 pKa = 11.84 DD42 pKa = 3.36 LYY44 pKa = 10.73 RR45 pKa = 11.84 VSASCGLCGSGVRR58 pKa = 11.84 FACLAAGEE66 pKa = 4.46 EE67 pKa = 4.37 ILRR70 pKa = 11.84 LHH72 pKa = 6.06 QLLFTLQFVCVPCVKK87 pKa = 9.83 QQKK90 pKa = 9.38 LNHH93 pKa = 6.38 GGG95 pKa = 3.28
Molecular weight: 10.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.501
IPC2_protein 4.813
IPC_protein 4.635
Toseland 4.52
ProMoST 4.724
Dawson 4.571
Bjellqvist 4.711
Wikipedia 4.406
Rodwell 4.495
Grimsley 4.444
Solomon 4.558
Lehninger 4.52
Nozaki 4.685
DTASelect 4.749
Thurlkill 4.52
EMBOSS 4.431
Sillero 4.762
Patrickios 2.028
IPC_peptide 4.571
IPC2_peptide 4.749
IPC2.peptide.svr19 4.706
Protein with the highest isoelectric point:
>tr|I6LEK0|I6LEK0_9PAPI E4 protein (Fragment) OS=Panthera leo persica papillomavirus 1 OX=2772508 GN=E4 PE=4 SV=1
MM1 pKa = 7.49 EE2 pKa = 4.93 NVSKK6 pKa = 10.78 ALEE9 pKa = 4.3 CVQEE13 pKa = 4.19 QLLHH17 pKa = 7.32 LYY19 pKa = 9.91 EE20 pKa = 5.39 KK21 pKa = 10.77 DD22 pKa = 3.45 SAEE25 pKa = 3.98 LRR27 pKa = 11.84 DD28 pKa = 5.84 QIMHH32 pKa = 6.38 WNLNRR37 pKa = 11.84 RR38 pKa = 11.84 EE39 pKa = 3.87 QALYY43 pKa = 10.12 YY44 pKa = 9.9 CARR47 pKa = 11.84 KK48 pKa = 9.81 QGITRR53 pKa = 11.84 IGMNPVPTLAAAQQKK68 pKa = 9.81 AKK70 pKa = 10.67 SAIEE74 pKa = 3.95 QEE76 pKa = 4.57 LLLQSLLDD84 pKa = 3.5 SKK86 pKa = 11.41 YY87 pKa = 11.05 ADD89 pKa = 4.1 EE90 pKa = 5.2 PWTLMDD96 pKa = 3.74 TSRR99 pKa = 11.84 EE100 pKa = 3.9 RR101 pKa = 11.84 LLVEE105 pKa = 4.33 PPYY108 pKa = 10.47 CFKK111 pKa = 11.05 KK112 pKa = 10.54 GGQQVEE118 pKa = 4.22 VRR120 pKa = 11.84 FDD122 pKa = 3.62 CDD124 pKa = 2.95 RR125 pKa = 11.84 EE126 pKa = 4.23 NVSRR130 pKa = 11.84 YY131 pKa = 8.34 VLWTDD136 pKa = 3.86 IYY138 pKa = 10.83 HH139 pKa = 5.69 QTEE142 pKa = 3.46 QDD144 pKa = 2.7 QWRR147 pKa = 11.84 KK148 pKa = 7.71 TKK150 pKa = 10.44 GQADD154 pKa = 3.62 NRR156 pKa = 11.84 GLFYY160 pKa = 10.73 KK161 pKa = 10.29 DD162 pKa = 2.98 EE163 pKa = 4.99 KK164 pKa = 10.33 GVKK167 pKa = 9.08 VYY169 pKa = 10.94 YY170 pKa = 10.71 VDD172 pKa = 6.07 FEE174 pKa = 4.98 DD175 pKa = 3.97 EE176 pKa = 4.06 AKK178 pKa = 10.79 KK179 pKa = 10.43 FGKK182 pKa = 8.2 TGHH185 pKa = 6.17 YY186 pKa = 10.38 DD187 pKa = 3.05 IVSKK191 pKa = 9.76 LTTPVLTSTTGSGLGDD207 pKa = 3.46 SSTAGSATASGCPKK221 pKa = 10.22 KK222 pKa = 9.55 QTPTKK227 pKa = 10.1 ARR229 pKa = 11.84 KK230 pKa = 9.16 RR231 pKa = 11.84 PLRR234 pKa = 11.84 LSSPKK239 pKa = 9.65 TSGSRR244 pKa = 11.84 FRR246 pKa = 11.84 RR247 pKa = 11.84 GGGGQRR253 pKa = 11.84 EE254 pKa = 4.34 LASSSSTPRR263 pKa = 11.84 PTPPSPGEE271 pKa = 3.74 VGKK274 pKa = 10.52 AVSTVPRR281 pKa = 11.84 RR282 pKa = 11.84 SGDD285 pKa = 3.13 RR286 pKa = 11.84 LGRR289 pKa = 11.84 LLLDD293 pKa = 3.81 ARR295 pKa = 11.84 DD296 pKa = 3.87 PPVLVLKK303 pKa = 10.69 GDD305 pKa = 4.11 PNSLKK310 pKa = 9.42 CTRR313 pKa = 11.84 YY314 pKa = 9.34 RR315 pKa = 11.84 LKK317 pKa = 10.82 GKK319 pKa = 10.1 YY320 pKa = 9.19 SHH322 pKa = 7.02 LFCRR326 pKa = 11.84 ASTTWQWTSSTGPDD340 pKa = 2.36 RR341 pKa = 11.84 WGRR344 pKa = 11.84 SRR346 pKa = 11.84 MLLTFTDD353 pKa = 3.83 TAQRR357 pKa = 11.84 DD358 pKa = 3.95 LFEE361 pKa = 4.91 KK362 pKa = 10.44 AVKK365 pKa = 10.11 LPKK368 pKa = 9.96 SVQFFRR374 pKa = 11.84 GSFEE378 pKa = 4.24 DD379 pKa = 3.67 LL380 pKa = 3.5
Molecular weight: 42.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.982
IPC2_protein 8.946
IPC_protein 8.858
Toseland 9.648
ProMoST 9.37
Dawson 9.897
Bjellqvist 9.604
Wikipedia 10.058
Rodwell 10.248
Grimsley 9.955
Solomon 9.926
Lehninger 9.882
Nozaki 9.765
DTASelect 9.56
Thurlkill 9.75
EMBOSS 10.072
Sillero 9.838
Patrickios 5.842
IPC_peptide 9.911
IPC2_peptide 8.258
IPC2.peptide.svr19 7.995
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
2341
95
606
334.4
37.52
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.109 ± 0.598
2.52 ± 0.787
5.809 ± 0.412
6.408 ± 0.363
4.742 ± 0.544
6.408 ± 0.558
1.623 ± 0.184
3.46 ± 0.545
5.51 ± 0.705
9.654 ± 0.568
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.41 ± 0.23
3.503 ± 0.909
6.664 ± 1.296
5.34 ± 0.267
6.408 ± 0.788
7.604 ± 0.884
5.895 ± 0.539
6.92 ± 0.569
1.367 ± 0.306
2.648 ± 0.274
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here