Lake Sarah-associated circular molecule 12

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 8.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A140AQP3|A0A140AQP3_9VIRU Uncharacterized protein OS=Lake Sarah-associated circular molecule 12 OX=1809112 PE=4 SV=1
MM1 pKa = 7.66PGVKK5 pKa = 10.13EE6 pKa = 3.82SDD8 pKa = 2.63KK9 pKa = 10.5WFIRR13 pKa = 11.84ITAPHH18 pKa = 6.22TLLRR22 pKa = 11.84PKK24 pKa = 10.43VEE26 pKa = 4.03EE27 pKa = 4.26LKK29 pKa = 11.04GQIDD33 pKa = 4.18IKK35 pKa = 10.93RR36 pKa = 11.84MAIGFHH42 pKa = 5.96IGNRR46 pKa = 11.84TGKK49 pKa = 8.51EE50 pKa = 3.97HH51 pKa = 6.3IHH53 pKa = 6.49IALEE57 pKa = 4.02LLGNLQQQSINKK69 pKa = 9.06RR70 pKa = 11.84MKK72 pKa = 10.43KK73 pKa = 9.58IFGVSGADD81 pKa = 3.38YY82 pKa = 10.8SSKK85 pKa = 10.09IWDD88 pKa = 3.93GSHH91 pKa = 6.76KK92 pKa = 10.23VLSYY96 pKa = 10.67LYY98 pKa = 9.23HH99 pKa = 6.91DD100 pKa = 4.13EE101 pKa = 4.5KK102 pKa = 11.48GSVEE106 pKa = 4.09YY107 pKa = 10.4FKK109 pKa = 10.9MEE111 pKa = 4.21LTPSEE116 pKa = 4.7LEE118 pKa = 4.29EE119 pKa = 3.93IMKK122 pKa = 9.9TKK124 pKa = 10.45DD125 pKa = 3.1VYY127 pKa = 11.4KK128 pKa = 10.61DD129 pKa = 3.12IVHH132 pKa = 6.29TAKK135 pKa = 10.44QKK137 pKa = 9.74AATRR141 pKa = 11.84IPEE144 pKa = 4.68RR145 pKa = 11.84ILEE148 pKa = 4.44EE149 pKa = 3.8IRR151 pKa = 11.84EE152 pKa = 4.33SGSTWTMRR160 pKa = 11.84QIIKK164 pKa = 10.47RR165 pKa = 11.84IFVGVNKK172 pKa = 10.35DD173 pKa = 2.62EE174 pKa = 4.38WYY176 pKa = 9.91PPGSQMEE183 pKa = 4.37RR184 pKa = 11.84YY185 pKa = 8.78VQEE188 pKa = 4.11ILVKK192 pKa = 10.37QNPEE196 pKa = 3.75DD197 pKa = 4.3SIPALTDD204 pKa = 3.1YY205 pKa = 11.14YY206 pKa = 10.92LARR209 pKa = 11.84FTYY212 pKa = 10.63GLL214 pKa = 3.75

Molecular weight: 24.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A140AQP3|A0A140AQP3_9VIRU Uncharacterized protein OS=Lake Sarah-associated circular molecule 12 OX=1809112 PE=4 SV=1
MM1 pKa = 7.66PPGRR5 pKa = 11.84KK6 pKa = 8.66YY7 pKa = 10.33RR8 pKa = 11.84KK9 pKa = 9.49KK10 pKa = 9.13GSKK13 pKa = 9.1PKK15 pKa = 9.98RR16 pKa = 11.84VLMPYY21 pKa = 9.45TPARR25 pKa = 11.84KK26 pKa = 8.48YY27 pKa = 10.18KK28 pKa = 10.24KK29 pKa = 9.86RR30 pKa = 11.84GGRR33 pKa = 11.84STAGRR38 pKa = 11.84LITTMAGLLTNSTWTQKK55 pKa = 10.1ARR57 pKa = 11.84KK58 pKa = 9.22LGFQARR64 pKa = 11.84AMRR67 pKa = 11.84AVGAPNIYY75 pKa = 9.57HH76 pKa = 7.19ANFALPVVVPPGLQDD91 pKa = 4.88FISFPTLQRR100 pKa = 11.84TQLQAILSSVPQSSGLNRR118 pKa = 11.84ALIQSAQTEE127 pKa = 4.44LTFTNVTNAPVQVEE141 pKa = 4.48MYY143 pKa = 10.47DD144 pKa = 2.94IMFRR148 pKa = 11.84RR149 pKa = 11.84DD150 pKa = 3.35LSTDD154 pKa = 3.04ALVVNNGNNFTFATIEE170 pKa = 3.91EE171 pKa = 4.58MIKK174 pKa = 10.46SGCQAANGIAPGGTDD189 pKa = 3.03VSSYY193 pKa = 10.43IGASAYY199 pKa = 10.32DD200 pKa = 3.47SQIFKK205 pKa = 10.41DD206 pKa = 3.4YY207 pKa = 10.78CRR209 pKa = 11.84VVKK212 pKa = 10.27RR213 pKa = 11.84QHH215 pKa = 5.66VLLASGATHH224 pKa = 7.0RR225 pKa = 11.84HH226 pKa = 4.45QSNIGINKK234 pKa = 9.73IIDD237 pKa = 3.59EE238 pKa = 4.38SVGANEE244 pKa = 4.26DD245 pKa = 3.8LIYY248 pKa = 11.21VKK250 pKa = 10.61GYY252 pKa = 9.25TYY254 pKa = 10.4ATLILTRR261 pKa = 11.84GVGAFDD267 pKa = 3.59ATASVPNSTTNGAFLNFVTSVRR289 pKa = 11.84IKK291 pKa = 8.11YY292 pKa = 7.4TWVADD297 pKa = 3.91TTNSVYY303 pKa = 10.83YY304 pKa = 10.6HH305 pKa = 6.68NLPLHH310 pKa = 5.87TGVPNVRR317 pKa = 11.84NTGSGAYY324 pKa = 9.56EE325 pKa = 4.2SEE327 pKa = 4.55TPP329 pKa = 3.8

Molecular weight: 35.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

543

214

329

271.5

30.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.366 ± 1.919

0.368 ± 0.224

3.867 ± 0.489

4.972 ± 2.371

3.315 ± 0.31

7.366 ± 0.5

2.394 ± 0.532

7.182 ± 1.597

6.814 ± 1.821

7.182 ± 0.179

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.578 ± 0.421

4.788 ± 1.488

4.788 ± 0.354

4.236 ± 0.018

5.893 ± 0.173

6.814 ± 0.449

7.735 ± 1.575

6.63 ± 0.904

1.105 ± 0.464

4.604 ± 0.042
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski