Wenzhou narna-like virus 5

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KIS9|A0A1L3KIS9_9VIRU Capsid protein OS=Wenzhou narna-like virus 5 OX=1923580 PE=3 SV=1
MM1 pKa = 7.52SPLEE5 pKa = 4.26EE6 pKa = 4.47KK7 pKa = 10.68SSQKK11 pKa = 10.24RR12 pKa = 11.84IRR14 pKa = 11.84IKK16 pKa = 7.7THH18 pKa = 5.89EE19 pKa = 4.26FTSCGSTRR27 pKa = 11.84SFDD30 pKa = 3.4SGKK33 pKa = 10.19SLRR36 pKa = 11.84AFGSKK41 pKa = 9.79GAASMSRR48 pKa = 11.84AKK50 pKa = 10.76CRR52 pKa = 11.84DD53 pKa = 3.31SEE55 pKa = 4.14KK56 pKa = 11.09SKK58 pKa = 10.78ISVHH62 pKa = 4.16TTILSVLNKK71 pKa = 9.69VQTNNTEE78 pKa = 3.93VNVILEE84 pKa = 4.16SLLWPFVARR93 pKa = 11.84RR94 pKa = 11.84SQRR97 pKa = 11.84SEE99 pKa = 3.57MFSPKK104 pKa = 10.19EE105 pKa = 3.7FDD107 pKa = 3.5RR108 pKa = 11.84MVSSFKK114 pKa = 10.16KK115 pKa = 9.0TGEE118 pKa = 4.2TILKK122 pKa = 9.71YY123 pKa = 10.8VSADD127 pKa = 3.0NKK129 pKa = 8.15EE130 pKa = 4.13QKK132 pKa = 9.22FCKK135 pKa = 9.88YY136 pKa = 8.97WLDD139 pKa = 3.64YY140 pKa = 10.82YY141 pKa = 10.55MCLVFQDD148 pKa = 3.59HH149 pKa = 5.79QRR151 pKa = 11.84PEE153 pKa = 3.93KK154 pKa = 10.65PEE156 pKa = 3.89WLNSNLFSGWLKK168 pKa = 10.76RR169 pKa = 11.84FVCRR173 pKa = 11.84AIAKK177 pKa = 9.97DD178 pKa = 3.81DD179 pKa = 3.4VSFIYY184 pKa = 10.5SLQKK188 pKa = 9.97GSKK191 pKa = 9.4RR192 pKa = 11.84LWPRR196 pKa = 11.84LSKK199 pKa = 10.52QSEE202 pKa = 3.71YY203 pKa = 11.16DD204 pKa = 3.05AYY206 pKa = 10.88LKK208 pKa = 10.56HH209 pKa = 6.79ASRR212 pKa = 11.84LSSFHH217 pKa = 6.72GFLPDD222 pKa = 5.12DD223 pKa = 4.37LDD225 pKa = 4.11EE226 pKa = 5.16EE227 pKa = 4.54ISRR230 pKa = 11.84TSQEE234 pKa = 3.1IFLPINSGKK243 pKa = 9.19MEE245 pKa = 4.33ASKK248 pKa = 11.03YY249 pKa = 10.0CPTGSGCLQATKK261 pKa = 11.05NEE263 pKa = 3.97FGALSLLPTHH273 pKa = 6.84FEE275 pKa = 4.13VSDD278 pKa = 4.98DD279 pKa = 3.7IPNASSLGKK288 pKa = 10.18LRR290 pKa = 11.84ALNHH294 pKa = 5.88SFIKK298 pKa = 10.39YY299 pKa = 9.12RR300 pKa = 11.84QEE302 pKa = 4.82SYY304 pKa = 11.51DD305 pKa = 3.48QMLSKK310 pKa = 10.42VHH312 pKa = 6.53KK313 pKa = 9.88SLVDD317 pKa = 3.31AEE319 pKa = 5.1SIFDD323 pKa = 4.04LKK325 pKa = 10.74CVAVAEE331 pKa = 4.21PSKK334 pKa = 10.92FRR336 pKa = 11.84MITKK340 pKa = 10.3GDD342 pKa = 3.54GYY344 pKa = 10.75LYY346 pKa = 10.12TALQPLQGQMLDD358 pKa = 3.12CWKK361 pKa = 10.1KK362 pKa = 10.16SKK364 pKa = 10.74YY365 pKa = 9.31STMLEE370 pKa = 3.59QDD372 pKa = 3.27FEE374 pKa = 4.52KK375 pKa = 10.6RR376 pKa = 11.84IQTIDD381 pKa = 3.28KK382 pKa = 9.36NCRR385 pKa = 11.84ILEE388 pKa = 4.22FWCSVDD394 pKa = 4.11YY395 pKa = 10.8EE396 pKa = 4.32AATDD400 pKa = 4.92LIKK403 pKa = 10.9KK404 pKa = 8.89QATLTAIKK412 pKa = 10.14EE413 pKa = 3.93IDD415 pKa = 3.34HH416 pKa = 6.79KK417 pKa = 11.11YY418 pKa = 10.96SEE420 pKa = 4.45LAFLALSASGQCQYY434 pKa = 10.76PQLKK438 pKa = 9.55GFPKK442 pKa = 10.09IEE444 pKa = 5.8DD445 pKa = 3.39ITCIDD450 pKa = 4.06SQLMGHH456 pKa = 6.95PLSFPLLCTINLAVFRR472 pKa = 11.84CALKK476 pKa = 10.42RR477 pKa = 11.84WVNDD481 pKa = 3.32SPNSFEE487 pKa = 3.88KK488 pKa = 10.36RR489 pKa = 11.84CRR491 pKa = 11.84KK492 pKa = 8.9EE493 pKa = 3.68RR494 pKa = 11.84AEE496 pKa = 3.94LMYY499 pKa = 11.35NNVLVNGDD507 pKa = 3.99DD508 pKa = 3.69MLFKK512 pKa = 10.75CEE514 pKa = 3.63RR515 pKa = 11.84SFYY518 pKa = 10.52EE519 pKa = 3.97IFVKK523 pKa = 9.89TALDD527 pKa = 3.89CGLKK531 pKa = 9.55MSQGKK536 pKa = 9.68QYY538 pKa = 11.16LSVDD542 pKa = 2.85HH543 pKa = 6.92CMINSLVFKK552 pKa = 10.69RR553 pKa = 11.84VGNLMRR559 pKa = 11.84NQGYY563 pKa = 10.53LNLKK567 pKa = 9.35VISNEE572 pKa = 3.89SLKK575 pKa = 11.21DD576 pKa = 3.79GVSLCLPTQISKK588 pKa = 9.16TINEE592 pKa = 4.31MCSLVPWACCIIPTVFKK609 pKa = 10.51RR610 pKa = 11.84WSSDD614 pKa = 3.11LKK616 pKa = 10.98FLNFSPNWFLPCHH629 pKa = 6.46LGGFGVEE636 pKa = 3.75RR637 pKa = 11.84RR638 pKa = 11.84FGSVFKK644 pKa = 9.85LTRR647 pKa = 11.84QQRR650 pKa = 11.84KK651 pKa = 6.94VCTMFVKK658 pKa = 10.62NPEE661 pKa = 3.73LSLYY665 pKa = 6.99FTKK668 pKa = 10.7GFSLDD673 pKa = 3.06HH674 pKa = 6.44FYY676 pKa = 11.29KK677 pKa = 10.72FKK679 pKa = 10.96SQISGALSHH688 pKa = 6.95PRR690 pKa = 11.84FKK692 pKa = 10.73QSYY695 pKa = 9.7CPLNDD700 pKa = 4.02NEE702 pKa = 5.01CSDD705 pKa = 4.81DD706 pKa = 3.43PWLEE710 pKa = 4.26RR711 pKa = 11.84ISLIHH716 pKa = 6.54RR717 pKa = 11.84FSSFALRR724 pKa = 11.84KK725 pKa = 10.21NEE727 pKa = 3.91VAKK730 pKa = 10.8LRR732 pKa = 11.84EE733 pKa = 4.16FGKK736 pKa = 9.87EE737 pKa = 3.56LYY739 pKa = 10.14RR740 pKa = 11.84FRR742 pKa = 11.84TMPRR746 pKa = 11.84EE747 pKa = 3.95KK748 pKa = 10.72VDD750 pKa = 3.52TLVHH754 pKa = 6.22LKK756 pKa = 10.08FVSIKK761 pKa = 10.91GPICPPLPPLKK772 pKa = 10.55LRR774 pKa = 11.84TLNSHH779 pKa = 6.45IEE781 pKa = 4.11SSEE784 pKa = 4.0FPP786 pKa = 3.72

Molecular weight: 90.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KIS9|A0A1L3KIS9_9VIRU Capsid protein OS=Wenzhou narna-like virus 5 OX=1923580 PE=3 SV=1
MM1 pKa = 7.35YY2 pKa = 10.47SPSQSLKK9 pKa = 10.86HH10 pKa = 6.48PILMIKK16 pKa = 9.8SPKK19 pKa = 7.81TANVPMKK26 pKa = 9.41EE27 pKa = 3.87AKK29 pKa = 9.2PRR31 pKa = 11.84RR32 pKa = 11.84RR33 pKa = 11.84RR34 pKa = 11.84SRR36 pKa = 11.84PKK38 pKa = 10.01QPQQKK43 pKa = 9.5LQTEE47 pKa = 4.48INEE50 pKa = 4.2MAPVSYY56 pKa = 10.95ASTRR60 pKa = 11.84SFRR63 pKa = 11.84TDD65 pKa = 2.59VLQSHH70 pKa = 6.91KK71 pKa = 10.35NVKK74 pKa = 9.92SRR76 pKa = 11.84EE77 pKa = 4.09QISSITGSNSFLVGGTFHH95 pKa = 7.43INPGLPEE102 pKa = 4.41CFPYY106 pKa = 10.93LSDD109 pKa = 3.41IAKK112 pKa = 10.19KK113 pKa = 8.75FQQYY117 pKa = 9.22RR118 pKa = 11.84FKK120 pKa = 11.1SLTFEE125 pKa = 4.65FVTSTATTTTGNVILSPEE143 pKa = 4.26YY144 pKa = 10.31NPKK147 pKa = 9.89EE148 pKa = 3.96LPPATEE154 pKa = 3.84QQATNTYY161 pKa = 8.96GAVEE165 pKa = 4.3SVCWRR170 pKa = 11.84NSKK173 pKa = 10.31VSFSPSGMFPLGPRR187 pKa = 11.84KK188 pKa = 9.23MIRR191 pKa = 11.84SCLVPSDD198 pKa = 3.88LQAYY202 pKa = 8.53DD203 pKa = 3.29AGQLFVCVTGQTGNPLIGKK222 pKa = 8.63LWVSYY227 pKa = 10.92DD228 pKa = 3.0VDD230 pKa = 4.86LYY232 pKa = 10.73IPQNSLVQDD241 pKa = 3.82YY242 pKa = 9.99SPSSAAMFTIVSTALTSGVATLLTLTTVYY271 pKa = 11.12DD272 pKa = 3.77PFGFTNNGNTLSLLKK287 pKa = 10.4GVYY290 pKa = 10.3RR291 pKa = 11.84MDD293 pKa = 3.45ARR295 pKa = 11.84FTVDD299 pKa = 3.1GASVTNCLTQFSVGGVLSTTHH320 pKa = 6.57TYY322 pKa = 10.33VAGSPTLWTYY332 pKa = 10.95FGTGLQMLIPSTEE345 pKa = 3.88VQNFGISVASTFTGAANAGNGVLIINLVV373 pKa = 3.2

Molecular weight: 40.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1159

373

786

579.5

65.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.832 ± 0.594

2.847 ± 0.839

4.142 ± 0.814

4.918 ± 1.097

5.953 ± 0.479

5.091 ± 1.047

1.812 ± 0.412

4.659 ± 0.206

7.593 ± 1.691

10.009 ± 0.796

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.416 ± 0.002

4.314 ± 0.434

5.091 ± 0.898

4.228 ± 0.482

5.004 ± 0.697

10.699 ± 0.014

6.126 ± 2.412

5.695 ± 1.159

1.294 ± 0.273

3.279 ± 0.264
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski