Alteromonadaceae bacterium 2052S.S.stab0a.01

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Alteromonadaceae; unclassified Alteromonadaceae

Average proteome isoelectric point is 5.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4578 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A495D8X9|A0A495D8X9_9ALTE Uncharacterized protein OS=Alteromonadaceae bacterium 2052S.S.stab0a.01 OX=1304900 GN=K256_1559 PE=4 SV=1
MM1 pKa = 7.31IQQNYY6 pKa = 8.48LFRR9 pKa = 11.84RR10 pKa = 11.84AAPVSALLLGSLFSLASTQALALQCSYY37 pKa = 10.87EE38 pKa = 3.99VSNDD42 pKa = 2.88WGSGFTANITINNDD56 pKa = 2.48GTTTVSDD63 pKa = 4.7WEE65 pKa = 4.55VQWQQNGGSTVTGLWNAAYY84 pKa = 8.73TGSNPYY90 pKa = 8.92TATDD94 pKa = 3.69AGWNANIAPGSSASFGFQGNGEE116 pKa = 4.52DD117 pKa = 3.49STGTILSCTAGGSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSGGNSTTLVIQEE208 pKa = 4.12DD209 pKa = 3.93TGLCTVDD216 pKa = 5.16GSIDD220 pKa = 3.72SNNAGFTGSGFANTDD235 pKa = 3.02NVIGSRR241 pKa = 11.84VEE243 pKa = 3.79YY244 pKa = 10.43SIYY247 pKa = 10.64AAAAGNVLLDD257 pKa = 3.43ARR259 pKa = 11.84YY260 pKa = 7.82ATTSSRR266 pKa = 11.84AGDD269 pKa = 3.59VEE271 pKa = 4.37INGVNVGAFDD281 pKa = 4.55FVSTGDD287 pKa = 3.22WTSWTTEE294 pKa = 4.2SINVPLQAGLNSVSLIATTDD314 pKa = 3.13SGLPNIDD321 pKa = 3.63SLTAIGNDD329 pKa = 4.31LSAGDD334 pKa = 4.63CGSGSSSSSSSSSSSGSSSSGGVFNTLVIEE364 pKa = 4.15EE365 pKa = 4.72EE366 pKa = 3.87EE367 pKa = 3.98AGYY370 pKa = 10.97CNTLSVPVEE379 pKa = 4.04NEE381 pKa = 3.67HH382 pKa = 6.59TGFTGSGYY390 pKa = 11.1ANSEE394 pKa = 4.09NATGTGLEE402 pKa = 4.23WQVTTGEE409 pKa = 4.57AGTATIDD416 pKa = 3.14VRR418 pKa = 11.84FANGSDD424 pKa = 3.32ADD426 pKa = 4.22RR427 pKa = 11.84PGTLYY432 pKa = 10.57INGGADD438 pKa = 3.24GAYY441 pKa = 9.17TISLPATGAWTTWQTSSISVPLPAGDD467 pKa = 4.2SSLEE471 pKa = 3.97LLADD475 pKa = 3.97TANGLANIDD484 pKa = 4.06SLTITALDD492 pKa = 4.08VSPLDD497 pKa = 3.98CAEE500 pKa = 4.45VPPEE504 pKa = 4.24VPDD507 pKa = 3.64EE508 pKa = 4.16LLAFPGAQGFGRR520 pKa = 11.84LAVGGRR526 pKa = 11.84YY527 pKa = 10.06GEE529 pKa = 4.46VVHH532 pKa = 5.63VTNLNDD538 pKa = 3.5SGTGSLRR545 pKa = 11.84DD546 pKa = 4.83AISQPNRR553 pKa = 11.84IVVFDD558 pKa = 3.53VGGVINLSSRR568 pKa = 11.84LVFKK572 pKa = 11.04NNQTIAGQTAPGGGITLYY590 pKa = 11.34GNGTSFSAASNTIVRR605 pKa = 11.84YY606 pKa = 9.42VRR608 pKa = 11.84FRR610 pKa = 11.84MGINGDD616 pKa = 3.75SGKK619 pKa = 9.05DD620 pKa = 3.49TITMANGHH628 pKa = 5.61DD629 pKa = 4.57VIWDD633 pKa = 3.6HH634 pKa = 7.4CSLSWGRR641 pKa = 11.84DD642 pKa = 2.94GTLDD646 pKa = 3.73LNQEE650 pKa = 4.39SGSTLYY656 pKa = 11.22NLTLQDD662 pKa = 3.93SIVAQGLQTHH672 pKa = 5.35STGGLVNTTGTSIIRR687 pKa = 11.84SLYY690 pKa = 9.43IDD692 pKa = 3.54NNSRR696 pKa = 11.84NPKK699 pKa = 10.16ARR701 pKa = 11.84GTLQFVNNVLYY712 pKa = 10.7NWVTSGYY719 pKa = 10.21ILGDD723 pKa = 3.17TSGRR727 pKa = 11.84SDD729 pKa = 2.98GAMIGNFLIAGPEE742 pKa = 4.32TNGGTLSSPTPEE754 pKa = 3.88YY755 pKa = 10.9NIYY758 pKa = 10.81AVDD761 pKa = 2.96NWYY764 pKa = 10.57DD765 pKa = 3.6NNKK768 pKa = 10.68DD769 pKa = 3.05GTLNARR775 pKa = 11.84VLGEE779 pKa = 4.18GDD781 pKa = 3.49FGSATWWTTPSVEE794 pKa = 4.36YY795 pKa = 8.75PTVPALSADD804 pKa = 3.27AALQYY809 pKa = 10.67VINNAGTSQWRR820 pKa = 11.84DD821 pKa = 3.17EE822 pKa = 4.2VDD824 pKa = 3.06QYY826 pKa = 12.13LMDD829 pKa = 5.0EE830 pKa = 4.2LTSWGRR836 pKa = 11.84QGATISNEE844 pKa = 3.82SSLGIPNNVGVIPGGTAPTDD864 pKa = 3.41TDD866 pKa = 3.86RR867 pKa = 11.84DD868 pKa = 3.77GMPDD872 pKa = 2.85SWEE875 pKa = 4.0TQMGFNPNNAADD887 pKa = 4.03AFADD891 pKa = 3.62ADD893 pKa = 3.73GDD895 pKa = 3.68GWVNLEE901 pKa = 4.18EE902 pKa = 4.66YY903 pKa = 10.42INSLVPP909 pKa = 3.73

Molecular weight: 93.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A495DIU6|A0A495DIU6_9ALTE Cell migration-inducing and hyaluronan-binding protein OS=Alteromonadaceae bacterium 2052S.S.stab0a.01 OX=1304900 GN=K256_4544 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLKK11 pKa = 10.2RR12 pKa = 11.84KK13 pKa = 7.57RR14 pKa = 11.84THH16 pKa = 5.9GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.2GRR39 pKa = 11.84KK40 pKa = 8.31QLAAA44 pKa = 4.14

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4578

0

4578

1627577

29

5920

355.5

39.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.466 ± 0.042

1.069 ± 0.014

5.919 ± 0.041

6.085 ± 0.036

3.95 ± 0.023

7.537 ± 0.04

2.186 ± 0.021

5.206 ± 0.026

3.792 ± 0.038

10.281 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.261 ± 0.017

3.908 ± 0.025

4.425 ± 0.031

4.258 ± 0.021

5.492 ± 0.033

7.047 ± 0.065

5.531 ± 0.036

7.091 ± 0.031

1.43 ± 0.015

3.065 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski