Serratia phage PCH45

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Iapetusvirus; unclassified Iapetusvirus

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 225 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5P8D3A2|A0A5P8D3A2_9CAUD Uncharacterized protein OS=Serratia phage PCH45 OX=2608368 PE=4 SV=1
MM1 pKa = 7.83SSITNNVVNTVTLTDD16 pKa = 3.63EE17 pKa = 4.52KK18 pKa = 11.16SFSAEE23 pKa = 3.84FIFSNKK29 pKa = 6.73EE30 pKa = 3.65TALHH34 pKa = 5.99GVHH37 pKa = 6.18TALLTILAGAAVEE50 pKa = 4.4QEE52 pKa = 4.39FNRR55 pKa = 11.84SNIFSCKK62 pKa = 9.76YY63 pKa = 10.07NDD65 pKa = 3.47VFTGNVTIGTRR76 pKa = 11.84GDD78 pKa = 3.7RR79 pKa = 11.84IIISDD84 pKa = 3.9GNSEE88 pKa = 4.19FDD90 pKa = 3.22ISCYY94 pKa = 9.02EE95 pKa = 3.75AQTYY99 pKa = 9.91ILEE102 pKa = 4.43PFGLFMPDD110 pKa = 3.67FVPMINEE117 pKa = 4.0HH118 pKa = 6.74LFGCEE123 pKa = 5.94GITCWMIEE131 pKa = 4.11EE132 pKa = 4.38LGGYY136 pKa = 10.14YY137 pKa = 10.17SGQDD141 pKa = 3.03SDD143 pKa = 4.84LWILTSQGMLIATEE157 pKa = 4.16EE158 pKa = 4.01LRR160 pKa = 11.84KK161 pKa = 10.08EE162 pKa = 4.2DD163 pKa = 3.48MSTYY167 pKa = 9.95LSPVAVYY174 pKa = 10.5DD175 pKa = 5.45LNHH178 pKa = 5.72QWTEE182 pKa = 4.1EE183 pKa = 3.91EE184 pKa = 4.61VKK186 pKa = 10.86NSEE189 pKa = 4.08QIKK192 pKa = 10.52AIIAASYY199 pKa = 10.18

Molecular weight: 22.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5P8D304|A0A5P8D304_9CAUD DNA-directed RNA polymerase OS=Serratia phage PCH45 OX=2608368 PE=3 SV=1
MM1 pKa = 6.85NLKK4 pKa = 9.64WFLLPVLLVMSFGILASTSTSTVKK28 pKa = 10.56FNEE31 pKa = 4.22KK32 pKa = 9.17GWNKK36 pKa = 9.36HH37 pKa = 4.93RR38 pKa = 11.84NAITSAAYY46 pKa = 7.72RR47 pKa = 11.84TNQPVDD53 pKa = 3.23ALAAFASIEE62 pKa = 4.24TTMGLSKK69 pKa = 10.55DD70 pKa = 3.54RR71 pKa = 11.84KK72 pKa = 10.27GLFQFVDD79 pKa = 3.82RR80 pKa = 11.84TWRR83 pKa = 11.84DD84 pKa = 3.26QVKK87 pKa = 10.68RR88 pKa = 11.84NGKK91 pKa = 9.87KK92 pKa = 8.78YY93 pKa = 10.23GIRR96 pKa = 11.84IGTPQTNARR105 pKa = 11.84ANALMAAEE113 pKa = 3.98YY114 pKa = 9.38MRR116 pKa = 11.84EE117 pKa = 3.62NRR119 pKa = 11.84EE120 pKa = 3.68YY121 pKa = 11.2LEE123 pKa = 4.51RR124 pKa = 11.84KK125 pKa = 9.48LGRR128 pKa = 11.84TVSLSEE134 pKa = 3.88IYY136 pKa = 8.7MAHH139 pKa = 6.19MLGTYY144 pKa = 9.55GAEE147 pKa = 4.18KK148 pKa = 9.7MLKK151 pKa = 10.26ASNGKK156 pKa = 8.74LAKK159 pKa = 10.58NIVRR163 pKa = 11.84PGGNKK168 pKa = 10.02AFFFTKK174 pKa = 10.17GKK176 pKa = 10.42ARR178 pKa = 11.84TVGQFKK184 pKa = 10.53QYY186 pKa = 9.61MEE188 pKa = 4.94AYY190 pKa = 8.99VEE192 pKa = 4.42RR193 pKa = 11.84HH194 pKa = 5.03ASTYY198 pKa = 10.8RR199 pKa = 11.84LTAVAYY205 pKa = 8.01MDD207 pKa = 3.5TRR209 pKa = 11.84FEE211 pKa = 4.42STTQLAMRR219 pKa = 5.2

Molecular weight: 24.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

225

0

225

66817

42

2274

297.0

33.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.116 ± 0.156

0.823 ± 0.057

6.705 ± 0.13

6.615 ± 0.167

4.156 ± 0.103

6.896 ± 0.245

1.675 ± 0.077

6.325 ± 0.116

5.934 ± 0.15

8.354 ± 0.127

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.785 ± 0.07

5.159 ± 0.109

4.325 ± 0.096

3.45 ± 0.095

5.564 ± 0.183

6.218 ± 0.114

5.798 ± 0.13

6.718 ± 0.112

1.405 ± 0.056

3.977 ± 0.112
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski