candidate division MSBL1 archaeon SCGC-AAA382C18

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Euryarchaeota incertae sedis; candidate division MSBL1

Average proteome isoelectric point is 6.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 551 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A133VKL2|A0A133VKL2_9EURY Citramalate synthase (Fragment) OS=candidate division MSBL1 archaeon SCGC-AAA382C18 OX=1698281 GN=AKJ52_01195 PE=3 SV=1
MM1 pKa = 7.49KK2 pKa = 10.18KK3 pKa = 9.61IKK5 pKa = 10.24EE6 pKa = 3.99YY7 pKa = 11.21VIFLLCAMFLSGLVGFFNIDD27 pKa = 2.86AVEE30 pKa = 4.31VVNEE34 pKa = 3.9TAIPPFYY41 pKa = 10.54NRR43 pKa = 11.84PEE45 pKa = 5.07AGDD48 pKa = 3.55SGFLAEE54 pKa = 5.9ANTEE58 pKa = 4.4DD59 pKa = 3.32ITPFDD64 pKa = 3.62EE65 pKa = 4.36MSNVVAPHH73 pKa = 6.59FDD75 pKa = 3.81DD76 pKa = 5.82VITGQSDD83 pKa = 4.02TPTGNSYY90 pKa = 9.71MLFEE94 pKa = 4.21EE95 pKa = 5.52HH96 pKa = 6.78GGDD99 pKa = 3.01WADD102 pKa = 4.92AEE104 pKa = 4.4KK105 pKa = 10.96LPNNDD110 pKa = 4.72EE111 pKa = 5.24DD112 pKa = 6.54DD113 pKa = 4.34LLCWALTASNVLEE126 pKa = 3.86WSGWGFVSDD135 pKa = 5.11FDD137 pKa = 5.4SSDD140 pKa = 3.42DD141 pKa = 3.39MGDD144 pKa = 3.43YY145 pKa = 10.74FEE147 pKa = 5.72SHH149 pKa = 5.33VTDD152 pKa = 3.21SGSWVIYY159 pKa = 9.28GWEE162 pKa = 3.4WWFTGNLPYY171 pKa = 10.94YY172 pKa = 10.52GMGWSYY178 pKa = 11.47EE179 pKa = 4.07DD180 pKa = 4.18EE181 pKa = 4.46NGGDD185 pKa = 3.73FWSDD189 pKa = 2.89EE190 pKa = 4.22YY191 pKa = 11.53DD192 pKa = 3.61YY193 pKa = 11.83SEE195 pKa = 4.29YY196 pKa = 10.61THH198 pKa = 6.63MVDD201 pKa = 4.79NSLKK205 pKa = 9.96TMEE208 pKa = 5.87AIDD211 pKa = 4.26NYY213 pKa = 10.91LHH215 pKa = 6.41NGWGAGLSLGDD226 pKa = 3.95HH227 pKa = 7.57AITVWGFNYY236 pKa = 10.27DD237 pKa = 3.51PSVDD241 pKa = 3.45KK242 pKa = 8.73TTNPHH247 pKa = 7.05DD248 pKa = 4.23YY249 pKa = 11.2YY250 pKa = 11.26LGIWVTDD257 pKa = 3.26SDD259 pKa = 4.77DD260 pKa = 4.55NKK262 pKa = 10.8SQSNPPDD269 pKa = 3.28MLHH272 pKa = 5.48YY273 pKa = 10.94CEE275 pKa = 5.51VEE277 pKa = 3.74WDD279 pKa = 3.61DD280 pKa = 4.95SYY282 pKa = 11.69PGYY285 pKa = 10.93YY286 pKa = 10.44LIPSYY291 pKa = 10.83GRR293 pKa = 11.84SIAMVYY299 pKa = 10.52ALEE302 pKa = 4.46PFPDD306 pKa = 3.67EE307 pKa = 4.2TRR309 pKa = 11.84PTSDD313 pKa = 2.88AGGSYY318 pKa = 9.19TVVEE322 pKa = 4.48GSPVTFDD329 pKa = 4.2ASGSLDD335 pKa = 4.36DD336 pKa = 6.05DD337 pKa = 4.11ALLYY341 pKa = 10.58RR342 pKa = 11.84WDD344 pKa = 4.19LNNDD348 pKa = 3.98RR349 pKa = 11.84VWDD352 pKa = 4.19TSWVSSPTASYY363 pKa = 8.48TWNDD367 pKa = 3.31DD368 pKa = 3.09NYY370 pKa = 11.13GEE372 pKa = 4.36VKK374 pKa = 10.78VEE376 pKa = 4.02VFDD379 pKa = 4.27GRR381 pKa = 11.84LRR383 pKa = 11.84DD384 pKa = 3.31VDD386 pKa = 3.67TATFNIINADD396 pKa = 3.59PTVTATVDD404 pKa = 3.02SDD406 pKa = 4.05TVDD409 pKa = 3.09EE410 pKa = 4.7NGAVTVSGTITDD422 pKa = 3.1SGTRR426 pKa = 11.84DD427 pKa = 3.44NFTVVIDD434 pKa = 3.62WGDD437 pKa = 4.16GISSSYY443 pKa = 10.76SYY445 pKa = 10.36PAGSTDD451 pKa = 3.16YY452 pKa = 11.4SEE454 pKa = 4.0THH456 pKa = 6.31QYY458 pKa = 11.41LDD460 pKa = 4.5DD461 pKa = 4.81NPTEE465 pKa = 4.33TPSDD469 pKa = 3.34NCTINVTVTDD479 pKa = 3.94DD480 pKa = 3.86DD481 pKa = 4.97YY482 pKa = 12.25GVGTANTKK490 pKa = 8.57VTVNNTKK497 pKa = 10.42PMVEE501 pKa = 4.16APFISLQQNSEE512 pKa = 4.3FILPVVHH519 pKa = 5.85EE520 pKa = 4.29TSFVGTFADD529 pKa = 4.09VGTQDD534 pKa = 2.75THH536 pKa = 4.44TAEE539 pKa = 4.37WNWDD543 pKa = 3.58DD544 pKa = 5.28SSTSDD549 pKa = 3.58GTVTEE554 pKa = 4.47SNGLGKK560 pKa = 10.65VSGSHH565 pKa = 5.41TFSSVGEE572 pKa = 3.95YY573 pKa = 10.32TITLTVTDD581 pKa = 5.44DD582 pKa = 3.45DD583 pKa = 4.36TGADD587 pKa = 3.45SSTKK591 pKa = 9.5TICVVDD597 pKa = 3.62AAEE600 pKa = 5.05ALTITNSYY608 pKa = 9.45IQGLSDD614 pKa = 4.74DD615 pKa = 4.29AFKK618 pKa = 11.35SKK620 pKa = 10.95ADD622 pKa = 3.57QRR624 pKa = 11.84KK625 pKa = 8.79KK626 pKa = 10.99AFDD629 pKa = 3.05KK630 pKa = 10.57MFNVLQDD637 pKa = 3.6RR638 pKa = 11.84LADD641 pKa = 3.37EE642 pKa = 5.52HH643 pKa = 6.63YY644 pKa = 10.64RR645 pKa = 11.84AMIQIMRR652 pKa = 11.84NNIRR656 pKa = 11.84AKK658 pKa = 10.76ADD660 pKa = 3.23GSVDD664 pKa = 3.3GKK666 pKa = 11.47VNNDD670 pKa = 3.72WIIDD674 pKa = 3.81TVAQEE679 pKa = 4.43HH680 pKa = 5.84VCQKK684 pKa = 10.11IDD686 pKa = 4.41DD687 pKa = 3.69ITRR690 pKa = 11.84YY691 pKa = 10.19LEE693 pKa = 3.91YY694 pKa = 10.48LIKK697 pKa = 10.73SS698 pKa = 3.68

Molecular weight: 77.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A133VLD5|A0A133VLD5_9EURY Uncharacterized protein OS=candidate division MSBL1 archaeon SCGC-AAA382C18 OX=1698281 GN=AKJ52_00675 PE=4 SV=1
MM1 pKa = 7.07YY2 pKa = 10.71VLALVLSLFEE12 pKa = 4.15VGVGNKK18 pKa = 9.36SFPVFDD24 pKa = 3.87VSPKK28 pKa = 9.16YY29 pKa = 10.72VGWLLRR35 pKa = 11.84IFLPKK40 pKa = 10.11NVSLMRR46 pKa = 11.84ILSKK50 pKa = 10.64LRR52 pKa = 11.84SARR55 pKa = 11.84DD56 pKa = 3.23FRR58 pKa = 11.84GEE60 pKa = 3.72LRR62 pKa = 11.84GVSEE66 pKa = 4.62IGPPAEE72 pKa = 4.5GKK74 pKa = 10.18RR75 pKa = 11.84SPRR78 pKa = 11.84GLFRR82 pKa = 11.84VLGRR86 pKa = 11.84AFGVAKK92 pKa = 10.68QNDD95 pKa = 5.05LILMVV100 pKa = 4.29

Molecular weight: 11.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

551

0

551

131962

43

955

239.5

27.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.514 ± 0.103

0.907 ± 0.039

6.081 ± 0.107

10.054 ± 0.212

4.075 ± 0.092

7.088 ± 0.114

1.672 ± 0.044

7.529 ± 0.12

7.91 ± 0.127

9.276 ± 0.139

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.234 ± 0.047

4.135 ± 0.077

3.91 ± 0.071

2.302 ± 0.049

5.173 ± 0.094

6.616 ± 0.09

4.721 ± 0.073

6.56 ± 0.096

1.09 ± 0.038

3.152 ± 0.062
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski