Corvus monedula polyomavirus 1
Average proteome isoelectric point is 6.16
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q20HY6|Q20HY6_9POLY Putative ORF-X OS=Corvus monedula polyomavirus 1 OX=1891748 PE=4 SV=1
MM1 pKa = 6.8 EE2 pKa = 4.94 TPMEE6 pKa = 4.14 TDD8 pKa = 3.57 AGPGATPVGSEE19 pKa = 4.34 TSNSSPQPPTPGRR32 pKa = 11.84 SPGAPQEE39 pKa = 4.09 QPAAEE44 pKa = 4.78 GGNTDD49 pKa = 4.2 HH50 pKa = 7.22 GATPTATPSSSEE62 pKa = 3.92 TTSRR66 pKa = 11.84 PKK68 pKa = 10.72 ADD70 pKa = 3.47 AVSTSTDD77 pKa = 2.58 GRR79 pKa = 11.84 TTSEE83 pKa = 3.99 TLTPQSSPSNIDD95 pKa = 3.62 SPPSSPDD102 pKa = 3.24 LHH104 pKa = 7.02 DD105 pKa = 5.16 LPAPSYY111 pKa = 10.36 EE112 pKa = 4.34 GSCTCTFSLRR122 pKa = 11.84 ASYY125 pKa = 10.63 DD126 pKa = 3.51 GRR128 pKa = 11.84 DD129 pKa = 3.07 HH130 pKa = 7.59 SYY132 pKa = 10.82 RR133 pKa = 11.84 PRR135 pKa = 11.84 NPPEE139 pKa = 3.96 TPARR143 pKa = 11.84 TSRR146 pKa = 11.84 SRR148 pKa = 11.84 ATT150 pKa = 3.33
Molecular weight: 15.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.775
IPC2_protein 4.813
IPC_protein 4.685
Toseland 4.558
ProMoST 4.813
Dawson 4.66
Bjellqvist 4.8
Wikipedia 4.533
Rodwell 4.546
Grimsley 4.482
Solomon 4.647
Lehninger 4.609
Nozaki 4.774
DTASelect 4.94
Thurlkill 4.571
EMBOSS 4.558
Sillero 4.825
Patrickios 3.961
IPC_peptide 4.66
IPC2_peptide 4.813
IPC2.peptide.svr19 4.804
Protein with the highest isoelectric point:
>tr|Q20HY5|Q20HY5_9POLY Minor capsid protein OS=Corvus monedula polyomavirus 1 OX=1891748 PE=3 SV=1
MM1 pKa = 7.69 ALQVWTPNIDD11 pKa = 3.51 YY12 pKa = 9.87 LFPGVNSFVDD22 pKa = 3.5 ALYY25 pKa = 10.56 HH26 pKa = 6.18 VNPLDD31 pKa = 3.44 WGPSLFRR38 pKa = 11.84 DD39 pKa = 4.19 LGQQIWDD46 pKa = 4.67 FIIQTGRR53 pKa = 11.84 RR54 pKa = 11.84 HH55 pKa = 6.4 LGDD58 pKa = 3.19 ATRR61 pKa = 11.84 AAVEE65 pKa = 4.18 QSASTLFDD73 pKa = 3.82 LLARR77 pKa = 11.84 ATEE80 pKa = 4.06 SATWYY85 pKa = 10.04 VVEE88 pKa = 4.97 TPVSTYY94 pKa = 10.91 RR95 pKa = 11.84 FLEE98 pKa = 4.36 DD99 pKa = 3.93 YY100 pKa = 10.53 YY101 pKa = 10.96 RR102 pKa = 11.84 QAPILRR108 pKa = 11.84 PDD110 pKa = 3.15 ARR112 pKa = 11.84 RR113 pKa = 11.84 GLAIGMRR120 pKa = 11.84 DD121 pKa = 4.58 DD122 pKa = 3.47 ILEE125 pKa = 5.06 PIPVPQRR132 pKa = 11.84 LEE134 pKa = 3.97 KK135 pKa = 10.69 VAEE138 pKa = 4.23 FPKK141 pKa = 10.74 LEE143 pKa = 4.24 EE144 pKa = 4.14 PSGQKK149 pKa = 9.36 VAPTLAPGGAHH160 pKa = 6.19 QRR162 pKa = 11.84 SCPDD166 pKa = 2.51 WMLPLILGLYY176 pKa = 7.72 GTVFPGWKK184 pKa = 10.21 AEE186 pKa = 4.01 VQLLEE191 pKa = 4.28 NQEE194 pKa = 4.54 HH195 pKa = 6.75 GPQKK199 pKa = 8.85 TPRR202 pKa = 11.84 RR203 pKa = 11.84 RR204 pKa = 11.84 TLPRR208 pKa = 11.84 SQAPYY213 pKa = 9.37 QGRR216 pKa = 11.84 HH217 pKa = 4.6 RR218 pKa = 11.84 SLRR221 pKa = 11.84 SKK223 pKa = 10.6 NRR225 pKa = 11.84 SRR227 pKa = 4.06
Molecular weight: 25.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.79
IPC2_protein 8.741
IPC_protein 8.916
Toseland 9.136
ProMoST 9.268
Dawson 9.472
Bjellqvist 9.297
Wikipedia 9.721
Rodwell 9.531
Grimsley 9.589
Solomon 9.589
Lehninger 9.531
Nozaki 9.209
DTASelect 9.268
Thurlkill 9.282
EMBOSS 9.575
Sillero 9.428
Patrickios 4.8
IPC_peptide 9.575
IPC2_peptide 8.112
IPC2.peptide.svr19 7.814
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1865
150
636
310.8
34.5
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.15 ± 1.227
1.984 ± 0.767
5.362 ± 0.335
6.488 ± 0.499
3.217 ± 0.406
6.917 ± 1.023
1.93 ± 0.193
4.129 ± 0.459
4.879 ± 1.157
10.188 ± 0.78
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.306 ± 0.399
3.378 ± 0.589
7.775 ± 1.304
4.397 ± 0.398
6.327 ± 0.635
6.273 ± 0.996
6.81 ± 0.88
5.04 ± 0.524
0.912 ± 0.387
3.539 ± 0.391
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here