Gordonia phage GAL1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Galunavirus; Gordonia virus GAL1

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 82 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A159B6G5|A0A159B6G5_9CAUD Uncharacterized protein OS=Gordonia phage GAL1 OX=1647469 GN=GAL1_79 PE=4 SV=1
MM1 pKa = 8.5RR2 pKa = 11.84MMCFINPDD10 pKa = 2.83GSKK13 pKa = 10.41RR14 pKa = 11.84YY15 pKa = 9.86VNPLQVSVIEE25 pKa = 4.21KK26 pKa = 9.55TDD28 pKa = 3.59DD29 pKa = 3.4GGVRR33 pKa = 11.84MIYY36 pKa = 9.92PGGYY40 pKa = 9.93VLFPDD45 pKa = 4.85GDD47 pKa = 3.76IDD49 pKa = 4.1QLASQWFHH57 pKa = 6.14SVNAVIDD64 pKa = 4.15PSEE67 pKa = 4.52LEE69 pKa = 4.06APP71 pKa = 4.01

Molecular weight: 7.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A159B6E9|A0A159B6E9_9CAUD Uncharacterized protein OS=Gordonia phage GAL1 OX=1647469 GN=GAL1_54 PE=4 SV=1
MM1 pKa = 7.1STGFHH6 pKa = 6.41IEE8 pKa = 4.12YY9 pKa = 10.23VPSRR13 pKa = 11.84NMDD16 pKa = 3.0RR17 pKa = 11.84RR18 pKa = 11.84SDD20 pKa = 3.46RR21 pKa = 11.84NTIDD25 pKa = 4.13DD26 pKa = 3.8KK27 pKa = 11.25KK28 pKa = 10.41QAVGSSSVIPGSNLLNRR45 pKa = 11.84NRR47 pKa = 11.84NVSEE51 pKa = 4.11MLADD55 pKa = 4.26LPKK58 pKa = 10.32PPAWHH63 pKa = 6.56EE64 pKa = 3.91LGICRR69 pKa = 11.84QFDD72 pKa = 3.64PEE74 pKa = 3.94IFYY77 pKa = 10.11PSHH80 pKa = 6.42YY81 pKa = 10.67GPTQTADD88 pKa = 3.26AKK90 pKa = 10.4KK91 pKa = 9.88VCAGCDD97 pKa = 3.35VRR99 pKa = 11.84AVCLQWALDD108 pKa = 3.7NNDD111 pKa = 3.0QHH113 pKa = 6.89GVLGGTTPRR122 pKa = 11.84EE123 pKa = 4.39SEE125 pKa = 3.99SHH127 pKa = 5.56GQGGCGMTCQCGRR140 pKa = 11.84PAHH143 pKa = 6.1ARR145 pKa = 11.84GRR147 pKa = 11.84CARR150 pKa = 11.84CYY152 pKa = 9.25AQLRR156 pKa = 11.84ARR158 pKa = 11.84QTAYY162 pKa = 10.53GRR164 pKa = 11.84WEE166 pKa = 3.92PSYY169 pKa = 11.38TDD171 pKa = 3.34AQPVRR176 pKa = 11.84EE177 pKa = 4.38HH178 pKa = 6.77LLALRR183 pKa = 11.84AAGIGNRR190 pKa = 11.84KK191 pKa = 8.6VRR193 pKa = 11.84EE194 pKa = 4.13LTVIAPSTIQAILYY208 pKa = 7.29GRR210 pKa = 11.84PARR213 pKa = 11.84GNPPARR219 pKa = 11.84KK220 pKa = 9.34VLRR223 pKa = 11.84NTAARR228 pKa = 11.84VLAIPVPVSKK238 pKa = 10.82VPCLRR243 pKa = 11.84GGRR246 pKa = 11.84VVPALGSVRR255 pKa = 11.84RR256 pKa = 11.84LQALAANGYY265 pKa = 7.09SQRR268 pKa = 11.84EE269 pKa = 3.97LWSRR273 pKa = 11.84LGWPSPQNATQMFAGRR289 pKa = 11.84VEE291 pKa = 4.51NITVVRR297 pKa = 11.84AQQVTVLFSQLQMVPGTDD315 pKa = 2.72RR316 pKa = 11.84NARR319 pKa = 11.84ARR321 pKa = 11.84AKK323 pKa = 10.69AKK325 pKa = 10.14GWLPPLAWDD334 pKa = 4.33EE335 pKa = 4.93DD336 pKa = 4.8RR337 pKa = 11.84IDD339 pKa = 4.31DD340 pKa = 3.8PTYY343 pKa = 10.43TPEE346 pKa = 4.95LVDD349 pKa = 3.82KK350 pKa = 10.43PRR352 pKa = 11.84TAEE355 pKa = 4.04DD356 pKa = 3.56VFSDD360 pKa = 4.01FEE362 pKa = 4.44YY363 pKa = 10.69LLSMGVGVEE372 pKa = 4.02DD373 pKa = 3.8ASRR376 pKa = 11.84RR377 pKa = 11.84VGLLPASVKK386 pKa = 9.98RR387 pKa = 11.84RR388 pKa = 11.84YY389 pKa = 8.35EE390 pKa = 3.7RR391 pKa = 11.84HH392 pKa = 5.39GRR394 pKa = 11.84RR395 pKa = 11.84CPAALTAVARR405 pKa = 11.84QQRR408 pKa = 11.84KK409 pKa = 5.42TASS412 pKa = 3.14

Molecular weight: 45.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

82

0

82

15857

38

1465

193.4

21.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.187 ± 0.437

1.173 ± 0.205

6.685 ± 0.287

5.651 ± 0.274

2.882 ± 0.173

8.186 ± 0.352

2.245 ± 0.249

4.257 ± 0.168

3.241 ± 0.232

7.858 ± 0.298

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.207 ± 0.143

2.964 ± 0.182

5.966 ± 0.178

3.847 ± 0.333

7.669 ± 0.437

5.531 ± 0.299

6.842 ± 0.312

7.315 ± 0.273

1.98 ± 0.137

2.314 ± 0.141
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski