Beihai weivirus-like virus 15
Average proteome isoelectric point is 8.42
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KLC4|A0A1L3KLC4_9VIRU Uncharacterized protein OS=Beihai weivirus-like virus 15 OX=1922743 PE=4 SV=1
MM1 pKa = 6.9 FTRR4 pKa = 11.84 FARR7 pKa = 11.84 DD8 pKa = 3.08 GSIRR12 pKa = 11.84 RR13 pKa = 11.84 VCAFCDD19 pKa = 3.54 RR20 pKa = 11.84 GALDD24 pKa = 4.81 AVADD28 pKa = 4.41 LDD30 pKa = 4.6 GDD32 pKa = 3.9 LCLEE36 pKa = 3.97 YY37 pKa = 10.66 DD38 pKa = 3.88 VWPDD42 pKa = 3.48 CQRR45 pKa = 11.84 CAVAYY50 pKa = 7.37 WSPFACCHH58 pKa = 6.3 PPRR61 pKa = 11.84 GPARR65 pKa = 11.84 RR66 pKa = 11.84 ILHH69 pKa = 6.26 LAVEE73 pKa = 4.39 DD74 pKa = 4.51 AEE76 pKa = 5.13 AILYY80 pKa = 9.91 NYY82 pKa = 8.92 TSASDD87 pKa = 3.66 ASLTATLVNRR97 pKa = 11.84 LRR99 pKa = 11.84 GFGCPQVSVALLRR112 pKa = 11.84 LLPPILDD119 pKa = 5.21 AIQADD124 pKa = 4.23 RR125 pKa = 11.84 PYY127 pKa = 11.09 RR128 pKa = 11.84 DD129 pKa = 4.02 ALLDD133 pKa = 3.44 MRR135 pKa = 11.84 KK136 pKa = 9.57 EE137 pKa = 4.06 DD138 pKa = 3.71 GAGVEE143 pKa = 4.42 SAQRR147 pKa = 11.84 TSCCEE152 pKa = 3.86 GLCDD156 pKa = 5.01 GAQQRR161 pKa = 11.84 PPRR164 pKa = 11.84 WWFRR168 pKa = 11.84 RR169 pKa = 11.84 PARR172 pKa = 11.84 VGQPADD178 pKa = 4.2 PGPPGPNGGGGVGVGGPEE196 pKa = 3.59 QRR198 pKa = 11.84 ANTTAANGTPNSSTTTAGPIRR219 pKa = 11.84 APGGARR225 pKa = 11.84 EE226 pKa = 4.59 GGRR229 pKa = 11.84 ADD231 pKa = 4.28 PGPGRR236 pKa = 11.84 AGGGTPPATGTSHH249 pKa = 6.98 APAGEE254 pKa = 4.32 GARR257 pKa = 11.84 STEE260 pKa = 4.09 QQQSGPARR268 pKa = 11.84 PRR270 pKa = 11.84 LCPPPGAGSLRR281 pKa = 11.84 QQDD284 pKa = 3.13 VDD286 pKa = 4.0 RR287 pKa = 11.84 GVGTDD292 pKa = 3.41 TTAPPPGRR300 pKa = 11.84 IDD302 pKa = 3.56 PPHH305 pKa = 6.5 AVGAILRR312 pKa = 11.84 SEE314 pKa = 4.1 AHH316 pKa = 6.93 PARR319 pKa = 11.84 CEE321 pKa = 3.97 TQVTVEE327 pKa = 3.85 RR328 pKa = 11.84 LEE330 pKa = 4.19 PPKK333 pKa = 10.63 EE334 pKa = 3.49 RR335 pKa = 11.84 VAISRR340 pKa = 11.84 ATNPCDD346 pKa = 3.27 PQHH349 pKa = 6.72 FDD351 pKa = 3.21 EE352 pKa = 5.32 SLTNVMAATAQRR364 pKa = 11.84 VEE366 pKa = 4.22 RR367 pKa = 11.84 YY368 pKa = 8.34 QPKK371 pKa = 10.0 FKK373 pKa = 10.5 PGPQLRR379 pKa = 11.84 ARR381 pKa = 11.84 LEE383 pKa = 3.96 KK384 pKa = 10.9 VRR386 pKa = 11.84 DD387 pKa = 3.89 SIIEE391 pKa = 3.94 RR392 pKa = 11.84 VLTKK396 pKa = 10.61 KK397 pKa = 10.52 KK398 pKa = 9.75 IYY400 pKa = 9.67 QWLDD404 pKa = 3.03 EE405 pKa = 4.52 HH406 pKa = 6.34 PTLRR410 pKa = 11.84 SAMSKK415 pKa = 9.79 AWSDD419 pKa = 3.2 EE420 pKa = 3.91 RR421 pKa = 11.84 AQRR424 pKa = 11.84 AVEE427 pKa = 3.99 TLRR430 pKa = 11.84 GLGFDD435 pKa = 3.62 VRR437 pKa = 11.84 ITHH440 pKa = 6.14 EE441 pKa = 4.07 ASIKK445 pKa = 11.08 DD446 pKa = 3.45 EE447 pKa = 4.0 VLPNRR452 pKa = 11.84 GKK454 pKa = 10.61 APRR457 pKa = 11.84 LIISCGDD464 pKa = 3.36 QGQLCALIHH473 pKa = 5.06 VACFEE478 pKa = 4.05 GLLYY482 pKa = 10.99 DD483 pKa = 3.81 HH484 pKa = 7.03 FEE486 pKa = 3.99 KK487 pKa = 10.77 RR488 pKa = 11.84 SIKK491 pKa = 10.16 HH492 pKa = 5.35 RR493 pKa = 11.84 AKK495 pKa = 10.65 ADD497 pKa = 3.4 ALGEE501 pKa = 3.98 VSGRR505 pKa = 11.84 AKK507 pKa = 10.41 QEE509 pKa = 3.51 EE510 pKa = 5.07 GFVKK514 pKa = 10.36 RR515 pKa = 11.84 YY516 pKa = 10.42 LEE518 pKa = 4.44 GDD520 pKa = 3.53 GKK522 pKa = 10.96 AWDD525 pKa = 3.72 RR526 pKa = 11.84 TITPRR531 pKa = 11.84 LRR533 pKa = 11.84 EE534 pKa = 3.99 LLEE537 pKa = 4.35 NPLLEE542 pKa = 4.68 HH543 pKa = 6.31 VANTIYY549 pKa = 10.94 ARR551 pKa = 11.84 WDD553 pKa = 3.98 AIGSLSYY560 pKa = 9.34 WASVDD565 pKa = 2.88 IKK567 pKa = 10.96 DD568 pKa = 3.36 RR569 pKa = 11.84 KK570 pKa = 9.87 KK571 pKa = 9.62 PIRR574 pKa = 11.84 RR575 pKa = 11.84 LVYY578 pKa = 9.59 RR579 pKa = 11.84 KK580 pKa = 10.0 GSRR583 pKa = 11.84 AHH585 pKa = 6.04 MFLEE589 pKa = 4.54 AFRR592 pKa = 11.84 ASGDD596 pKa = 3.46 RR597 pKa = 11.84 GTSCLNWVVNYY608 pKa = 10.2 SCWLAVLVAYY618 pKa = 10.08 EE619 pKa = 4.72 DD620 pKa = 3.26 IDD622 pKa = 4.28 RR623 pKa = 11.84 AVTQPNWRR631 pKa = 11.84 WFRR634 pKa = 11.84 PAPHH638 pKa = 6.72 YY639 pKa = 10.57 LRR641 pKa = 11.84 NDD643 pKa = 2.59 KK644 pKa = 9.73 RR645 pKa = 11.84 TLSTRR650 pKa = 11.84 RR651 pKa = 11.84 KK652 pKa = 8.02 MFMDD656 pKa = 3.94 SVYY659 pKa = 10.81 EE660 pKa = 4.26 GDD662 pKa = 4.44 DD663 pKa = 3.71 SFVMISTDD671 pKa = 3.6 RR672 pKa = 11.84 PLEE675 pKa = 4.71 DD676 pKa = 5.23 IEE678 pKa = 4.94 AAWASLGFEE687 pKa = 3.92 MKK689 pKa = 10.49 LFEE692 pKa = 4.58 RR693 pKa = 11.84 RR694 pKa = 11.84 PGSVVTFVGNEE705 pKa = 3.94 CLVKK709 pKa = 10.91 EE710 pKa = 4.58 EE711 pKa = 4.49 GLSDD715 pKa = 3.6 LWLPQIARR723 pKa = 11.84 NLTAAAEE730 pKa = 4.01 QRR732 pKa = 11.84 DD733 pKa = 4.48 GGGDD737 pKa = 3.49 PHH739 pKa = 6.37 QHH741 pKa = 5.98 SLACAVRR748 pKa = 11.84 AQGYY752 pKa = 9.03 KK753 pKa = 10.17 RR754 pKa = 11.84 YY755 pKa = 9.14 PPLALHH761 pKa = 6.15 YY762 pKa = 10.31 LAIAKK767 pKa = 9.54 SCRR770 pKa = 11.84 DD771 pKa = 3.92 SNTLAQPVAKK781 pKa = 8.97 ATQYY785 pKa = 11.79 AWFGNYY791 pKa = 9.89 NPDD794 pKa = 3.13 RR795 pKa = 11.84 RR796 pKa = 11.84 LEE798 pKa = 4.12 PEE800 pKa = 4.37 VYY802 pKa = 9.87 EE803 pKa = 4.49 LPAVDD808 pKa = 3.73 YY809 pKa = 11.1 HH810 pKa = 7.13 PDD812 pKa = 3.4 AEE814 pKa = 4.48 ALVAAATGEE823 pKa = 4.14 APDD826 pKa = 4.23 RR827 pKa = 11.84 NAVDD831 pKa = 3.96 ALMTGSLAAEE841 pKa = 4.05 WDD843 pKa = 3.72 KK844 pKa = 10.91 EE845 pKa = 4.33 TLRR848 pKa = 11.84 RR849 pKa = 11.84 ILPRR853 pKa = 11.84 SWIEE857 pKa = 3.56
Molecular weight: 94.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.093
IPC2_protein 7.439
IPC_protein 7.234
Toseland 6.883
ProMoST 7.834
Dawson 7.936
Bjellqvist 8.434
Wikipedia 7.834
Rodwell 7.951
Grimsley 6.927
Solomon 7.966
Lehninger 7.98
Nozaki 8.697
DTASelect 8.097
Thurlkill 8.141
EMBOSS 8.156
Sillero 8.507
Patrickios 4.469
IPC_peptide 7.966
IPC2_peptide 7.644
IPC2.peptide.svr19 7.439
Protein with the highest isoelectric point:
>tr|A0A1L3KLC4|A0A1L3KLC4_9VIRU Uncharacterized protein OS=Beihai weivirus-like virus 15 OX=1922743 PE=4 SV=1
MM1 pKa = 7.25 WLWRR5 pKa = 11.84 ACSRR9 pKa = 11.84 DD10 pKa = 3.38 SRR12 pKa = 11.84 EE13 pKa = 3.75 MEE15 pKa = 4.04 ASVEE19 pKa = 4.13 CARR22 pKa = 11.84 FVTEE26 pKa = 4.67 APLTRR31 pKa = 11.84 WLTWMGISVWSTTCGPTASVVLWRR55 pKa = 11.84 IGLRR59 pKa = 11.84 SPAVIRR65 pKa = 11.84 HH66 pKa = 4.67 VARR69 pKa = 11.84 LVGFCTLRR77 pKa = 11.84 WKK79 pKa = 9.49 MRR81 pKa = 11.84 RR82 pKa = 11.84 LSSTTTPVRR91 pKa = 11.84 PMRR94 pKa = 11.84 ALPPPLSTAYY104 pKa = 9.88 GGSGAPRR111 pKa = 11.84 SQLRR115 pKa = 11.84 CCVCCLRR122 pKa = 11.84 SLTQSRR128 pKa = 11.84 LTVPIGMLCSTCGRR142 pKa = 11.84 RR143 pKa = 11.84 MAQAWSQRR151 pKa = 11.84 NAHH154 pKa = 6.86 LAAKK158 pKa = 9.49 DD159 pKa = 3.67 CVMEE163 pKa = 4.7 LSSDD167 pKa = 3.77 PPVGGFADD175 pKa = 3.84 LPVWVNRR182 pKa = 11.84 RR183 pKa = 11.84 ILDD186 pKa = 3.4 LRR188 pKa = 11.84 GPTEE192 pKa = 3.92 EE193 pKa = 5.38 EE194 pKa = 3.52 VWEE197 pKa = 4.16 WVVQNSAPTRR207 pKa = 11.84 RR208 pKa = 11.84 PPMGRR213 pKa = 11.84 RR214 pKa = 11.84 TRR216 pKa = 11.84 APPPPDD222 pKa = 3.91 PYY224 pKa = 11.49 GHH226 pKa = 7.38 LEE228 pKa = 4.03 GPVRR232 pKa = 11.84 VAALIRR238 pKa = 11.84 DD239 pKa = 4.01 RR240 pKa = 11.84 EE241 pKa = 4.1 EE242 pKa = 3.23 QAAARR247 pKa = 11.84 RR248 pKa = 11.84 RR249 pKa = 11.84 QPGPLMRR256 pKa = 11.84 LQEE259 pKa = 4.41 KK260 pKa = 9.86 EE261 pKa = 4.5 RR262 pKa = 11.84 EE263 pKa = 4.31 AQSSSKK269 pKa = 11.11 ADD271 pKa = 3.71 LLARR275 pKa = 11.84 GYY277 pKa = 11.29 APLRR281 pKa = 11.84 GPGAA285 pKa = 3.68
Molecular weight: 31.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.289
IPC2_protein 9.458
IPC_protein 10.73
Toseland 10.818
ProMoST 11.067
Dawson 10.862
Bjellqvist 10.774
Wikipedia 11.242
Rodwell 10.672
Grimsley 10.906
Solomon 11.213
Lehninger 11.14
Nozaki 10.862
DTASelect 10.76
Thurlkill 10.818
EMBOSS 11.272
Sillero 10.847
Patrickios 10.394
IPC_peptide 11.213
IPC2_peptide 10.335
IPC2.peptide.svr19 9.173
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
1497
285
857
499.0
54.61
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.957 ± 0.837
2.338 ± 0.656
5.144 ± 1.1
5.21 ± 0.748
2.004 ± 0.378
8.55 ± 0.948
1.937 ± 0.279
2.939 ± 0.436
3.273 ± 0.627
7.949 ± 0.722
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.071 ± 0.708
2.004 ± 0.378
7.749 ± 0.596
3.874 ± 0.494
9.686 ± 1.583
5.945 ± 0.693
6.346 ± 1.314
6.079 ± 0.393
2.338 ± 0.482
2.605 ± 0.524
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here