Sewage-associated circular DNA virus-13

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 8.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A075IZT3|A0A075IZT3_9VIRU Replication-associated protein OS=Sewage-associated circular DNA virus-13 OX=1519389 PE=3 SV=1
MM1 pKa = 7.98DD2 pKa = 5.23LPRR5 pKa = 11.84FRR7 pKa = 11.84LNNQRR12 pKa = 11.84LLCTYY17 pKa = 10.47AKK19 pKa = 10.52SDD21 pKa = 3.71LSKK24 pKa = 11.14KK25 pKa = 9.58KK26 pKa = 10.34LKK28 pKa = 10.54EE29 pKa = 3.83FFVSKK34 pKa = 10.55SPKK37 pKa = 8.77TSPKK41 pKa = 10.07VKK43 pKa = 9.79IARR46 pKa = 11.84EE47 pKa = 4.02SHH49 pKa = 6.34KK50 pKa = 11.0DD51 pKa = 3.39GSKK54 pKa = 10.47HH55 pKa = 4.0MHH57 pKa = 5.73VLVDD61 pKa = 3.88FGKK64 pKa = 10.8RR65 pKa = 11.84FDD67 pKa = 3.62STNRR71 pKa = 11.84RR72 pKa = 11.84VFDD75 pKa = 3.77FEE77 pKa = 4.27GRR79 pKa = 11.84HH80 pKa = 4.7PNIRR84 pKa = 11.84RR85 pKa = 11.84VEE87 pKa = 4.23SMIHH91 pKa = 5.58WANLLCYY98 pKa = 10.09LGKK101 pKa = 10.01EE102 pKa = 4.32DD103 pKa = 4.48KK104 pKa = 10.42KK105 pKa = 10.81CRR107 pKa = 11.84PSASVLAAAKK117 pKa = 9.5CWNAKK122 pKa = 8.92TPVEE126 pKa = 4.75AILSGAYY133 pKa = 9.31KK134 pKa = 10.67DD135 pKa = 4.61PIRR138 pKa = 11.84SLAVFRR144 pKa = 11.84QRR146 pKa = 11.84PSARR150 pKa = 11.84EE151 pKa = 3.92FICNMTEE158 pKa = 4.99PEE160 pKa = 4.24APWYY164 pKa = 9.84QDD166 pKa = 2.45IVKK169 pKa = 9.68PLIEE173 pKa = 4.86ANSDD177 pKa = 2.91HH178 pKa = 7.18RR179 pKa = 11.84SWYY182 pKa = 8.26WFCDD186 pKa = 3.16KK187 pKa = 11.03VGNSWKK193 pKa = 10.68SYY195 pKa = 5.69TCKK198 pKa = 10.71YY199 pKa = 10.21LLQTMRR205 pKa = 11.84EE206 pKa = 4.2DD207 pKa = 3.83FRR209 pKa = 11.84ATKK212 pKa = 9.14LTANVDD218 pKa = 3.68SFAMKK223 pKa = 10.31LGNWQQEE230 pKa = 4.09GWSGKK235 pKa = 9.8ILFVDD240 pKa = 4.54LARR243 pKa = 11.84GKK245 pKa = 10.98ANTNTIYY252 pKa = 10.78EE253 pKa = 4.42CLEE256 pKa = 4.02LTSDD260 pKa = 3.2GWGDD264 pKa = 3.25TGKK267 pKa = 10.79YY268 pKa = 8.49EE269 pKa = 4.35GKK271 pKa = 10.46SFILDD276 pKa = 3.44SSPIVVVLANWWPRR290 pKa = 11.84IAADD294 pKa = 3.62TGSLDD299 pKa = 2.95RR300 pKa = 11.84WKK302 pKa = 10.86LFEE305 pKa = 4.37ITNEE309 pKa = 4.29PIRR312 pKa = 11.84GSARR316 pKa = 11.84CMNLNIVRR324 pKa = 11.84TEE326 pKa = 3.85RR327 pKa = 11.84TDD329 pKa = 3.55LDD331 pKa = 3.83EE332 pKa = 4.61EE333 pKa = 4.23LAIQKK338 pKa = 9.39EE339 pKa = 4.42VARR342 pKa = 11.84LEE344 pKa = 4.33KK345 pKa = 10.46QMKK348 pKa = 9.27AAEE351 pKa = 4.13AVEE354 pKa = 4.09KK355 pKa = 10.89AKK357 pKa = 10.95ARR359 pKa = 11.84IAASRR364 pKa = 11.84DD365 pKa = 3.53CC366 pKa = 5.03

Molecular weight: 42.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A075IZT3|A0A075IZT3_9VIRU Replication-associated protein OS=Sewage-associated circular DNA virus-13 OX=1519389 PE=3 SV=1
MM1 pKa = 6.76STKK4 pKa = 10.24KK5 pKa = 9.31RR6 pKa = 11.84TAVPARR12 pKa = 11.84KK13 pKa = 7.38TVAAPKK19 pKa = 8.84RR20 pKa = 11.84TYY22 pKa = 11.1AKK24 pKa = 8.92RR25 pKa = 11.84TYY27 pKa = 7.88TARR30 pKa = 11.84APAATRR36 pKa = 11.84YY37 pKa = 9.79RR38 pKa = 11.84GTGDD42 pKa = 3.31YY43 pKa = 10.82VKK45 pKa = 10.35APRR48 pKa = 11.84AIRR51 pKa = 11.84PSKK54 pKa = 10.26STTKK58 pKa = 10.73SLGGTIGAHH67 pKa = 6.52LGHH70 pKa = 7.14GLQTVIKK77 pKa = 9.03ALTGFGDD84 pKa = 4.16YY85 pKa = 10.26EE86 pKa = 4.37VEE88 pKa = 4.64SNSLMPGTLGGDD100 pKa = 3.31PPIIQNSRR108 pKa = 11.84NNSHH112 pKa = 5.81VVHH115 pKa = 6.13HH116 pKa = 6.81RR117 pKa = 11.84EE118 pKa = 4.18YY119 pKa = 10.63IGDD122 pKa = 4.03VYY124 pKa = 11.41ASTAFSIQTYY134 pKa = 9.07PLNPGLLSSFPWVSQQADD152 pKa = 3.33SYY154 pKa = 9.87EE155 pKa = 4.03QYY157 pKa = 10.88KK158 pKa = 10.21FRR160 pKa = 11.84GAVFEE165 pKa = 4.31FKK167 pKa = 10.93SMSSDD172 pKa = 3.28AVLSSAASSSLGTVIMSTQYY192 pKa = 11.19NALEE196 pKa = 4.49APFSDD201 pKa = 3.04KK202 pKa = 10.37RR203 pKa = 11.84TMEE206 pKa = 3.93NYY208 pKa = 10.07EE209 pKa = 4.08YY210 pKa = 11.19ANSAKK215 pKa = 9.84PSCSMLHH222 pKa = 6.55PIEE225 pKa = 5.28CKK227 pKa = 9.59MSQTTVSEE235 pKa = 4.54LYY237 pKa = 10.24VRR239 pKa = 11.84TGVVSQGDD247 pKa = 3.83LRR249 pKa = 11.84LFDD252 pKa = 5.0LGNFSIAVQGKK263 pKa = 7.98QNVGANQVIGEE274 pKa = 4.33LGVV277 pKa = 3.23

Molecular weight: 29.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

643

277

366

321.5

36.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.02 ± 0.225

1.866 ± 0.703

4.821 ± 0.966

5.443 ± 0.904

3.733 ± 0.297

6.065 ± 1.375

1.866 ± 0.184

4.51 ± 0.331

7.621 ± 1.355

7.465 ± 0.594

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.333 ± 0.119

4.355 ± 0.236

4.355 ± 0.43

3.11 ± 0.751

6.687 ± 0.782

9.02 ± 1.334

6.065 ± 1.153

6.065 ± 0.71

2.022 ± 1.02

3.577 ± 0.908
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski