Macaca mulatta feces associated virus 7
Average proteome isoelectric point is 6.34
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1W5PZM4|A0A1W5PZM4_9VIRU Rep OS=Macaca mulatta feces associated virus 7 OX=2499229 PE=4 SV=1
MM1 pKa = 7.16 IMKK4 pKa = 9.91 VKK6 pKa = 9.17 FTQFFDD12 pKa = 3.15 ISSDD16 pKa = 3.3 STKK19 pKa = 10.19 FSKK22 pKa = 9.84 FTVTAGGDD30 pKa = 3.78 YY31 pKa = 11.21 VKK33 pKa = 10.05 MRR35 pKa = 11.84 CAPQFVCYY43 pKa = 9.94 KK44 pKa = 9.13 YY45 pKa = 10.79 FKK47 pKa = 10.34 LGPVSVKK54 pKa = 10.28 LVPASTLPVDD64 pKa = 3.87 PTGLSYY70 pKa = 9.86 EE71 pKa = 4.42 TGEE74 pKa = 4.39 STVDD78 pKa = 3.19 PRR80 pKa = 11.84 DD81 pKa = 3.38 MFNPGLVRR89 pKa = 11.84 ITNGEE94 pKa = 3.87 PVGDD98 pKa = 4.77 FNASDD103 pKa = 3.49 MNQYY107 pKa = 10.5 YY108 pKa = 8.07 ATLLDD113 pKa = 3.72 PRR115 pKa = 11.84 WFKK118 pKa = 10.86 FQLQQGFSRR127 pKa = 11.84 YY128 pKa = 9.57 AKK130 pKa = 9.5 PLVYY134 pKa = 10.6 NIGEE138 pKa = 4.18 IAQEE142 pKa = 4.03 PFPFAGTHH150 pKa = 5.46 ISVSNNVSPEE160 pKa = 3.84 YY161 pKa = 10.6 YY162 pKa = 9.66 YY163 pKa = 10.59 QQSDD167 pKa = 3.74 GSFKK171 pKa = 11.0 SGGSAADD178 pKa = 3.38 TEE180 pKa = 4.93 RR181 pKa = 11.84 YY182 pKa = 9.56 IDD184 pKa = 3.5 THH186 pKa = 6.35 TPLQTGRR193 pKa = 11.84 VKK195 pKa = 10.73 LGWMPTDD202 pKa = 3.35 SVMKK206 pKa = 9.69 MATAAVNGVNTFSPNAAAYY225 pKa = 10.05 VPEE228 pKa = 5.5 IDD230 pKa = 5.5 LITFILPKK238 pKa = 10.39 AFKK241 pKa = 9.56 TKK243 pKa = 9.26 FFYY246 pKa = 10.5 RR247 pKa = 11.84 VFVEE251 pKa = 3.88 STVYY255 pKa = 10.27 FKK257 pKa = 11.32 EE258 pKa = 4.09 PVAVNPIIEE267 pKa = 4.63 RR268 pKa = 11.84 DD269 pKa = 3.6 TDD271 pKa = 3.41 KK272 pKa = 11.38 SLYY275 pKa = 10.53 AYY277 pKa = 10.4 LPLDD281 pKa = 4.08 RR282 pKa = 11.84 GVGMQIKK289 pKa = 10.16 DD290 pKa = 2.98 IRR292 pKa = 11.84 SYY294 pKa = 11.57 TNAQKK299 pKa = 10.53 IPLGDD304 pKa = 3.43 SLGYY308 pKa = 9.73 HH309 pKa = 6.14 SKK311 pKa = 10.54 VDD313 pKa = 3.82 SVTGGAEE320 pKa = 3.82 EE321 pKa = 4.01
Molecular weight: 35.78 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.894
IPC2_protein 6.033
IPC_protein 6.122
Toseland 6.402
ProMoST 6.071
Dawson 6.389
Bjellqvist 6.338
Wikipedia 6.389
Rodwell 6.364
Grimsley 6.504
Solomon 6.389
Lehninger 6.389
Nozaki 6.605
DTASelect 6.81
Thurlkill 6.795
EMBOSS 6.795
Sillero 6.737
Patrickios 3.935
IPC_peptide 6.415
IPC2_peptide 6.517
IPC2.peptide.svr19 6.744
Protein with the highest isoelectric point:
>tr|A0A1W5PZM4|A0A1W5PZM4_9VIRU Rep OS=Macaca mulatta feces associated virus 7 OX=2499229 PE=4 SV=1
MM1 pKa = 7.22 TNKK4 pKa = 9.64 IYY6 pKa = 10.18 MITMPKK12 pKa = 9.9 NGRR15 pKa = 11.84 NKK17 pKa = 10.4 YY18 pKa = 9.57 LLKK21 pKa = 10.92 NLIEE25 pKa = 4.45 TNDD28 pKa = 3.57 LHH30 pKa = 6.73 KK31 pKa = 10.07 WAYY34 pKa = 9.99 GYY36 pKa = 10.55 EE37 pKa = 3.97 IGKK40 pKa = 9.59 NGYY43 pKa = 8.48 KK44 pKa = 10.22 HH45 pKa = 4.57 IQARR49 pKa = 11.84 IRR51 pKa = 11.84 CPFDD55 pKa = 3.77 FDD57 pKa = 4.0 YY58 pKa = 11.1 LQEE61 pKa = 4.3 YY62 pKa = 9.5 FGQAHH67 pKa = 6.49 IEE69 pKa = 4.09 EE70 pKa = 5.36 ASDD73 pKa = 2.99 EE74 pKa = 4.01 WDD76 pKa = 3.51 YY77 pKa = 10.24 EE78 pKa = 4.68 TKK80 pKa = 10.72 GGIYY84 pKa = 8.13 FTSEE88 pKa = 3.37 DD89 pKa = 2.84 WGEE92 pKa = 4.01 RR93 pKa = 11.84 LKK95 pKa = 10.81 QRR97 pKa = 11.84 YY98 pKa = 8.46 LPLKK102 pKa = 9.36 EE103 pKa = 4.29 VQNKK107 pKa = 9.52 ALEE110 pKa = 4.35 GLEE113 pKa = 3.97 ATNDD117 pKa = 3.36 RR118 pKa = 11.84 EE119 pKa = 4.33 VYY121 pKa = 9.31 VWYY124 pKa = 9.85 DD125 pKa = 3.32 EE126 pKa = 4.51 KK127 pKa = 11.79 GNAGKK132 pKa = 10.08 SWLCGHH138 pKa = 6.98 LWEE141 pKa = 4.71 TGKK144 pKa = 10.88 AYY146 pKa = 10.72 VVDD149 pKa = 3.94 SDD151 pKa = 4.01 KK152 pKa = 11.89 GEE154 pKa = 4.27 TIKK157 pKa = 10.89 KK158 pKa = 10.08 DD159 pKa = 3.39 VANEE163 pKa = 4.12 YY164 pKa = 10.09 INHH167 pKa = 5.53 GWRR170 pKa = 11.84 PYY172 pKa = 10.95 VIIDD176 pKa = 4.03 LPRR179 pKa = 11.84 TAKK182 pKa = 8.39 WTDD185 pKa = 3.06 EE186 pKa = 4.05 LYY188 pKa = 11.12 YY189 pKa = 10.63 AIEE192 pKa = 4.52 KK193 pKa = 10.47 IKK195 pKa = 10.97 DD196 pKa = 3.62 GLLKK200 pKa = 10.6 DD201 pKa = 3.9 PRR203 pKa = 11.84 YY204 pKa = 9.68 QSKK207 pKa = 7.83 TVNIHH212 pKa = 4.58 GVKK215 pKa = 10.24 VMVMCNHH222 pKa = 6.92 RR223 pKa = 11.84 PNVSKK228 pKa = 11.02 LSADD232 pKa = 3.22 RR233 pKa = 11.84 WKK235 pKa = 10.43 MFTGAAAPEE244 pKa = 4.2 PPAMQTAGGLSRR256 pKa = 11.84 SSS258 pKa = 3.15
Molecular weight: 29.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.782
IPC2_protein 6.971
IPC_protein 7.176
Toseland 7.117
ProMoST 7.424
Dawson 7.79
Bjellqvist 7.673
Wikipedia 7.746
Rodwell 7.775
Grimsley 7.205
Solomon 7.922
Lehninger 7.922
Nozaki 7.878
DTASelect 7.863
Thurlkill 7.907
EMBOSS 8.039
Sillero 8.112
Patrickios 4.126
IPC_peptide 7.922
IPC2_peptide 7.102
IPC2.peptide.svr19 7.431
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
579
258
321
289.5
32.88
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.563 ± 0.017
0.864 ± 0.191
6.218 ± 0.01
5.699 ± 1.309
4.663 ± 1.739
7.427 ± 0.208
1.727 ± 0.629
5.181 ± 0.404
7.945 ± 1.113
6.218 ± 0.484
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.763 ± 0.032
4.491 ± 0.597
5.527 ± 1.053
3.282 ± 0.363
3.972 ± 0.433
6.045 ± 1.384
6.39 ± 0.862
6.736 ± 1.33
1.9 ± 1.013
6.39 ± 0.374
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here