Streptococcus satellite phage Javan545

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 17 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZR27|A0A4D5ZR27_9VIRU Putative transcription regulator OS=Streptococcus satellite phage Javan545 OX=2558739 GN=JavanS545_0005 PE=4 SV=1
MM1 pKa = 7.82AKK3 pKa = 7.93TTEE6 pKa = 4.0QTKK9 pKa = 8.33TVQLTVEE16 pKa = 4.22EE17 pKa = 4.34LQGLGYY23 pKa = 10.52RR24 pKa = 11.84LSNILKK30 pKa = 8.93TIKK33 pKa = 10.18LDD35 pKa = 3.53QVAQAGVSLSKK46 pKa = 10.52DD47 pKa = 2.8WEE49 pKa = 4.44SFIFTDD55 pKa = 3.56IATSYY60 pKa = 10.57LSSSYY65 pKa = 10.86EE66 pKa = 3.8VFEE69 pKa = 4.5TIIAEE74 pKa = 4.32LDD76 pKa = 4.33DD77 pKa = 4.17IASQLLEE84 pKa = 4.78CDD86 pKa = 3.55DD87 pKa = 4.73AEE89 pKa = 4.16EE90 pKa = 5.29LEE92 pKa = 4.39GFRR95 pKa = 11.84NGRR98 pKa = 3.5

Molecular weight: 10.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZVB6|A0A4D5ZVB6_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan545 OX=2558739 GN=JavanS545_0007 PE=4 SV=1
MM1 pKa = 7.45FSLSKK6 pKa = 10.65EE7 pKa = 4.34SEE9 pKa = 3.82NSLNQGILKK18 pKa = 10.21LINDD22 pKa = 4.28FLKK25 pKa = 10.81VSKK28 pKa = 10.33QPKK31 pKa = 8.43PRR33 pKa = 11.84QLGLMTPRR41 pKa = 11.84QIKK44 pKa = 10.15EE45 pKa = 3.88EE46 pKa = 4.11LGITARR52 pKa = 11.84TLQRR56 pKa = 11.84WEE58 pKa = 3.69RR59 pKa = 11.84AGLRR63 pKa = 11.84RR64 pKa = 11.84YY65 pKa = 8.78QPPVEE70 pKa = 4.25DD71 pKa = 3.6TRR73 pKa = 11.84KK74 pKa = 9.28VFYY77 pKa = 10.12RR78 pKa = 11.84VSDD81 pKa = 3.24ILIFLGVDD89 pKa = 3.0NGG91 pKa = 3.71

Molecular weight: 10.57 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

17

0

17

2788

44

381

164.0

18.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.313 ± 0.46

0.61 ± 0.144

5.595 ± 0.413

9.469 ± 0.727

4.089 ± 0.413

4.34 ± 0.247

1.722 ± 0.269

7.532 ± 0.36

9.971 ± 0.589

9.326 ± 0.46

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.439 ± 0.261

5.488 ± 0.472

2.403 ± 0.301

4.663 ± 0.445

5.093 ± 0.362

5.99 ± 0.305

5.667 ± 0.585

4.34 ± 0.426

0.681 ± 0.126

4.268 ± 0.487
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski