Atelocyanobacterium thalassa (isolate ALOHA)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Oscillatoriophycideae; Chroococcales; Aphanothecaceae; Candidatus Atelocyanobacterium; Candidatus Atelocyanobacterium thalassa

Average proteome isoelectric point is 7.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1199 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B7TB22|B7TB22_ATETH Uncharacterized protein OS=Atelocyanobacterium thalassa (isolate ALOHA) OX=1453429 GN=UCYN_06280 PE=4 SV=1
MM1 pKa = 7.26ATYY4 pKa = 10.62KK5 pKa = 9.47VTLTNEE11 pKa = 3.94EE12 pKa = 3.97EE13 pKa = 5.12GINSTLEE20 pKa = 4.0VPEE23 pKa = 4.88DD24 pKa = 3.91EE25 pKa = 5.23YY26 pKa = 11.16ILDD29 pKa = 3.7VAEE32 pKa = 4.36EE33 pKa = 4.23EE34 pKa = 4.4GLEE37 pKa = 4.2IPFSCRR43 pKa = 11.84AGACSTCAGKK53 pKa = 10.35LLSGSVDD60 pKa = 3.27QSDD63 pKa = 4.14QSFLDD68 pKa = 3.95DD69 pKa = 4.25DD70 pKa = 4.09QIEE73 pKa = 4.27AGFVLTCVAYY83 pKa = 7.25PTSDD87 pKa = 3.65CVIMTHH93 pKa = 6.32QEE95 pKa = 3.61EE96 pKa = 4.23TLYY99 pKa = 11.3

Molecular weight: 10.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D3ENM9|D3ENM9_ATETH Uncharacterized membrane protein OS=Atelocyanobacterium thalassa (isolate ALOHA) OX=1453429 GN=UCYN_03380 PE=4 SV=1
MM1 pKa = 6.61TQRR4 pKa = 11.84TLGGTNRR11 pKa = 11.84KK12 pKa = 7.61QKK14 pKa = 8.99RR15 pKa = 11.84TSGFRR20 pKa = 11.84ARR22 pKa = 11.84MRR24 pKa = 11.84DD25 pKa = 3.25HH26 pKa = 7.22NGRR29 pKa = 11.84KK30 pKa = 9.26VIQARR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.86RR38 pKa = 11.84GRR40 pKa = 11.84HH41 pKa = 4.78RR42 pKa = 11.84LSVV45 pKa = 3.19

Molecular weight: 5.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1199

0

1199

386876

22

1982

322.7

36.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.815 ± 0.07

1.048 ± 0.023

4.627 ± 0.052

6.177 ± 0.071

4.335 ± 0.057

6.212 ± 0.064

1.847 ± 0.03

9.233 ± 0.063

7.026 ± 0.073

11.089 ± 0.081

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.905 ± 0.032

5.545 ± 0.07

3.872 ± 0.038

4.138 ± 0.043

4.213 ± 0.055

7.136 ± 0.055

5.282 ± 0.038

5.853 ± 0.058

1.237 ± 0.031

3.41 ± 0.043
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski