Staphylococcus virus phi12

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Triavirus

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|Q8SDR4|Q8SDR4_9CAUD Uncharacterized protein OS=Staphylococcus virus phi12 OX=186152 PE=4 SV=1
MM1 pKa = 7.06ATQRR5 pKa = 11.84QVEE8 pKa = 4.5YY9 pKa = 11.4VMSLQEE15 pKa = 3.92QLEE18 pKa = 4.35LEE20 pKa = 4.45DD21 pKa = 3.95CEE23 pKa = 5.56KK24 pKa = 10.67YY25 pKa = 9.42TDD27 pKa = 3.83EE28 pKa = 4.01QVKK31 pKa = 10.4AMSHH35 pKa = 6.03KK36 pKa = 10.06EE37 pKa = 3.72VSNVIEE43 pKa = 4.41NYY45 pKa = 9.42KK46 pKa = 10.77ASISNEE52 pKa = 3.8EE53 pKa = 4.63LYY55 pKa = 10.85DD56 pKa = 3.48EE57 pKa = 4.93CMSFGLPNCC66 pKa = 4.51

Molecular weight:
7.7 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|Q8SDR6|Q8SDR6_9CAUD ETA orf 26-like protein OS=Staphylococcus virus phi12 OX=186152 PE=4 SV=1
MM1 pKa = 6.5MARR4 pKa = 11.84RR5 pKa = 11.84KK6 pKa = 9.8VIRR9 pKa = 11.84VRR11 pKa = 11.84IKK13 pKa = 10.5GKK15 pKa = 10.6LMTLRR20 pKa = 11.84EE21 pKa = 4.12VSEE24 pKa = 4.8KK25 pKa = 10.69YY26 pKa = 10.32HH27 pKa = 6.64ISPEE31 pKa = 4.02LLRR34 pKa = 11.84YY35 pKa = 9.46RR36 pKa = 11.84YY37 pKa = 8.24KK38 pKa = 10.75HH39 pKa = 6.21KK40 pKa = 10.45MRR42 pKa = 11.84GDD44 pKa = 3.41EE45 pKa = 4.19LLCGRR50 pKa = 11.84KK51 pKa = 9.42DD52 pKa = 3.5SKK54 pKa = 11.27SKK56 pKa = 11.42EE57 pKa = 3.71EE58 pKa = 3.88VEE60 pKa = 4.3YY61 pKa = 10.09MKK63 pKa = 10.92SQIKK67 pKa = 10.48DD68 pKa = 3.39EE69 pKa = 4.37EE70 pKa = 4.21KK71 pKa = 10.52GRR73 pKa = 11.84EE74 pKa = 4.07KK75 pKa = 10.34IRR77 pKa = 11.84KK78 pKa = 8.34KK79 pKa = 10.82AILNRR84 pKa = 11.84YY85 pKa = 5.97QRR87 pKa = 11.84NVRR90 pKa = 11.84AEE92 pKa = 3.88YY93 pKa = 8.71EE94 pKa = 4.07QEE96 pKa = 3.75RR97 pKa = 11.84KK98 pKa = 9.61RR99 pKa = 11.84RR100 pKa = 11.84LRR102 pKa = 11.84PWLYY106 pKa = 10.66DD107 pKa = 3.11GTPQKK112 pKa = 10.53HH113 pKa = 6.08SRR115 pKa = 11.84DD116 pKa = 4.06PYY118 pKa = 10.08WFDD121 pKa = 3.01VTYY124 pKa = 11.33NQMFKK129 pKa = 10.23KK130 pKa = 9.86WSEE133 pKa = 3.76AA134 pKa = 3.32

Molecular weight:
16.69 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

49

0

49

11938

54

2066

243.6

27.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.964 ± 0.736

0.494 ± 0.134

6.257 ± 0.293

7.48 ± 0.466

3.753 ± 0.255

6.324 ± 0.598

1.684 ± 0.142

7.145 ± 0.417

9.759 ± 0.705

8.335 ± 0.32

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.538 ± 0.154

6.567 ± 0.306

2.781 ± 0.324

3.661 ± 0.175

3.996 ± 0.234

6.492 ± 0.423

5.663 ± 0.303

5.688 ± 0.276

1.164 ± 0.138

4.255 ± 0.469

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski