Staphylococcus virus phi12
Average proteome isoelectric point is 6.16
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q8SDR4|Q8SDR4_9CAUD Uncharacterized protein OS=Staphylococcus virus phi12 OX=186152 PE=4 SV=1
MM1 pKa = 7.06 ATQRR5 pKa = 11.84 QVEE8 pKa = 4.5 YY9 pKa = 11.4 VMSLQEE15 pKa = 3.92 QLEE18 pKa = 4.35 LEE20 pKa = 4.45 DD21 pKa = 3.95 CEE23 pKa = 5.56 KK24 pKa = 10.67 YY25 pKa = 9.42 TDD27 pKa = 3.83 EE28 pKa = 4.01 QVKK31 pKa = 10.4 AMSHH35 pKa = 6.03 KK36 pKa = 10.06 EE37 pKa = 3.72 VSNVIEE43 pKa = 4.41 NYY45 pKa = 9.42 KK46 pKa = 10.77 ASISNEE52 pKa = 3.8 EE53 pKa = 4.63 LYY55 pKa = 10.85 DD56 pKa = 3.48 EE57 pKa = 4.93 CMSFGLPNCC66 pKa = 4.51
Molecular weight: 7.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.045
IPC2_protein 4.342
IPC_protein 4.177
Toseland 4.037
ProMoST 4.266
Dawson 4.101
Bjellqvist 4.253
Wikipedia 3.948
Rodwell 4.012
Grimsley 3.948
Solomon 4.088
Lehninger 4.037
Nozaki 4.228
DTASelect 4.279
Thurlkill 4.037
EMBOSS 3.973
Sillero 4.279
Patrickios 0.846
IPC_peptide 4.101
IPC2_peptide 4.266
IPC2.peptide.svr19 4.189
Protein with the highest isoelectric point:
>tr|Q8SDR6|Q8SDR6_9CAUD ETA orf 26-like protein OS=Staphylococcus virus phi12 OX=186152 PE=4 SV=1
MM1 pKa = 6.5 MARR4 pKa = 11.84 RR5 pKa = 11.84 KK6 pKa = 9.8 VIRR9 pKa = 11.84 VRR11 pKa = 11.84 IKK13 pKa = 10.5 GKK15 pKa = 10.6 LMTLRR20 pKa = 11.84 EE21 pKa = 4.12 VSEE24 pKa = 4.8 KK25 pKa = 10.69 YY26 pKa = 10.32 HH27 pKa = 6.64 ISPEE31 pKa = 4.02 LLRR34 pKa = 11.84 YY35 pKa = 9.46 RR36 pKa = 11.84 YY37 pKa = 8.24 KK38 pKa = 10.75 HH39 pKa = 6.21 KK40 pKa = 10.45 MRR42 pKa = 11.84 GDD44 pKa = 3.41 EE45 pKa = 4.19 LLCGRR50 pKa = 11.84 KK51 pKa = 9.42 DD52 pKa = 3.5 SKK54 pKa = 11.27 SKK56 pKa = 11.42 EE57 pKa = 3.71 EE58 pKa = 3.88 VEE60 pKa = 4.3 YY61 pKa = 10.09 MKK63 pKa = 10.92 SQIKK67 pKa = 10.48 DD68 pKa = 3.39 EE69 pKa = 4.37 EE70 pKa = 4.21 KK71 pKa = 10.52 GRR73 pKa = 11.84 EE74 pKa = 4.07 KK75 pKa = 10.34 IRR77 pKa = 11.84 KK78 pKa = 8.34 KK79 pKa = 10.82 AILNRR84 pKa = 11.84 YY85 pKa = 5.97 QRR87 pKa = 11.84 NVRR90 pKa = 11.84 AEE92 pKa = 3.88 YY93 pKa = 8.71 EE94 pKa = 4.07 QEE96 pKa = 3.75 RR97 pKa = 11.84 KK98 pKa = 9.61 RR99 pKa = 11.84 RR100 pKa = 11.84 LRR102 pKa = 11.84 PWLYY106 pKa = 10.66 DD107 pKa = 3.11 GTPQKK112 pKa = 10.53 HH113 pKa = 6.08 SRR115 pKa = 11.84 DD116 pKa = 4.06 PYY118 pKa = 10.08 WFDD121 pKa = 3.01 VTYY124 pKa = 11.33 NQMFKK129 pKa = 10.23 KK130 pKa = 9.86 WSEE133 pKa = 3.76 AA134 pKa = 3.32
Molecular weight: 16.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.209
IPC2_protein 9.545
IPC_protein 9.692
Toseland 10.321
ProMoST 9.94
Dawson 10.467
Bjellqvist 10.116
Wikipedia 10.628
Rodwell 10.935
Grimsley 10.526
Solomon 10.496
Lehninger 10.467
Nozaki 10.292
DTASelect 10.116
Thurlkill 10.335
EMBOSS 10.701
Sillero 10.379
Patrickios 10.613
IPC_peptide 10.496
IPC2_peptide 8.697
IPC2.peptide.svr19 8.661
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
49
0
49
11938
54
2066
243.6
27.71
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.964 ± 0.736
0.494 ± 0.134
6.257 ± 0.293
7.48 ± 0.466
3.753 ± 0.255
6.324 ± 0.598
1.684 ± 0.142
7.145 ± 0.417
9.759 ± 0.705
8.335 ± 0.32
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.538 ± 0.154
6.567 ± 0.306
2.781 ± 0.324
3.661 ± 0.175
3.996 ± 0.234
6.492 ± 0.423
5.663 ± 0.303
5.688 ± 0.276
1.164 ± 0.138
4.255 ± 0.469
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here