Tomato golden mosaic virus (strain Yellow vein) (TGMV)
Average proteome isoelectric point is 8.55
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|P03567|REP_TGMVY Replication-associated protein OS=Tomato golden mosaic virus (strain Yellow vein) OX=223341 GN=AC1 PE=1 SV=1
MM1 pKa = 8.21 DD2 pKa = 4.49 SQLACPPNVFNYY14 pKa = 9.87 IEE16 pKa = 4.45 SNRR19 pKa = 11.84 DD20 pKa = 3.15 EE21 pKa = 4.43 YY22 pKa = 11.33 QLSHH26 pKa = 7.49 DD27 pKa = 3.8 LTEE30 pKa = 5.64 IILQFPSTASQLSARR45 pKa = 11.84 LSRR48 pKa = 11.84 SCMKK52 pKa = 9.84 IDD54 pKa = 3.15 HH55 pKa = 6.44 CVIEE59 pKa = 4.93 FRR61 pKa = 11.84 QQVPINATGSVVVEE75 pKa = 3.79 IHH77 pKa = 7.05 DD78 pKa = 4.74 KK79 pKa = 11.31 RR80 pKa = 11.84 MTDD83 pKa = 3.41 NEE85 pKa = 4.36 SLQASWTFPVRR96 pKa = 11.84 CNIDD100 pKa = 2.94 LHH102 pKa = 6.2 YY103 pKa = 10.34 FSSSFFSLKK112 pKa = 10.83 DD113 pKa = 3.74 PIPWKK118 pKa = 10.43 LYY120 pKa = 10.92 YY121 pKa = 9.85 KK122 pKa = 10.3 VCDD125 pKa = 4.04 SNVHH129 pKa = 5.15 QRR131 pKa = 11.84 THH133 pKa = 5.39 FAKK136 pKa = 10.85 FKK138 pKa = 10.96 GKK140 pKa = 10.52 LKK142 pKa = 10.82 LSTAKK147 pKa = 10.37 HH148 pKa = 5.69 SVDD151 pKa = 2.95 IPFRR155 pKa = 11.84 APTVKK160 pKa = 10.11 ILSKK164 pKa = 10.79 QFTDD168 pKa = 3.13 KK169 pKa = 11.25 DD170 pKa = 3.51 VDD172 pKa = 4.26 FSHH175 pKa = 7.01 VGYY178 pKa = 10.68 GKK180 pKa = 9.29 WDD182 pKa = 3.22 RR183 pKa = 11.84 KK184 pKa = 8.99 MIRR187 pKa = 11.84 SASTSTIGLTGPIEE201 pKa = 4.01 LRR203 pKa = 11.84 PGEE206 pKa = 4.29 SWASRR211 pKa = 11.84 STIGPSPSYY220 pKa = 10.69 AGSDD224 pKa = 3.31 QGDD227 pKa = 3.21 AMHH230 pKa = 7.5 PYY232 pKa = 9.94 KK233 pKa = 10.61 HH234 pKa = 6.44 LNRR237 pKa = 11.84 LGTTILDD244 pKa = 4.09 PGEE247 pKa = 4.09 SASIIGAEE255 pKa = 4.03 RR256 pKa = 11.84 TQSNITMSMVQLNEE270 pKa = 3.69 IVRR273 pKa = 11.84 ATVQEE278 pKa = 4.67 CINTNCTPSQPKK290 pKa = 8.53 TLQQ293 pKa = 3.14
Molecular weight: 32.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.141
IPC2_protein 7.454
IPC_protein 7.38
Toseland 7.102
ProMoST 7.863
Dawson 8.053
Bjellqvist 8.419
Wikipedia 7.966
Rodwell 8.068
Grimsley 7.176
Solomon 8.141
Lehninger 8.17
Nozaki 8.682
DTASelect 8.17
Thurlkill 8.229
EMBOSS 8.287
Sillero 8.551
Patrickios 4.38
IPC_peptide 8.141
IPC2_peptide 7.527
IPC2.peptide.svr19 7.473
Protein with the highest isoelectric point:
>sp|P03562|TRAP_TGMVY Transcriptional activator protein OS=Tomato golden mosaic virus (strain Yellow vein) OX=223341 GN=AC2 PE=1 SV=1
MM1 pKa = 7.98 PKK3 pKa = 9.65 RR4 pKa = 11.84 DD5 pKa = 3.82 APWRR9 pKa = 11.84 LMAGTSKK16 pKa = 10.61 VSRR19 pKa = 11.84 SANYY23 pKa = 9.82 SPRR26 pKa = 11.84 GSLPKK31 pKa = 9.79 RR32 pKa = 11.84 DD33 pKa = 3.11 AWVNRR38 pKa = 11.84 PMYY41 pKa = 9.63 RR42 pKa = 11.84 KK43 pKa = 8.92 PRR45 pKa = 11.84 IYY47 pKa = 10.27 RR48 pKa = 11.84 SLRR51 pKa = 11.84 GPDD54 pKa = 3.32 VPKK57 pKa = 10.83 GCEE60 pKa = 4.39 GPCKK64 pKa = 9.35 VQSYY68 pKa = 6.94 EE69 pKa = 3.78 QRR71 pKa = 11.84 HH72 pKa = 6.34 DD73 pKa = 3.04 ISLVGKK79 pKa = 7.53 VMCISDD85 pKa = 3.56 VTRR88 pKa = 11.84 GNGITHH94 pKa = 6.78 RR95 pKa = 11.84 VGKK98 pKa = 9.44 RR99 pKa = 11.84 FCVKK103 pKa = 9.92 SVYY106 pKa = 10.16 ILGKK110 pKa = 9.37 IWMDD114 pKa = 3.39 EE115 pKa = 4.0 NIKK118 pKa = 10.61 LKK120 pKa = 10.73 NHH122 pKa = 5.95 TNSVMFWLVRR132 pKa = 11.84 DD133 pKa = 3.7 RR134 pKa = 11.84 RR135 pKa = 11.84 PYY137 pKa = 8.05 GTPMDD142 pKa = 4.6 FGQVFNMFDD151 pKa = 3.99 NEE153 pKa = 4.11 PSTATVKK160 pKa = 10.85 NDD162 pKa = 2.82 LRR164 pKa = 11.84 DD165 pKa = 3.5 RR166 pKa = 11.84 FQVIHH171 pKa = 6.91 RR172 pKa = 11.84 FHH174 pKa = 6.98 AKK176 pKa = 8.01 VTGGQYY182 pKa = 11.1 ASNEE186 pKa = 3.84 QALVRR191 pKa = 11.84 RR192 pKa = 11.84 FWKK195 pKa = 10.33 VNNNVVYY202 pKa = 10.41 NHH204 pKa = 5.87 QEE206 pKa = 3.4 AGKK209 pKa = 10.41 YY210 pKa = 8.39 EE211 pKa = 4.09 NHH213 pKa = 6.52 TEE215 pKa = 3.99 NALLLYY221 pKa = 7.29 MACTHH226 pKa = 7.07 ASNPVYY232 pKa = 9.86 ATLKK236 pKa = 9.47 IRR238 pKa = 11.84 IYY240 pKa = 10.59 FYY242 pKa = 11.38 DD243 pKa = 4.2 SITNN247 pKa = 3.6
Molecular weight: 28.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.255
IPC2_protein 9.472
IPC_protein 9.619
Toseland 10.014
ProMoST 9.765
Dawson 10.248
Bjellqvist 9.955
Wikipedia 10.438
Rodwell 10.57
Grimsley 10.335
Solomon 10.277
Lehninger 10.233
Nozaki 10.058
DTASelect 9.94
Thurlkill 10.087
EMBOSS 10.423
Sillero 10.16
Patrickios 9.911
IPC_peptide 10.262
IPC2_peptide 8.829
IPC2.peptide.svr19 8.406
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
1494
85
352
213.4
24.52
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.685 ± 0.204
1.941 ± 0.199
5.02 ± 0.44
4.418 ± 0.559
4.685 ± 0.533
5.154 ± 0.444
3.548 ± 0.14
6.158 ± 0.546
6.024 ± 0.535
7.028 ± 0.665
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.075 ± 0.468
5.89 ± 0.5
5.02 ± 0.525
4.485 ± 0.725
7.296 ± 0.977
9.371 ± 0.955
5.957 ± 0.829
5.756 ± 0.887
1.539 ± 0.167
3.949 ± 0.568
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here