Proteobacteria bacterium CAG:139

Taxonomy: cellular organisms; Bacteria; Proteobacteria; environmental samples

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1880 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6A8M1|R6A8M1_9PROT DNA mismatch repair protein MutS OS=Proteobacteria bacterium CAG:139 OX=1262986 GN=mutS PE=3 SV=1
MM1 pKa = 7.78PGFEE5 pKa = 4.4GTFLGDD11 pKa = 3.32SSKK14 pKa = 10.93LHH16 pKa = 6.8PDD18 pKa = 2.76TRR20 pKa = 11.84LEE22 pKa = 4.25CKK24 pKa = 9.26VCHH27 pKa = 6.35YY28 pKa = 10.48IYY30 pKa = 10.58DD31 pKa = 3.96PSKK34 pKa = 11.37GDD36 pKa = 3.01VDD38 pKa = 4.16QNIEE42 pKa = 3.95PGTPFSEE49 pKa = 4.61LPEE52 pKa = 4.03YY53 pKa = 9.54WNCPVCGCEE62 pKa = 3.62RR63 pKa = 11.84DD64 pKa = 3.44LFMVVPEE71 pKa = 5.86DD72 pKa = 3.55YY73 pKa = 10.84DD74 pKa = 3.5QNQRR78 pKa = 11.84SDD80 pKa = 3.56DD81 pKa = 4.11SKK83 pKa = 11.41SSKK86 pKa = 10.7

Molecular weight: 9.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6AU25|R6AU25_9PROT 50S ribosomal protein L7/L12 OS=Proteobacteria bacterium CAG:139 OX=1262986 GN=rplL PE=3 SV=1
MM1 pKa = 6.85ATKK4 pKa = 9.51RR5 pKa = 11.84TYY7 pKa = 10.39QPSKK11 pKa = 8.92VKK13 pKa = 10.35RR14 pKa = 11.84ARR16 pKa = 11.84THH18 pKa = 5.71GFLVRR23 pKa = 11.84SRR25 pKa = 11.84TRR27 pKa = 11.84GGRR30 pKa = 11.84KK31 pKa = 8.83VLAARR36 pKa = 11.84RR37 pKa = 11.84RR38 pKa = 11.84KK39 pKa = 9.13GRR41 pKa = 11.84HH42 pKa = 3.97VLALL46 pKa = 3.77

Molecular weight: 5.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1880

0

1880

577478

29

1856

307.2

33.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.307 ± 0.06

1.272 ± 0.028

5.17 ± 0.046

6.563 ± 0.065

4.362 ± 0.045

7.442 ± 0.06

1.823 ± 0.027

6.165 ± 0.044

6.344 ± 0.053

9.853 ± 0.068

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.604 ± 0.03

3.797 ± 0.035

4.526 ± 0.034

3.32 ± 0.033

5.1 ± 0.05

6.055 ± 0.048

5.117 ± 0.03

7.268 ± 0.048

1.171 ± 0.025

2.739 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski