Gilliamella apicola SCGC AB-598-I20
Average proteome isoelectric point is 6.58
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2312 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A080KEC7|A0A080KEC7_9GAMM Amino acid transporter OS=Gilliamella apicola SCGC AB-598-I20 OX=1445514 GN=GASC598I20_007610 PE=4 SV=1
MM1 pKa = 7.84 LDD3 pKa = 3.54 YY4 pKa = 11.13 SVGSSIEE11 pKa = 3.84 SDD13 pKa = 3.54 SFNAEE18 pKa = 3.93 LLYY21 pKa = 11.14 DD22 pKa = 3.88 GDD24 pKa = 3.93 IWGEE28 pKa = 3.97 LCLSEE33 pKa = 5.78 DD34 pKa = 3.52 KK35 pKa = 11.22 QNLEE39 pKa = 4.02 FVIYY43 pKa = 9.82 PSDD46 pKa = 3.66 PQRR49 pKa = 11.84 VLTFNYY55 pKa = 9.88 DD56 pKa = 2.9 EE57 pKa = 4.58 LMAFIEE63 pKa = 4.23 LAKK66 pKa = 10.87 NHH68 pKa = 6.72 LLQLDD73 pKa = 4.32 PLVKK77 pKa = 10.53 DD78 pKa = 3.68
Molecular weight: 8.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.753
IPC2_protein 3.821
IPC_protein 3.745
Toseland 3.554
ProMoST 3.846
Dawson 3.745
Bjellqvist 3.961
Wikipedia 3.694
Rodwell 3.579
Grimsley 3.465
Solomon 3.719
Lehninger 3.681
Nozaki 3.872
DTASelect 4.075
Thurlkill 3.617
EMBOSS 3.694
Sillero 3.872
Patrickios 1.875
IPC_peptide 3.719
IPC2_peptide 3.846
IPC2.peptide.svr19 3.829
Protein with the highest isoelectric point:
>tr|A0A080KHM1|A0A080KHM1_9GAMM Protein-export protein SecB OS=Gilliamella apicola SCGC AB-598-I20 OX=1445514 GN=secB PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.68 RR12 pKa = 11.84 NRR14 pKa = 11.84 THH16 pKa = 7.28 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.4 NGRR28 pKa = 11.84 QVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.44 GRR39 pKa = 11.84 VRR41 pKa = 11.84 LTVASS46 pKa = 3.91
Molecular weight: 5.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.647
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 12.998
DTASelect 12.998
Thurlkill 12.998
EMBOSS 13.51
Sillero 12.998
Patrickios 12.369
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.185
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2312
0
2312
617385
20
2677
267.0
29.95
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.43 ± 0.051
1.121 ± 0.02
5.36 ± 0.044
5.561 ± 0.055
4.401 ± 0.042
6.241 ± 0.06
2.016 ± 0.026
8.438 ± 0.051
6.538 ± 0.044
10.34 ± 0.061
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.407 ± 0.025
5.537 ± 0.05
3.625 ± 0.03
4.595 ± 0.042
3.814 ± 0.04
6.371 ± 0.044
5.285 ± 0.034
6.158 ± 0.042
1.124 ± 0.02
3.637 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here