Salmonella phage Det7
Average proteome isoelectric point is 6.26
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 210 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0C5PQT4|A0A0C5PQT4_9CAUD Uncharacterized protein OS=Salmonella phage Det7 OX=454798 GN=192 PE=4 SV=1
MM1 pKa = 7.54 KK2 pKa = 10.25 LKK4 pKa = 10.61 KK5 pKa = 10.1 DD6 pKa = 3.72 VLFHH10 pKa = 7.73 KK11 pKa = 10.2 IMQVFAVTTMAFSLSACATLLDD33 pKa = 4.79 SVDD36 pKa = 3.9 LDD38 pKa = 3.99 APTFTNQQAVNKK50 pKa = 9.85 MEE52 pKa = 4.2 DD53 pKa = 3.51 TIKK56 pKa = 11.04 AHH58 pKa = 6.87 AALDD62 pKa = 3.77 NTTPGPLQTVCNYY75 pKa = 10.58 DD76 pKa = 3.97 DD77 pKa = 5.54 SIQEE81 pKa = 4.24 DD82 pKa = 3.93 EE83 pKa = 4.95 TYY85 pKa = 10.8 HH86 pKa = 5.37 CTTYY90 pKa = 10.81 VKK92 pKa = 10.2 EE93 pKa = 4.33 SSVVLYY99 pKa = 10.72 ADD101 pKa = 3.77 CTEE104 pKa = 4.32 EE105 pKa = 3.89 QCTATGYY112 pKa = 10.78 DD113 pKa = 3.34 QVEE116 pKa = 4.46 KK117 pKa = 10.86 SDD119 pKa = 3.61 EE120 pKa = 3.95
Molecular weight: 13.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.96
IPC2_protein 4.24
IPC_protein 4.177
Toseland 3.986
ProMoST 4.279
Dawson 4.151
Bjellqvist 4.355
Wikipedia 4.075
Rodwell 4.012
Grimsley 3.897
Solomon 4.151
Lehninger 4.101
Nozaki 4.266
DTASelect 4.482
Thurlkill 4.024
EMBOSS 4.088
Sillero 4.291
Patrickios 1.074
IPC_peptide 4.151
IPC2_peptide 4.279
IPC2.peptide.svr19 4.203
Protein with the highest isoelectric point:
>tr|A0A0C5PV19|A0A0C5PV19_9CAUD Uncharacterized protein OS=Salmonella phage Det7 OX=454798 GN=53 PE=4 SV=1
MM1 pKa = 7.04 SWKK4 pKa = 10.26 LFKK7 pKa = 10.48 LRR9 pKa = 11.84 KK10 pKa = 8.71 ATDD13 pKa = 3.33 EE14 pKa = 4.22 FLLKK18 pKa = 10.78 ARR20 pKa = 11.84 MEE22 pKa = 4.14 GRR24 pKa = 11.84 LVYY27 pKa = 9.82 TPYY30 pKa = 10.56 PGTTRR35 pKa = 11.84 FCSTLRR41 pKa = 11.84 KK42 pKa = 8.85 QKK44 pKa = 10.47 SVSGPRR50 pKa = 11.84 RR51 pKa = 11.84 LMNQGMLSFNTGDD64 pKa = 4.28 HH65 pKa = 6.48 RR66 pKa = 11.84 PCDD69 pKa = 3.57 GGRR72 pKa = 11.84 III74 pKa = 5.68
Molecular weight: 8.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.336
IPC2_protein 9.78
IPC_protein 10.599
Toseland 10.906
ProMoST 10.613
Dawson 10.965
Bjellqvist 10.687
Wikipedia 11.184
Rodwell 11.213
Grimsley 11.008
Solomon 11.125
Lehninger 11.096
Nozaki 10.891
DTASelect 10.672
Thurlkill 10.891
EMBOSS 11.301
Sillero 10.906
Patrickios 10.979
IPC_peptide 11.125
IPC2_peptide 9.78
IPC2.peptide.svr19 8.547
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
210
0
210
48777
38
1640
232.3
26.22
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.601 ± 0.142
1.169 ± 0.075
6.314 ± 0.098
6.612 ± 0.2
4.32 ± 0.103
6.733 ± 0.2
1.921 ± 0.113
6.337 ± 0.104
6.466 ± 0.168
8.041 ± 0.132
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.958 ± 0.097
5.24 ± 0.135
3.893 ± 0.108
3.756 ± 0.122
4.961 ± 0.128
6.505 ± 0.185
5.874 ± 0.204
7.014 ± 0.173
1.408 ± 0.072
3.877 ± 0.114
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here