Circoviridae 8 LDMD-2013

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; environmental samples

Average proteome isoelectric point is 8.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S5TND2|S5TND2_9CIRC Uncharacterized protein OS=Circoviridae 8 LDMD-2013 OX=1379712 PE=4 SV=1
MM1 pKa = 8.01IPICHH6 pKa = 6.92KK7 pKa = 10.52GGCGSFWLTKK17 pKa = 10.32KK18 pKa = 10.49KK19 pKa = 10.73LEE21 pKa = 3.93RR22 pKa = 11.84TEE24 pKa = 4.54RR25 pKa = 11.84CICRR29 pKa = 11.84VMCTLKK35 pKa = 8.4MTKK38 pKa = 9.16TFSTVRR44 pKa = 11.84NLCPQAHH51 pKa = 6.06WEE53 pKa = 4.0PRR55 pKa = 11.84KK56 pKa = 10.19GSHH59 pKa = 5.0QQAVDD64 pKa = 3.51YY65 pKa = 7.47CTKK68 pKa = 10.71EE69 pKa = 3.66EE70 pKa = 4.0TRR72 pKa = 11.84RR73 pKa = 11.84PGCDD77 pKa = 2.66PVVVGDD83 pKa = 4.64PPSQGKK89 pKa = 7.91RR90 pKa = 11.84TDD92 pKa = 3.08IMDD95 pKa = 3.45VKK97 pKa = 11.09SFIDD101 pKa = 3.95GGCSRR106 pKa = 11.84EE107 pKa = 4.18EE108 pKa = 3.61LWSEE112 pKa = 4.01HH113 pKa = 5.31FSAMSKK119 pKa = 9.39MYY121 pKa = 10.48KK122 pKa = 10.42AMYY125 pKa = 8.71EE126 pKa = 4.0YY127 pKa = 11.12SFVRR131 pKa = 11.84SGEE134 pKa = 4.38RR135 pKa = 11.84NWKK138 pKa = 7.92TRR140 pKa = 11.84VTVIVGPPGTGKK152 pKa = 11.22SMFCNNYY159 pKa = 9.54HH160 pKa = 5.98GQSYY164 pKa = 8.27WKK166 pKa = 10.4NNSLFFDD173 pKa = 5.37GYY175 pKa = 10.23HH176 pKa = 5.44GQDD179 pKa = 3.96VIVMDD184 pKa = 4.83DD185 pKa = 4.57FYY187 pKa = 11.8GQLPWSTLLKK197 pKa = 10.63LADD200 pKa = 4.4RR201 pKa = 11.84YY202 pKa = 11.18KK203 pKa = 11.07LILDD207 pKa = 3.78IKK209 pKa = 9.45GSSVNMLAKK218 pKa = 10.3EE219 pKa = 4.03LFITSNADD227 pKa = 2.86IEE229 pKa = 4.35EE230 pKa = 4.51WYY232 pKa = 10.29SYY234 pKa = 10.62KK235 pKa = 10.54PGMDD239 pKa = 2.51IRR241 pKa = 11.84ALEE244 pKa = 3.92RR245 pKa = 11.84RR246 pKa = 11.84IDD248 pKa = 3.4HH249 pKa = 5.81YY250 pKa = 12.0VNVDD254 pKa = 3.25EE255 pKa = 6.14SGTITNRR262 pKa = 11.84SSYY265 pKa = 9.85EE266 pKa = 3.46PHH268 pKa = 7.49KK269 pKa = 10.86ILTNGKK275 pKa = 9.48KK276 pKa = 10.21LVDD279 pKa = 3.61MANLIKK285 pKa = 10.98NFVFSYY291 pKa = 10.33KK292 pKa = 10.05PSEE295 pKa = 4.68DD296 pKa = 3.2GSGFSTNVTIDD307 pKa = 3.03EE308 pKa = 4.43YY309 pKa = 10.79EE310 pKa = 3.93IRR312 pKa = 11.84RR313 pKa = 11.84HH314 pKa = 4.76LTVNPEE320 pKa = 3.91VLSAPEE326 pKa = 3.94FLEE329 pKa = 5.34LSDD332 pKa = 4.94KK333 pKa = 10.99LSHH336 pKa = 6.23TLNEE340 pKa = 4.09LEE342 pKa = 4.26YY343 pKa = 10.69QVAEE347 pKa = 4.35LRR349 pKa = 11.84RR350 pKa = 11.84KK351 pKa = 8.58VANEE355 pKa = 3.77LVDD358 pKa = 3.81VPINFQRR365 pKa = 11.84EE366 pKa = 4.18SSSNLSARR374 pKa = 11.84DD375 pKa = 3.23IMEE378 pKa = 3.93VAEE381 pKa = 4.2RR382 pKa = 11.84AVEE385 pKa = 4.11EE386 pKa = 4.49VRR388 pKa = 11.84GHH390 pKa = 6.86PNLEE394 pKa = 4.04LEE396 pKa = 4.86DD397 pKa = 3.61RR398 pKa = 11.84HH399 pKa = 5.77HH400 pKa = 7.16TYY402 pKa = 8.5GTPTPRR408 pKa = 11.84KK409 pKa = 9.26RR410 pKa = 11.84RR411 pKa = 11.84RR412 pKa = 11.84DD413 pKa = 3.52EE414 pKa = 4.27PNISPRR420 pKa = 11.84SRR422 pKa = 11.84RR423 pKa = 11.84PPRR426 pKa = 11.84IGRR429 pKa = 11.84SGGEE433 pKa = 3.87NIIPMRR439 pKa = 11.84DD440 pKa = 3.31VNEE443 pKa = 4.4LSNSSEE449 pKa = 3.85GEE451 pKa = 4.17SFNTDD456 pKa = 2.51EE457 pKa = 5.85DD458 pKa = 3.8EE459 pKa = 6.12AEE461 pKa = 4.19MYY463 pKa = 10.7EE464 pKa = 4.22EE465 pKa = 4.75GLNTQHH471 pKa = 6.7TSEE474 pKa = 4.27TLGTEE479 pKa = 4.34DD480 pKa = 4.04TMEE483 pKa = 4.01

Molecular weight: 55.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S5SYD5|S5SYD5_9CIRC Putative viral capsid OS=Circoviridae 8 LDMD-2013 OX=1379712 PE=4 SV=1
MM1 pKa = 7.85GKK3 pKa = 9.66RR4 pKa = 11.84KK5 pKa = 9.66SRR7 pKa = 11.84SRR9 pKa = 11.84QTSISKK15 pKa = 8.41YY16 pKa = 6.68VRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84RR21 pKa = 11.84TNFSQTPQPPPLYY34 pKa = 10.28HH35 pKa = 6.44RR36 pKa = 11.84SKK38 pKa = 10.22WKK40 pKa = 9.87YY41 pKa = 9.3AGNMWVRR48 pKa = 11.84KK49 pKa = 9.38NKK51 pKa = 10.64GEE53 pKa = 3.95IHH55 pKa = 6.86FDD57 pKa = 3.04RR58 pKa = 11.84YY59 pKa = 10.74GYY61 pKa = 9.28PLPSAVNRR69 pKa = 11.84EE70 pKa = 4.08EE71 pKa = 4.08YY72 pKa = 10.61KK73 pKa = 10.61DD74 pKa = 3.44WKK76 pKa = 10.14APYY79 pKa = 9.71YY80 pKa = 10.13PWDD83 pKa = 4.22EE84 pKa = 3.98KK85 pKa = 10.96WKK87 pKa = 7.17TTSYY91 pKa = 10.94TNAGYY96 pKa = 10.75AWGTPIYY103 pKa = 10.24DD104 pKa = 3.95DD105 pKa = 3.96QRR107 pKa = 11.84LMGHH111 pKa = 5.66KK112 pKa = 9.69VRR114 pKa = 11.84RR115 pKa = 11.84RR116 pKa = 11.84DD117 pKa = 3.32GLYY120 pKa = 10.71EE121 pKa = 4.19EE122 pKa = 5.98LPADD126 pKa = 3.46WVLPPTTRR134 pKa = 11.84PTMTRR139 pKa = 11.84LQNTLFDD146 pKa = 4.15KK147 pKa = 10.52TYY149 pKa = 8.99HH150 pKa = 6.75PYY152 pKa = 10.27RR153 pKa = 11.84YY154 pKa = 9.82GYY156 pKa = 8.53TRR158 pKa = 11.84ALDD161 pKa = 3.11WARR164 pKa = 11.84RR165 pKa = 11.84HH166 pKa = 6.46RR167 pKa = 11.84IAVWIIKK174 pKa = 8.99RR175 pKa = 11.84IWWGFVLKK183 pKa = 10.88NGIYY187 pKa = 8.31PTEE190 pKa = 4.84DD191 pKa = 2.81FLQRR195 pKa = 11.84QVEE198 pKa = 4.2KK199 pKa = 10.55FKK201 pKa = 10.97RR202 pKa = 11.84YY203 pKa = 7.48MQGKK207 pKa = 8.48VSSSEE212 pKa = 3.61ADD214 pKa = 3.14PVVKK218 pKa = 10.57NPPQRR223 pKa = 11.84PIHH226 pKa = 5.96PSQRR230 pKa = 11.84PSKK233 pKa = 9.66IRR235 pKa = 11.84SRR237 pKa = 11.84IKK239 pKa = 10.21ANYY242 pKa = 8.02YY243 pKa = 10.16

Molecular weight: 29.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1228

102

483

245.6

28.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.479 ± 0.878

1.792 ± 0.505

4.479 ± 0.67

5.863 ± 1.567

3.502 ± 0.526

6.026 ± 0.537

3.583 ± 0.512

5.7 ± 0.54

7.248 ± 1.037

7.003 ± 1.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.199 ± 0.501

4.805 ± 0.334

6.27 ± 0.762

3.176 ± 0.487

7.736 ± 1.061

8.225 ± 1.01

5.863 ± 0.353

5.375 ± 0.453

1.954 ± 0.632

4.642 ± 0.914
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski