Paenibacillus sp. FSL H8-0548

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus; unclassified Paenibacillus

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5977 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1R1CN20|A0A1R1CN20_9BACL Anti-sigma B factor RsbW OS=Paenibacillus sp. FSL H8-0548 OX=1920422 GN=BK133_24485 PE=4 SV=1
MM1 pKa = 7.54AANSALLFEE10 pKa = 5.47FNDD13 pKa = 3.91DD14 pKa = 3.1QSAALACDD22 pKa = 3.59TLRR25 pKa = 11.84EE26 pKa = 4.1LGYY29 pKa = 9.23EE30 pKa = 4.27TVLHH34 pKa = 6.45EE35 pKa = 4.47GSRR38 pKa = 11.84MHH40 pKa = 5.9VHH42 pKa = 7.0LEE44 pKa = 4.08GSDD47 pKa = 3.35LTSALEE53 pKa = 3.99IAQSHH58 pKa = 6.5GGQLAQQALINADD71 pKa = 3.38AVTDD75 pKa = 3.66TAYY78 pKa = 11.2SLDD81 pKa = 4.11TITIPAHH88 pKa = 6.18LVNEE92 pKa = 4.54DD93 pKa = 3.78LEE95 pKa = 4.48SLYY98 pKa = 11.23GDD100 pKa = 3.81GEE102 pKa = 4.28LPEE105 pKa = 5.09GVSNYY110 pKa = 10.91DD111 pKa = 2.79NDD113 pKa = 3.85ADD115 pKa = 4.14YY116 pKa = 11.24EE117 pKa = 4.84GEE119 pKa = 4.08FLPDD123 pKa = 3.94PGTYY127 pKa = 10.56DD128 pKa = 3.2HH129 pKa = 7.31FSGDD133 pKa = 3.05VRR135 pKa = 11.84AA136 pKa = 4.74

Molecular weight: 14.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1R1CL04|A0A1R1CL04_9BACL Uncharacterized protein OS=Paenibacillus sp. FSL H8-0548 OX=1920422 GN=BK133_25395 PE=4 SV=1
MM1 pKa = 7.82RR2 pKa = 11.84PTFRR6 pKa = 11.84PNVSKK11 pKa = 10.64RR12 pKa = 11.84AKK14 pKa = 8.72VHH16 pKa = 5.43GFRR19 pKa = 11.84KK20 pKa = 10.04RR21 pKa = 11.84MSTKK25 pKa = 10.06NGRR28 pKa = 11.84KK29 pKa = 8.88ILATRR34 pKa = 11.84RR35 pKa = 11.84QRR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.82VLSAA44 pKa = 4.11

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5977

0

5977

2063183

17

3327

345.2

38.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.278 ± 0.042

0.688 ± 0.011

5.13 ± 0.024

6.703 ± 0.033

4.243 ± 0.025

7.115 ± 0.032

2.026 ± 0.018

7.152 ± 0.032

5.431 ± 0.026

10.033 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.788 ± 0.017

4.152 ± 0.028

3.797 ± 0.02

3.92 ± 0.02

4.586 ± 0.03

6.664 ± 0.027

5.502 ± 0.031

6.868 ± 0.025

1.344 ± 0.013

3.581 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski