Xenophilus sp. AP218F

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Comamonadaceae; Xenophilus; unclassified Xenophilus

Average proteome isoelectric point is 6.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3588 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A254VN13|A0A254VN13_9BURK Uncharacterized protein OS=Xenophilus sp. AP218F OX=2014876 GN=CEK28_02895 PE=4 SV=1
MM1 pKa = 7.55TDD3 pKa = 2.83VATEE7 pKa = 4.24MPCPINFTDD16 pKa = 3.82SACAKK21 pKa = 9.61VQDD24 pKa = 5.36LIAEE28 pKa = 4.34EE29 pKa = 4.65GNPDD33 pKa = 2.99LKK35 pKa = 11.14LRR37 pKa = 11.84VFVTGGGCSGFQYY50 pKa = 10.94GFTFDD55 pKa = 4.65EE56 pKa = 4.88IANEE60 pKa = 4.13DD61 pKa = 3.33DD62 pKa = 3.66TAIEE66 pKa = 4.11RR67 pKa = 11.84QGVTFLVDD75 pKa = 3.46PMSYY79 pKa = 10.19QYY81 pKa = 11.57LVGAEE86 pKa = 3.7IDD88 pKa = 3.88YY89 pKa = 11.15QEE91 pKa = 4.55SLEE94 pKa = 4.16GSQFVIRR101 pKa = 11.84NPNATTTCGCGSSFSVV117 pKa = 3.54

Molecular weight:
12.66 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A254VL04|A0A254VL04_9BURK Phosphatase NudJ OS=Xenophilus sp. AP218F OX=2014876 GN=nudJ PE=3 SV=1
MM1 pKa = 7.75LFRR4 pKa = 11.84KK5 pKa = 10.21RR6 pKa = 11.84SWQAFRR12 pKa = 11.84LRR14 pKa = 11.84PRR16 pKa = 11.84EE17 pKa = 3.96SPRR20 pKa = 11.84RR21 pKa = 11.84AVRR24 pKa = 11.84PPTNPRR30 pKa = 11.84CRR32 pKa = 11.84EE33 pKa = 3.81LPSRR37 pKa = 11.84LRR39 pKa = 11.84RR40 pKa = 11.84SRR42 pKa = 11.84LRR44 pKa = 11.84RR45 pKa = 11.84SRR47 pKa = 11.84LRR49 pKa = 11.84RR50 pKa = 11.84SRR52 pKa = 11.84LRR54 pKa = 11.84RR55 pKa = 11.84SRR57 pKa = 11.84LRR59 pKa = 11.84RR60 pKa = 11.84SRR62 pKa = 11.84LRR64 pKa = 11.84RR65 pKa = 11.84SRR67 pKa = 11.84LRR69 pKa = 11.84RR70 pKa = 11.84SRR72 pKa = 11.84LRR74 pKa = 11.84RR75 pKa = 11.84SRR77 pKa = 11.84LRR79 pKa = 11.84RR80 pKa = 11.84SRR82 pKa = 11.84LRR84 pKa = 11.84QSPLRR89 pKa = 11.84QSRR92 pKa = 11.84LRR94 pKa = 11.84QSRR97 pKa = 11.84LRR99 pKa = 11.84QSQRR103 pKa = 11.84HH104 pKa = 4.82PLRR107 pKa = 11.84RR108 pKa = 11.84RR109 pKa = 11.84PRR111 pKa = 11.84QKK113 pKa = 10.51ARR115 pKa = 11.84IRR117 pKa = 11.84RR118 pKa = 11.84RR119 pKa = 3.23

Molecular weight:
15.39 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3588

0

3588

1168738

28

4570

325.7

35.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.7 ± 0.059

1.011 ± 0.016

5.349 ± 0.031

5.498 ± 0.038

3.498 ± 0.028

8.075 ± 0.041

2.084 ± 0.018

4.513 ± 0.029

3.641 ± 0.043

11.59 ± 0.064

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.441 ± 0.019

2.879 ± 0.027

4.949 ± 0.032

4.429 ± 0.027

6.819 ± 0.047

5.544 ± 0.037

4.284 ± 0.035

6.754 ± 0.038

1.454 ± 0.021

2.487 ± 0.022

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski