Xenophilus sp. AP218F 
Average proteome isoelectric point is 6.81 
Get precalculated fractions of proteins 
 
  
    Acidic  
     
   
 
  
    pI < 6.8  
     
   
 
  
    6.8-7.4  
     
   
 
  
    pI > 7.4  
     
   
 
  
    Basic  
     
   
    
 
  
    All  
     
   
 
 
Note: above files contain also dissociation constants (pKa) 
 
Virtual 2D-PAGE plot for 3588 proteins (isoelectric point calculated using IPC2_protein) 
 
Get csv file with sequences according to given criteria: 
* You can choose from 21 different methods for calculating isoelectric point 
 
 Summary statistics related to proteome-wise predictions 
 
Protein with the lowest isoelectric point: 
>tr|A0A254VN13|A0A254VN13_9BURK Uncharacterized protein OS=Xenophilus sp. AP218F OX=2014876 GN=CEK28_02895 PE=4 SV=1 
MM1 pKa = 7.55  TDD3 pKa = 2.83  VATEE7 pKa = 4.24  MPCPINFTDD16 pKa = 3.82  SACAKK21 pKa = 9.61  VQDD24 pKa = 5.36  LIAEE28 pKa = 4.34  EE29 pKa = 4.65  GNPDD33 pKa = 2.99  LKK35 pKa = 11.14  LRR37 pKa = 11.84  VFVTGGGCSGFQYY50 pKa = 10.94  GFTFDD55 pKa = 4.65  EE56 pKa = 4.88  IANEE60 pKa = 4.13  DD61 pKa = 3.33  DD62 pKa = 3.66  TAIEE66 pKa = 4.11  RR67 pKa = 11.84  QGVTFLVDD75 pKa = 3.46  PMSYY79 pKa = 10.19  QYY81 pKa = 11.57  LVGAEE86 pKa = 3.7  IDD88 pKa = 3.88  YY89 pKa = 11.15  QEE91 pKa = 4.55  SLEE94 pKa = 4.16  GSQFVIRR101 pKa = 11.84  NPNATTTCGCGSSFSVV117 pKa = 3.54   
 Molecular weight: 12.66 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  3.82 
IPC2_protein 3.77 
IPC_protein 3.706 
Toseland    3.516 
ProMoST     3.872 
Dawson      3.694 
Bjellqvist  3.859 
Wikipedia   3.63 
Rodwell     3.541 
Grimsley    3.427 
Solomon     3.668 
Lehninger   3.63 
Nozaki      3.821 
DTASelect   3.999 
Thurlkill   3.567 
EMBOSS      3.643 
Sillero     3.821 
Patrickios  0.299 
IPC_peptide 3.668 
IPC2_peptide  3.795 
IPC2.peptide.svr19  3.759 
 Protein with the highest isoelectric point: 
>tr|A0A254VL04|A0A254VL04_9BURK Phosphatase NudJ OS=Xenophilus sp. AP218F OX=2014876 GN=nudJ PE=3 SV=1 
MM1 pKa = 7.75  LFRR4 pKa = 11.84  KK5 pKa = 10.21  RR6 pKa = 11.84  SWQAFRR12 pKa = 11.84  LRR14 pKa = 11.84  PRR16 pKa = 11.84  EE17 pKa = 3.96  SPRR20 pKa = 11.84  RR21 pKa = 11.84  AVRR24 pKa = 11.84  PPTNPRR30 pKa = 11.84  CRR32 pKa = 11.84  EE33 pKa = 3.81  LPSRR37 pKa = 11.84  LRR39 pKa = 11.84  RR40 pKa = 11.84  SRR42 pKa = 11.84  LRR44 pKa = 11.84  RR45 pKa = 11.84  SRR47 pKa = 11.84  LRR49 pKa = 11.84  RR50 pKa = 11.84  SRR52 pKa = 11.84  LRR54 pKa = 11.84  RR55 pKa = 11.84  SRR57 pKa = 11.84  LRR59 pKa = 11.84  RR60 pKa = 11.84  SRR62 pKa = 11.84  LRR64 pKa = 11.84  RR65 pKa = 11.84  SRR67 pKa = 11.84  LRR69 pKa = 11.84  RR70 pKa = 11.84  SRR72 pKa = 11.84  LRR74 pKa = 11.84  RR75 pKa = 11.84  SRR77 pKa = 11.84  LRR79 pKa = 11.84  RR80 pKa = 11.84  SRR82 pKa = 11.84  LRR84 pKa = 11.84  QSPLRR89 pKa = 11.84  QSRR92 pKa = 11.84  LRR94 pKa = 11.84  QSRR97 pKa = 11.84  LRR99 pKa = 11.84  QSQRR103 pKa = 11.84  HH104 pKa = 4.82  PLRR107 pKa = 11.84  RR108 pKa = 11.84  RR109 pKa = 11.84  PRR111 pKa = 11.84  QKK113 pKa = 10.51  ARR115 pKa = 11.84  IRR117 pKa = 11.84  RR118 pKa = 11.84  RR119 pKa = 3.23   
 Molecular weight: 15.39 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  9.527 
IPC2_protein 11.316 
IPC_protein 12.925 
Toseland    13.086 
ProMoST     13.583 
Dawson      13.086 
Bjellqvist  13.086 
Wikipedia   13.568 
Rodwell     12.603 
Grimsley    13.13 
Solomon     13.583 
Lehninger   13.495 
Nozaki      13.086 
DTASelect   13.086 
Thurlkill   13.086 
EMBOSS      13.583 
Sillero     13.086 
Patrickios  12.31 
IPC_peptide 13.598 
IPC2_peptide  12.588 
IPC2.peptide.svr19  9.263 
  
 
Peptides (in silico  digests for buttom-up proteomics) 
Below you can find 
in silico  digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
 
  
    Try ESI  
     
   
 
  
    ChTry ESI  
     
   
 
  
    ArgC ESI  
     
   
 
  
    LysN ESI  
     
   
 
  
    TryLysC ESI  
     
   
 
  
    Try MALDI  
     
   
 
  
    ChTry MALDI  
     
   
 
  
    ArgC MALDI  
     
   
 
  
    LysN MALDI  
     
   
 
  
    TryLysC MALDI  
     
   
 
  
    Try LTQ  
     
   
 
  
    ChTry LTQ  
     
   
 
  
    ArgC LTQ  
     
   
 
  
    LysN LTQ  
     
   
 
  
    TryLysC LTQ  
     
   
 
  
    Try MSlow  
     
   
 
  
    ChTry MSlow  
     
   
 
  
    ArgC MSlow  
     
   
 
  
    LysN MSlow  
     
   
 
  
    TryLysC MSlow  
     
   
 
  
    Try MShigh  
     
   
 
  
    ChTry MShigh  
     
   
 
  
    ArgC MShigh  
     
   
 
  
    LysN MShigh  
     
   
 
  
    TryLysC MShigh  
     
   
   
  
                       
General Statistics 
    
      
        Number of major isoforms
 
        Number of additional isoforms
 
        Number of all proteins
         
        Number of amino acids
 
        Min. Seq. Length
 
        Max. Seq. Length
 
        Avg. Seq. Length
 
        Avg. Mol. Weight
 
       
     
    
      
        3588 
 
        
        0
 
        
        3588 
         
        1168738
 
        28
 
        4570
 
        325.7
 
        35.54
 
                
     
  
 
    Amino acid frequency 
  
  
    
      
        Ala
 
        Cys
 
        Asp
 
        Glu
 
        Phe
 
        Gly
 
        His
 
        Ile
 
        Lys
 
        Leu
 
       
     
    
                 
        12.7 ± 0.059
1.011 ± 0.016
 
        5.349 ± 0.031
5.498 ± 0.038
 
        3.498 ± 0.028
8.075 ± 0.041
 
        2.084 ± 0.018
4.513 ± 0.029
       
        3.641 ± 0.043
11.59 ± 0.064
         
                
     
  
  
  
      
          
        Met
         
        Asn
 
        Gln
 
        Pro
         
        Arg
 
        Ser
 
        Thr
 
        Val
 
        Trp
 
        Tyr
  
       
     
    
                 
        2.441 ± 0.019
2.879 ± 0.027
 
        4.949 ± 0.032
4.429 ± 0.027
 
        6.819 ± 0.047
5.544 ± 0.037
 
        4.284 ± 0.035
6.754 ± 0.038
       
        1.454 ± 0.021
2.487 ± 0.022
         
                
     
  
  
  
Note:  For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level 
Most of the basic statistics you can see at this page can be downloaded from this CSV file  
For dipeptide frequency statistics click here