Xenophilus sp. AP218F
Average proteome isoelectric point is 6.81
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3588 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A254VN13|A0A254VN13_9BURK Uncharacterized protein OS=Xenophilus sp. AP218F OX=2014876 GN=CEK28_02895 PE=4 SV=1
MM1 pKa = 7.55 TDD3 pKa = 2.83 VATEE7 pKa = 4.24 MPCPINFTDD16 pKa = 3.82 SACAKK21 pKa = 9.61 VQDD24 pKa = 5.36 LIAEE28 pKa = 4.34 EE29 pKa = 4.65 GNPDD33 pKa = 2.99 LKK35 pKa = 11.14 LRR37 pKa = 11.84 VFVTGGGCSGFQYY50 pKa = 10.94 GFTFDD55 pKa = 4.65 EE56 pKa = 4.88 IANEE60 pKa = 4.13 DD61 pKa = 3.33 DD62 pKa = 3.66 TAIEE66 pKa = 4.11 RR67 pKa = 11.84 QGVTFLVDD75 pKa = 3.46 PMSYY79 pKa = 10.19 QYY81 pKa = 11.57 LVGAEE86 pKa = 3.7 IDD88 pKa = 3.88 YY89 pKa = 11.15 QEE91 pKa = 4.55 SLEE94 pKa = 4.16 GSQFVIRR101 pKa = 11.84 NPNATTTCGCGSSFSVV117 pKa = 3.54
Molecular weight: 12.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.82
IPC2_protein 3.77
IPC_protein 3.706
Toseland 3.516
ProMoST 3.872
Dawson 3.694
Bjellqvist 3.859
Wikipedia 3.63
Rodwell 3.541
Grimsley 3.427
Solomon 3.668
Lehninger 3.63
Nozaki 3.821
DTASelect 3.999
Thurlkill 3.567
EMBOSS 3.643
Sillero 3.821
Patrickios 0.299
IPC_peptide 3.668
IPC2_peptide 3.795
IPC2.peptide.svr19 3.759
Protein with the highest isoelectric point:
>tr|A0A254VL04|A0A254VL04_9BURK Phosphatase NudJ OS=Xenophilus sp. AP218F OX=2014876 GN=nudJ PE=3 SV=1
MM1 pKa = 7.75 LFRR4 pKa = 11.84 KK5 pKa = 10.21 RR6 pKa = 11.84 SWQAFRR12 pKa = 11.84 LRR14 pKa = 11.84 PRR16 pKa = 11.84 EE17 pKa = 3.96 SPRR20 pKa = 11.84 RR21 pKa = 11.84 AVRR24 pKa = 11.84 PPTNPRR30 pKa = 11.84 CRR32 pKa = 11.84 EE33 pKa = 3.81 LPSRR37 pKa = 11.84 LRR39 pKa = 11.84 RR40 pKa = 11.84 SRR42 pKa = 11.84 LRR44 pKa = 11.84 RR45 pKa = 11.84 SRR47 pKa = 11.84 LRR49 pKa = 11.84 RR50 pKa = 11.84 SRR52 pKa = 11.84 LRR54 pKa = 11.84 RR55 pKa = 11.84 SRR57 pKa = 11.84 LRR59 pKa = 11.84 RR60 pKa = 11.84 SRR62 pKa = 11.84 LRR64 pKa = 11.84 RR65 pKa = 11.84 SRR67 pKa = 11.84 LRR69 pKa = 11.84 RR70 pKa = 11.84 SRR72 pKa = 11.84 LRR74 pKa = 11.84 RR75 pKa = 11.84 SRR77 pKa = 11.84 LRR79 pKa = 11.84 RR80 pKa = 11.84 SRR82 pKa = 11.84 LRR84 pKa = 11.84 QSPLRR89 pKa = 11.84 QSRR92 pKa = 11.84 LRR94 pKa = 11.84 QSRR97 pKa = 11.84 LRR99 pKa = 11.84 QSQRR103 pKa = 11.84 HH104 pKa = 4.82 PLRR107 pKa = 11.84 RR108 pKa = 11.84 RR109 pKa = 11.84 PRR111 pKa = 11.84 QKK113 pKa = 10.51 ARR115 pKa = 11.84 IRR117 pKa = 11.84 RR118 pKa = 11.84 RR119 pKa = 3.23
Molecular weight: 15.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.527
IPC2_protein 11.316
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.568
Rodwell 12.603
Grimsley 13.13
Solomon 13.583
Lehninger 13.495
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.31
IPC_peptide 13.598
IPC2_peptide 12.588
IPC2.peptide.svr19 9.263
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3588
0
3588
1168738
28
4570
325.7
35.54
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.7 ± 0.059
1.011 ± 0.016
5.349 ± 0.031
5.498 ± 0.038
3.498 ± 0.028
8.075 ± 0.041
2.084 ± 0.018
4.513 ± 0.029
3.641 ± 0.043
11.59 ± 0.064
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.441 ± 0.019
2.879 ± 0.027
4.949 ± 0.032
4.429 ± 0.027
6.819 ± 0.047
5.544 ± 0.037
4.284 ± 0.035
6.754 ± 0.038
1.454 ± 0.021
2.487 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here