Lake Sarah-associated circular virus-32

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 7.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A126GAA6|A0A126GAA6_9VIRU ATP-dependent helicase Rep OS=Lake Sarah-associated circular virus-32 OX=1685760 PE=3 SV=1
MM1 pKa = 7.53EE2 pKa = 5.37FDD4 pKa = 3.45KK5 pKa = 10.95EE6 pKa = 3.78FRR8 pKa = 11.84IPFRR12 pKa = 11.84QTTRR16 pKa = 11.84IVVTGEE22 pKa = 3.62AGNQYY27 pKa = 10.21FVLKK31 pKa = 10.51DD32 pKa = 3.27YY33 pKa = 11.06SRR35 pKa = 11.84WGAAIAADD43 pKa = 3.9TRR45 pKa = 11.84APTALGLLGQTDD57 pKa = 3.78TDD59 pKa = 4.48DD60 pKa = 4.72NCPQLQDD67 pKa = 3.72VYY69 pKa = 10.62SQLVNYY75 pKa = 7.34EE76 pKa = 3.82YY77 pKa = 11.2CKK79 pKa = 9.57IHH81 pKa = 6.61AVNEE85 pKa = 3.94EE86 pKa = 3.95FRR88 pKa = 11.84VVDD91 pKa = 3.47YY92 pKa = 9.93EE93 pKa = 4.24VRR95 pKa = 11.84GSGTTVALSRR105 pKa = 11.84NSPHH109 pKa = 5.94FTRR112 pKa = 11.84DD113 pKa = 2.98PSAQLLWKK121 pKa = 8.98LTTNGTEE128 pKa = 4.12EE129 pKa = 4.37SRR131 pKa = 11.84LLTAAAVDD139 pKa = 3.86GADD142 pKa = 3.8FMRR145 pKa = 11.84ALAHH149 pKa = 5.44TRR151 pKa = 11.84RR152 pKa = 11.84GTSFTTRR159 pKa = 11.84LPTEE163 pKa = 4.0MKK165 pKa = 9.82MMRR168 pKa = 11.84GQAGRR173 pKa = 11.84LLLSWTGRR181 pKa = 11.84EE182 pKa = 3.88ATPNITINGSILSNNANNLGPTGSSTASSAFNIRR216 pKa = 11.84NTSAGSGIVDD226 pKa = 4.61FSAHH230 pKa = 6.62RR231 pKa = 11.84SNIFYY236 pKa = 9.64WGKK239 pKa = 9.01GQIYY243 pKa = 10.61NMGGQCDD250 pKa = 3.81VNNYY254 pKa = 6.27QSKK257 pKa = 10.64YY258 pKa = 9.81YY259 pKa = 9.49MATATPYY266 pKa = 10.81AILPSNEE273 pKa = 3.36ARR275 pKa = 11.84NDD277 pKa = 2.88ISFPRR282 pKa = 11.84IFSCTPLIEE291 pKa = 4.44SVTAFNTEE299 pKa = 4.17RR300 pKa = 11.84VWKK303 pKa = 8.98IQNITTLIVKK313 pKa = 10.01ARR315 pKa = 11.84GYY317 pKa = 10.92VPTKK321 pKa = 10.42ALSSLKK327 pKa = 10.71FEE329 pKa = 4.38TQFLSVNDD337 pKa = 4.36DD338 pKa = 4.12DD339 pKa = 5.92EE340 pKa = 5.23PMSKK344 pKa = 9.99QIKK347 pKa = 10.01LIEE350 pKa = 4.03

Molecular weight: 38.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A126GAA6|A0A126GAA6_9VIRU ATP-dependent helicase Rep OS=Lake Sarah-associated circular virus-32 OX=1685760 PE=3 SV=1
MM1 pKa = 7.9LIPIRR6 pKa = 11.84RR7 pKa = 11.84RR8 pKa = 11.84SLRR11 pKa = 11.84IHH13 pKa = 6.83LWIQPSAPPCGQSKK27 pKa = 8.84TIKK30 pKa = 9.75PRR32 pKa = 11.84PSQPKK37 pKa = 9.02LSVRR41 pKa = 11.84MTSFSSKK48 pKa = 9.12RR49 pKa = 11.84WCFTLNNYY57 pKa = 9.85SPEE60 pKa = 3.88EE61 pKa = 3.94HH62 pKa = 6.54QALQLAFKK70 pKa = 10.67ASTVKK75 pKa = 10.63YY76 pKa = 10.17AVIGIEE82 pKa = 4.17KK83 pKa = 10.04GEE85 pKa = 4.34SGTPHH90 pKa = 6.1LQGFVVFKK98 pKa = 10.85KK99 pKa = 8.33MQRR102 pKa = 11.84LSALKK107 pKa = 9.99KK108 pKa = 9.38LNPRR112 pKa = 11.84AHH114 pKa = 6.04YY115 pKa = 10.06EE116 pKa = 4.03EE117 pKa = 5.24AKK119 pKa = 9.65GTSQQASTYY128 pKa = 7.74CQKK131 pKa = 11.01EE132 pKa = 4.42GIFEE136 pKa = 4.22EE137 pKa = 5.56FGTCPLEE144 pKa = 4.04PTAIASNASNKK155 pKa = 9.87KK156 pKa = 8.99NEE158 pKa = 4.27QYY160 pKa = 10.73RR161 pKa = 11.84KK162 pKa = 10.72AMDD165 pKa = 3.79LAKK168 pKa = 10.81LGDD171 pKa = 4.12FSAIEE176 pKa = 4.04QEE178 pKa = 4.91WPGLWLRR185 pKa = 11.84HH186 pKa = 5.34RR187 pKa = 11.84NAFLSAPRR195 pKa = 11.84DD196 pKa = 3.52FGIRR200 pKa = 11.84PKK202 pKa = 11.0DD203 pKa = 3.71LDD205 pKa = 4.09YY206 pKa = 11.68LPGIWIWGPAGVGKK220 pKa = 10.45SRR222 pKa = 11.84LARR225 pKa = 11.84EE226 pKa = 4.27VFPLSYY232 pKa = 10.57EE233 pKa = 3.72KK234 pKa = 10.68RR235 pKa = 11.84LNKK238 pKa = 9.59WFDD241 pKa = 3.35GYY243 pKa = 10.8SAQKK247 pKa = 10.53AVLIEE252 pKa = 5.91DD253 pKa = 3.63IDD255 pKa = 3.61QSHH258 pKa = 6.88RR259 pKa = 11.84YY260 pKa = 8.83ISHH263 pKa = 7.52DD264 pKa = 3.55LKK266 pKa = 10.8IWTDD270 pKa = 3.37RR271 pKa = 11.84YY272 pKa = 10.97SFPAEE277 pKa = 3.76IKK279 pKa = 10.68GGAIQCRR286 pKa = 11.84PEE288 pKa = 3.95HH289 pKa = 6.55VLITSQYY296 pKa = 8.7HH297 pKa = 5.64IHH299 pKa = 7.54DD300 pKa = 3.56IWDD303 pKa = 3.8DD304 pKa = 3.72VQTRR308 pKa = 11.84EE309 pKa = 4.5AIGRR313 pKa = 11.84RR314 pKa = 11.84CFVIYY319 pKa = 9.81IDD321 pKa = 3.7KK322 pKa = 9.24ATPNAYY328 pKa = 9.41KK329 pKa = 10.64SSLSALNHH337 pKa = 6.93LIRR340 pKa = 11.84SRR342 pKa = 11.84APVGEE347 pKa = 4.35CTVVPVAPSDD357 pKa = 3.86VSASAHH363 pKa = 5.42VAPSPPVATLPVLFPDD379 pKa = 4.43PSDD382 pKa = 4.97LSVLDD387 pKa = 3.87

Molecular weight: 43.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

737

350

387

368.5

41.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.548 ± 0.015

1.493 ± 0.228

4.749 ± 0.071

5.02 ± 0.107

4.206 ± 0.239

5.834 ± 0.481

2.171 ± 0.672

6.242 ± 0.344

5.292 ± 1.031

7.87 ± 0.288

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.628 ± 0.43

4.478 ± 1.368

5.97 ± 1.287

4.206 ± 0.135

6.513 ± 0.038

8.548 ± 0.358

6.649 ± 2.003

5.156 ± 0.009

1.9 ± 0.308

3.528 ± 0.122
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski