Beihai weivirus-like virus 21

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KL93|A0A1L3KL93_9VIRU RNA-dependent RNA polymerase OS=Beihai weivirus-like virus 21 OX=1922750 PE=4 SV=1
MM1 pKa = 7.39FGFGRR6 pKa = 11.84PALCSLCHH14 pKa = 6.6ADD16 pKa = 4.16EE17 pKa = 4.71LADD20 pKa = 3.76LSVPVGSDD28 pKa = 2.66NVYY31 pKa = 10.73RR32 pKa = 11.84KK33 pKa = 9.51MIIPANCSRR42 pKa = 11.84CRR44 pKa = 11.84HH45 pKa = 5.52SSVLSRR51 pKa = 11.84KK52 pKa = 9.28RR53 pKa = 11.84KK54 pKa = 8.65YY55 pKa = 9.48KK56 pKa = 10.28GHH58 pKa = 5.77MVYY61 pKa = 10.7LPARR65 pKa = 11.84LVADD69 pKa = 4.33ARR71 pKa = 11.84ALSAKK76 pKa = 10.28CGATTLWGMKK86 pKa = 8.79PANADD91 pKa = 3.57LQLVNLLSAEE101 pKa = 4.04RR102 pKa = 11.84DD103 pKa = 3.43GRR105 pKa = 11.84RR106 pKa = 11.84ANALLTVGLHH116 pKa = 6.63AIKK119 pKa = 10.7DD120 pKa = 3.81EE121 pKa = 4.06EE122 pKa = 4.58TFRR125 pKa = 11.84SGHH128 pKa = 4.68TCCVIDD134 pKa = 5.74ALIKK138 pKa = 10.83CLTDD142 pKa = 3.28QPRR145 pKa = 11.84NATSWRR151 pKa = 11.84RR152 pKa = 11.84VKK154 pKa = 8.79EE155 pKa = 4.56TPAKK159 pKa = 10.5AILDD163 pKa = 4.0GGKK166 pKa = 10.29GATDD170 pKa = 3.8DD171 pKa = 4.21ASVSGGAVSTGDD183 pKa = 3.96DD184 pKa = 3.51SSSIASAEE192 pKa = 3.83SAEE195 pKa = 4.26RR196 pKa = 11.84VNSQQSAFRR205 pKa = 11.84DD206 pKa = 3.6GTTIKK211 pKa = 9.6STVVEE216 pKa = 4.39APGTASTSGQAEE228 pKa = 4.28GEE230 pKa = 4.27EE231 pKa = 4.27VGARR235 pKa = 11.84VAQARR240 pKa = 11.84FPSMSEE246 pKa = 3.67KK247 pKa = 10.32EE248 pKa = 4.31VYY250 pKa = 10.45LFSNDD255 pKa = 3.1PRR257 pKa = 11.84NLEE260 pKa = 3.93AANTLRR266 pKa = 11.84NKK268 pKa = 10.3GVGVHH273 pKa = 5.48QPSEE277 pKa = 4.37KK278 pKa = 9.61EE279 pKa = 3.6AKK281 pKa = 10.38ARR283 pKa = 11.84DD284 pKa = 3.84EE285 pKa = 5.04IIAALCAQVFTEE297 pKa = 4.64RR298 pKa = 11.84EE299 pKa = 4.01CGKK302 pKa = 10.96AMMGYY307 pKa = 10.48EE308 pKa = 4.45SLTKK312 pKa = 9.68TALPRR317 pKa = 11.84KK318 pKa = 9.77LSEE321 pKa = 3.98QQKK324 pKa = 8.61EE325 pKa = 4.15QWQLDD330 pKa = 3.53AMKK333 pKa = 9.97EE334 pKa = 4.09AEE336 pKa = 4.51GDD338 pKa = 3.64GLSYY342 pKa = 11.33SKK344 pKa = 10.82FVDD347 pKa = 3.35AFVKK351 pKa = 10.9AEE353 pKa = 3.97VSAKK357 pKa = 10.0PKK359 pKa = 9.53PRR361 pKa = 11.84PIANHH366 pKa = 5.93KK367 pKa = 9.6EE368 pKa = 3.51IRR370 pKa = 11.84LCALAKK376 pKa = 9.5VAWVYY381 pKa = 10.89EE382 pKa = 3.9YY383 pKa = 11.83VLFHH387 pKa = 6.91KK388 pKa = 9.94FKK390 pKa = 10.94KK391 pKa = 10.0MSIKK395 pKa = 10.4HH396 pKa = 4.18RR397 pKa = 11.84TKK399 pKa = 10.6RR400 pKa = 11.84EE401 pKa = 3.95ALSDD405 pKa = 3.24IAGALSGMRR414 pKa = 11.84NGRR417 pKa = 11.84WCEE420 pKa = 3.56NDD422 pKa = 2.77LTAFEE427 pKa = 4.84FGISAKK433 pKa = 10.47LKK435 pKa = 9.13EE436 pKa = 4.61CEE438 pKa = 4.19CVILRR443 pKa = 11.84HH444 pKa = 5.67IASHH448 pKa = 6.54IGVEE452 pKa = 4.35DD453 pKa = 3.42VGPSLFEE460 pKa = 3.65RR461 pKa = 11.84VVNDD465 pKa = 3.42RR466 pKa = 11.84TKK468 pKa = 10.24ACVWSMRR475 pKa = 11.84YY476 pKa = 9.41KK477 pKa = 10.98DD478 pKa = 3.28EE479 pKa = 4.28TGAQRR484 pKa = 11.84KK485 pKa = 8.48FKK487 pKa = 11.2LEE489 pKa = 3.9LPNAMRR495 pKa = 11.84EE496 pKa = 4.17SGDD499 pKa = 3.52RR500 pKa = 11.84LTSSGNFLQNLIAWGSFLVEE520 pKa = 4.42PGHH523 pKa = 5.51VSKK526 pKa = 10.92AIEE529 pKa = 4.24SLVRR533 pKa = 11.84THH535 pKa = 6.7GEE537 pKa = 3.71KK538 pKa = 10.69LFYY541 pKa = 10.62TSARR545 pKa = 11.84DD546 pKa = 3.68GNKK549 pKa = 9.41YY550 pKa = 10.35LAMLVFEE557 pKa = 5.53GDD559 pKa = 3.46DD560 pKa = 3.26TLGRR564 pKa = 11.84LEE566 pKa = 4.11EE567 pKa = 4.54PVWEE571 pKa = 4.65AYY573 pKa = 10.11RR574 pKa = 11.84SGSEE578 pKa = 3.71TSIADD583 pKa = 3.76DD584 pKa = 4.63FFLRR588 pKa = 11.84WGWNPKK594 pKa = 10.11LSWKK598 pKa = 9.15ATTGHH603 pKa = 7.14DD604 pKa = 3.55YY605 pKa = 11.49ARR607 pKa = 11.84VVGYY611 pKa = 10.42DD612 pKa = 3.3VLIKK616 pKa = 10.92DD617 pKa = 4.11GVAVKK622 pKa = 10.85DD623 pKa = 3.38GDD625 pKa = 4.17SYY627 pKa = 10.45VACPEE632 pKa = 4.01MKK634 pKa = 10.32RR635 pKa = 11.84LLTTKK640 pKa = 9.52QWTTSSVTPEE650 pKa = 3.74EE651 pKa = 4.83LKK653 pKa = 8.89TCNRR657 pKa = 11.84IFAATLAADD666 pKa = 4.67FTRR669 pKa = 11.84VEE671 pKa = 3.99PFYY674 pKa = 11.51AFLKK678 pKa = 10.8SMYY681 pKa = 9.47EE682 pKa = 4.05SNRR685 pKa = 11.84GGKK688 pKa = 8.89NVSDD692 pKa = 3.39EE693 pKa = 4.25KK694 pKa = 11.24VRR696 pKa = 11.84EE697 pKa = 4.23FYY699 pKa = 11.02LATTGEE705 pKa = 4.27LPEE708 pKa = 5.34HH709 pKa = 6.62GSSKK713 pKa = 10.9LNDD716 pKa = 3.11IEE718 pKa = 4.65FPEE721 pKa = 4.36FNGTGSEE728 pKa = 3.87AWKK731 pKa = 10.0EE732 pKa = 3.79LARR735 pKa = 11.84VSCGDD740 pKa = 3.37FTDD743 pKa = 5.33AEE745 pKa = 4.36WATACAQPPHH755 pKa = 6.97DD756 pKa = 3.92RR757 pKa = 11.84HH758 pKa = 6.03GADD761 pKa = 4.14LAVGMPASWLAA772 pKa = 3.37

Molecular weight: 85.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KL93|A0A1L3KL93_9VIRU RNA-dependent RNA polymerase OS=Beihai weivirus-like virus 21 OX=1922750 PE=4 SV=1
MM1 pKa = 7.47ARR3 pKa = 11.84RR4 pKa = 11.84RR5 pKa = 11.84NTRR8 pKa = 11.84VTNGRR13 pKa = 11.84SGRR16 pKa = 11.84ARR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84PNAKK24 pKa = 9.34GASRR28 pKa = 11.84SVATRR33 pKa = 11.84VLATGVGASVPKK45 pKa = 10.76AFGSVAGYY53 pKa = 10.53GLHH56 pKa = 6.79CWDD59 pKa = 4.74AKK61 pKa = 10.73HH62 pKa = 6.29LAHH65 pKa = 6.98LPLPRR70 pKa = 11.84AVGPYY75 pKa = 5.61TTIRR79 pKa = 11.84ATRR82 pKa = 11.84RR83 pKa = 11.84IQISTHH89 pKa = 5.8ANVIGTFQCGSNMPEE104 pKa = 3.7QHH106 pKa = 6.53FDD108 pKa = 2.63WSEE111 pKa = 3.56AVMVTDD117 pKa = 4.1VVSGSAITAPSNAQLITLDD136 pKa = 4.14LGGLGDD142 pKa = 4.41AATLVPSALSVQIMCPTALQSASGIVYY169 pKa = 10.04AGVMNTQTAIAGRR182 pKa = 11.84NEE184 pKa = 3.81TWDD187 pKa = 3.25SYY189 pKa = 9.48MEE191 pKa = 4.73KK192 pKa = 10.08FVQFQNPRR200 pKa = 11.84LLAASKK206 pKa = 10.15LALRR210 pKa = 11.84GVQINSYY217 pKa = 8.46PLNMTEE223 pKa = 4.06VSKK226 pKa = 8.46FTPLCKK232 pKa = 10.18LADD235 pKa = 3.61SSAFTLDD242 pKa = 3.34GASIEE247 pKa = 4.24PAGWAPIVIFNPSGASLEE265 pKa = 4.19LLITMEE271 pKa = 3.65FRR273 pKa = 11.84VRR275 pKa = 11.84FDD277 pKa = 4.02LDD279 pKa = 3.43HH280 pKa = 7.02PASASHH286 pKa = 5.36THH288 pKa = 6.24HH289 pKa = 6.96PVASDD294 pKa = 3.75ALWDD298 pKa = 3.92RR299 pKa = 11.84MVKK302 pKa = 10.36GAVALGNGVVDD313 pKa = 3.66IADD316 pKa = 3.59VVANTGMAVGRR327 pKa = 11.84AVAVGSRR334 pKa = 11.84LAAASRR340 pKa = 11.84AMPALAVV347 pKa = 3.56

Molecular weight: 36.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1119

347

772

559.5

60.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.332 ± 1.49

2.055 ± 0.507

5.094 ± 0.595

5.987 ± 2.068

3.485 ± 0.339

7.507 ± 0.316

2.324 ± 0.152

3.485 ± 0.47

5.451 ± 1.766

8.132 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.413 ± 0.425

3.485 ± 0.308

4.111 ± 0.604

2.324 ± 0.314

6.613 ± 0.17

8.132 ± 0.126

5.898 ± 0.41

7.328 ± 1.063

1.787 ± 0.195

2.055 ± 0.345
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski