Hubei sobemo-like virus 29

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KEH6|A0A1L3KEH6_9VIRU RNA-directed RNA polymerase OS=Hubei sobemo-like virus 29 OX=1923215 PE=4 SV=1
MM1 pKa = 7.63SGGEE5 pKa = 3.94PVRR8 pKa = 11.84FDD10 pKa = 3.86GFPEE14 pKa = 4.41EE15 pKa = 4.17YY16 pKa = 10.1RR17 pKa = 11.84EE18 pKa = 4.07QFEE21 pKa = 4.25KK22 pKa = 10.74AARR25 pKa = 11.84GYY27 pKa = 8.9GWPKK31 pKa = 9.88FGHH34 pKa = 5.69AAEE37 pKa = 4.48LRR39 pKa = 11.84SLHH42 pKa = 5.18YY43 pKa = 10.25HH44 pKa = 6.29ASLRR48 pKa = 11.84DD49 pKa = 3.55KK50 pKa = 10.37TRR52 pKa = 11.84SDD54 pKa = 3.64GPSRR58 pKa = 11.84EE59 pKa = 3.86ARR61 pKa = 11.84EE62 pKa = 4.46AIVQGVAEE70 pKa = 4.58QYY72 pKa = 11.4SSATWSIPEE81 pKa = 4.55DD82 pKa = 3.55FLQFSHH88 pKa = 7.12FEE90 pKa = 4.0RR91 pKa = 11.84VVKK94 pKa = 10.76NLDD97 pKa = 3.26WTSSPGYY104 pKa = 9.12PYY106 pKa = 9.87MRR108 pKa = 11.84RR109 pKa = 11.84APTNRR114 pKa = 11.84ILFNVNSIGVPDD126 pKa = 4.49PDD128 pKa = 3.34KK129 pKa = 11.29VSWMWEE135 pKa = 3.99VVQNQIRR142 pKa = 11.84DD143 pKa = 3.72RR144 pKa = 11.84VSDD147 pKa = 4.72PIRR150 pKa = 11.84LFIKK154 pKa = 10.71AEE156 pKa = 3.69PHH158 pKa = 5.74KK159 pKa = 10.7LKK161 pKa = 10.66KK162 pKa = 10.79LEE164 pKa = 4.4DD165 pKa = 3.18GRR167 pKa = 11.84YY168 pKa = 9.05RR169 pKa = 11.84LISSVSVVDD178 pKa = 3.47QLIDD182 pKa = 3.25HH183 pKa = 6.43MLFGDD188 pKa = 3.76MNEE191 pKa = 4.25SLVEE195 pKa = 3.74NWLNVPSKK203 pKa = 10.59IGWSHH208 pKa = 6.68LWGGWRR214 pKa = 11.84SIPRR218 pKa = 11.84DD219 pKa = 3.02WVAVDD224 pKa = 3.53KK225 pKa = 11.49SSWDD229 pKa = 2.93WTMQSWVCEE238 pKa = 3.92MVLEE242 pKa = 4.32VRR244 pKa = 11.84LRR246 pKa = 11.84LCSNPSQEE254 pKa = 4.44WLDD257 pKa = 3.68LAQWRR262 pKa = 11.84YY263 pKa = 10.18RR264 pKa = 11.84EE265 pKa = 4.5LFLNPLFVTSGGWILRR281 pKa = 11.84QMRR284 pKa = 11.84PGVMKK289 pKa = 10.25SGCVNTIADD298 pKa = 3.64NSIAQSILDD307 pKa = 3.37VRR309 pKa = 11.84VCYY312 pKa = 10.0EE313 pKa = 4.38LGLSPEE319 pKa = 4.47PLMSLGDD326 pKa = 3.42DD327 pKa = 4.13TYY329 pKa = 10.76QKK331 pKa = 10.6KK332 pKa = 9.02RR333 pKa = 11.84AAMGEE338 pKa = 4.02YY339 pKa = 10.41LEE341 pKa = 5.67RR342 pKa = 11.84LSQYY346 pKa = 11.16AILKK350 pKa = 9.12QVVEE354 pKa = 4.27APEE357 pKa = 3.91FGGFRR362 pKa = 11.84FLGPKK367 pKa = 9.01QVEE370 pKa = 3.76PLYY373 pKa = 10.25KK374 pKa = 10.37GKK376 pKa = 9.5HH377 pKa = 5.29AYY379 pKa = 9.17ILLHH383 pKa = 6.3VDD385 pKa = 3.49PEE387 pKa = 4.49VLPEE391 pKa = 4.29LAPSYY396 pKa = 10.31QLLYY400 pKa = 10.38HH401 pKa = 6.68RR402 pKa = 11.84SAYY405 pKa = 10.16RR406 pKa = 11.84DD407 pKa = 3.34MMEE410 pKa = 6.26DD411 pKa = 2.49IFLRR415 pKa = 11.84MGQEE419 pKa = 3.42IVPRR423 pKa = 11.84RR424 pKa = 11.84TRR426 pKa = 11.84DD427 pKa = 3.61LIFDD431 pKa = 4.62GYY433 pKa = 11.43

Molecular weight: 50.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KEH6|A0A1L3KEH6_9VIRU RNA-directed RNA polymerase OS=Hubei sobemo-like virus 29 OX=1923215 PE=4 SV=1
MM1 pKa = 7.49GNRR4 pKa = 11.84VAVRR8 pKa = 11.84RR9 pKa = 11.84LEE11 pKa = 4.26DD12 pKa = 3.73EE13 pKa = 4.39EE14 pKa = 4.53TGSTGSGASPVYY26 pKa = 10.49AATTSPDD33 pKa = 3.01PNAWVNGVIWWCSKK47 pKa = 11.11ALLALLMVVTTAIVVYY63 pKa = 9.9RR64 pKa = 11.84WMAYY68 pKa = 9.39AVRR71 pKa = 11.84SATRR75 pKa = 11.84KK76 pKa = 9.38LRR78 pKa = 11.84SHH80 pKa = 5.9MEE82 pKa = 4.14EE83 pKa = 4.04IYY85 pKa = 11.15SLLDD89 pKa = 3.56YY90 pKa = 10.62ISPSEE95 pKa = 4.22TEE97 pKa = 3.88AEE99 pKa = 3.92RR100 pKa = 11.84EE101 pKa = 4.05EE102 pKa = 4.51KK103 pKa = 10.83VEE105 pKa = 3.81NVYY108 pKa = 10.45VRR110 pKa = 11.84VRR112 pKa = 11.84SAIEE116 pKa = 4.02DD117 pKa = 3.71LPIPSLLLTPSSDD130 pKa = 2.82WVFLAFGVVTLLVGCYY146 pKa = 9.44VSMRR150 pKa = 11.84SFGRR154 pKa = 11.84VSRR157 pKa = 11.84NLVHH161 pKa = 7.93RR162 pKa = 11.84IRR164 pKa = 11.84GIQYY168 pKa = 7.68EE169 pKa = 4.36SVRR172 pKa = 11.84EE173 pKa = 4.05GSSFRR178 pKa = 11.84SAKK181 pKa = 9.82VPAYY185 pKa = 10.1QIAVMEE191 pKa = 5.0AGLLSDD197 pKa = 2.89THH199 pKa = 7.77IGYY202 pKa = 9.6GCRR205 pKa = 11.84YY206 pKa = 9.29CDD208 pKa = 3.64YY209 pKa = 10.81LVLPRR214 pKa = 11.84HH215 pKa = 5.44VLEE218 pKa = 4.82KK219 pKa = 10.93DD220 pKa = 3.11GALVTTVLLKK230 pKa = 11.07GPLSKK235 pKa = 11.17VMTNISPVQSRR246 pKa = 11.84VIDD249 pKa = 3.98DD250 pKa = 4.73LVYY253 pKa = 10.72CYY255 pKa = 10.6LEE257 pKa = 4.34PKK259 pKa = 8.63TWSCLGAAKK268 pKa = 10.27GKK270 pKa = 8.66WANKK274 pKa = 9.5LMSLHH279 pKa = 5.25VTCTGIQGQTAGRR292 pKa = 11.84LARR295 pKa = 11.84TAIRR299 pKa = 11.84WMMSYY304 pKa = 9.67TGTTIEE310 pKa = 4.31GMSGAAYY317 pKa = 9.02EE318 pKa = 4.5SQGEE322 pKa = 3.91IHH324 pKa = 7.28GIHH327 pKa = 6.06QGATGPFNLGISSLLVTQEE346 pKa = 3.84MALLCQVEE354 pKa = 4.46SSLDD358 pKa = 3.53TNKK361 pKa = 10.4DD362 pKa = 2.89AKK364 pKa = 11.16VPLFYY369 pKa = 11.09NQANAKK375 pKa = 9.26LWSQIEE381 pKa = 4.12AMDD384 pKa = 4.17EE385 pKa = 3.78LLEE388 pKa = 4.69RR389 pKa = 11.84YY390 pKa = 10.09SSDD393 pKa = 2.45SWALGGEE400 pKa = 3.93VDD402 pKa = 4.05YY403 pKa = 11.45SKK405 pKa = 11.48KK406 pKa = 10.64LDD408 pKa = 3.93FGEE411 pKa = 4.07EE412 pKa = 3.59AARR415 pKa = 11.84RR416 pKa = 11.84RR417 pKa = 11.84PVSKK421 pKa = 10.33SHH423 pKa = 7.02LNVTLPEE430 pKa = 4.31GGIRR434 pKa = 11.84LANQNNTGSTTDD446 pKa = 3.62YY447 pKa = 11.22VVVTSAEE454 pKa = 4.21KK455 pKa = 10.48EE456 pKa = 3.97FLMEE460 pKa = 4.93LGRR463 pKa = 11.84SAILEE468 pKa = 4.31RR469 pKa = 11.84IEE471 pKa = 4.35ALEE474 pKa = 4.24TKK476 pKa = 10.14VAALGKK482 pKa = 10.48AEE484 pKa = 4.47IPCPHH489 pKa = 7.15CEE491 pKa = 4.13TTCRR495 pKa = 11.84TRR497 pKa = 11.84EE498 pKa = 4.01RR499 pKa = 11.84MDD501 pKa = 2.94HH502 pKa = 6.66HH503 pKa = 6.87IASAHH508 pKa = 5.37PKK510 pKa = 9.45VYY512 pKa = 9.94RR513 pKa = 11.84CQFCEE518 pKa = 4.32VSCRR522 pKa = 11.84TEE524 pKa = 3.97KK525 pKa = 10.86KK526 pKa = 10.21LQNHH530 pKa = 6.72LEE532 pKa = 4.14NNHH535 pKa = 4.76VVKK538 pKa = 10.76KK539 pKa = 10.1EE540 pKa = 3.87SAIPADD546 pKa = 3.62TGKK549 pKa = 10.33SGKK552 pKa = 7.93VVKK555 pKa = 9.74TGSFLGKK562 pKa = 9.9RR563 pKa = 11.84QGSQRR568 pKa = 11.84NNTVSSARR576 pKa = 11.84SSSLSSRR583 pKa = 11.84APPSPCLEE591 pKa = 4.08EE592 pKa = 4.36SLSGLMASQRR602 pKa = 11.84SIEE605 pKa = 4.13SSLKK609 pKa = 10.45KK610 pKa = 10.34LLEE613 pKa = 4.11VMAGRR618 pKa = 11.84NSAMQQSS625 pKa = 3.76

Molecular weight: 68.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1058

433

625

529.0

59.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.994 ± 1.164

1.796 ± 0.53

4.253 ± 1.065

7.278 ± 0.072

2.552 ± 0.836

6.711 ± 0.149

2.268 ± 0.025

4.348 ± 0.165

4.631 ± 0.288

9.735 ± 0.022

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.025 ± 0.127

3.119 ± 0.211

4.631 ± 0.835

3.497 ± 0.401

7.183 ± 0.547

9.546 ± 0.89

4.442 ± 1.438

7.845 ± 0.277

2.457 ± 0.753

3.686 ± 0.286
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski