Capybara microvirus Cap1_SP_188
Average proteome isoelectric point is 5.37
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4V1FVQ8|A0A4V1FVQ8_9VIRU Major capsid protein OS=Capybara microvirus Cap1_SP_188 OX=2585406 PE=3 SV=1
MM1 pKa = 7.79 AKK3 pKa = 9.99 HH4 pKa = 5.98 NYY6 pKa = 9.01 YY7 pKa = 10.71 SIVLRR12 pKa = 11.84 NVQDD16 pKa = 4.17 VEE18 pKa = 5.08 DD19 pKa = 4.02 SDD21 pKa = 5.62 SNFFFDD27 pKa = 3.78 RR28 pKa = 11.84 PFYY31 pKa = 11.3 AEE33 pKa = 3.66 TDD35 pKa = 3.34 AMAKK39 pKa = 10.26 LIFEE43 pKa = 5.01 ASVCSNPVYY52 pKa = 11.33 SMLGDD57 pKa = 5.14 DD58 pKa = 3.83 IALFKK63 pKa = 10.6 IGQFDD68 pKa = 3.95 DD69 pKa = 3.93 EE70 pKa = 4.59 EE71 pKa = 7.08 GIMFNIDD78 pKa = 3.12 GFDD81 pKa = 3.48 EE82 pKa = 4.55 LFIMTGSEE90 pKa = 3.78 ALNVHH95 pKa = 6.06 NEE97 pKa = 3.75 RR98 pKa = 11.84 SEE100 pKa = 4.42 KK101 pKa = 10.23 NGKK104 pKa = 8.96 DD105 pKa = 3.16 GKK107 pKa = 10.16 VANN110 pKa = 4.43
Molecular weight: 12.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.309
IPC2_protein 4.202
IPC_protein 4.151
Toseland 3.948
ProMoST 4.291
Dawson 4.126
Bjellqvist 4.279
Wikipedia 4.037
Rodwell 3.973
Grimsley 3.859
Solomon 4.113
Lehninger 4.075
Nozaki 4.24
DTASelect 4.444
Thurlkill 3.986
EMBOSS 4.05
Sillero 4.266
Patrickios 3.668
IPC_peptide 4.113
IPC2_peptide 4.24
IPC2.peptide.svr19 4.192
Protein with the highest isoelectric point:
>tr|A0A4P8W4H5|A0A4P8W4H5_9VIRU Minor capsid protein OS=Capybara microvirus Cap1_SP_188 OX=2585406 PE=4 SV=1
MM1 pKa = 7.35 ARR3 pKa = 11.84 WKK5 pKa = 8.87 TAEE8 pKa = 4.07 GKK10 pKa = 10.29 KK11 pKa = 5.6 VTRR14 pKa = 11.84 YY15 pKa = 9.83 IGTSGNIQGIPLDD28 pKa = 3.62 AEE30 pKa = 4.44 YY31 pKa = 10.74 FSVGCGHH38 pKa = 7.61 CIACRR43 pKa = 11.84 LSKK46 pKa = 11.04 SKK48 pKa = 10.76 DD49 pKa = 2.78 WANRR53 pKa = 11.84 MTLEE57 pKa = 3.97 AMYY60 pKa = 10.39 HH61 pKa = 5.3 SSCFFLTLTYY71 pKa = 11.05 DD72 pKa = 3.81 DD73 pKa = 3.82 AHH75 pKa = 8.31 LPEE78 pKa = 4.11 QHH80 pKa = 5.99 LQKK83 pKa = 10.98 NGDD86 pKa = 3.2 ISPIHH91 pKa = 6.3 SLNKK95 pKa = 10.02 KK96 pKa = 9.44 HH97 pKa = 6.39 FQDD100 pKa = 3.38 FMKK103 pKa = 10.54 RR104 pKa = 11.84 LRR106 pKa = 11.84 WKK108 pKa = 10.45 FRR110 pKa = 11.84 DD111 pKa = 3.41 VEE113 pKa = 3.84 IRR115 pKa = 11.84 YY116 pKa = 7.41 YY117 pKa = 11.1 ACGEE121 pKa = 4.04 YY122 pKa = 10.64 GEE124 pKa = 4.94 KK125 pKa = 10.42 SMRR128 pKa = 11.84 PHH130 pKa = 4.8 YY131 pKa = 10.08 HH132 pKa = 6.93 AIIFGLPLDD141 pKa = 4.12 RR142 pKa = 11.84 LGDD145 pKa = 4.52 AIYY148 pKa = 10.29 SGCSSGFKK156 pKa = 10.07 VFAYY160 pKa = 9.77 TEE162 pKa = 4.37 LSKK165 pKa = 11.09 LWVFGNHH172 pKa = 5.44 IVAEE176 pKa = 4.48 ANWQTMAYY184 pKa = 7.51 TSRR187 pKa = 11.84 YY188 pKa = 5.63 ITKK191 pKa = 9.0 KK192 pKa = 10.11 QFGIGSKK199 pKa = 9.59 IYY201 pKa = 10.43 EE202 pKa = 4.16 EE203 pKa = 4.28 LGYY206 pKa = 10.22 IPEE209 pKa = 4.36 FSLMSRR215 pKa = 11.84 KK216 pKa = 9.59 PGIGKK221 pKa = 8.81 IWLDD225 pKa = 3.23 ASRR228 pKa = 11.84 YY229 pKa = 9.75 SVLDD233 pKa = 3.71 DD234 pKa = 3.52 KK235 pKa = 11.52 AIYY238 pKa = 10.08 IPGKK242 pKa = 9.53 GKK244 pKa = 10.16 QPIPRR249 pKa = 11.84 YY250 pKa = 9.49 FKK252 pKa = 10.92 EE253 pKa = 3.95 KK254 pKa = 10.79 LKK256 pKa = 10.54 DD257 pKa = 2.88 ICYY260 pKa = 9.96 FCDD263 pKa = 3.16 VDD265 pKa = 5.26 GVINPTDD272 pKa = 3.3 EE273 pKa = 4.71 ALWLEE278 pKa = 4.14 FDD280 pKa = 3.4 DD281 pKa = 3.9 WLRR284 pKa = 11.84 DD285 pKa = 3.72 VGFHH289 pKa = 6.07 QYY291 pKa = 10.72 CEE293 pKa = 4.05 QQMKK297 pKa = 10.56 LDD299 pKa = 3.94 QTTLTYY305 pKa = 10.71 SEE307 pKa = 4.45 VLEE310 pKa = 4.23 NTQYY314 pKa = 11.63 AHH316 pKa = 6.06 EE317 pKa = 4.27 QVASSLGRR325 pKa = 11.84 KK326 pKa = 8.4 GVV328 pKa = 3.16
Molecular weight: 37.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.059
IPC2_protein 7.322
IPC_protein 7.234
Toseland 6.971
ProMoST 7.775
Dawson 7.907
Bjellqvist 8.229
Wikipedia 7.834
Rodwell 7.922
Grimsley 7.044
Solomon 7.966
Lehninger 7.995
Nozaki 8.404
DTASelect 8.039
Thurlkill 8.083
EMBOSS 8.141
Sillero 8.39
Patrickios 3.884
IPC_peptide 7.966
IPC2_peptide 7.366
IPC2.peptide.svr19 7.409
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1461
110
598
292.2
32.9
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.297 ± 0.926
1.3 ± 0.407
7.529 ± 0.607
5.476 ± 0.501
5.339 ± 0.714
7.05 ± 0.521
2.19 ± 0.439
5.681 ± 0.365
5.955 ± 1.456
6.776 ± 0.392
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.396 ± 0.22
5.133 ± 0.95
4.107 ± 1.082
3.901 ± 0.409
4.312 ± 0.443
9.446 ± 2.198
5.065 ± 0.519
5.749 ± 0.755
1.437 ± 0.294
4.86 ± 0.401
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here