Bacteroides sp. CAG:189
Average proteome isoelectric point is 6.39
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3866 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|R5J862|R5J862_9BACE Sensor protein rprX OS=Bacteroides sp. CAG:189 OX=1262737 GN=BN523_02526 PE=4 SV=1
MM1 pKa = 7.38 KK2 pKa = 10.29 FKK4 pKa = 10.77 YY5 pKa = 10.46 YY6 pKa = 10.63 LGAVMLAIILFSFSACDD23 pKa = 3.52 NDD25 pKa = 5.08 DD26 pKa = 5.43 DD27 pKa = 5.35 YY28 pKa = 11.61 MPPNQDD34 pKa = 3.15 IITALKK40 pKa = 9.82 QLYY43 pKa = 9.74 PDD45 pKa = 3.57 VQDD48 pKa = 4.64 IEE50 pKa = 4.4 WSQKK54 pKa = 8.49 GVYY57 pKa = 9.22 YY58 pKa = 10.89 VADD61 pKa = 3.48 CWVNGSEE68 pKa = 4.57 LDD70 pKa = 3.39 VWFDD74 pKa = 4.67 ANANWIMTEE83 pKa = 3.72 MEE85 pKa = 4.33 IFRR88 pKa = 11.84 EE89 pKa = 3.87 QLPAAVNTAYY99 pKa = 10.18 EE100 pKa = 3.96 EE101 pKa = 4.02 SGYY104 pKa = 10.91 GDD106 pKa = 3.41 WVIDD110 pKa = 3.81 NLTKK114 pKa = 10.21 LTFPHH119 pKa = 6.82 KK120 pKa = 10.34 SEE122 pKa = 3.96 EE123 pKa = 4.28 FVFEE127 pKa = 4.24 VQRR130 pKa = 11.84 GAQEE134 pKa = 3.59 RR135 pKa = 11.84 ALYY138 pKa = 9.36 YY139 pKa = 10.12 SAYY142 pKa = 10.44 GGLLLEE148 pKa = 5.25 KK149 pKa = 10.18 DD150 pKa = 3.2 ITDD153 pKa = 4.32 ADD155 pKa = 3.88 DD156 pKa = 3.7 THH158 pKa = 6.82 WPDD161 pKa = 3.57 VEE163 pKa = 4.12
Molecular weight: 18.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.771
IPC2_protein 3.935
IPC_protein 3.91
Toseland 3.706
ProMoST 4.037
Dawson 3.897
Bjellqvist 4.088
Wikipedia 3.821
Rodwell 3.745
Grimsley 3.617
Solomon 3.884
Lehninger 3.846
Nozaki 4.012
DTASelect 4.228
Thurlkill 3.757
EMBOSS 3.834
Sillero 4.037
Patrickios 0.807
IPC_peptide 3.884
IPC2_peptide 4.012
IPC2.peptide.svr19 3.926
Protein with the highest isoelectric point:
>tr|R5JLV2|R5JLV2_9BACE WYL domain-containing protein OS=Bacteroides sp. CAG:189 OX=1262737 GN=BN523_02166 PE=4 SV=1
MM1 pKa = 7.41 GGISPFTRR9 pKa = 11.84 FKK11 pKa = 10.96 VVYY14 pKa = 9.98 SSPSLLKK21 pKa = 10.79 EE22 pKa = 3.81 MASGQRR28 pKa = 11.84 NSEE31 pKa = 3.76 GLYY34 pKa = 8.99 TVAYY38 pKa = 7.97 MPSLNRR44 pKa = 11.84 CPQILALLFTKK55 pKa = 10.51 PSARR59 pKa = 11.84 NTGSAQLWPPSSLTDD74 pKa = 3.22 VKK76 pKa = 11.41 ACFHH80 pKa = 5.87 RR81 pKa = 11.84 QARR84 pKa = 11.84 RR85 pKa = 11.84 PSGSVV90 pKa = 2.71
Molecular weight: 9.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.334
IPC2_protein 9.78
IPC_protein 10.482
Toseland 10.511
ProMoST 10.218
Dawson 10.657
Bjellqvist 10.379
Wikipedia 10.862
Rodwell 10.935
Grimsley 10.716
Solomon 10.73
Lehninger 10.701
Nozaki 10.526
DTASelect 10.365
Thurlkill 10.526
EMBOSS 10.906
Sillero 10.584
Patrickios 10.716
IPC_peptide 10.73
IPC2_peptide 9.502
IPC2.peptide.svr19 8.443
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3866
0
3866
1447579
29
2818
374.4
42.24
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.897 ± 0.035
1.228 ± 0.014
5.54 ± 0.027
6.537 ± 0.034
4.593 ± 0.022
6.941 ± 0.035
1.816 ± 0.017
7.08 ± 0.036
6.555 ± 0.034
8.971 ± 0.037
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.701 ± 0.018
5.252 ± 0.03
3.742 ± 0.02
3.337 ± 0.02
4.48 ± 0.031
6.194 ± 0.031
5.773 ± 0.028
6.474 ± 0.034
1.297 ± 0.016
4.591 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here