Amino acid dipepetide frequency for Thermus phage phiKo

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.772AlaAla: 9.772 ± 2.045
0.592AlaCys: 0.592 ± 0.377
2.369AlaAsp: 2.369 ± 1.157
5.33AlaGlu: 5.33 ± 2.013
2.369AlaPhe: 2.369 ± 0.958
7.403AlaGly: 7.403 ± 1.129
1.777AlaHis: 1.777 ± 0.72
4.738AlaIle: 4.738 ± 1.127
2.961AlaLys: 2.961 ± 1.058
16.287AlaLeu: 16.287 ± 2.075
2.665AlaMet: 2.665 ± 0.824
2.073AlaAsn: 2.073 ± 0.732
4.442AlaPro: 4.442 ± 0.76
4.146AlaGln: 4.146 ± 0.982
6.515AlaArg: 6.515 ± 1.723
4.146AlaSer: 4.146 ± 1.047
7.403AlaThr: 7.403 ± 1.471
12.437AlaVal: 12.437 ± 2.556
2.665AlaTrp: 2.665 ± 0.708
7.107AlaTyr: 7.107 ± 1.532
0.0AlaXaa: 0.0 ± 0.0
Cys
0.0CysAla: 0.0 ± 0.0
0.0CysCys: 0.0 ± 0.0
0.0CysAsp: 0.0 ± 0.0
0.0CysGlu: 0.0 ± 0.0
0.0CysPhe: 0.0 ± 0.0
0.0CysGly: 0.0 ± 0.0
0.0CysHis: 0.0 ± 0.0
0.296CysIle: 0.296 ± 0.272
0.0CysLys: 0.0 ± 0.0
0.592CysLeu: 0.592 ± 0.42
0.0CysMet: 0.0 ± 0.0
0.296CysAsn: 0.296 ± 0.262
0.592CysPro: 0.592 ± 0.378
0.0CysGln: 0.0 ± 0.0
0.296CysArg: 0.296 ± 0.298
0.296CysSer: 0.296 ± 0.296
0.296CysThr: 0.296 ± 0.296
0.296CysVal: 0.296 ± 0.272
0.592CysTrp: 0.592 ± 0.36
0.0CysTyr: 0.0 ± 0.0
0.0CysXaa: 0.0 ± 0.0
Asp
3.85AspAla: 3.85 ± 0.929
0.592AspCys: 0.592 ± 0.399
1.777AspAsp: 1.777 ± 0.563
3.257AspGlu: 3.257 ± 1.002
1.481AspPhe: 1.481 ± 0.734
2.961AspGly: 2.961 ± 1.037
0.0AspHis: 0.0 ± 0.0
1.481AspIle: 1.481 ± 0.622
1.481AspLys: 1.481 ± 0.701
3.553AspLeu: 3.553 ± 1.126
0.888AspMet: 0.888 ± 0.893
0.296AspAsn: 0.296 ± 0.281
2.073AspPro: 2.073 ± 0.654
0.296AspGln: 0.296 ± 0.272
3.257AspArg: 3.257 ± 1.024
2.961AspSer: 2.961 ± 0.897
2.073AspThr: 2.073 ± 1.176
3.257AspVal: 3.257 ± 0.785
2.369AspTrp: 2.369 ± 0.944
2.369AspTyr: 2.369 ± 0.796
0.0AspXaa: 0.0 ± 0.0
Glu
14.214GluAla: 14.214 ± 4.3
0.296GluCys: 0.296 ± 0.299
3.85GluAsp: 3.85 ± 0.92
8.291GluGlu: 8.291 ± 2.929
0.592GluPhe: 0.592 ± 0.389
4.146GluGly: 4.146 ± 1.356
0.592GluHis: 0.592 ± 0.384
2.369GluIle: 2.369 ± 1.072
2.369GluLys: 2.369 ± 0.827
5.33GluLeu: 5.33 ± 1.218
2.665GluMet: 2.665 ± 0.834
1.481GluAsn: 1.481 ± 0.702
2.665GluPro: 2.665 ± 0.997
2.073GluGln: 2.073 ± 0.625
3.85GluArg: 3.85 ± 1.268
0.888GluSer: 0.888 ± 0.45
2.073GluThr: 2.073 ± 1.074
8.884GluVal: 8.884 ± 2.182
3.257GluTrp: 3.257 ± 0.741
2.369GluTyr: 2.369 ± 0.911
0.0GluXaa: 0.0 ± 0.0
Phe
1.777PheAla: 1.777 ± 0.832
0.0PheCys: 0.0 ± 0.0
0.296PheAsp: 0.296 ± 0.287
0.592PheGlu: 0.592 ± 0.377
0.592PhePhe: 0.592 ± 0.381
0.888PheGly: 0.888 ± 0.437
0.592PheHis: 0.592 ± 0.409
1.184PheIle: 1.184 ± 0.761
0.0PheLys: 0.0 ± 0.0
2.961PheLeu: 2.961 ± 1.183
0.296PheMet: 0.296 ± 0.324
0.296PheAsn: 0.296 ± 0.281
1.481PhePro: 1.481 ± 0.671
1.777PheGln: 1.777 ± 0.802
2.073PheArg: 2.073 ± 0.761
0.888PheSer: 0.888 ± 0.401
1.184PheThr: 1.184 ± 0.51
1.481PheVal: 1.481 ± 0.47
2.073PheTrp: 2.073 ± 0.888
0.0PheTyr: 0.0 ± 0.0
0.0PheXaa: 0.0 ± 0.0
Gly
9.18GlyAla: 9.18 ± 1.847
0.0GlyCys: 0.0 ± 0.0
3.85GlyAsp: 3.85 ± 0.857
8.291GlyGlu: 8.291 ± 1.715
3.257GlyPhe: 3.257 ± 1.134
10.66GlyGly: 10.66 ± 1.994
1.184GlyHis: 1.184 ± 0.533
1.777GlyIle: 1.777 ± 0.517
4.146GlyLys: 4.146 ± 1.044
8.588GlyLeu: 8.588 ± 1.338
1.481GlyMet: 1.481 ± 0.575
2.073GlyAsn: 2.073 ± 0.78
4.442GlyPro: 4.442 ± 0.84
2.073GlyGln: 2.073 ± 0.83
7.699GlyArg: 7.699 ± 1.441
2.961GlySer: 2.961 ± 1.215
6.811GlyThr: 6.811 ± 1.411
5.626GlyVal: 5.626 ± 1.158
2.665GlyTrp: 2.665 ± 0.89
5.034GlyTyr: 5.034 ± 1.341
0.0GlyXaa: 0.0 ± 0.0
His
1.184HisAla: 1.184 ± 0.479
0.0HisCys: 0.0 ± 0.0
0.888HisAsp: 0.888 ± 0.58
0.888HisGlu: 0.888 ± 0.562
0.296HisPhe: 0.296 ± 0.272
0.888HisGly: 0.888 ± 0.539
0.592HisHis: 0.592 ± 0.409
0.296HisIle: 0.296 ± 0.281
0.592HisLys: 0.592 ± 0.389
1.184HisLeu: 1.184 ± 0.509
0.0HisMet: 0.0 ± 0.0
0.0HisAsn: 0.0 ± 0.0
2.073HisPro: 2.073 ± 0.773
0.592HisGln: 0.592 ± 0.426
0.888HisArg: 0.888 ± 0.459
0.296HisSer: 0.296 ± 0.263
1.184HisThr: 1.184 ± 0.537
1.184HisVal: 1.184 ± 0.523
1.184HisTrp: 1.184 ± 0.599
1.777HisTyr: 1.777 ± 0.753
0.0HisXaa: 0.0 ± 0.0
Ile
3.85IleAla: 3.85 ± 1.148
0.888IleCys: 0.888 ± 0.594
0.592IleAsp: 0.592 ± 0.403
0.888IleGlu: 0.888 ± 0.431
1.481IlePhe: 1.481 ± 0.586
5.034IleGly: 5.034 ± 1.501
1.184IleHis: 1.184 ± 0.695
1.777IleIle: 1.777 ± 0.626
0.888IleLys: 0.888 ± 0.6
5.626IleLeu: 5.626 ± 0.985
0.888IleMet: 0.888 ± 0.522
0.296IleAsn: 0.296 ± 0.281
3.553IlePro: 3.553 ± 1.291
1.777IleGln: 1.777 ± 0.68
3.553IleArg: 3.553 ± 1.037
1.184IleSer: 1.184 ± 0.498
2.073IleThr: 2.073 ± 0.751
2.665IleVal: 2.665 ± 1.051
0.592IleTrp: 0.592 ± 0.4
1.481IleTyr: 1.481 ± 0.776
0.0IleXaa: 0.0 ± 0.0
Lys
3.85LysAla: 3.85 ± 0.937
0.296LysCys: 0.296 ± 0.272
1.184LysAsp: 1.184 ± 0.491
1.481LysGlu: 1.481 ± 0.674
0.0LysPhe: 0.0 ± 0.0
2.073LysGly: 2.073 ± 0.746
0.592LysHis: 0.592 ± 0.365
1.481LysIle: 1.481 ± 0.613
0.592LysLys: 0.592 ± 0.366
3.85LysLeu: 3.85 ± 0.934
0.888LysMet: 0.888 ± 0.399
0.592LysAsn: 0.592 ± 0.378
1.481LysPro: 1.481 ± 0.647
0.888LysGln: 0.888 ± 0.648
2.961LysArg: 2.961 ± 0.888
1.777LysSer: 1.777 ± 0.684
1.481LysThr: 1.481 ± 0.594
2.369LysVal: 2.369 ± 0.785
0.592LysTrp: 0.592 ± 0.379
0.888LysTyr: 0.888 ± 0.451
0.0LysXaa: 0.0 ± 0.0
Leu
12.141LeuAla: 12.141 ± 1.459
0.296LeuCys: 0.296 ± 0.299
5.034LeuAsp: 5.034 ± 1.135
7.107LeuGlu: 7.107 ± 1.467
1.777LeuPhe: 1.777 ± 0.867
7.699LeuGly: 7.699 ± 1.649
2.073LeuHis: 2.073 ± 0.646
4.146LeuIle: 4.146 ± 0.827
4.442LeuLys: 4.442 ± 1.033
10.956LeuLeu: 10.956 ± 1.931
1.481LeuMet: 1.481 ± 0.732
4.738LeuAsn: 4.738 ± 1.24
9.476LeuPro: 9.476 ± 1.646
2.665LeuGln: 2.665 ± 1.085
8.588LeuArg: 8.588 ± 1.755
6.515LeuSer: 6.515 ± 1.389
4.146LeuThr: 4.146 ± 0.905
10.068LeuVal: 10.068 ± 1.932
1.184LeuTrp: 1.184 ± 0.664
2.665LeuTyr: 2.665 ± 0.953
0.0LeuXaa: 0.0 ± 0.0
Met
3.257MetAla: 3.257 ± 1.221
0.296MetCys: 0.296 ± 0.262
1.481MetAsp: 1.481 ± 0.592
2.073MetGlu: 2.073 ± 0.666
0.296MetPhe: 0.296 ± 0.324
2.369MetGly: 2.369 ± 0.752
0.296MetHis: 0.296 ± 0.298
1.184MetIle: 1.184 ± 0.619
0.296MetLys: 0.296 ± 0.288
0.888MetLeu: 0.888 ± 0.468
0.296MetMet: 0.296 ± 0.263
0.888MetAsn: 0.888 ± 0.432
1.777MetPro: 1.777 ± 0.587
0.0MetGln: 0.0 ± 0.0
1.184MetArg: 1.184 ± 0.582
1.481MetSer: 1.481 ± 0.713
0.592MetThr: 0.592 ± 0.435
0.592MetVal: 0.592 ± 0.42
0.888MetTrp: 0.888 ± 0.569
1.184MetTyr: 1.184 ± 0.48
0.0MetXaa: 0.0 ± 0.0
Asn
4.738AsnAla: 4.738 ± 1.587
0.0AsnCys: 0.0 ± 0.0
0.296AsnAsp: 0.296 ± 0.3
0.296AsnGlu: 0.296 ± 0.272
1.184AsnPhe: 1.184 ± 0.489
2.961AsnGly: 2.961 ± 1.035
0.592AsnHis: 0.592 ± 0.4
0.296AsnIle: 0.296 ± 0.281
0.296AsnLys: 0.296 ± 0.298
3.85AsnLeu: 3.85 ± 1.008
0.592AsnMet: 0.592 ± 0.443
1.481AsnAsn: 1.481 ± 0.689
1.184AsnPro: 1.184 ± 0.545
0.0AsnGln: 0.0 ± 0.0
2.073AsnArg: 2.073 ± 0.672
1.184AsnSer: 1.184 ± 0.735
1.184AsnThr: 1.184 ± 0.633
1.184AsnVal: 1.184 ± 0.816
0.296AsnTrp: 0.296 ± 0.272
0.592AsnTyr: 0.592 ± 0.389
0.0AsnXaa: 0.0 ± 0.0
Pro
3.257ProAla: 3.257 ± 1.112
0.0ProCys: 0.0 ± 0.0
1.777ProAsp: 1.777 ± 0.593
7.107ProGlu: 7.107 ± 1.563
1.481ProPhe: 1.481 ± 0.667
7.107ProGly: 7.107 ± 1.442
1.184ProHis: 1.184 ± 0.769
3.257ProIle: 3.257 ± 0.757
2.073ProLys: 2.073 ± 0.689
5.034ProLeu: 5.034 ± 1.249
1.184ProMet: 1.184 ± 0.516
1.481ProAsn: 1.481 ± 0.64
5.034ProPro: 5.034 ± 1.351
3.85ProGln: 3.85 ± 1.214
3.85ProArg: 3.85 ± 1.008
2.961ProSer: 2.961 ± 0.758
5.626ProThr: 5.626 ± 1.421
5.33ProVal: 5.33 ± 1.493
2.073ProTrp: 2.073 ± 0.586
2.665ProTyr: 2.665 ± 1.208
0.0ProXaa: 0.0 ± 0.0
Gln
4.442GlnAla: 4.442 ± 0.953
0.0GlnCys: 0.0 ± 0.0
1.777GlnAsp: 1.777 ± 0.61
2.369GlnGlu: 2.369 ± 0.672
0.296GlnPhe: 0.296 ± 0.321
4.442GlnGly: 4.442 ± 1.325
0.296GlnHis: 0.296 ± 0.324
1.481GlnIle: 1.481 ± 0.645
1.184GlnLys: 1.184 ± 0.712
2.369GlnLeu: 2.369 ± 0.869
0.888GlnMet: 0.888 ± 0.583
0.592GlnAsn: 0.592 ± 0.448
2.073GlnPro: 2.073 ± 0.7
0.888GlnGln: 0.888 ± 0.42
1.777GlnArg: 1.777 ± 0.621
0.888GlnSer: 0.888 ± 0.438
1.481GlnThr: 1.481 ± 0.674
2.369GlnVal: 2.369 ± 0.693
0.296GlnTrp: 0.296 ± 0.263
0.296GlnTyr: 0.296 ± 0.287
0.0GlnXaa: 0.0 ± 0.0
Arg
6.811ArgAla: 6.811 ± 1.208
0.0ArgCys: 0.0 ± 0.0
2.961ArgAsp: 2.961 ± 0.817
5.922ArgGlu: 5.922 ± 1.304
1.184ArgPhe: 1.184 ± 0.444
5.922ArgGly: 5.922 ± 1.019
1.184ArgHis: 1.184 ± 0.687
4.442ArgIle: 4.442 ± 0.826
3.257ArgLys: 3.257 ± 0.716
8.291ArgLeu: 8.291 ± 1.39
2.369ArgMet: 2.369 ± 0.752
1.481ArgAsn: 1.481 ± 0.701
4.738ArgPro: 4.738 ± 1.39
2.369ArgGln: 2.369 ± 0.676
6.811ArgArg: 6.811 ± 1.969
2.073ArgSer: 2.073 ± 0.712
1.777ArgThr: 1.777 ± 0.699
5.922ArgVal: 5.922 ± 1.217
3.85ArgTrp: 3.85 ± 1.047
2.665ArgTyr: 2.665 ± 0.879
0.0ArgXaa: 0.0 ± 0.0
Ser
5.626SerAla: 5.626 ± 1.682
0.0SerCys: 0.0 ± 0.0
2.073SerAsp: 2.073 ± 0.502
1.777SerGlu: 1.777 ± 0.729
0.0SerPhe: 0.0 ± 0.0
6.515SerGly: 6.515 ± 1.574
0.0SerHis: 0.0 ± 0.0
1.184SerIle: 1.184 ± 0.773
0.592SerLys: 0.592 ± 0.378
3.85SerLeu: 3.85 ± 1.084
1.481SerMet: 1.481 ± 0.708
1.481SerAsn: 1.481 ± 0.654
2.961SerPro: 2.961 ± 0.685
0.296SerGln: 0.296 ± 0.281
3.553SerArg: 3.553 ± 0.985
1.184SerSer: 1.184 ± 1.017
3.257SerThr: 3.257 ± 1.247
3.553SerVal: 3.553 ± 1.241
0.592SerTrp: 0.592 ± 0.397
0.888SerTyr: 0.888 ± 0.51
0.0SerXaa: 0.0 ± 0.0
Thr
3.553ThrAla: 3.553 ± 1.117
0.0ThrCys: 0.0 ± 0.0
2.369ThrAsp: 2.369 ± 0.899
2.073ThrGlu: 2.073 ± 0.833
1.777ThrPhe: 1.777 ± 0.981
5.33ThrGly: 5.33 ± 1.379
0.888ThrHis: 0.888 ± 0.462
3.553ThrIle: 3.553 ± 0.908
0.592ThrLys: 0.592 ± 0.365
5.33ThrLeu: 5.33 ± 1.154
0.296ThrMet: 0.296 ± 0.288
0.888ThrAsn: 0.888 ± 0.612
5.034ThrPro: 5.034 ± 1.445
2.665ThrGln: 2.665 ± 1.032
2.369ThrArg: 2.369 ± 0.668
2.369ThrSer: 2.369 ± 1.022
2.961ThrThr: 2.961 ± 1.114
5.33ThrVal: 5.33 ± 1.03
2.665ThrTrp: 2.665 ± 0.823
2.073ThrTyr: 2.073 ± 0.779
0.0ThrXaa: 0.0 ± 0.0
Val
8.291ValAla: 8.291 ± 1.555
0.296ValCys: 0.296 ± 0.296
4.146ValAsp: 4.146 ± 0.901
7.403ValGlu: 7.403 ± 2.254
0.888ValPhe: 0.888 ± 0.438
8.884ValGly: 8.884 ± 1.465
0.888ValHis: 0.888 ± 0.42
2.961ValIle: 2.961 ± 0.897
2.073ValLys: 2.073 ± 0.799
9.476ValLeu: 9.476 ± 2.048
2.073ValMet: 2.073 ± 0.566
1.777ValAsn: 1.777 ± 0.755
4.738ValPro: 4.738 ± 1.23
2.369ValGln: 2.369 ± 1.105
6.811ValArg: 6.811 ± 1.671
3.85ValSer: 3.85 ± 1.035
2.961ValThr: 2.961 ± 1.16
10.66ValVal: 10.66 ± 2.094
2.665ValTrp: 2.665 ± 1.011
5.034ValTyr: 5.034 ± 1.117
0.0ValXaa: 0.0 ± 0.0
Trp
3.553TrpAla: 3.553 ± 0.855
0.0TrpCys: 0.0 ± 0.0
1.481TrpAsp: 1.481 ± 0.508
3.85TrpGlu: 3.85 ± 0.89
0.296TrpPhe: 0.296 ± 0.263
2.665TrpGly: 2.665 ± 0.977
0.296TrpHis: 0.296 ± 0.281
0.888TrpIle: 0.888 ± 0.476
0.888TrpLys: 0.888 ± 0.483
3.257TrpLeu: 3.257 ± 1.099
0.0TrpMet: 0.0 ± 0.0
0.888TrpAsn: 0.888 ± 0.444
3.257TrpPro: 3.257 ± 1.224
0.0TrpGln: 0.0 ± 0.0
3.85TrpArg: 3.85 ± 1.286
1.777TrpSer: 1.777 ± 0.754
1.481TrpThr: 1.481 ± 0.711
1.481TrpVal: 1.481 ± 0.718
0.888TrpTrp: 0.888 ± 0.454
1.481TrpTyr: 1.481 ± 0.73
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.85TyrAla: 3.85 ± 0.728
0.0TyrCys: 0.0 ± 0.0
2.073TyrAsp: 2.073 ± 0.728
3.257TyrGlu: 3.257 ± 0.769
1.184TyrPhe: 1.184 ± 0.504
3.85TyrGly: 3.85 ± 0.822
1.777TyrHis: 1.777 ± 0.856
1.481TyrIle: 1.481 ± 0.562
0.592TyrLys: 0.592 ± 0.368
6.219TyrLeu: 6.219 ± 1.198
0.592TyrMet: 0.592 ± 0.421
1.184TyrAsn: 1.184 ± 0.51
3.257TyrPro: 3.257 ± 0.95
1.481TyrGln: 1.481 ± 0.581
2.073TyrArg: 2.073 ± 0.811
1.184TyrSer: 1.184 ± 0.574
2.073TyrThr: 2.073 ± 0.791
3.553TyrVal: 3.553 ± 0.888
0.888TyrTrp: 0.888 ± 0.624
1.481TyrTyr: 1.481 ± 0.688
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 26 proteins (3378 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski