Streptomyces sp. RKND-216

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4607 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4S2RFW5|A0A4S2RFW5_9ACTN Foldase YidC OS=Streptomyces sp. RKND-216 OX=2562581 GN=yidC PE=3 SV=1
MM1 pKa = 7.41AAAGALIATALTGPAAGAAPAEE23 pKa = 4.6DD24 pKa = 4.29DD25 pKa = 3.96GSRR28 pKa = 11.84AAAQLLDD35 pKa = 3.62NAGFEE40 pKa = 4.46AGLEE44 pKa = 4.18GWTCSGDD51 pKa = 3.53SGTAAASPARR61 pKa = 11.84SGVRR65 pKa = 11.84ALTATPAGQDD75 pKa = 3.22NARR78 pKa = 11.84CSQVVAVEE86 pKa = 3.99PDD88 pKa = 3.01ADD90 pKa = 3.91YY91 pKa = 10.8TLSGWVQGSYY101 pKa = 10.95VYY103 pKa = 10.78LGATGTGTTDD113 pKa = 2.66VATWTGSTPSWKK125 pKa = 9.81QLSTTFRR132 pKa = 11.84TGPDD136 pKa = 3.27TTSVTVYY143 pKa = 9.75LHH145 pKa = 5.75GWYY148 pKa = 10.23GQGAYY153 pKa = 10.02HH154 pKa = 7.38ADD156 pKa = 3.79DD157 pKa = 4.84LALSGPGEE165 pKa = 4.48PGDD168 pKa = 4.55PGDD171 pKa = 4.49PGDD174 pKa = 4.36PEE176 pKa = 4.48VPAVPSGLAVGAVTAGSVGLSWTEE200 pKa = 3.9VPDD203 pKa = 3.24ATAYY207 pKa = 9.79HH208 pKa = 6.99VYY210 pKa = 10.55RR211 pKa = 11.84DD212 pKa = 3.86GTKK215 pKa = 9.43VASAEE220 pKa = 4.24GASATVTGLSPSTSYY235 pKa = 11.42AFRR238 pKa = 11.84VSAVNGAGEE247 pKa = 4.37SAKK250 pKa = 10.54SAAVTARR257 pKa = 11.84TSADD261 pKa = 3.28GGDD264 pKa = 3.88GGTTVPEE271 pKa = 4.27HH272 pKa = 6.69AVTGYY277 pKa = 9.64VQNFDD282 pKa = 4.67NGAQTQRR289 pKa = 11.84LTEE292 pKa = 3.94VQDD295 pKa = 3.87AYY297 pKa = 11.15DD298 pKa = 4.22IIAVAFASSTSTPGEE313 pKa = 3.93LTFALDD319 pKa = 3.47PALAPSDD326 pKa = 4.08DD327 pKa = 3.91AFKK330 pKa = 11.29ADD332 pKa = 3.33IAAKK336 pKa = 9.28QAEE339 pKa = 4.73GKK341 pKa = 10.38AVILSVGGEE350 pKa = 4.12LGNVTVNDD358 pKa = 4.17DD359 pKa = 3.03ASARR363 pKa = 11.84AFADD367 pKa = 3.69SAWALMQEE375 pKa = 4.24YY376 pKa = 9.84GFDD379 pKa = 4.43GIDD382 pKa = 3.02IDD384 pKa = 4.98LEE386 pKa = 4.23HH387 pKa = 7.34GINSTYY393 pKa = 9.25LTRR396 pKa = 11.84AIRR399 pKa = 11.84DD400 pKa = 3.39LAANAGPDD408 pKa = 3.91FVLTMAPQTLDD419 pKa = 3.33MQSTSTEE426 pKa = 4.27YY427 pKa = 9.12FTLALNVKK435 pKa = 10.3DD436 pKa = 3.51ILTVVNMQYY445 pKa = 11.07YY446 pKa = 10.53NSGSMLGCDD455 pKa = 3.33GQVYY459 pKa = 10.08SQGSVDD465 pKa = 5.27FLTALACIQLRR476 pKa = 11.84GGLDD480 pKa = 3.27ASQVGLGTPASPRR493 pKa = 11.84GAGSGYY499 pKa = 10.36VDD501 pKa = 3.29PSVVNDD507 pKa = 4.17ALSCLTRR514 pKa = 11.84GTNCGSFQPSQTYY527 pKa = 9.41PSLRR531 pKa = 11.84GAMTWSTNWDD541 pKa = 3.96ALNGDD546 pKa = 3.36QWSNAVGPHH555 pKa = 4.7VHH557 pKa = 6.75GLPP560 pKa = 3.71

Molecular weight: 56.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4S2R4B0|A0A4S2R4B0_9ACTN CDP-alcohol phosphatidyltransferase family protein OS=Streptomyces sp. RKND-216 OX=2562581 GN=E4198_00700 PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AIVANRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.81GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4607

0

4607

1585695

25

8058

344.2

36.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.803 ± 0.065

0.827 ± 0.01

6.214 ± 0.034

6.009 ± 0.035

2.666 ± 0.02

9.862 ± 0.046

2.418 ± 0.021

2.663 ± 0.025

1.843 ± 0.027

10.287 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.706 ± 0.016

1.57 ± 0.016

6.281 ± 0.034

2.638 ± 0.021

8.53 ± 0.046

4.819 ± 0.026

5.93 ± 0.039

8.539 ± 0.037

1.441 ± 0.02

1.953 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski