Nocardioides sp. zg-579

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Nocardioidaceae; Nocardioides; unclassified Nocardioides

Average proteome isoelectric point is 5.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4040 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6I3JA02|A0A6I3JA02_9ACTN SDR family NAD(P)-dependent oxidoreductase OS=Nocardioides sp. zg-579 OX=2663857 GN=GGQ22_06345 PE=3 SV=1
MM1 pKa = 7.78LKK3 pKa = 10.36RR4 pKa = 11.84PLAALATLAAVSLLASCGDD23 pKa = 4.26DD24 pKa = 3.72EE25 pKa = 5.31SSPDD29 pKa = 4.03AGDD32 pKa = 3.7SPAASASSGSEE43 pKa = 3.53KK44 pKa = 9.97ATPSAEE50 pKa = 4.15GASGPTCVYY59 pKa = 10.0PQDD62 pKa = 4.01GQPAAKK68 pKa = 10.09DD69 pKa = 3.5VEE71 pKa = 4.71LPPNPAAVKK80 pKa = 8.7GTIEE84 pKa = 4.16GSMATTIGDD93 pKa = 3.27IGISLDD99 pKa = 3.87AKK101 pKa = 10.82ASPCTVNSFVSLAEE115 pKa = 3.54QGYY118 pKa = 10.28FDD120 pKa = 5.23DD121 pKa = 4.65TTCHH125 pKa = 7.07RR126 pKa = 11.84LTTADD131 pKa = 3.04TGIEE135 pKa = 4.01VLQCGDD141 pKa = 3.44PTGTGTGGPGYY152 pKa = 9.07TIEE155 pKa = 5.9DD156 pKa = 4.14EE157 pKa = 4.47VTPDD161 pKa = 3.02TTYY164 pKa = 10.69PAGTLAMAKK173 pKa = 9.07TPAPDD178 pKa = 3.13SGGSQFFIVYY188 pKa = 10.01GDD190 pKa = 3.65TPLPPEE196 pKa = 3.92YY197 pKa = 9.91TVFGSIDD204 pKa = 3.57EE205 pKa = 4.99AGLKK209 pKa = 9.81AVQEE213 pKa = 4.2AAAEE217 pKa = 4.36GTTDD221 pKa = 4.4GGPDD225 pKa = 3.89GQPKK229 pKa = 9.47VAVDD233 pKa = 3.75IEE235 pKa = 4.75SVTIDD240 pKa = 3.68DD241 pKa = 4.0

Molecular weight: 24.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6I3J0J2|A0A6I3J0J2_9ACTN Uncharacterized protein OS=Nocardioides sp. zg-579 OX=2663857 GN=GGQ22_07900 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.99KK16 pKa = 9.77HH17 pKa = 5.81RR18 pKa = 11.84KK19 pKa = 8.51LLKK22 pKa = 8.15KK23 pKa = 9.24TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84KK30 pKa = 10.07LGKK33 pKa = 9.87

Molecular weight: 4.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4040

0

4040

1327210

29

2470

328.5

35.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.693 ± 0.065

0.724 ± 0.01

6.341 ± 0.035

5.989 ± 0.04

2.607 ± 0.019

9.44 ± 0.035

2.188 ± 0.02

3.002 ± 0.025

1.754 ± 0.031

10.388 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.731 ± 0.016

1.546 ± 0.019

5.906 ± 0.03

2.605 ± 0.02

8.028 ± 0.04

4.89 ± 0.023

6.001 ± 0.032

9.79 ± 0.037

1.52 ± 0.017

1.856 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski