Streptomyces carminius

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5703 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2M8LSQ1|A0A2M8LSQ1_9ACTN RimJ/RimL family protein N-acetyltransferase OS=Streptomyces carminius OX=2665496 GN=CUT44_25280 PE=4 SV=1
MM1 pKa = 7.55GAQNEE6 pKa = 4.5QVTGLEE12 pKa = 3.79VWIDD16 pKa = 3.58QEE18 pKa = 4.65LCTGDD23 pKa = 4.86GICAQYY29 pKa = 11.17APDD32 pKa = 4.08VFEE35 pKa = 6.18LDD37 pKa = 3.22IDD39 pKa = 3.58GLAYY43 pKa = 10.51VKK45 pKa = 10.76APAPAGEE52 pKa = 4.12EE53 pKa = 4.37AEE55 pKa = 4.95LLTAPGASAPVPLPLLNDD73 pKa = 3.66VVDD76 pKa = 4.32SARR79 pKa = 11.84EE80 pKa = 3.94CPGEE84 pKa = 4.38CIHH87 pKa = 6.3VRR89 pKa = 11.84RR90 pKa = 11.84VSDD93 pKa = 3.29GTEE96 pKa = 3.6VHH98 pKa = 6.66GPDD101 pKa = 3.32AAEE104 pKa = 4.51APGTGNTPP112 pKa = 3.1

Molecular weight: 11.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2M8MBX3|A0A2M8MBX3_9ACTN TetR family transcriptional regulator OS=Streptomyces carminius OX=2665496 GN=CUT44_00900 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AIVAARR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.28GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5703

0

5703

1860990

30

7517

326.3

34.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.802 ± 0.052

0.822 ± 0.01

5.861 ± 0.025

6.181 ± 0.032

2.536 ± 0.018

10.081 ± 0.028

2.303 ± 0.016

2.686 ± 0.02

1.746 ± 0.02

10.262 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.594 ± 0.013

1.603 ± 0.015

6.503 ± 0.032

2.421 ± 0.021

9.056 ± 0.039

4.646 ± 0.026

6.032 ± 0.033

8.396 ± 0.028

1.486 ± 0.014

1.982 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski