Paenibacillus sp. CAA11
Average proteome isoelectric point is 6.34
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4316 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2S0UDJ0|A0A2S0UDJ0_9BACL Signal peptidase I OS=Paenibacillus sp. CAA11 OX=1532905 GN=DCC85_17810 PE=4 SV=1
MM1 pKa = 7.57 RR2 pKa = 11.84 PIIEE6 pKa = 4.29 FCTNNMHH13 pKa = 7.32 FGTDD17 pKa = 3.01 EE18 pKa = 3.97 VMEE21 pKa = 4.44 KK22 pKa = 10.91 LEE24 pKa = 4.47 EE25 pKa = 4.28 NPDD28 pKa = 3.76 YY29 pKa = 11.31 DD30 pKa = 3.83 VLEE33 pKa = 4.27 YY34 pKa = 11.31 GCLNNCGQCFMMPYY48 pKa = 10.48 ALVNGEE54 pKa = 3.93 IVEE57 pKa = 4.44 ADD59 pKa = 3.86 SADD62 pKa = 3.55 QLYY65 pKa = 11.09 DD66 pKa = 4.58 RR67 pKa = 11.84 ILAKK71 pKa = 10.29 IKK73 pKa = 10.31 EE74 pKa = 4.17 LEE76 pKa = 3.97 AWDD79 pKa = 5.39 ALDD82 pKa = 5.31 LDD84 pKa = 4.34
Molecular weight: 9.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.789
IPC2_protein 3.872
IPC_protein 3.795
Toseland 3.605
ProMoST 3.884
Dawson 3.783
Bjellqvist 3.986
Wikipedia 3.694
Rodwell 3.63
Grimsley 3.528
Solomon 3.757
Lehninger 3.719
Nozaki 3.91
DTASelect 4.075
Thurlkill 3.656
EMBOSS 3.706
Sillero 3.923
Patrickios 0.54
IPC_peptide 3.757
IPC2_peptide 3.897
IPC2.peptide.svr19 3.867
Protein with the highest isoelectric point:
>tr|A0A2S0UFY9|A0A2S0UFY9_9BACL Polysacc_synt_C domain-containing protein OS=Paenibacillus sp. CAA11 OX=1532905 GN=DCC85_00225 PE=4 SV=1
MM1 pKa = 7.9 RR2 pKa = 11.84 PTFKK6 pKa = 10.82 PNVSKK11 pKa = 10.8 RR12 pKa = 11.84 KK13 pKa = 8.95 KK14 pKa = 8.25 VHH16 pKa = 5.49 GFRR19 pKa = 11.84 KK20 pKa = 10.01 RR21 pKa = 11.84 MSTKK25 pKa = 10.18 NGRR28 pKa = 11.84 KK29 pKa = 8.87 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 LKK37 pKa = 10.2 GRR39 pKa = 11.84 KK40 pKa = 8.79 VLSAA44 pKa = 4.05
Molecular weight: 5.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.494
IPC2_protein 11.125
IPC_protein 12.691
Toseland 12.866
ProMoST 13.349
Dawson 12.866
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.705
Grimsley 12.91
Solomon 13.349
Lehninger 13.247
Nozaki 12.866
DTASelect 12.852
Thurlkill 12.866
EMBOSS 13.364
Sillero 12.866
Patrickios 12.427
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.077
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4316
0
4316
1369789
25
2524
317.4
35.37
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.199 ± 0.044
0.759 ± 0.013
5.012 ± 0.028
7.049 ± 0.047
3.948 ± 0.03
7.493 ± 0.035
2.11 ± 0.019
6.582 ± 0.036
5.521 ± 0.036
10.409 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.745 ± 0.019
3.623 ± 0.029
4.003 ± 0.026
4.065 ± 0.028
5.157 ± 0.033
6.426 ± 0.034
5.216 ± 0.034
6.956 ± 0.032
1.202 ± 0.017
3.526 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here