Streptococcus phage Javan372

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6B2Y4|A0A4D6B2Y4_9CAUD Uncharacterized protein OS=Streptococcus phage Javan372 OX=2548133 GN=Javan372_0010 PE=4 SV=1
MM1 pKa = 7.34ATLNDD6 pKa = 3.44ILNYY10 pKa = 10.38AEE12 pKa = 4.66SLANQGVGADD22 pKa = 3.44ADD24 pKa = 4.0GAYY27 pKa = 7.91GTQCVDD33 pKa = 3.75LPNSISINFFGKK45 pKa = 10.41ALWGNAIDD53 pKa = 4.98LLNSAAGLGYY63 pKa = 10.37EE64 pKa = 4.27VVYY67 pKa = 10.7DD68 pKa = 3.72AVGVNPRR75 pKa = 11.84AGAIFVMDD83 pKa = 3.66TTYY86 pKa = 11.16LYY88 pKa = 10.35GHH90 pKa = 7.66PYY92 pKa = 8.61GHH94 pKa = 6.69TGIVIEE100 pKa = 5.13DD101 pKa = 3.6SDD103 pKa = 4.53GVTMKK108 pKa = 10.39TIEE111 pKa = 4.01QNIDD115 pKa = 2.88GNADD119 pKa = 3.3SLYY122 pKa = 9.74VGGPARR128 pKa = 11.84YY129 pKa = 7.46NTRR132 pKa = 11.84NFDD135 pKa = 4.25GIVGWFYY142 pKa = 11.2FPTDD146 pKa = 3.51DD147 pKa = 3.53TSVAFEE153 pKa = 4.01QPEE156 pKa = 3.95PSEE159 pKa = 4.02PLTIEE164 pKa = 4.11SNGFHH169 pKa = 7.41PEE171 pKa = 3.68TGTFTVEE178 pKa = 3.56VSALNVRR185 pKa = 11.84VEE187 pKa = 4.14AGLGAEE193 pKa = 3.97IVAVYY198 pKa = 10.41SAGQEE203 pKa = 3.68INYY206 pKa = 10.02DD207 pKa = 3.06GWIDD211 pKa = 3.46NDD213 pKa = 4.08GYY215 pKa = 10.9IWISYY220 pKa = 9.81IGGSGNRR227 pKa = 11.84RR228 pKa = 11.84YY229 pKa = 10.25VAVGKK234 pKa = 10.16SEE236 pKa = 3.71NGQRR240 pKa = 11.84ITDD243 pKa = 4.32FGSFKK248 pKa = 11.08

Molecular weight: 26.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6B562|A0A4D6B562_9CAUD Uncharacterized protein OS=Streptococcus phage Javan372 OX=2548133 GN=Javan372_0032 PE=4 SV=1
MM1 pKa = 6.31TTIEE5 pKa = 4.9IILAVAFVIYY15 pKa = 10.38ILLSGFAIYY24 pKa = 10.89VMRR27 pKa = 11.84EE28 pKa = 3.66VIVRR32 pKa = 11.84QKK34 pKa = 11.6AKK36 pKa = 8.93MKK38 pKa = 9.62HH39 pKa = 5.1YY40 pKa = 10.68KK41 pKa = 8.22SAKK44 pKa = 6.91YY45 pKa = 9.04QRR47 pKa = 11.84EE48 pKa = 3.87MWNKK52 pKa = 9.34RR53 pKa = 11.84MSEE56 pKa = 3.68IHH58 pKa = 5.82QKK60 pKa = 9.09RR61 pKa = 11.84TVKK64 pKa = 10.46GMSEE68 pKa = 3.95LL69 pKa = 3.76

Molecular weight: 8.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

9710

47

1134

176.5

20.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.632 ± 0.4

0.525 ± 0.112

6.004 ± 0.33

8.754 ± 0.42

4.645 ± 0.292

6.2 ± 0.367

1.421 ± 0.163

7.415 ± 0.341

8.97 ± 0.353

8.218 ± 0.27

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.667 ± 0.241

6.045 ± 0.233

2.482 ± 0.176

4.325 ± 0.215

3.666 ± 0.28

5.86 ± 0.26

5.664 ± 0.346

6.148 ± 0.271

1.215 ± 0.128

3.141 ± 0.281
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski