Flavobacterium humi

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Flavobacterium

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3054 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Z0L6N4|A0A4Z0L6N4_9FLAO Uncharacterized protein OS=Flavobacterium humi OX=2562683 GN=E4635_15100 PE=4 SV=1
MM1 pKa = 7.25NKK3 pKa = 9.97KK4 pKa = 10.33LLFLFIFFFLGLASVVSQNVSLYY27 pKa = 9.23SQFNGRR33 pKa = 11.84YY34 pKa = 9.07DD35 pKa = 3.35FAFIGNTLNTNEE47 pKa = 4.63NGTGAPCTITTSSSATVNLNPSDD70 pKa = 3.96MVEE73 pKa = 3.57AAYY76 pKa = 10.1LYY78 pKa = 9.41WAGSGTGDD86 pKa = 4.7FDD88 pKa = 4.65VKK90 pKa = 11.13LNDD93 pKa = 3.41VAITATRR100 pKa = 11.84QFSVIQPTSQRR111 pKa = 11.84PFFSAFADD119 pKa = 3.04ITTQVQTSGNGNYY132 pKa = 8.8TLSEE136 pKa = 4.25LDD138 pKa = 3.62LTNVIAQYY146 pKa = 10.65CPNGTNFGGWAIIIVYY162 pKa = 10.08KK163 pKa = 10.78NAALPLNQLNIYY175 pKa = 10.35DD176 pKa = 4.24GLQFVPDD183 pKa = 5.54AINITLDD190 pKa = 3.4SLNVIDD196 pKa = 6.22NEE198 pKa = 4.08DD199 pKa = 3.32AKK201 pKa = 10.86IGFVAWEE208 pKa = 4.23GDD210 pKa = 3.1RR211 pKa = 11.84SIAEE215 pKa = 4.1NEE217 pKa = 4.08SLRR220 pKa = 11.84INGNLISNPPLNPSDD235 pKa = 3.48NAFNGTNSFTGSSVLYY251 pKa = 11.22NMDD254 pKa = 4.0LDD256 pKa = 4.39VYY258 pKa = 10.73NIQNNIQIGDD268 pKa = 3.76EE269 pKa = 4.15SASIQLTSDD278 pKa = 3.18RR279 pKa = 11.84DD280 pKa = 3.64FVMINAIVTKK290 pKa = 10.5LNSQLPDD297 pKa = 2.95ATVAIEE303 pKa = 5.66NIAQEE308 pKa = 4.53CNTRR312 pKa = 11.84RR313 pKa = 11.84LTIQYY318 pKa = 10.17SIRR321 pKa = 11.84NFNATDD327 pKa = 4.11FLPSGTFTTIYY338 pKa = 10.69ADD340 pKa = 4.94GIAVGVVQTTEE351 pKa = 4.88DD352 pKa = 3.35IPIGEE357 pKa = 4.23TRR359 pKa = 11.84NYY361 pKa = 6.72EE362 pKa = 4.01TTVIIPASVAPVFDD376 pKa = 3.78LQFIVDD382 pKa = 3.96NEE384 pKa = 4.17NGVAHH389 pKa = 4.88VTEE392 pKa = 4.51TNEE395 pKa = 3.87NNNTFTQEE403 pKa = 3.64VALWLSPEE411 pKa = 3.67INAPQNVTACNEE423 pKa = 3.86GNTRR427 pKa = 11.84GTFDD431 pKa = 4.92FSLQTGAIPINPGDD445 pKa = 3.39TVLFFEE451 pKa = 4.74NNQDD455 pKa = 3.47AVLSQNPIINTTNYY469 pKa = 9.74LAQATPKK476 pKa = 9.12TIYY479 pKa = 10.38VRR481 pKa = 11.84IEE483 pKa = 3.75NEE485 pKa = 3.64HH486 pKa = 6.84CFTITHH492 pKa = 6.61FEE494 pKa = 4.13LVAIPCPPTVYY505 pKa = 10.83NYY507 pKa = 7.52FTPNGDD513 pKa = 3.62GLNDD517 pKa = 3.59SFFVEE522 pKa = 4.58GLRR525 pKa = 11.84DD526 pKa = 3.64VFLHH530 pKa = 6.54FEE532 pKa = 3.91LSIYY536 pKa = 9.76NRR538 pKa = 11.84WGSLVWEE545 pKa = 4.84GNNNTEE551 pKa = 4.23DD552 pKa = 3.39WDD554 pKa = 4.21GKK556 pKa = 10.39SNQPAILFHH565 pKa = 6.79GNLPEE570 pKa = 3.96GTYY573 pKa = 10.27FYY575 pKa = 11.43VLYY578 pKa = 10.17LNEE581 pKa = 4.78PGYY584 pKa = 9.89PSPLTGWVFMNRR596 pKa = 3.4

Molecular weight: 65.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Z0L5Y1|A0A4Z0L5Y1_9FLAO Phage tail protein OS=Flavobacterium humi OX=2562683 GN=E4635_11020 PE=4 SV=1
MM1 pKa = 7.54SVRR4 pKa = 11.84KK5 pKa = 9.68LKK7 pKa = 10.5PITPGQRR14 pKa = 11.84FRR16 pKa = 11.84VVNGFDD22 pKa = 5.49AITTDD27 pKa = 3.09KK28 pKa = 11.02PEE30 pKa = 4.34RR31 pKa = 11.84SLIAPIKK38 pKa = 10.47NSGGRR43 pKa = 11.84NSQGKK48 pKa = 6.33MTMRR52 pKa = 11.84YY53 pKa = 7.39TGGGHH58 pKa = 4.86KK59 pKa = 9.44QRR61 pKa = 11.84YY62 pKa = 8.74RR63 pKa = 11.84IIDD66 pKa = 3.85FKK68 pKa = 10.24RR69 pKa = 11.84TKK71 pKa = 10.49AGIPATVKK79 pKa = 10.65SIEE82 pKa = 4.0YY83 pKa = 10.15DD84 pKa = 3.43PNRR87 pKa = 11.84TAFIALLAYY96 pKa = 10.31ADD98 pKa = 3.96GEE100 pKa = 4.24KK101 pKa = 10.14TYY103 pKa = 10.54IIAQNGLQVGQKK115 pKa = 9.1VVSGEE120 pKa = 3.88GSAPEE125 pKa = 4.11IGNTLPLSKK134 pKa = 10.28IPLGTVISCIEE145 pKa = 3.9LRR147 pKa = 11.84PGQGAVIARR156 pKa = 11.84SAGTFAQLMARR167 pKa = 11.84DD168 pKa = 4.06GKK170 pKa = 10.75YY171 pKa = 9.05ATIKK175 pKa = 9.6MPSGEE180 pKa = 4.18TRR182 pKa = 11.84LILLTCSATIGAVSNSDD199 pKa = 2.96HH200 pKa = 6.02QLIVSGKK207 pKa = 9.19AGRR210 pKa = 11.84TRR212 pKa = 11.84WLGRR216 pKa = 11.84RR217 pKa = 11.84PRR219 pKa = 11.84TRR221 pKa = 11.84PVAMNPVDD229 pKa = 3.63HH230 pKa = 7.13PMGGGEE236 pKa = 4.08GRR238 pKa = 11.84SSGGHH243 pKa = 4.81PRR245 pKa = 11.84SRR247 pKa = 11.84KK248 pKa = 8.85GLPAKK253 pKa = 10.34GYY255 pKa = 7.39RR256 pKa = 11.84TRR258 pKa = 11.84SKK260 pKa = 10.74QNPSNKK266 pKa = 10.03YY267 pKa = 8.27IVEE270 pKa = 4.07RR271 pKa = 11.84RR272 pKa = 11.84KK273 pKa = 10.24KK274 pKa = 10.1

Molecular weight: 29.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3054

0

3054

1069261

33

4662

350.1

39.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.887 ± 0.05

0.843 ± 0.019

5.265 ± 0.033

6.314 ± 0.058

5.284 ± 0.04

6.503 ± 0.049

1.79 ± 0.025

7.658 ± 0.038

7.656 ± 0.069

9.001 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.211 ± 0.026

6.141 ± 0.05

3.589 ± 0.031

3.586 ± 0.027

3.227 ± 0.03

6.497 ± 0.04

6.199 ± 0.078

6.26 ± 0.038

1.008 ± 0.014

4.083 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski