Lumpfish ranavirus

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Megaviricetes; Pimascovirales; Iridoviridae; Alphairidovirinae; Ranavirus; European North Atlantic ranavirus

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 97 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3Q9T8Q8|A0A3Q9T8Q8_9VIRU NTPase OS=Lumpfish ranavirus OX=2501771 GN=ORF8 PE=4 SV=1
MM1 pKa = 7.83ANTQNSVMDD10 pKa = 4.01FVDD13 pKa = 3.5TNVIEE18 pKa = 4.34VVRR21 pKa = 11.84DD22 pKa = 3.31QGGLYY27 pKa = 10.28HH28 pKa = 7.1VILEE32 pKa = 4.57KK33 pKa = 10.36ATEE36 pKa = 3.83LAIPDD41 pKa = 3.93EE42 pKa = 4.81YY43 pKa = 10.73ILEE46 pKa = 4.37DD47 pKa = 4.89FYY49 pKa = 11.82SDD51 pKa = 3.69TYY53 pKa = 9.96KK54 pKa = 10.6HH55 pKa = 5.21QVMVLEE61 pKa = 5.29KK62 pKa = 10.22IPKK65 pKa = 8.94VWADD69 pKa = 3.0DD70 pKa = 3.16WGYY73 pKa = 11.33DD74 pKa = 3.65EE75 pKa = 6.0NVLEE79 pKa = 4.37EE80 pKa = 4.16

Molecular weight: 9.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3Q9T7F8|A0A3Q9T7F8_9VIRU Uncharacterized protein OS=Lumpfish ranavirus OX=2501771 GN=ORF36 PE=4 SV=1
MM1 pKa = 7.64LLHH4 pKa = 7.26LILNLKK10 pKa = 10.3SFLPKK15 pKa = 9.98RR16 pKa = 11.84NLAFRR21 pKa = 11.84YY22 pKa = 10.25DD23 pKa = 3.59IVALSLTHH31 pKa = 7.37KK32 pKa = 10.31ILLKK36 pKa = 10.31HH37 pKa = 5.64QPPEE41 pKa = 3.88NVFAALYY48 pKa = 9.96IDD50 pKa = 4.0SLHH53 pKa = 6.61GGRR56 pKa = 11.84HH57 pKa = 4.65QIMPGFGKK65 pKa = 10.51LSCPTLDD72 pKa = 3.31VGPGLGRR79 pKa = 11.84FAASHH84 pKa = 5.59FSEE87 pKa = 5.14RR88 pKa = 11.84VSQDD92 pKa = 2.44RR93 pKa = 11.84QAQGRR98 pKa = 11.84EE99 pKa = 3.81RR100 pKa = 11.84RR101 pKa = 11.84SVLLSQEE108 pKa = 3.96RR109 pKa = 11.84RR110 pKa = 11.84GSSGRR115 pKa = 11.84QPLYY119 pKa = 10.27SLLPHH124 pKa = 6.15RR125 pKa = 11.84PKK127 pKa = 10.66RR128 pKa = 11.84EE129 pKa = 3.88GVIGAALCYY138 pKa = 9.71TSASHH143 pKa = 6.32SLSPAALLPPVGRR156 pKa = 11.84GKK158 pKa = 10.22DD159 pKa = 3.36RR160 pKa = 11.84HH161 pKa = 6.76CGRR164 pKa = 11.84QVGAAGLLRR173 pKa = 11.84HH174 pKa = 5.84VGRR177 pKa = 11.84RR178 pKa = 11.84RR179 pKa = 11.84QFAQLTRR186 pKa = 11.84GPLGLPCALLEE197 pKa = 4.35PPPLDD202 pKa = 3.32PHH204 pKa = 7.83LLDD207 pKa = 3.87KK208 pKa = 11.36GRR210 pKa = 11.84DD211 pKa = 3.52VGVGGCHH218 pKa = 6.38DD219 pKa = 3.93HH220 pKa = 7.14QGQEE224 pKa = 4.61DD225 pKa = 4.34GLLLHH230 pKa = 6.99GGPEE234 pKa = 4.27PVLGPQGVIGPAYY247 pKa = 9.79GRR249 pKa = 11.84PHH251 pKa = 7.16SNVHH255 pKa = 5.19ALGYY259 pKa = 9.26EE260 pKa = 4.22LQHH263 pKa = 6.64GPLGDD268 pKa = 3.84PPRR271 pKa = 11.84LRR273 pKa = 11.84SGRR276 pKa = 11.84HH277 pKa = 4.81LRR279 pKa = 11.84TFGIVFQRR287 pKa = 11.84VFQRR291 pKa = 11.84VFQRR295 pKa = 11.84VSRR298 pKa = 11.84RR299 pKa = 11.84RR300 pKa = 11.84FGGCFGGTSAVPRR313 pKa = 11.84RR314 pKa = 11.84NVVQEE319 pKa = 3.96SFNVHH324 pKa = 6.78DD325 pKa = 4.76EE326 pKa = 4.3SEE328 pKa = 4.06KK329 pKa = 10.86DD330 pKa = 3.54SRR332 pKa = 11.84GMCPSRR338 pKa = 11.84TVRR341 pKa = 11.84PFQKK345 pKa = 10.14VSHH348 pKa = 6.59LSGGEE353 pKa = 3.5RR354 pKa = 11.84WEE356 pKa = 4.06RR357 pKa = 11.84NYY359 pKa = 10.78PLLFCTLCDD368 pKa = 3.56QLEE371 pKa = 4.23LLGVQQ376 pKa = 3.94

Molecular weight: 41.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

96

1

97

30409

49

1293

313.5

34.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.586 ± 0.227

2.013 ± 0.129

6.123 ± 0.168

5.748 ± 0.18

3.265 ± 0.14

7.764 ± 0.379

2.161 ± 0.146

3.242 ± 0.135

5.462 ± 0.234

7.929 ± 0.274

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.068 ± 0.134

2.568 ± 0.13

6.242 ± 0.304

2.933 ± 0.243

7.001 ± 0.265

7.159 ± 0.205

5.725 ± 0.164

8.603 ± 0.225

1.388 ± 0.095

3.019 ± 0.147
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski