Pseudomonas phage MR1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Ghunavirus; unclassified Ghunavirus

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 50 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M3T8H4|A0A6M3T8H4_9CAUD Uncharacterized protein OS=Pseudomonas phage MR1 OX=2711169 GN=PssvBMR1_gp13 PE=4 SV=1
MM1 pKa = 5.9VTAMGLNRR9 pKa = 11.84EE10 pKa = 4.22QKK12 pKa = 10.46DD13 pKa = 2.94WAAQHH18 pKa = 6.18DD19 pKa = 4.53WYY21 pKa = 11.13RR22 pKa = 11.84EE23 pKa = 4.18CFVNAYY29 pKa = 10.17NLYY32 pKa = 8.89TVMVYY37 pKa = 10.51DD38 pKa = 4.75SEE40 pKa = 5.4LDD42 pKa = 3.6DD43 pKa = 5.06VIPMTDD49 pKa = 3.76FNEE52 pKa = 3.81LRR54 pKa = 11.84SWAGYY59 pKa = 9.65

Molecular weight: 7.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M3TA20|A0A6M3TA20_9CAUD Putative single-stranded DNA-binding protein OS=Pseudomonas phage MR1 OX=2711169 GN=PssvBMR1_gp16 PE=4 SV=1
MM1 pKa = 7.46FARR4 pKa = 11.84NFEE7 pKa = 4.14KK8 pKa = 8.7TTRR11 pKa = 11.84TNARR15 pKa = 11.84RR16 pKa = 11.84SFEE19 pKa = 4.04EE20 pKa = 4.14VEE22 pKa = 3.81QARR25 pKa = 11.84AKK27 pKa = 10.2KK28 pKa = 10.43GKK30 pKa = 9.22SNKK33 pKa = 9.11PNRR36 pKa = 11.84TQRR39 pKa = 11.84TEE41 pKa = 3.34WEE43 pKa = 4.4TNN45 pKa = 3.11

Molecular weight: 5.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

50

0

50

12442

30

1399

248.8

27.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.749 ± 0.472

0.892 ± 0.145

6.454 ± 0.266

6.382 ± 0.33

3.561 ± 0.187

7.997 ± 0.275

1.841 ± 0.176

4.822 ± 0.187

5.578 ± 0.291

7.957 ± 0.331

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.934 ± 0.125

4.372 ± 0.26

3.922 ± 0.221

4.445 ± 0.322

5.875 ± 0.221

6.301 ± 0.368

5.859 ± 0.251

6.566 ± 0.29

1.463 ± 0.14

3.03 ± 0.142
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski