Mycoplasma sp. CAG:472

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Mycoplasmatales; Mycoplasmataceae; Mycoplasma; environmental samples

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1281 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R7HHB3|R7HHB3_9MOLU ABC2_membrane domain-containing protein OS=Mycoplasma sp. CAG:472 OX=1262904 GN=BN672_00905 PE=4 SV=1
MM1 pKa = 7.06YY2 pKa = 9.77NARR5 pKa = 11.84VDD7 pKa = 3.57KK8 pKa = 11.07SAGLDD13 pKa = 3.3DD14 pKa = 4.47VMWIVDD20 pKa = 5.0MICDD24 pKa = 4.41DD25 pKa = 4.93PDD27 pKa = 3.62LVDD30 pKa = 4.37YY31 pKa = 10.82IQEE34 pKa = 4.05EE35 pKa = 4.8TIKK38 pKa = 10.96SLEE41 pKa = 3.99DD42 pKa = 3.87DD43 pKa = 3.96YY44 pKa = 11.95NIILNDD50 pKa = 3.39IQII53 pKa = 3.89

Molecular weight: 6.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R7HGZ1|R7HGZ1_9MOLU Swim zinc finger domain protein OS=Mycoplasma sp. CAG:472 OX=1262904 GN=BN672_00784 PE=4 SV=1
MM1 pKa = 7.48SNSRR5 pKa = 11.84SNARR9 pKa = 11.84TCKK12 pKa = 10.52AKK14 pKa = 10.57ARR16 pKa = 11.84NNARR20 pKa = 11.84SNSQSARR27 pKa = 11.84SNSTNARR34 pKa = 11.84ANSRR38 pKa = 11.84SKK40 pKa = 11.21

Molecular weight: 4.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1281

0

1281

370019

29

1974

288.9

33.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.092 ± 0.061

1.18 ± 0.029

6.073 ± 0.052

6.962 ± 0.069

4.621 ± 0.061

5.145 ± 0.072

1.232 ± 0.024

10.13 ± 0.085

10.295 ± 0.075

9.215 ± 0.074

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.312 ± 0.031

8.362 ± 0.078

2.355 ± 0.034

1.833 ± 0.035

2.654 ± 0.045

6.452 ± 0.057

5.172 ± 0.065

5.759 ± 0.048

0.482 ± 0.019

5.673 ± 0.056
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski