Lysinibacillus sinduriensis BLB-1 = JCM 15800

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Planococcaceae; Ureibacillus; Ureibacillus sinduriensis

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3540 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A3IR80|A0A0A3IR80_9BACI o-succinylbenzoate synthase OS=Lysinibacillus sinduriensis BLB-1 = JCM 15800 OX=1384057 GN=menC PE=3 SV=1
TT1 pKa = 7.17GPAGPTGPAGPTGPTVTEE19 pKa = 4.15EE20 pKa = 4.41GFSALAGAASVTGTSQITDD39 pKa = 3.3WTVTAPYY46 pKa = 7.88FTNGNFDD53 pKa = 4.31PATGTYY59 pKa = 7.19TAPVTGRR66 pKa = 11.84YY67 pKa = 9.48SISATISYY75 pKa = 7.6QTTAALTVSLGTGIDD90 pKa = 3.32PAFVVRR96 pKa = 11.84NITTGTDD103 pKa = 4.35LISGLLPVLNVNVALLLTLRR123 pKa = 11.84AVLGNGTVTLSGDD136 pKa = 3.67VEE138 pKa = 4.37LTAGDD143 pKa = 4.6DD144 pKa = 2.95IGLFYY149 pKa = 10.67EE150 pKa = 5.14ADD152 pKa = 3.6GLTIGLDD159 pKa = 3.32LGGPGVPIVWSIHH172 pKa = 5.84QITEE176 pKa = 4.1TLL178 pKa = 3.43

Molecular weight: 17.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A3HWG1|A0A0A3HWG1_9BACI Uncharacterized protein OS=Lysinibacillus sinduriensis BLB-1 = JCM 15800 OX=1384057 GN=CD33_04475 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.54QPKK8 pKa = 9.44KK9 pKa = 7.96RR10 pKa = 11.84KK11 pKa = 8.69HH12 pKa = 5.94SKK14 pKa = 8.54VHH16 pKa = 5.68GFRR19 pKa = 11.84ARR21 pKa = 11.84MSSKK25 pKa = 9.98NGRR28 pKa = 11.84RR29 pKa = 11.84VLAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.81GRR39 pKa = 11.84KK40 pKa = 8.75VLSAA44 pKa = 4.05

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3540

0

3540

1020967

34

1722

288.4

32.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.026 ± 0.046

0.697 ± 0.011

4.982 ± 0.03

7.745 ± 0.049

4.596 ± 0.037

6.718 ± 0.043

1.988 ± 0.017

8.271 ± 0.041

6.635 ± 0.038

9.829 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.72 ± 0.02

4.614 ± 0.033

3.439 ± 0.02

3.723 ± 0.028

3.907 ± 0.032

6.119 ± 0.033

5.495 ± 0.033

7.019 ± 0.034

0.958 ± 0.017

3.519 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski