Thecamonas trahens ATCC 50062

Taxonomy: cellular organisms; Eukaryota; Apusozoa; Apusomonadidae; Thecamonas; Thecamonas trahens

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10604 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0L0DW12|A0A0L0DW12_THETB DnaJ-domain-containing protein 1 OS=Thecamonas trahens ATCC 50062 OX=461836 GN=AMSG_02332 PE=3 SV=1
MM1 pKa = 7.61AVYY4 pKa = 9.89QVLTTMAMVCMLAHH18 pKa = 7.71LARR21 pKa = 11.84GDD23 pKa = 3.49GTTQLGTTQALQASTVLYY41 pKa = 10.92LDD43 pKa = 3.97IDD45 pKa = 4.16NIANQRR51 pKa = 11.84IYY53 pKa = 10.85FQGTGQLRR61 pKa = 11.84IYY63 pKa = 10.45RR64 pKa = 11.84PGDD67 pKa = 3.15FAQVGTILSGQLYY80 pKa = 7.89TPTMTGVYY88 pKa = 9.93GALLTLDD95 pKa = 3.44QTYY98 pKa = 10.3GAVWDD103 pKa = 3.59ISVYY107 pKa = 9.58DD108 pKa = 4.06TSTSSIDD115 pKa = 3.09AGRR118 pKa = 11.84VFSFSWNLASNSFSQSDD135 pKa = 3.8AVDD138 pKa = 3.42VSIWSLFSTSLGAKK152 pKa = 8.98LVEE155 pKa = 4.04NRR157 pKa = 11.84FEE159 pKa = 4.12GMAGFIYY166 pKa = 10.2QIAASTTGIEE176 pKa = 4.27TATPKK181 pKa = 10.56YY182 pKa = 10.51KK183 pKa = 10.5SVLQSGNSVTPNIRR197 pKa = 11.84LYY199 pKa = 10.66LSKK202 pKa = 10.36PEE204 pKa = 4.17HH205 pKa = 5.67VTLSSSVPNIVFEE218 pKa = 4.15GVEE221 pKa = 4.83FVTLGCFDD229 pKa = 5.89FISDD233 pKa = 4.32CNTVVADD240 pKa = 3.98KK241 pKa = 10.27VHH243 pKa = 6.53PRR245 pKa = 11.84FKK247 pKa = 10.86FNTDD251 pKa = 3.02AAGGRR256 pKa = 11.84YY257 pKa = 7.4TLVCDD262 pKa = 4.09TNSDD266 pKa = 3.51GALDD270 pKa = 4.46LVRR273 pKa = 11.84QTDD276 pKa = 3.3TSLRR280 pKa = 11.84GTTVAGDD287 pKa = 4.71NIVEE291 pKa = 3.94WDD293 pKa = 3.65GRR295 pKa = 11.84DD296 pKa = 3.56DD297 pKa = 4.17NNALVTAGFYY307 pKa = 7.36TCKK310 pKa = 9.84MFVYY314 pKa = 10.11LDD316 pKa = 4.45EE317 pKa = 4.21IHH319 pKa = 6.75FPLVDD324 pKa = 3.7MEE326 pKa = 4.51TVYY329 pKa = 10.86PGLRR333 pKa = 11.84MFDD336 pKa = 3.67HH337 pKa = 7.3SSGTPRR343 pKa = 11.84PQIMYY348 pKa = 9.88WNDD351 pKa = 3.33DD352 pKa = 3.61GVNAPIAMPNGQFGATYY369 pKa = 10.07SGCCGIDD376 pKa = 3.13SGVSTDD382 pKa = 4.35PISPNVNARR391 pKa = 11.84SWGNFVSGSKK401 pKa = 10.91GDD403 pKa = 3.7TAILNTFSLVEE414 pKa = 4.18VTVSEE419 pKa = 4.88AINISVAQPSQCEE432 pKa = 3.92TATVNLIDD440 pKa = 3.97YY441 pKa = 9.86VLTEE445 pKa = 4.74LDD447 pKa = 3.14SGTTVVEE454 pKa = 4.15VKK456 pKa = 10.56LAVSSYY462 pKa = 10.41LAGDD466 pKa = 3.08ITLAYY471 pKa = 6.2TTADD475 pKa = 3.34GTATAGSGDD484 pKa = 3.85YY485 pKa = 10.61VATSGSIVISSGDD498 pKa = 3.41FCNSLSVTINGDD510 pKa = 3.74DD511 pKa = 3.13IVEE514 pKa = 3.92YY515 pKa = 10.88DD516 pKa = 3.05EE517 pKa = 5.28WFFLDD522 pKa = 3.55VTQTAGPTVIIADD535 pKa = 3.3GRR537 pKa = 11.84ANVTILNDD545 pKa = 3.71DD546 pKa = 3.96TPVTISMTNSATSVTEE562 pKa = 3.97PSSGSANAGLTFTLSAPSTAPVTVYY587 pKa = 10.81LATTAPGGSLVTSGTDD603 pKa = 3.52FTPPPASIVIPAGVTTFTYY622 pKa = 9.99DD623 pKa = 2.75IPVAADD629 pKa = 3.79RR630 pKa = 11.84FWDD633 pKa = 4.12DD634 pKa = 3.81GEE636 pKa = 4.26QFTVSIAGTSVEE648 pKa = 4.31GAVGGQTSTTFTINDD663 pKa = 3.73SGPAPVVTASVVSGNVASSTGDD685 pKa = 3.15GTASLSSTLPGDD697 pKa = 3.68GRR699 pKa = 11.84VGEE702 pKa = 4.42SNEE705 pKa = 3.78VVYY708 pKa = 10.71QLTLSRR714 pKa = 11.84PSEE717 pKa = 4.11YY718 pKa = 10.77AAGLTLNVDD727 pKa = 4.38GPATTATLPGDD738 pKa = 3.87ADD740 pKa = 3.98LVGTIDD746 pKa = 3.42RR747 pKa = 11.84TGGPFTIAAGEE758 pKa = 4.12TSFNVSLVVAGDD770 pKa = 3.63GDD772 pKa = 4.04AEE774 pKa = 4.26ADD776 pKa = 3.63EE777 pKa = 5.33VVDD780 pKa = 5.12FDD782 pKa = 5.76AVVNLNGASNPTTTATILTNIPNDD806 pKa = 3.72DD807 pKa = 3.69VAVVQMAAPTAATTEE822 pKa = 4.41PGSGTVNVPITVNLLATSPSPVTINIQTSVSSGSLATGGADD863 pKa = 3.49FTAEE867 pKa = 4.06STTVVVPPGQMSVVYY882 pKa = 9.52NAVVAADD889 pKa = 3.22TWYY892 pKa = 10.51DD893 pKa = 3.23GGEE896 pKa = 4.15TFDD899 pKa = 4.23VKK901 pKa = 10.25ITGADD906 pKa = 2.85NGFAVGAADD915 pKa = 3.69TTVVTIADD923 pKa = 3.82SGPAPVLTATVVGGTTTAVSGDD945 pKa = 3.66GTDD948 pKa = 3.89VIDD951 pKa = 3.54VTGPEE956 pKa = 4.44GAASTTNEE964 pKa = 4.13LVVEE968 pKa = 4.6LALSRR973 pKa = 11.84PSEE976 pKa = 4.14YY977 pKa = 10.79AAGLTVQVDD986 pKa = 4.6GVTSTADD993 pKa = 3.35VPSDD997 pKa = 3.24ATIVDD1002 pKa = 4.03TVARR1006 pKa = 11.84TGSPMTIPALATTHH1020 pKa = 5.12QVSVVVSGDD1029 pKa = 3.27GVLEE1033 pKa = 4.02PTEE1036 pKa = 4.16VLDD1039 pKa = 5.31LGLIANYY1046 pKa = 10.3GGAEE1050 pKa = 3.96LAADD1054 pKa = 4.07VVSVTATITNDD1065 pKa = 3.12DD1066 pKa = 3.91AAVVTMAAGTAAATEE1081 pKa = 4.5PGSGTVNVPITQALRR1096 pKa = 3.48

Molecular weight: 112.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0L0DL00|A0A0L0DL00_THETB Lactamase_B domain-containing protein OS=Thecamonas trahens ATCC 50062 OX=461836 GN=AMSG_11629 PE=4 SV=1
MM1 pKa = 6.61PTARR5 pKa = 11.84ALSTRR10 pKa = 11.84PSSGSSSATPSRR22 pKa = 11.84PASRR26 pKa = 11.84SRR28 pKa = 11.84TRR30 pKa = 11.84IRR32 pKa = 11.84RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84QKK38 pKa = 9.8RR39 pKa = 11.84ARR41 pKa = 11.84ARR43 pKa = 11.84ARR45 pKa = 11.84EE46 pKa = 4.03PARR49 pKa = 11.84AQARR53 pKa = 11.84RR54 pKa = 11.84QRR56 pKa = 11.84HH57 pKa = 3.55RR58 pKa = 11.84RR59 pKa = 11.84RR60 pKa = 11.84RR61 pKa = 11.84RR62 pKa = 11.84KK63 pKa = 6.37QTRR66 pKa = 11.84HH67 pKa = 4.32RR68 pKa = 11.84TQPQPRR74 pKa = 11.84LRR76 pKa = 11.84ARR78 pKa = 11.84ARR80 pKa = 11.84PVARR84 pKa = 11.84TTSIAALRR92 pKa = 11.84PGRR95 pKa = 11.84SWPTRR100 pKa = 11.84RR101 pKa = 11.84GWKK104 pKa = 9.87RR105 pKa = 11.84PVTRR109 pKa = 11.84RR110 pKa = 11.84SAGMTRR116 pKa = 11.84RR117 pKa = 11.84KK118 pKa = 9.85GRR120 pKa = 11.84RR121 pKa = 11.84ADD123 pKa = 3.18AA124 pKa = 4.43

Molecular weight: 14.57 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10533

71

10604

6918374

49

16619

652.4

69.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.527 ± 0.05

1.201 ± 0.014

5.752 ± 0.017

5.574 ± 0.036

3.012 ± 0.017

7.239 ± 0.032

2.153 ± 0.011

3.264 ± 0.015

3.295 ± 0.025

9.859 ± 0.029

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.042 ± 0.01

2.349 ± 0.012

5.7 ± 0.029

2.654 ± 0.016

6.259 ± 0.026

8.543 ± 0.029

5.407 ± 0.025

7.915 ± 0.028

1.122 ± 0.009

2.134 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski