Anopheles stephensi (Indo-Pakistan malaria mosquito)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Endopterygota; Diptera;

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11765 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A182XYE5|A0A182XYE5_ANOST Uncharacterized protein OS=Anopheles stephensi OX=30069 PE=4 SV=1
MM1 pKa = 7.51CCAPMSDD8 pKa = 4.31YY9 pKa = 9.03CTKK12 pKa = 10.15MALLDD17 pKa = 3.87DD18 pKa = 4.44TNGNTVNINNQPSGEE33 pKa = 4.19AGPGDD38 pKa = 3.8GGGGGMAPATGDD50 pKa = 3.8SVTMLHH56 pKa = 6.54SPSNDD61 pKa = 3.33DD62 pKa = 3.76LSQSLSEE69 pKa = 4.12YY70 pKa = 10.0TDD72 pKa = 3.56ADD74 pKa = 3.78EE75 pKa = 5.73SISAPTEE82 pKa = 3.6LLAEE86 pKa = 4.23FLSAIMIRR94 pKa = 11.84DD95 pKa = 3.45YY96 pKa = 11.94VNALKK101 pKa = 10.6YY102 pKa = 10.31CKK104 pKa = 9.93QILQYY109 pKa = 10.68EE110 pKa = 4.53PNNTTARR117 pKa = 11.84DD118 pKa = 4.66FYY120 pKa = 10.89PHH122 pKa = 7.03ILSKK126 pKa = 10.41ISEE129 pKa = 4.26MQNNEE134 pKa = 3.57LSGEE138 pKa = 3.91SDD140 pKa = 2.95EE141 pKa = 5.59NYY143 pKa = 10.76NFNCSSPLASSASSPSSCSVSSSPGSSRR171 pKa = 11.84SSRR174 pKa = 11.84SGSSSSSSSGCDD186 pKa = 3.27NIDD189 pKa = 3.46EE190 pKa = 4.72VIMVCSSDD198 pKa = 3.39SDD200 pKa = 3.78EE201 pKa = 5.07SSEE204 pKa = 4.39ACCSLPDD211 pKa = 4.39SVGDD215 pKa = 3.69EE216 pKa = 4.24AKK218 pKa = 10.72GEE220 pKa = 4.08PRR222 pKa = 11.84SEE224 pKa = 4.06PVGEE228 pKa = 4.17RR229 pKa = 11.84QVVTAVGAIPATGGSRR245 pKa = 11.84SLSDD249 pKa = 3.79ANTSQSYY256 pKa = 11.1SSLLLEE262 pKa = 5.6DD263 pKa = 4.09EE264 pKa = 4.9EE265 pKa = 6.15KK266 pKa = 11.13DD267 pKa = 3.51LTLSDD272 pKa = 3.41ISNLNIEE279 pKa = 4.45EE280 pKa = 4.98DD281 pKa = 3.82EE282 pKa = 4.35TSNDD286 pKa = 3.46CGGSLEE292 pKa = 4.38NSYY295 pKa = 9.63TPLDD299 pKa = 3.76VSAPPVVKK307 pKa = 10.35PPVPTTNLASATVFASRR324 pKa = 11.84IVAMLRR330 pKa = 11.84AKK332 pKa = 9.96VIPSKK337 pKa = 10.87AAGDD341 pKa = 3.69

Molecular weight: 35.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A182XV26|A0A182XV26_ANOST 39S ribosomal protein L34 mitochondrial OS=Anopheles stephensi OX=30069 PE=3 SV=1
LL1 pKa = 7.48SRR3 pKa = 11.84LPLLPMLPRR12 pKa = 11.84LPTLTIKK19 pKa = 9.09EE20 pKa = 4.55TTSRR24 pKa = 11.84PSRR27 pKa = 11.84LSRR30 pKa = 11.84PSTLRR35 pKa = 11.84SRR37 pKa = 11.84RR38 pKa = 11.84KK39 pKa = 9.48LAMWPPTVVLSMRR52 pKa = 11.84LLWPVMPSTRR62 pKa = 11.84SRR64 pKa = 3.28

Molecular weight: 7.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11765

0

11765

6746010

23

12716

573.4

63.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.411 ± 0.021

1.849 ± 0.028

5.339 ± 0.018

6.253 ± 0.028

3.505 ± 0.02

6.656 ± 0.034

2.702 ± 0.015

4.876 ± 0.021

5.27 ± 0.024

8.909 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.279 ± 0.01

4.5 ± 0.017

5.452 ± 0.026

4.782 ± 0.032

5.631 ± 0.021

8.215 ± 0.038

6.085 ± 0.019

6.263 ± 0.02

0.971 ± 0.007

3.05 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski